Wuhan insect virus 20

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGQ5|A0A1L3KGQ5_9VIRU Uncharacterized protein OS=Wuhan insect virus 20 OX=1923724 PE=4 SV=1
MM1 pKa = 7.76FDD3 pKa = 3.37QLLTASVRR11 pKa = 11.84VIRR14 pKa = 11.84DD15 pKa = 4.57FISFCYY21 pKa = 10.01NKK23 pKa = 10.3AGSIYY28 pKa = 10.38CSLKK32 pKa = 9.82KK33 pKa = 9.82WLWDD37 pKa = 3.57LQGKK41 pKa = 7.2FQQYY45 pKa = 10.63DD46 pKa = 3.49AFVDD50 pKa = 4.03LCYY53 pKa = 11.13GFMDD57 pKa = 4.14DD58 pKa = 4.05VEE60 pKa = 4.23EE61 pKa = 5.46AEE63 pKa = 4.24FEE65 pKa = 4.05RR66 pKa = 11.84LQVYY70 pKa = 9.72EE71 pKa = 4.4DD72 pKa = 3.67SLMEE76 pKa = 3.82EE77 pKa = 3.82SLARR81 pKa = 11.84KK82 pKa = 8.61EE83 pKa = 4.0LSRR86 pKa = 11.84ILALPVAGCWPMPTRR101 pKa = 11.84PPGSVNCEE109 pKa = 3.6EE110 pKa = 4.41YY111 pKa = 10.59GYY113 pKa = 11.08EE114 pKa = 4.01SALPADD120 pKa = 4.25HH121 pKa = 6.55QALIPEE127 pKa = 4.43EE128 pKa = 3.87NVQAVKK134 pKa = 10.09PVPLKK139 pKa = 10.38LQPKK143 pKa = 7.86TAVSQEE149 pKa = 3.77EE150 pKa = 4.6RR151 pKa = 11.84EE152 pKa = 4.2TAFLNFAAATAKK164 pKa = 10.07FDD166 pKa = 4.65QIKK169 pKa = 8.39EE170 pKa = 4.46TIKK173 pKa = 10.78DD174 pKa = 3.75LYY176 pKa = 11.06AEE178 pKa = 4.38EE179 pKa = 5.04RR180 pKa = 11.84GPTPFGRR187 pKa = 11.84WFGTLKK193 pKa = 10.34QRR195 pKa = 11.84MATVKK200 pKa = 8.99QAKK203 pKa = 9.07IRR205 pKa = 11.84RR206 pKa = 11.84EE207 pKa = 3.68RR208 pKa = 11.84SKK210 pKa = 11.01VYY212 pKa = 9.7AAKK215 pKa = 10.54VEE217 pKa = 3.9QEE219 pKa = 3.78MAYY222 pKa = 9.91KK223 pKa = 10.55EE224 pKa = 4.35NIPEE228 pKa = 4.17LLALTICVEE237 pKa = 3.96VDD239 pKa = 2.81TGEE242 pKa = 4.25PLPVKK247 pKa = 10.16KK248 pKa = 10.44DD249 pKa = 3.22EE250 pKa = 4.27QGAVRR255 pKa = 11.84AGFSPGTKK263 pKa = 9.16KK264 pKa = 10.89VLMRR268 pKa = 11.84QISSSPSDD276 pKa = 3.53RR277 pKa = 11.84KK278 pKa = 10.49DD279 pKa = 2.92AANWIRR285 pKa = 11.84HH286 pKa = 4.03YY287 pKa = 10.99VRR289 pKa = 11.84NKK291 pKa = 9.44NRR293 pKa = 11.84RR294 pKa = 11.84LSSDD298 pKa = 3.62EE299 pKa = 4.01VSHH302 pKa = 6.27ATIQRR307 pKa = 11.84YY308 pKa = 8.17VEE310 pKa = 4.34QICSDD315 pKa = 4.13LKK317 pKa = 11.23LDD319 pKa = 4.33LSSTTFLLDD328 pKa = 3.24KK329 pKa = 11.05ALMTVPVPTKK339 pKa = 10.35RR340 pKa = 11.84DD341 pKa = 3.43LKK343 pKa = 10.77SAMIVHH349 pKa = 6.39SPAACSLRR357 pKa = 11.84RR358 pKa = 11.84EE359 pKa = 4.1LAVLNSSVFF368 pKa = 3.34

Molecular weight: 41.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGW1|A0A1L3KGW1_9VIRU RNA-directed RNA polymerase OS=Wuhan insect virus 20 OX=1923724 PE=4 SV=1
MM1 pKa = 7.53EE2 pKa = 5.62LANTYY7 pKa = 10.93SSGKK11 pKa = 7.82KK12 pKa = 8.91AQYY15 pKa = 10.17ISAVLNLRR23 pKa = 11.84KK24 pKa = 9.83RR25 pKa = 11.84PVEE28 pKa = 3.9QKK30 pKa = 10.8DD31 pKa = 3.75SYY33 pKa = 9.82VTAFLKK39 pKa = 9.99MEE41 pKa = 4.28KK42 pKa = 9.74HH43 pKa = 5.07WMCKK47 pKa = 9.89KK48 pKa = 9.69IAPRR52 pKa = 11.84LICPRR57 pKa = 11.84TKK59 pKa = 10.26RR60 pKa = 11.84YY61 pKa = 9.61NVEE64 pKa = 3.48LGRR67 pKa = 11.84RR68 pKa = 11.84LKK70 pKa = 10.88FNEE73 pKa = 4.33KK74 pKa = 10.43KK75 pKa = 10.46FMHH78 pKa = 7.14AIDD81 pKa = 4.38AVFGSPTVLSGYY93 pKa = 11.02DD94 pKa = 3.26NFNVGKK100 pKa = 10.1IIKK103 pKa = 9.88KK104 pKa = 9.94KK105 pKa = 7.63WDD107 pKa = 3.51KK108 pKa = 10.95FNEE111 pKa = 4.14PVAIGVDD118 pKa = 3.52ASRR121 pKa = 11.84FDD123 pKa = 3.45QHH125 pKa = 8.23VSEE128 pKa = 4.57QALRR132 pKa = 11.84WEE134 pKa = 3.84HH135 pKa = 6.57SIYY138 pKa = 11.16NKK140 pKa = 9.77VFHH143 pKa = 7.13DD144 pKa = 4.16PEE146 pKa = 5.19LALLLKK152 pKa = 9.58WQLTNYY158 pKa = 9.82VSLFVEE164 pKa = 4.47DD165 pKa = 4.33RR166 pKa = 11.84MLKK169 pKa = 10.42FKK171 pKa = 11.29VKK173 pKa = 8.36GHH175 pKa = 6.19RR176 pKa = 11.84MSGDD180 pKa = 3.0INTSMGNKK188 pKa = 10.21LIMCGMMHH196 pKa = 7.44KK197 pKa = 10.11YY198 pKa = 10.28IKK200 pKa = 10.5DD201 pKa = 3.54LGVKK205 pKa = 10.33AEE207 pKa = 4.27LCNNGDD213 pKa = 3.8DD214 pKa = 4.66CVIICEE220 pKa = 4.29RR221 pKa = 11.84SDD223 pKa = 3.18EE224 pKa = 4.23RR225 pKa = 11.84KK226 pKa = 9.78FDD228 pKa = 4.41GMQDD232 pKa = 2.65WFLNFGFNMVTEE244 pKa = 4.3EE245 pKa = 3.91PVYY248 pKa = 10.38EE249 pKa = 3.99IEE251 pKa = 5.23KK252 pKa = 11.17LEE254 pKa = 4.19FCQSKK259 pKa = 9.55PVSVGGKK266 pKa = 7.91YY267 pKa = 10.32RR268 pKa = 11.84MVRR271 pKa = 11.84RR272 pKa = 11.84PDD274 pKa = 4.06SISKK278 pKa = 10.28DD279 pKa = 3.55SHH281 pKa = 6.97SLLSMKK287 pKa = 10.31SRR289 pKa = 11.84EE290 pKa = 4.11DD291 pKa = 3.3TKK293 pKa = 11.55SFMSATAQCGLVLNSGVPILEE314 pKa = 4.12AFHH317 pKa = 7.12RR318 pKa = 11.84SMYY321 pKa = 10.23RR322 pKa = 11.84GSGYY326 pKa = 10.91KK327 pKa = 10.04KK328 pKa = 10.45ISDD331 pKa = 3.84AYY333 pKa = 8.2LKK335 pKa = 10.68KK336 pKa = 10.49VISYY340 pKa = 7.91GTDD343 pKa = 2.74EE344 pKa = 4.18RR345 pKa = 11.84LGTRR349 pKa = 11.84RR350 pKa = 11.84SWNDD354 pKa = 2.98EE355 pKa = 3.72PVTMEE360 pKa = 4.41NRR362 pKa = 11.84LSYY365 pKa = 9.82WKK367 pKa = 10.86AFGIDD372 pKa = 3.78PNTQEE377 pKa = 3.94LVEE380 pKa = 4.77RR381 pKa = 11.84YY382 pKa = 9.26FDD384 pKa = 3.93NLDD387 pKa = 3.17VCIEE391 pKa = 4.04SRR393 pKa = 11.84GVKK396 pKa = 10.11SLTPFLQSIVLNIPQHH412 pKa = 6.02PRR414 pKa = 11.84FCC416 pKa = 5.71

Molecular weight: 48.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1050

266

416

350.0

39.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.476 ± 1.355

1.905 ± 0.38

5.81 ± 0.209

6.667 ± 0.68

4.381 ± 0.325

5.238 ± 0.773

2.095 ± 0.458

4.667 ± 0.322

7.81 ± 0.791

7.714 ± 1.103

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.952 ± 0.554

4.19 ± 0.77

4.476 ± 0.481

3.524 ± 0.522

6.19 ± 0.194

8.762 ± 1.077

4.381 ± 0.525

7.429 ± 0.29

1.524 ± 0.085

3.81 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski