Amino acid dipepetide frequency for Pseudomonas phage phiPMW

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.545AlaAla: 6.545 ± 0.714
0.939AlaCys: 0.939 ± 0.154
3.789AlaAsp: 3.789 ± 0.418
4.697AlaGlu: 4.697 ± 0.393
2.881AlaPhe: 2.881 ± 0.332
4.854AlaGly: 4.854 ± 0.397
1.66AlaHis: 1.66 ± 0.23
4.917AlaIle: 4.917 ± 0.473
4.51AlaLys: 4.51 ± 0.465
6.89AlaLeu: 6.89 ± 0.518
2.787AlaMet: 2.787 ± 0.262
3.069AlaAsn: 3.069 ± 0.394
2.004AlaPro: 2.004 ± 0.25
3.038AlaGln: 3.038 ± 0.391
3.288AlaArg: 3.288 ± 0.314
4.541AlaSer: 4.541 ± 0.392
5.637AlaThr: 5.637 ± 0.711
5.324AlaVal: 5.324 ± 0.484
1.096AlaTrp: 1.096 ± 0.18
2.599AlaTyr: 2.599 ± 0.279
0.0AlaXaa: 0.0 ± 0.0
Cys
0.626CysAla: 0.626 ± 0.131
0.407CysCys: 0.407 ± 0.103
0.908CysAsp: 0.908 ± 0.187
1.19CysGlu: 1.19 ± 0.2
0.501CysPhe: 0.501 ± 0.161
1.19CysGly: 1.19 ± 0.189
0.282CysHis: 0.282 ± 0.097
0.814CysIle: 0.814 ± 0.171
0.752CysLys: 0.752 ± 0.186
0.971CysLeu: 0.971 ± 0.182
0.532CysMet: 0.532 ± 0.14
0.532CysAsn: 0.532 ± 0.121
0.689CysPro: 0.689 ± 0.157
0.282CysGln: 0.282 ± 0.134
0.595CysArg: 0.595 ± 0.152
0.908CysSer: 0.908 ± 0.166
0.595CysThr: 0.595 ± 0.151
0.846CysVal: 0.846 ± 0.2
0.219CysTrp: 0.219 ± 0.07
0.564CysTyr: 0.564 ± 0.143
0.0CysXaa: 0.0 ± 0.0
Asp
4.165AspAla: 4.165 ± 0.408
1.033AspCys: 1.033 ± 0.171
4.102AspAsp: 4.102 ± 0.355
4.76AspGlu: 4.76 ± 0.506
3.132AspPhe: 3.132 ± 0.342
6.075AspGly: 6.075 ± 0.507
0.971AspHis: 0.971 ± 0.186
3.695AspIle: 3.695 ± 0.325
3.288AspLys: 3.288 ± 0.283
4.791AspLeu: 4.791 ± 0.418
2.38AspMet: 2.38 ± 0.284
3.32AspAsn: 3.32 ± 0.311
2.317AspPro: 2.317 ± 0.267
1.441AspGln: 1.441 ± 0.218
2.944AspArg: 2.944 ± 0.3
3.226AspSer: 3.226 ± 0.333
3.445AspThr: 3.445 ± 0.347
3.883AspVal: 3.883 ± 0.437
1.691AspTrp: 1.691 ± 0.28
2.787AspTyr: 2.787 ± 0.306
0.0AspXaa: 0.0 ± 0.0
Glu
6.169GluAla: 6.169 ± 0.597
1.347GluCys: 1.347 ± 0.241
3.946GluAsp: 3.946 ± 0.368
5.95GluGlu: 5.95 ± 0.564
3.445GluPhe: 3.445 ± 0.406
4.541GluGly: 4.541 ± 0.419
1.848GluHis: 1.848 ± 0.284
4.697GluIle: 4.697 ± 0.387
4.791GluLys: 4.791 ± 0.385
6.075GluLeu: 6.075 ± 0.459
2.505GluMet: 2.505 ± 0.283
2.881GluAsn: 2.881 ± 0.286
1.66GluPro: 1.66 ± 0.237
3.414GluGln: 3.414 ± 0.386
3.163GluArg: 3.163 ± 0.335
3.351GluSer: 3.351 ± 0.283
3.883GluThr: 3.883 ± 0.412
5.292GluVal: 5.292 ± 0.442
1.378GluTrp: 1.378 ± 0.201
2.881GluTyr: 2.881 ± 0.267
0.0GluXaa: 0.0 ± 0.0
Phe
2.818PheAla: 2.818 ± 0.291
0.564PheCys: 0.564 ± 0.126
3.507PheAsp: 3.507 ± 0.3
3.57PheGlu: 3.57 ± 0.353
1.535PhePhe: 1.535 ± 0.245
3.038PheGly: 3.038 ± 0.282
0.877PheHis: 0.877 ± 0.186
2.13PheIle: 2.13 ± 0.267
2.85PheLys: 2.85 ± 0.296
2.725PheLeu: 2.725 ± 0.35
1.566PheMet: 1.566 ± 0.336
2.161PheAsn: 2.161 ± 0.266
1.253PhePro: 1.253 ± 0.212
1.66PheGln: 1.66 ± 0.216
1.378PheArg: 1.378 ± 0.196
2.411PheSer: 2.411 ± 0.331
2.725PheThr: 2.725 ± 0.282
2.443PheVal: 2.443 ± 0.296
0.47PheTrp: 0.47 ± 0.117
1.315PheTyr: 1.315 ± 0.192
0.0PheXaa: 0.0 ± 0.0
Gly
4.009GlyAla: 4.009 ± 0.401
1.033GlyCys: 1.033 ± 0.18
4.447GlyAsp: 4.447 ± 0.351
5.449GlyGlu: 5.449 ± 0.386
3.288GlyPhe: 3.288 ± 0.429
5.668GlyGly: 5.668 ± 0.607
1.754GlyHis: 1.754 ± 0.248
4.478GlyIle: 4.478 ± 0.317
5.042GlyLys: 5.042 ± 0.486
5.042GlyLeu: 5.042 ± 0.492
3.069GlyMet: 3.069 ± 0.309
3.382GlyAsn: 3.382 ± 0.331
1.535GlyPro: 1.535 ± 0.212
2.349GlyGln: 2.349 ± 0.289
3.006GlyArg: 3.006 ± 0.293
4.165GlySer: 4.165 ± 0.355
4.228GlyThr: 4.228 ± 0.477
4.885GlyVal: 4.885 ± 0.395
1.315GlyTrp: 1.315 ± 0.182
3.382GlyTyr: 3.382 ± 0.381
0.0GlyXaa: 0.0 ± 0.0
His
1.91HisAla: 1.91 ± 0.294
0.282HisCys: 0.282 ± 0.122
1.628HisAsp: 1.628 ± 0.278
1.347HisGlu: 1.347 ± 0.21
0.783HisPhe: 0.783 ± 0.161
1.785HisGly: 1.785 ± 0.288
0.532HisHis: 0.532 ± 0.154
1.284HisIle: 1.284 ± 0.205
1.722HisLys: 1.722 ± 0.229
1.284HisLeu: 1.284 ± 0.213
0.532HisMet: 0.532 ± 0.132
0.783HisAsn: 0.783 ± 0.174
1.033HisPro: 1.033 ± 0.211
0.532HisGln: 0.532 ± 0.127
0.72HisArg: 0.72 ± 0.159
1.472HisSer: 1.472 ± 0.219
1.441HisThr: 1.441 ± 0.231
1.253HisVal: 1.253 ± 0.202
0.532HisTrp: 0.532 ± 0.153
1.065HisTyr: 1.065 ± 0.199
0.0HisXaa: 0.0 ± 0.0
Ile
5.042IleAla: 5.042 ± 0.383
0.846IleCys: 0.846 ± 0.195
5.167IleAsp: 5.167 ± 0.392
4.979IleGlu: 4.979 ± 0.402
1.221IlePhe: 1.221 ± 0.177
4.071IleGly: 4.071 ± 0.389
1.409IleHis: 1.409 ± 0.205
2.912IleIle: 2.912 ± 0.315
3.664IleLys: 3.664 ± 0.377
4.29IleLeu: 4.29 ± 0.475
1.628IleMet: 1.628 ± 0.221
2.944IleAsn: 2.944 ± 0.327
2.004IlePro: 2.004 ± 0.29
2.13IleGln: 2.13 ± 0.225
2.474IleArg: 2.474 ± 0.296
2.662IleSer: 2.662 ± 0.3
4.009IleThr: 4.009 ± 0.441
3.977IleVal: 3.977 ± 0.339
0.532IleTrp: 0.532 ± 0.145
2.067IleTyr: 2.067 ± 0.259
0.0IleXaa: 0.0 ± 0.0
Lys
4.885LysAla: 4.885 ± 0.452
0.626LysCys: 0.626 ± 0.154
3.758LysAsp: 3.758 ± 0.413
4.885LysGlu: 4.885 ± 0.44
2.881LysPhe: 2.881 ± 0.288
4.76LysGly: 4.76 ± 0.358
1.566LysHis: 1.566 ± 0.254
3.351LysIle: 3.351 ± 0.326
3.132LysLys: 3.132 ± 0.39
5.167LysLeu: 5.167 ± 0.464
2.411LysMet: 2.411 ± 0.319
2.599LysAsn: 2.599 ± 0.336
1.785LysPro: 1.785 ± 0.282
2.944LysGln: 2.944 ± 0.314
3.006LysArg: 3.006 ± 0.368
3.414LysSer: 3.414 ± 0.359
3.382LysThr: 3.382 ± 0.37
4.979LysVal: 4.979 ± 0.377
1.284LysTrp: 1.284 ± 0.217
2.411LysTyr: 2.411 ± 0.273
0.0LysXaa: 0.0 ± 0.0
Leu
5.261LeuAla: 5.261 ± 0.416
1.127LeuCys: 1.127 ± 0.188
4.447LeuAsp: 4.447 ± 0.372
5.105LeuGlu: 5.105 ± 0.43
2.912LeuPhe: 2.912 ± 0.383
4.823LeuGly: 4.823 ± 0.37
1.816LeuHis: 1.816 ± 0.271
4.384LeuIle: 4.384 ± 0.435
5.199LeuLys: 5.199 ± 0.431
4.635LeuLeu: 4.635 ± 0.477
2.505LeuMet: 2.505 ± 0.256
3.727LeuAsn: 3.727 ± 0.375
2.537LeuPro: 2.537 ± 0.284
2.912LeuGln: 2.912 ± 0.349
3.476LeuArg: 3.476 ± 0.314
5.073LeuSer: 5.073 ± 0.396
6.42LeuThr: 6.42 ± 0.527
4.791LeuVal: 4.791 ± 0.392
1.221LeuTrp: 1.221 ± 0.223
2.756LeuTyr: 2.756 ± 0.256
0.0LeuXaa: 0.0 ± 0.0
Met
2.85MetAla: 2.85 ± 0.324
0.344MetCys: 0.344 ± 0.102
1.848MetAsp: 1.848 ± 0.243
2.223MetGlu: 2.223 ± 0.259
1.19MetPhe: 1.19 ± 0.192
1.566MetGly: 1.566 ± 0.224
0.532MetHis: 0.532 ± 0.117
2.067MetIle: 2.067 ± 0.271
2.161MetLys: 2.161 ± 0.262
2.599MetLeu: 2.599 ± 0.333
0.939MetMet: 0.939 ± 0.223
1.942MetAsn: 1.942 ± 0.258
1.19MetPro: 1.19 ± 0.179
1.315MetGln: 1.315 ± 0.24
1.785MetArg: 1.785 ± 0.271
2.411MetSer: 2.411 ± 0.267
2.098MetThr: 2.098 ± 0.234
2.223MetVal: 2.223 ± 0.254
0.595MetTrp: 0.595 ± 0.167
1.096MetTyr: 1.096 ± 0.208
0.0MetXaa: 0.0 ± 0.0
Asn
4.322AsnAla: 4.322 ± 0.423
0.47AsnCys: 0.47 ± 0.163
2.85AsnAsp: 2.85 ± 0.307
3.382AsnGlu: 3.382 ± 0.34
1.503AsnPhe: 1.503 ± 0.192
3.977AsnGly: 3.977 ± 0.342
1.284AsnHis: 1.284 ± 0.216
3.1AsnIle: 3.1 ± 0.323
3.226AsnLys: 3.226 ± 0.334
3.476AsnLeu: 3.476 ± 0.312
1.503AsnMet: 1.503 ± 0.216
2.098AsnAsn: 2.098 ± 0.34
2.004AsnPro: 2.004 ± 0.226
2.067AsnGln: 2.067 ± 0.319
1.879AsnArg: 1.879 ± 0.244
2.255AsnSer: 2.255 ± 0.294
2.944AsnThr: 2.944 ± 0.384
2.912AsnVal: 2.912 ± 0.319
0.626AsnTrp: 0.626 ± 0.148
1.378AsnTyr: 1.378 ± 0.219
0.0AsnXaa: 0.0 ± 0.0
Pro
2.161ProAla: 2.161 ± 0.307
0.376ProCys: 0.376 ± 0.124
2.599ProAsp: 2.599 ± 0.254
2.568ProGlu: 2.568 ± 0.312
1.879ProPhe: 1.879 ± 0.27
1.441ProGly: 1.441 ± 0.193
0.72ProHis: 0.72 ± 0.158
1.628ProIle: 1.628 ± 0.264
2.161ProLys: 2.161 ± 0.28
2.036ProLeu: 2.036 ± 0.221
0.752ProMet: 0.752 ± 0.185
1.409ProAsn: 1.409 ± 0.184
1.127ProPro: 1.127 ± 0.221
1.19ProGln: 1.19 ± 0.192
1.347ProArg: 1.347 ± 0.195
2.004ProSer: 2.004 ± 0.257
2.004ProThr: 2.004 ± 0.257
2.599ProVal: 2.599 ± 0.325
0.595ProTrp: 0.595 ± 0.142
1.472ProTyr: 1.472 ± 0.242
0.0ProXaa: 0.0 ± 0.0
Gln
3.507GlnAla: 3.507 ± 0.423
0.501GlnCys: 0.501 ± 0.116
1.973GlnAsp: 1.973 ± 0.253
2.098GlnGlu: 2.098 ± 0.355
1.535GlnPhe: 1.535 ± 0.198
2.85GlnGly: 2.85 ± 0.281
0.783GlnHis: 0.783 ± 0.155
2.317GlnIle: 2.317 ± 0.278
2.255GlnLys: 2.255 ± 0.242
3.257GlnLeu: 3.257 ± 0.317
1.535GlnMet: 1.535 ± 0.257
1.785GlnAsn: 1.785 ± 0.214
0.877GlnPro: 0.877 ± 0.169
1.691GlnGln: 1.691 ± 0.269
2.693GlnArg: 2.693 ± 0.346
1.91GlnSer: 1.91 ± 0.237
1.848GlnThr: 1.848 ± 0.436
2.286GlnVal: 2.286 ± 0.296
0.72GlnTrp: 0.72 ± 0.153
1.973GlnTyr: 1.973 ± 0.24
0.0GlnXaa: 0.0 ± 0.0
Arg
2.944ArgAla: 2.944 ± 0.338
0.407ArgCys: 0.407 ± 0.116
2.787ArgAsp: 2.787 ± 0.279
3.226ArgGlu: 3.226 ± 0.364
2.067ArgPhe: 2.067 ± 0.214
2.756ArgGly: 2.756 ± 0.34
1.19ArgHis: 1.19 ± 0.23
2.975ArgIle: 2.975 ± 0.347
3.288ArgLys: 3.288 ± 0.408
3.821ArgLeu: 3.821 ± 0.305
1.628ArgMet: 1.628 ± 0.214
2.192ArgAsn: 2.192 ± 0.28
1.441ArgPro: 1.441 ± 0.195
2.317ArgGln: 2.317 ± 0.226
2.13ArgArg: 2.13 ± 0.257
2.13ArgSer: 2.13 ± 0.26
2.85ArgThr: 2.85 ± 0.29
3.915ArgVal: 3.915 ± 0.411
0.783ArgTrp: 0.783 ± 0.173
2.067ArgTyr: 2.067 ± 0.241
0.0ArgXaa: 0.0 ± 0.0
Ser
4.322SerAla: 4.322 ± 0.457
0.626SerCys: 0.626 ± 0.127
3.695SerAsp: 3.695 ± 0.38
3.476SerGlu: 3.476 ± 0.301
2.787SerPhe: 2.787 ± 0.332
4.791SerGly: 4.791 ± 0.448
0.939SerHis: 0.939 ± 0.208
3.476SerIle: 3.476 ± 0.287
3.163SerLys: 3.163 ± 0.303
4.322SerLeu: 4.322 ± 0.314
1.409SerMet: 1.409 ± 0.21
2.631SerAsn: 2.631 ± 0.327
2.223SerPro: 2.223 ± 0.315
2.13SerGln: 2.13 ± 0.257
2.505SerArg: 2.505 ± 0.261
3.288SerSer: 3.288 ± 0.394
3.633SerThr: 3.633 ± 0.363
3.507SerVal: 3.507 ± 0.381
1.002SerTrp: 1.002 ± 0.179
2.38SerTyr: 2.38 ± 0.233
0.0SerXaa: 0.0 ± 0.0
Thr
4.572ThrAla: 4.572 ± 0.613
0.689ThrCys: 0.689 ± 0.188
3.915ThrAsp: 3.915 ± 0.332
4.353ThrGlu: 4.353 ± 0.421
2.756ThrPhe: 2.756 ± 0.292
4.635ThrGly: 4.635 ± 0.426
1.472ThrHis: 1.472 ± 0.24
3.445ThrIle: 3.445 ± 0.365
3.915ThrLys: 3.915 ± 0.4
4.948ThrLeu: 4.948 ± 0.472
1.754ThrMet: 1.754 ± 0.274
3.069ThrAsn: 3.069 ± 0.626
1.628ThrPro: 1.628 ± 0.284
2.411ThrGln: 2.411 ± 0.308
3.194ThrArg: 3.194 ± 0.282
4.165ThrSer: 4.165 ± 0.494
3.915ThrThr: 3.915 ± 0.705
5.199ThrVal: 5.199 ± 0.646
0.846ThrTrp: 0.846 ± 0.148
2.85ThrTyr: 2.85 ± 0.27
0.0ThrXaa: 0.0 ± 0.0
Val
5.199ValAla: 5.199 ± 0.449
0.814ValCys: 0.814 ± 0.179
4.165ValAsp: 4.165 ± 0.354
5.199ValGlu: 5.199 ± 0.43
2.474ValPhe: 2.474 ± 0.313
4.791ValGly: 4.791 ± 0.388
1.159ValHis: 1.159 ± 0.24
3.539ValIle: 3.539 ± 0.36
4.76ValLys: 4.76 ± 0.351
5.073ValLeu: 5.073 ± 0.398
2.036ValMet: 2.036 ± 0.251
3.57ValAsn: 3.57 ± 0.45
2.38ValPro: 2.38 ± 0.328
2.349ValGln: 2.349 ± 0.287
4.134ValArg: 4.134 ± 0.368
3.695ValSer: 3.695 ± 0.363
5.105ValThr: 5.105 ± 0.525
5.574ValVal: 5.574 ± 0.46
0.72ValTrp: 0.72 ± 0.168
2.787ValTyr: 2.787 ± 0.264
0.0ValXaa: 0.0 ± 0.0
Trp
0.846TrpAla: 0.846 ± 0.165
0.313TrpCys: 0.313 ± 0.099
0.939TrpAsp: 0.939 ± 0.181
1.597TrpGlu: 1.597 ± 0.208
0.72TrpPhe: 0.72 ± 0.154
1.253TrpGly: 1.253 ± 0.193
0.282TrpHis: 0.282 ± 0.1
1.065TrpIle: 1.065 ± 0.192
0.908TrpLys: 0.908 ± 0.143
1.253TrpLeu: 1.253 ± 0.238
0.532TrpMet: 0.532 ± 0.126
0.72TrpAsn: 0.72 ± 0.15
0.783TrpPro: 0.783 ± 0.168
0.689TrpGln: 0.689 ± 0.169
0.814TrpArg: 0.814 ± 0.162
0.908TrpSer: 0.908 ± 0.158
0.814TrpThr: 0.814 ± 0.19
1.127TrpVal: 1.127 ± 0.195
0.407TrpTrp: 0.407 ± 0.127
0.689TrpTyr: 0.689 ± 0.145
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.912TyrAla: 2.912 ± 0.329
0.658TyrCys: 0.658 ± 0.156
2.975TyrAsp: 2.975 ± 0.352
3.163TyrGlu: 3.163 ± 0.341
1.503TyrPhe: 1.503 ± 0.239
2.662TyrGly: 2.662 ± 0.298
0.877TyrHis: 0.877 ± 0.154
1.754TyrIle: 1.754 ± 0.259
2.443TyrLys: 2.443 ± 0.358
2.474TyrLeu: 2.474 ± 0.261
0.939TyrMet: 0.939 ± 0.198
2.443TyrAsn: 2.443 ± 0.252
1.597TyrPro: 1.597 ± 0.242
1.535TyrGln: 1.535 ± 0.245
2.38TyrArg: 2.38 ± 0.271
2.317TyrSer: 2.317 ± 0.245
2.756TyrThr: 2.756 ± 0.292
2.505TyrVal: 2.505 ± 0.248
0.626TyrTrp: 0.626 ± 0.138
1.691TyrTyr: 1.691 ± 0.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 229 proteins (31933 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski