Saprolegnia parasitica (strain CBS 223.65)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae; Saprolegnia; Saprolegnia parasitica

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20052 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A067BWZ5|A0A067BWZ5_SAPPC Uncharacterized protein OS=Saprolegnia parasitica (strain CBS 223.65) OX=695850 GN=SPRG_12205 PE=4 SV=1
MM1 pKa = 7.09QRR3 pKa = 11.84LVCLTALLAAGAHH16 pKa = 6.53AASQPAWHH24 pKa = 6.3VCHH27 pKa = 6.44RR28 pKa = 11.84QHH30 pKa = 7.42DD31 pKa = 3.91ACATKK36 pKa = 10.65NWVCCVGPDD45 pKa = 5.08DD46 pKa = 3.83ITSGKK51 pKa = 5.0TTCRR55 pKa = 11.84PTCEE59 pKa = 4.02TTPPRR64 pKa = 11.84MKK66 pKa = 10.06LTEE69 pKa = 4.7DD70 pKa = 4.77CDD72 pKa = 5.99DD73 pKa = 6.15DD74 pKa = 7.48DD75 pKa = 7.57DD76 pKa = 7.41DD77 pKa = 6.35DD78 pKa = 6.14GNYY81 pKa = 10.7DD82 pKa = 5.56DD83 pKa = 6.7DD84 pKa = 7.41DD85 pKa = 7.12GDD87 pKa = 5.36DD88 pKa = 4.6GDD90 pKa = 5.08DD91 pKa = 3.79SGSGSVDD98 pKa = 3.3PSDD101 pKa = 5.79DD102 pKa = 3.76EE103 pKa = 5.42PSDD106 pKa = 4.2EE107 pKa = 4.96PCDD110 pKa = 4.83DD111 pKa = 4.88DD112 pKa = 6.35PSDD115 pKa = 4.08EE116 pKa = 4.83PSDD119 pKa = 3.97EE120 pKa = 4.66PSDD123 pKa = 3.99EE124 pKa = 4.66PSDD127 pKa = 3.99EE128 pKa = 4.66PSDD131 pKa = 3.99EE132 pKa = 4.66PSDD135 pKa = 3.99EE136 pKa = 4.66PSDD139 pKa = 3.99EE140 pKa = 4.66PSDD143 pKa = 3.96EE144 pKa = 4.73PSDD147 pKa = 3.99KK148 pKa = 11.03PSDD151 pKa = 3.8EE152 pKa = 4.74PSDD155 pKa = 3.8SGSGSAYY162 pKa = 8.4PTDD165 pKa = 3.9EE166 pKa = 4.92PSDD169 pKa = 4.03EE170 pKa = 4.68PSDD173 pKa = 4.12APSDD177 pKa = 4.06YY178 pKa = 10.5PSQDD182 pKa = 2.66PVYY185 pKa = 8.51PTEE188 pKa = 5.04DD189 pKa = 4.03PSPSPSPSNKK199 pKa = 7.68PTLGPRR205 pKa = 11.84PVTGDD210 pKa = 3.46LKK212 pKa = 10.19TQIIYY217 pKa = 7.06QTSVIRR223 pKa = 11.84AAHH226 pKa = 5.89GLGPVTWNDD235 pKa = 2.97EE236 pKa = 4.31LGAKK240 pKa = 7.4MQAWADD246 pKa = 3.83SNPQQNGGGHH256 pKa = 7.01GGPPGNQNLASFLVCKK272 pKa = 9.69NDD274 pKa = 3.37CMASAGPAWSWYY286 pKa = 10.12SGEE289 pKa = 4.13EE290 pKa = 4.19KK291 pKa = 10.53LWDD294 pKa = 3.55YY295 pKa = 8.86NTNKK299 pKa = 10.48SKK301 pKa = 10.99DD302 pKa = 4.11GNWMTTGHH310 pKa = 6.99FSNSMDD316 pKa = 3.67PGVNEE321 pKa = 4.05IACGYY326 pKa = 7.96STFYY330 pKa = 11.17NPTIQADD337 pKa = 4.49DD338 pKa = 3.74SLVWCNYY345 pKa = 10.3LGGNDD350 pKa = 3.68KK351 pKa = 10.69PIPRR355 pKa = 11.84PLMDD359 pKa = 4.49QEE361 pKa = 5.14ALMKK365 pKa = 10.59KK366 pKa = 10.0LVSAYY371 pKa = 10.34

Molecular weight: 39.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A067BJ79|A0A067BJ79_SAPPC Uncharacterized protein OS=Saprolegnia parasitica (strain CBS 223.65) OX=695850 GN=SPRG_15383 PE=3 SV=1
MM1 pKa = 7.42APSVMSLSRR10 pKa = 11.84PTWLPAVPPRR20 pKa = 11.84AFVKK24 pKa = 10.15RR25 pKa = 11.84RR26 pKa = 11.84PRR28 pKa = 11.84PRR30 pKa = 11.84TRR32 pKa = 11.84SFAAFTPTVATTLQANTMMANGSGVTSSVGTKK64 pKa = 9.97KK65 pKa = 10.15IKK67 pKa = 10.2QPPHH71 pKa = 5.18PRR73 pKa = 11.84ARR75 pKa = 3.57

Molecular weight: 8.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20052

0

20052

8654731

45

9440

431.6

47.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.854 ± 0.02

1.857 ± 0.011

5.785 ± 0.015

4.959 ± 0.02

3.617 ± 0.012

5.594 ± 0.023

2.947 ± 0.012

3.961 ± 0.012

4.204 ± 0.021

10.567 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.008

2.991 ± 0.011

5.362 ± 0.016

3.684 ± 0.01

5.897 ± 0.02

7.239 ± 0.018

6.432 ± 0.019

7.468 ± 0.016

1.342 ± 0.006

2.769 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski