Amino acid dipepetide frequency for Mycobacterium phage SWU2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.938AlaAla: 9.938 ± 1.012
1.154AlaCys: 1.154 ± 0.302
5.899AlaAsp: 5.899 ± 0.705
6.219AlaGlu: 6.219 ± 0.641
3.655AlaPhe: 3.655 ± 0.42
7.566AlaGly: 7.566 ± 0.914
1.346AlaHis: 1.346 ± 0.273
3.975AlaIle: 3.975 ± 0.478
4.039AlaLys: 4.039 ± 0.565
9.04AlaLeu: 9.04 ± 1.035
3.206AlaMet: 3.206 ± 0.433
3.206AlaAsn: 3.206 ± 0.456
4.232AlaPro: 4.232 ± 0.596
3.719AlaGln: 3.719 ± 0.526
4.424AlaArg: 4.424 ± 0.648
5.065AlaSer: 5.065 ± 0.524
5.257AlaThr: 5.257 ± 0.595
7.758AlaVal: 7.758 ± 0.786
1.475AlaTrp: 1.475 ± 0.308
2.949AlaTyr: 2.949 ± 0.539
0.0AlaXaa: 0.0 ± 0.0
Cys
0.833CysAla: 0.833 ± 0.214
0.0CysCys: 0.0 ± 0.0
0.577CysAsp: 0.577 ± 0.197
0.898CysGlu: 0.898 ± 0.263
0.192CysPhe: 0.192 ± 0.119
0.705CysGly: 0.705 ± 0.252
0.192CysHis: 0.192 ± 0.111
0.321CysIle: 0.321 ± 0.148
0.321CysLys: 0.321 ± 0.12
0.833CysLeu: 0.833 ± 0.257
0.256CysMet: 0.256 ± 0.137
0.256CysAsn: 0.256 ± 0.115
0.385CysPro: 0.385 ± 0.155
0.256CysGln: 0.256 ± 0.136
0.641CysArg: 0.641 ± 0.22
0.128CysSer: 0.128 ± 0.078
0.128CysThr: 0.128 ± 0.089
0.641CysVal: 0.641 ± 0.186
0.385CysTrp: 0.385 ± 0.162
0.192CysTyr: 0.192 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
5.963AspAla: 5.963 ± 0.599
0.449AspCys: 0.449 ± 0.169
4.36AspAsp: 4.36 ± 0.604
4.937AspGlu: 4.937 ± 0.509
2.18AspPhe: 2.18 ± 0.44
6.924AspGly: 6.924 ± 0.713
1.603AspHis: 1.603 ± 0.374
2.565AspIle: 2.565 ± 0.472
2.693AspLys: 2.693 ± 0.471
5.642AspLeu: 5.642 ± 0.589
0.898AspMet: 0.898 ± 0.265
1.475AspAsn: 1.475 ± 0.293
4.424AspPro: 4.424 ± 0.612
2.18AspGln: 2.18 ± 0.383
3.975AspArg: 3.975 ± 0.551
3.013AspSer: 3.013 ± 0.418
4.103AspThr: 4.103 ± 0.495
5.45AspVal: 5.45 ± 0.497
1.603AspTrp: 1.603 ± 0.318
2.436AspTyr: 2.436 ± 0.376
0.0AspXaa: 0.0 ± 0.0
Glu
6.155GluAla: 6.155 ± 0.677
0.513GluCys: 0.513 ± 0.149
4.937GluAsp: 4.937 ± 0.566
4.167GluGlu: 4.167 ± 0.611
2.436GluPhe: 2.436 ± 0.342
6.476GluGly: 6.476 ± 1.217
0.577GluHis: 0.577 ± 0.24
3.142GluIle: 3.142 ± 0.414
2.436GluLys: 2.436 ± 0.311
5.706GluLeu: 5.706 ± 0.593
1.667GluMet: 1.667 ± 0.354
1.667GluAsn: 1.667 ± 0.286
2.693GluPro: 2.693 ± 0.507
2.565GluGln: 2.565 ± 0.46
4.745GluArg: 4.745 ± 0.684
2.757GluSer: 2.757 ± 0.395
3.27GluThr: 3.27 ± 0.446
5.193GluVal: 5.193 ± 0.597
1.346GluTrp: 1.346 ± 0.282
1.603GluTyr: 1.603 ± 0.299
0.0GluXaa: 0.0 ± 0.0
Phe
2.565PheAla: 2.565 ± 0.43
0.256PheCys: 0.256 ± 0.128
3.013PheAsp: 3.013 ± 0.42
2.18PheGlu: 2.18 ± 0.41
0.833PhePhe: 0.833 ± 0.223
3.398PheGly: 3.398 ± 0.488
0.513PheHis: 0.513 ± 0.152
1.859PheIle: 1.859 ± 0.348
1.603PheLys: 1.603 ± 0.295
2.693PheLeu: 2.693 ± 0.367
0.577PheMet: 0.577 ± 0.19
1.218PheAsn: 1.218 ± 0.339
2.052PhePro: 2.052 ± 0.37
1.282PheGln: 1.282 ± 0.267
1.859PheArg: 1.859 ± 0.328
2.052PheSer: 2.052 ± 0.331
1.795PheThr: 1.795 ± 0.297
2.629PheVal: 2.629 ± 0.489
0.256PheTrp: 0.256 ± 0.141
0.513PheTyr: 0.513 ± 0.186
0.0PheXaa: 0.0 ± 0.0
Gly
6.604GlyAla: 6.604 ± 0.924
0.962GlyCys: 0.962 ± 0.305
5.899GlyAsp: 5.899 ± 0.599
5.257GlyGlu: 5.257 ± 0.465
3.334GlyPhe: 3.334 ± 0.544
8.784GlyGly: 8.784 ± 2.234
2.308GlyHis: 2.308 ± 0.37
4.745GlyIle: 4.745 ± 0.471
3.719GlyLys: 3.719 ± 0.426
6.732GlyLeu: 6.732 ± 0.847
2.244GlyMet: 2.244 ± 0.343
2.821GlyAsn: 2.821 ± 0.47
7.245GlyPro: 7.245 ± 2.368
2.565GlyGln: 2.565 ± 0.385
4.296GlyArg: 4.296 ± 0.526
5.386GlySer: 5.386 ± 0.654
6.668GlyThr: 6.668 ± 0.975
6.796GlyVal: 6.796 ± 0.929
2.18GlyTrp: 2.18 ± 0.331
2.949GlyTyr: 2.949 ± 0.523
0.0GlyXaa: 0.0 ± 0.0
His
1.475HisAla: 1.475 ± 0.322
0.256HisCys: 0.256 ± 0.129
1.539HisAsp: 1.539 ± 0.324
1.346HisGlu: 1.346 ± 0.316
0.962HisPhe: 0.962 ± 0.262
1.795HisGly: 1.795 ± 0.38
0.321HisHis: 0.321 ± 0.125
0.577HisIle: 0.577 ± 0.184
0.705HisLys: 0.705 ± 0.191
1.411HisLeu: 1.411 ± 0.298
0.192HisMet: 0.192 ± 0.119
0.192HisAsn: 0.192 ± 0.108
1.475HisPro: 1.475 ± 0.329
0.577HisGln: 0.577 ± 0.209
1.795HisArg: 1.795 ± 0.305
0.769HisSer: 0.769 ± 0.21
1.539HisThr: 1.539 ± 0.324
1.539HisVal: 1.539 ± 0.229
0.449HisTrp: 0.449 ± 0.189
0.256HisTyr: 0.256 ± 0.153
0.0HisXaa: 0.0 ± 0.0
Ile
5.257IleAla: 5.257 ± 0.478
0.256IleCys: 0.256 ± 0.122
3.847IleAsp: 3.847 ± 0.442
3.59IleGlu: 3.59 ± 0.58
1.346IlePhe: 1.346 ± 0.3
3.398IleGly: 3.398 ± 0.475
1.282IleHis: 1.282 ± 0.292
1.795IleIle: 1.795 ± 0.351
1.859IleLys: 1.859 ± 0.373
3.27IleLeu: 3.27 ± 0.379
0.321IleMet: 0.321 ± 0.115
2.18IleAsn: 2.18 ± 0.398
4.36IlePro: 4.36 ± 0.515
1.539IleGln: 1.539 ± 0.435
3.911IleArg: 3.911 ± 0.46
2.436IleSer: 2.436 ± 0.519
2.885IleThr: 2.885 ± 0.373
2.372IleVal: 2.372 ± 0.374
0.705IleTrp: 0.705 ± 0.211
0.769IleTyr: 0.769 ± 0.186
0.0IleXaa: 0.0 ± 0.0
Lys
4.167LysAla: 4.167 ± 0.456
0.321LysCys: 0.321 ± 0.14
3.013LysAsp: 3.013 ± 0.43
1.923LysGlu: 1.923 ± 0.38
1.346LysPhe: 1.346 ± 0.291
4.103LysGly: 4.103 ± 0.448
0.898LysHis: 0.898 ± 0.274
2.18LysIle: 2.18 ± 0.435
2.052LysLys: 2.052 ± 0.38
3.975LysLeu: 3.975 ± 0.478
0.833LysMet: 0.833 ± 0.267
1.218LysAsn: 1.218 ± 0.262
3.142LysPro: 3.142 ± 0.509
1.346LysGln: 1.346 ± 0.239
2.5LysArg: 2.5 ± 0.38
1.859LysSer: 1.859 ± 0.262
2.116LysThr: 2.116 ± 0.378
3.783LysVal: 3.783 ± 0.551
0.705LysTrp: 0.705 ± 0.187
1.09LysTyr: 1.09 ± 0.261
0.0LysXaa: 0.0 ± 0.0
Leu
8.143LeuAla: 8.143 ± 1.126
0.577LeuCys: 0.577 ± 0.202
4.937LeuAsp: 4.937 ± 0.5
4.488LeuGlu: 4.488 ± 0.478
2.052LeuPhe: 2.052 ± 0.335
7.886LeuGly: 7.886 ± 0.669
1.795LeuHis: 1.795 ± 0.441
5.001LeuIle: 5.001 ± 0.409
3.27LeuLys: 3.27 ± 0.391
5.706LeuLeu: 5.706 ± 0.639
2.629LeuMet: 2.629 ± 0.382
3.142LeuAsn: 3.142 ± 0.506
4.103LeuPro: 4.103 ± 0.502
3.526LeuGln: 3.526 ± 0.573
6.219LeuArg: 6.219 ± 0.631
4.873LeuSer: 4.873 ± 0.521
5.834LeuThr: 5.834 ± 0.923
5.129LeuVal: 5.129 ± 0.603
1.411LeuTrp: 1.411 ± 0.33
2.372LeuTyr: 2.372 ± 0.509
0.0LeuXaa: 0.0 ± 0.0
Met
2.565MetAla: 2.565 ± 0.443
0.0MetCys: 0.0 ± 0.0
1.218MetAsp: 1.218 ± 0.249
1.411MetGlu: 1.411 ± 0.276
0.577MetPhe: 0.577 ± 0.162
1.859MetGly: 1.859 ± 0.417
0.256MetHis: 0.256 ± 0.13
0.898MetIle: 0.898 ± 0.248
1.09MetLys: 1.09 ± 0.241
2.052MetLeu: 2.052 ± 0.29
0.513MetMet: 0.513 ± 0.164
1.026MetAsn: 1.026 ± 0.224
1.603MetPro: 1.603 ± 0.306
0.833MetGln: 0.833 ± 0.227
1.603MetArg: 1.603 ± 0.281
1.795MetSer: 1.795 ± 0.303
2.565MetThr: 2.565 ± 0.322
1.154MetVal: 1.154 ± 0.205
0.192MetTrp: 0.192 ± 0.138
0.705MetTyr: 0.705 ± 0.175
0.0MetXaa: 0.0 ± 0.0
Asn
3.078AsnAla: 3.078 ± 0.371
0.128AsnCys: 0.128 ± 0.082
2.18AsnAsp: 2.18 ± 0.356
1.603AsnGlu: 1.603 ± 0.33
1.218AsnPhe: 1.218 ± 0.285
3.013AsnGly: 3.013 ± 0.47
0.833AsnHis: 0.833 ± 0.222
1.475AsnIle: 1.475 ± 0.325
1.539AsnLys: 1.539 ± 0.281
2.565AsnLeu: 2.565 ± 0.473
0.705AsnMet: 0.705 ± 0.216
0.577AsnAsn: 0.577 ± 0.18
2.308AsnPro: 2.308 ± 0.375
1.026AsnGln: 1.026 ± 0.218
1.859AsnArg: 1.859 ± 0.351
1.731AsnSer: 1.731 ± 0.343
2.052AsnThr: 2.052 ± 0.483
2.308AsnVal: 2.308 ± 0.366
0.769AsnTrp: 0.769 ± 0.162
0.833AsnTyr: 0.833 ± 0.183
0.0AsnXaa: 0.0 ± 0.0
Pro
4.424ProAla: 4.424 ± 0.518
0.321ProCys: 0.321 ± 0.138
3.975ProAsp: 3.975 ± 0.474
5.706ProGlu: 5.706 ± 1.1
1.411ProPhe: 1.411 ± 0.356
5.514ProGly: 5.514 ± 0.568
0.962ProHis: 0.962 ± 0.208
2.5ProIle: 2.5 ± 0.311
2.18ProLys: 2.18 ± 0.397
4.809ProLeu: 4.809 ± 0.557
0.962ProMet: 0.962 ± 0.238
1.731ProAsn: 1.731 ± 0.315
3.013ProPro: 3.013 ± 0.533
3.27ProGln: 3.27 ± 0.828
2.757ProArg: 2.757 ± 0.44
2.949ProSer: 2.949 ± 0.388
3.719ProThr: 3.719 ± 0.625
4.745ProVal: 4.745 ± 0.605
1.218ProTrp: 1.218 ± 0.506
1.667ProTyr: 1.667 ± 0.305
0.0ProXaa: 0.0 ± 0.0
Gln
4.68GlnAla: 4.68 ± 0.613
0.192GlnCys: 0.192 ± 0.106
1.603GlnAsp: 1.603 ± 0.361
2.308GlnGlu: 2.308 ± 0.485
1.218GlnPhe: 1.218 ± 0.349
3.847GlnGly: 3.847 ± 1.107
0.641GlnHis: 0.641 ± 0.19
2.5GlnIle: 2.5 ± 0.524
1.539GlnLys: 1.539 ± 0.282
3.27GlnLeu: 3.27 ± 0.642
1.282GlnMet: 1.282 ± 0.331
1.09GlnAsn: 1.09 ± 0.31
1.603GlnPro: 1.603 ± 0.322
1.667GlnGln: 1.667 ± 0.381
2.821GlnArg: 2.821 ± 0.413
1.603GlnSer: 1.603 ± 0.421
2.116GlnThr: 2.116 ± 0.437
2.885GlnVal: 2.885 ± 0.522
0.769GlnTrp: 0.769 ± 0.226
0.962GlnTyr: 0.962 ± 0.218
0.0GlnXaa: 0.0 ± 0.0
Arg
6.027ArgAla: 6.027 ± 0.707
0.962ArgCys: 0.962 ± 0.296
3.526ArgAsp: 3.526 ± 0.547
4.36ArgGlu: 4.36 ± 0.62
2.052ArgPhe: 2.052 ± 0.333
4.039ArgGly: 4.039 ± 0.525
1.346ArgHis: 1.346 ± 0.291
3.142ArgIle: 3.142 ± 0.361
3.206ArgLys: 3.206 ± 0.518
5.578ArgLeu: 5.578 ± 0.741
2.052ArgMet: 2.052 ± 0.385
1.923ArgAsn: 1.923 ± 0.284
2.18ArgPro: 2.18 ± 0.418
3.27ArgGln: 3.27 ± 0.542
5.129ArgArg: 5.129 ± 0.595
2.629ArgSer: 2.629 ± 0.362
3.013ArgThr: 3.013 ± 0.379
4.424ArgVal: 4.424 ± 0.551
1.282ArgTrp: 1.282 ± 0.352
1.923ArgTyr: 1.923 ± 0.387
0.0ArgXaa: 0.0 ± 0.0
Ser
4.68SerAla: 4.68 ± 0.71
0.321SerCys: 0.321 ± 0.138
4.167SerAsp: 4.167 ± 0.542
2.757SerGlu: 2.757 ± 0.355
2.18SerPhe: 2.18 ± 0.369
5.642SerGly: 5.642 ± 0.677
1.218SerHis: 1.218 ± 0.307
2.18SerIle: 2.18 ± 0.314
2.052SerLys: 2.052 ± 0.432
4.937SerLeu: 4.937 ± 0.62
1.731SerMet: 1.731 ± 0.39
1.667SerAsn: 1.667 ± 0.305
2.5SerPro: 2.5 ± 0.377
2.372SerGln: 2.372 ± 0.447
3.59SerArg: 3.59 ± 0.558
2.885SerSer: 2.885 ± 0.466
2.885SerThr: 2.885 ± 0.479
3.078SerVal: 3.078 ± 0.448
1.026SerTrp: 1.026 ± 0.246
1.218SerTyr: 1.218 ± 0.286
0.0SerXaa: 0.0 ± 0.0
Thr
6.347ThrAla: 6.347 ± 0.743
0.321ThrCys: 0.321 ± 0.137
3.847ThrAsp: 3.847 ± 0.499
3.59ThrGlu: 3.59 ± 0.478
2.372ThrPhe: 2.372 ± 0.528
7.053ThrGly: 7.053 ± 0.709
0.898ThrHis: 0.898 ± 0.222
2.308ThrIle: 2.308 ± 0.42
2.885ThrLys: 2.885 ± 0.372
5.77ThrLeu: 5.77 ± 0.747
1.346ThrMet: 1.346 ± 0.283
1.603ThrAsn: 1.603 ± 0.304
3.783ThrPro: 3.783 ± 0.494
1.859ThrGln: 1.859 ± 0.449
2.821ThrArg: 2.821 ± 0.351
3.206ThrSer: 3.206 ± 0.554
4.745ThrThr: 4.745 ± 0.646
4.552ThrVal: 4.552 ± 0.635
0.962ThrTrp: 0.962 ± 0.228
1.603ThrTyr: 1.603 ± 0.363
0.0ThrXaa: 0.0 ± 0.0
Val
6.989ValAla: 6.989 ± 0.684
0.705ValCys: 0.705 ± 0.243
4.68ValAsp: 4.68 ± 0.572
5.001ValGlu: 5.001 ± 0.485
2.372ValPhe: 2.372 ± 0.385
5.706ValGly: 5.706 ± 0.718
1.282ValHis: 1.282 ± 0.299
3.783ValIle: 3.783 ± 0.449
3.655ValLys: 3.655 ± 0.467
5.193ValLeu: 5.193 ± 0.538
1.09ValMet: 1.09 ± 0.258
2.693ValAsn: 2.693 ± 0.427
4.167ValPro: 4.167 ± 0.573
2.757ValGln: 2.757 ± 0.497
4.424ValArg: 4.424 ± 0.598
5.065ValSer: 5.065 ± 0.525
4.488ValThr: 4.488 ± 0.558
6.924ValVal: 6.924 ± 0.629
1.09ValTrp: 1.09 ± 0.272
2.372ValTyr: 2.372 ± 0.454
0.0ValXaa: 0.0 ± 0.0
Trp
1.988TrpAla: 1.988 ± 0.5
0.192TrpCys: 0.192 ± 0.114
1.346TrpAsp: 1.346 ± 0.302
0.898TrpGlu: 0.898 ± 0.227
0.513TrpPhe: 0.513 ± 0.181
1.731TrpGly: 1.731 ± 0.35
0.385TrpHis: 0.385 ± 0.144
1.026TrpIle: 1.026 ± 0.212
0.577TrpLys: 0.577 ± 0.199
1.346TrpLeu: 1.346 ± 0.298
0.641TrpMet: 0.641 ± 0.204
1.026TrpAsn: 1.026 ± 0.288
0.833TrpPro: 0.833 ± 0.249
0.769TrpGln: 0.769 ± 0.206
0.962TrpArg: 0.962 ± 0.23
1.346TrpSer: 1.346 ± 0.301
1.026TrpThr: 1.026 ± 0.276
1.09TrpVal: 1.09 ± 0.206
0.513TrpTrp: 0.513 ± 0.231
0.513TrpTyr: 0.513 ± 0.161
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.244TyrAla: 2.244 ± 0.431
0.256TyrCys: 0.256 ± 0.131
2.308TyrAsp: 2.308 ± 0.425
1.411TyrGlu: 1.411 ± 0.309
0.898TyrPhe: 0.898 ± 0.252
2.052TyrGly: 2.052 ± 0.421
0.513TyrHis: 0.513 ± 0.173
1.411TyrIle: 1.411 ± 0.288
1.154TyrLys: 1.154 ± 0.323
2.629TyrLeu: 2.629 ± 0.403
0.705TyrMet: 0.705 ± 0.249
1.09TyrAsn: 1.09 ± 0.257
1.731TyrPro: 1.731 ± 0.362
1.218TyrGln: 1.218 ± 0.255
1.731TyrArg: 1.731 ± 0.342
1.539TyrSer: 1.539 ± 0.326
1.667TyrThr: 1.667 ± 0.401
1.988TyrVal: 1.988 ± 0.308
0.385TyrTrp: 0.385 ± 0.134
0.705TyrTyr: 0.705 ± 0.19
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (15598 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski