Eimeria mitis

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia; Eucoccidiorida; Eimeriorina; Eimeriidae; Eimeria

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10057 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U6KB67|U6KB67_9EIME Uncharacterized protein OS=Eimeria mitis OX=44415 GN=EMH_0024970 PE=4 SV=1
MM1 pKa = 8.04ADD3 pKa = 3.78PYY5 pKa = 10.46LQEE8 pKa = 4.4SSTVDD13 pKa = 2.78AWLLQQIIDD22 pKa = 4.19SPSVPLPGWTAQHH35 pKa = 6.7AGDD38 pKa = 3.89GQYY41 pKa = 10.91PEE43 pKa = 5.12DD44 pKa = 4.8WIDD47 pKa = 3.6ADD49 pKa = 4.03TDD51 pKa = 3.41QGSAQLTVADD61 pKa = 4.12TASNPGMLTSLSSQSQSSGGKK82 pKa = 8.69WEE84 pKa = 4.05THH86 pKa = 5.63PDD88 pKa = 2.82EE89 pKa = 4.73WPAVLHH95 pKa = 5.42QNPYY99 pKa = 10.95ADD101 pKa = 4.21LSSTSEE107 pKa = 3.93IPGSVGDD114 pKa = 3.76QLQSFGGEE122 pKa = 3.94WQPQTGSWPVQVYY135 pKa = 9.26PDD137 pKa = 3.48PHH139 pKa = 7.15RR140 pKa = 11.84GSLAAA145 pKa = 5.24

Molecular weight: 15.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U6K021|U6K021_9EIME Proton-translocating NAD(P)(+) transhydrogenase OS=Eimeria mitis OX=44415 GN=EMH_0003000 PE=4 SV=1
MM1 pKa = 7.53ISSSSSSSSSSGSSSSSSSSSNRR24 pKa = 11.84TISSSSSSSNTITSSSSSSSRR45 pKa = 11.84TITISSSTISTITITTSSSSSSSSTITSSTSSSIIITSSRR85 pKa = 11.84RR86 pKa = 11.84SSSSSTSSSIVTSSTSSSSTNTTTITSSTSTITTTSSRR124 pKa = 11.84SSSTSSSSSSSNSSSSSSSSIPRR147 pKa = 3.42

Molecular weight: 14.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10057

0

10057

4481553

42

7984

445.6

47.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

16.621 ± 0.106

1.648 ± 0.013

3.292 ± 0.022

5.75 ± 0.037

1.977 ± 0.016

5.529 ± 0.035

2.163 ± 0.013

2.207 ± 0.018

3.305 ± 0.031

8.455 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.337 ± 0.01

2.165 ± 0.015

5.404 ± 0.03

14.291 ± 0.123

5.332 ± 0.025

9.696 ± 0.055

4.566 ± 0.021

4.167 ± 0.025

0.803 ± 0.006

1.292 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski