Homalodisca vitripennis reovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Phytoreovirus; unclassified Phytoreovirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C1JAW7|C1JAW7_9REOV Suppressor of RNA-mediated gene silencing OS=Homalodisca vitripennis reovirus OX=411854 PE=3 SV=1
MM1 pKa = 7.63SGQKK5 pKa = 10.2LQDD8 pKa = 3.0GTAIKK13 pKa = 10.13RR14 pKa = 11.84INDD17 pKa = 4.76AIQIFQKK24 pKa = 10.34YY25 pKa = 5.97QTGEE29 pKa = 3.92VSKK32 pKa = 11.13SSSNHH37 pKa = 5.32LNQLRR42 pKa = 11.84NIRR45 pKa = 11.84MSVGLAWPVILKK57 pKa = 10.18YY58 pKa = 10.8CFLHH62 pKa = 6.35VSSHH66 pKa = 6.02CGVVRR71 pKa = 11.84FLTDD75 pKa = 3.59LACTVKK81 pKa = 10.89VGVFPIISSIGDD93 pKa = 3.89FDD95 pKa = 4.79PFDD98 pKa = 4.29DD99 pKa = 4.34VGLIFSKK106 pKa = 10.72SCIALKK112 pKa = 10.51ISDD115 pKa = 4.38SSFLTPPDD123 pKa = 3.4SFASKK128 pKa = 9.12LTLFFSAMSVNARR141 pKa = 11.84VEE143 pKa = 3.71TDD145 pKa = 3.12MLHH148 pKa = 6.41EE149 pKa = 5.05IEE151 pKa = 5.74DD152 pKa = 4.15RR153 pKa = 11.84YY154 pKa = 9.08TYY156 pKa = 8.94RR157 pKa = 11.84TGVIGEE163 pKa = 4.22LLSVYY168 pKa = 10.25MNATLNLDD176 pKa = 3.54DD177 pKa = 4.17WVVNLDD183 pKa = 4.01EE184 pKa = 5.49LSQPTRR190 pKa = 11.84AQMLTLLAQNRR201 pKa = 11.84NMISNEE207 pKa = 2.74IRR209 pKa = 11.84NYY211 pKa = 9.44VNKK214 pKa = 10.4NLDD217 pKa = 3.46HH218 pKa = 6.85PSEE221 pKa = 4.23HH222 pKa = 6.56LAAVAKK228 pKa = 10.2KK229 pKa = 10.27YY230 pKa = 8.8NQEE233 pKa = 3.54WDD235 pKa = 3.03IGQPTPNLLPVTSATEE251 pKa = 3.66IDD253 pKa = 4.2GPEE256 pKa = 4.15SDD258 pKa = 4.71TEE260 pKa = 4.16SDD262 pKa = 3.88SYY264 pKa = 11.5SASTDD269 pKa = 3.01LDD271 pKa = 3.49ASLRR275 pKa = 11.84AVSSFPTRR283 pKa = 11.84DD284 pKa = 3.47DD285 pKa = 4.26EE286 pKa = 5.78IEE288 pKa = 4.11FSLDD292 pKa = 3.4DD293 pKa = 3.9LL294 pKa = 4.86

Molecular weight: 32.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C1JAW9|C1JAW9_9REOV Uncharacterized protein OS=Homalodisca vitripennis reovirus OX=411854 PE=4 SV=1
MM1 pKa = 7.26NSSVSHH7 pKa = 6.37VGTITIAPDD16 pKa = 3.24KK17 pKa = 10.82DD18 pKa = 3.34SEE20 pKa = 4.34QIYY23 pKa = 10.13KK24 pKa = 10.31RR25 pKa = 11.84KK26 pKa = 9.91KK27 pKa = 8.6LQLPATLKK35 pKa = 10.47INYY38 pKa = 9.29IKK40 pKa = 10.28DD41 pKa = 3.01AKK43 pKa = 10.71YY44 pKa = 10.22RR45 pKa = 11.84ICACPSDD52 pKa = 3.77SHH54 pKa = 8.89SIDD57 pKa = 3.78DD58 pKa = 4.55CPLGGLPQSFVAHH71 pKa = 5.6KK72 pKa = 8.13TLWLSSLLHH81 pKa = 6.4SSSTSANSRR90 pKa = 11.84KK91 pKa = 9.76AVLPRR96 pKa = 11.84IKK98 pKa = 10.02QSKK101 pKa = 9.47FRR103 pKa = 11.84LTDD106 pKa = 2.99TSRR109 pKa = 11.84KK110 pKa = 9.3VKK112 pKa = 10.55SKK114 pKa = 11.33SEE116 pKa = 4.03DD117 pKa = 3.13ATKK120 pKa = 10.91LEE122 pKa = 4.24RR123 pKa = 11.84ASEE126 pKa = 4.03KK127 pKa = 10.43QLEE130 pKa = 4.53KK131 pKa = 10.44KK132 pKa = 10.4SKK134 pKa = 9.52SSEE137 pKa = 3.99VQEE140 pKa = 4.19SSEE143 pKa = 4.01EE144 pKa = 4.4SEE146 pKa = 4.86GEE148 pKa = 4.18TSHH151 pKa = 6.89SRR153 pKa = 11.84LKK155 pKa = 10.48RR156 pKa = 11.84HH157 pKa = 5.92QKK159 pKa = 9.68SEE161 pKa = 3.95KK162 pKa = 9.76KK163 pKa = 10.05GRR165 pKa = 11.84KK166 pKa = 8.74HH167 pKa = 6.91DD168 pKa = 4.13SKK170 pKa = 10.92STLSTEE176 pKa = 4.24EE177 pKa = 3.79VTKK180 pKa = 10.77LVAAITTEE188 pKa = 4.0ALSKK192 pKa = 9.79LTRR195 pKa = 11.84LKK197 pKa = 10.92DD198 pKa = 3.29

Molecular weight: 22.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

7927

135

1460

609.8

67.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.812 ± 0.425

1.173 ± 0.182

6.068 ± 0.221

4.642 ± 0.429

4.062 ± 0.176

5.097 ± 0.296

1.791 ± 0.203

7.279 ± 0.305

5.853 ± 0.632

9.07 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.813 ± 0.183

5.639 ± 0.268

4.113 ± 0.243

3.141 ± 0.178

4.188 ± 0.258

9.461 ± 0.525

7.039 ± 0.42

6.825 ± 0.315

0.82 ± 0.127

4.113 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski