Amino acid dipepetide frequency for Ruaniaceae bacterium KH17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.406AlaAla: 17.406 ± 0.25
0.845AlaCys: 0.845 ± 0.032
7.02AlaAsp: 7.02 ± 0.105
8.752AlaGlu: 8.752 ± 0.125
3.677AlaPhe: 3.677 ± 0.072
10.419AlaGly: 10.419 ± 0.127
2.443AlaHis: 2.443 ± 0.06
6.257AlaIle: 6.257 ± 0.103
2.953AlaLys: 2.953 ± 0.078
13.027AlaLeu: 13.027 ± 0.142
2.754AlaMet: 2.754 ± 0.062
2.755AlaAsn: 2.755 ± 0.056
5.699AlaPro: 5.699 ± 0.113
3.837AlaGln: 3.837 ± 0.083
8.163AlaArg: 8.163 ± 0.122
6.52AlaSer: 6.52 ± 0.083
7.198AlaThr: 7.198 ± 0.094
10.567AlaVal: 10.567 ± 0.133
1.757AlaTrp: 1.757 ± 0.052
2.378AlaTyr: 2.378 ± 0.058
0.0AlaXaa: 0.0 ± 0.0
Cys
0.755CysAla: 0.755 ± 0.033
0.059CysCys: 0.059 ± 0.009
0.401CysAsp: 0.401 ± 0.024
0.389CysGlu: 0.389 ± 0.021
0.193CysPhe: 0.193 ± 0.015
0.702CysGly: 0.702 ± 0.033
0.127CysHis: 0.127 ± 0.012
0.243CysIle: 0.243 ± 0.017
0.085CysLys: 0.085 ± 0.011
0.517CysLeu: 0.517 ± 0.025
0.11CysMet: 0.11 ± 0.011
0.138CysAsn: 0.138 ± 0.014
0.35CysPro: 0.35 ± 0.02
0.162CysGln: 0.162 ± 0.015
0.36CysArg: 0.36 ± 0.021
0.39CysSer: 0.39 ± 0.022
0.432CysThr: 0.432 ± 0.03
0.484CysVal: 0.484 ± 0.026
0.074CysTrp: 0.074 ± 0.01
0.137CysTyr: 0.137 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.485AspAla: 7.485 ± 0.118
0.329AspCys: 0.329 ± 0.023
4.043AspAsp: 4.043 ± 0.084
4.394AspGlu: 4.394 ± 0.079
1.826AspPhe: 1.826 ± 0.045
6.201AspGly: 6.201 ± 0.12
1.094AspHis: 1.094 ± 0.037
2.376AspIle: 2.376 ± 0.059
1.021AspLys: 1.021 ± 0.044
5.979AspLeu: 5.979 ± 0.097
0.955AspMet: 0.955 ± 0.037
1.057AspAsn: 1.057 ± 0.039
3.873AspPro: 3.873 ± 0.073
1.505AspGln: 1.505 ± 0.046
3.723AspArg: 3.723 ± 0.073
3.097AspSer: 3.097 ± 0.066
2.705AspThr: 2.705 ± 0.06
5.285AspVal: 5.285 ± 0.084
0.883AspTrp: 0.883 ± 0.035
1.32AspTyr: 1.32 ± 0.044
0.0AspXaa: 0.0 ± 0.0
Glu
7.334GluAla: 7.334 ± 0.128
0.359GluCys: 0.359 ± 0.022
3.25GluAsp: 3.25 ± 0.076
3.694GluGlu: 3.694 ± 0.076
2.148GluPhe: 2.148 ± 0.052
4.776GluGly: 4.776 ± 0.071
1.582GluHis: 1.582 ± 0.041
3.725GluIle: 3.725 ± 0.069
1.672GluLys: 1.672 ± 0.056
7.541GluLeu: 7.541 ± 0.1
1.264GluMet: 1.264 ± 0.041
1.654GluAsn: 1.654 ± 0.05
3.299GluPro: 3.299 ± 0.082
2.251GluGln: 2.251 ± 0.055
5.199GluArg: 5.199 ± 0.09
3.576GluSer: 3.576 ± 0.074
3.473GluThr: 3.473 ± 0.07
5.016GluVal: 5.016 ± 0.095
1.022GluTrp: 1.022 ± 0.032
1.504GluTyr: 1.504 ± 0.042
0.0GluXaa: 0.0 ± 0.0
Phe
4.02PheAla: 4.02 ± 0.065
0.189PheCys: 0.189 ± 0.015
2.218PheAsp: 2.218 ± 0.057
1.989PheGlu: 1.989 ± 0.051
1.144PhePhe: 1.144 ± 0.048
3.189PheGly: 3.189 ± 0.069
0.6PheHis: 0.6 ± 0.027
1.415PheIle: 1.415 ± 0.047
0.618PheLys: 0.618 ± 0.028
2.838PheLeu: 2.838 ± 0.073
0.609PheMet: 0.609 ± 0.028
0.79PheAsn: 0.79 ± 0.032
1.504PhePro: 1.504 ± 0.045
0.861PheGln: 0.861 ± 0.038
1.736PheArg: 1.736 ± 0.046
1.981PheSer: 1.981 ± 0.046
2.11PheThr: 2.11 ± 0.051
2.709PheVal: 2.709 ± 0.064
0.473PheTrp: 0.473 ± 0.026
0.702PheTyr: 0.702 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
9.901GlyAla: 9.901 ± 0.139
0.611GlyCys: 0.611 ± 0.031
4.731GlyAsp: 4.731 ± 0.085
5.514GlyGlu: 5.514 ± 0.088
2.971GlyPhe: 2.971 ± 0.059
7.243GlyGly: 7.243 ± 0.115
1.742GlyHis: 1.742 ± 0.049
4.7GlyIle: 4.7 ± 0.076
2.41GlyLys: 2.41 ± 0.064
8.439GlyLeu: 8.439 ± 0.125
2.138GlyMet: 2.138 ± 0.054
1.995GlyAsn: 1.995 ± 0.054
3.559GlyPro: 3.559 ± 0.068
2.501GlyGln: 2.501 ± 0.051
5.425GlyArg: 5.425 ± 0.084
5.189GlySer: 5.189 ± 0.095
5.657GlyThr: 5.657 ± 0.1
7.714GlyVal: 7.714 ± 0.107
1.54GlyTrp: 1.54 ± 0.052
2.361GlyTyr: 2.361 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
2.282HisAla: 2.282 ± 0.06
0.118HisCys: 0.118 ± 0.011
1.198HisAsp: 1.198 ± 0.04
1.362HisGlu: 1.362 ± 0.039
0.598HisPhe: 0.598 ± 0.027
1.888HisGly: 1.888 ± 0.06
0.568HisHis: 0.568 ± 0.027
0.849HisIle: 0.849 ± 0.031
0.384HisLys: 0.384 ± 0.02
2.005HisLeu: 2.005 ± 0.049
0.371HisMet: 0.371 ± 0.019
0.479HisAsn: 0.479 ± 0.023
1.31HisPro: 1.31 ± 0.049
0.506HisGln: 0.506 ± 0.025
1.401HisArg: 1.401 ± 0.047
1.103HisSer: 1.103 ± 0.04
1.162HisThr: 1.162 ± 0.038
1.74HisVal: 1.74 ± 0.044
0.296HisTrp: 0.296 ± 0.019
0.47HisTyr: 0.47 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
7.142IleAla: 7.142 ± 0.113
0.318IleCys: 0.318 ± 0.021
3.506IleAsp: 3.506 ± 0.076
3.465IleGlu: 3.465 ± 0.069
1.362IlePhe: 1.362 ± 0.05
4.694IleGly: 4.694 ± 0.089
0.882IleHis: 0.882 ± 0.031
2.068IleIle: 2.068 ± 0.061
0.97IleLys: 0.97 ± 0.045
4.335IleLeu: 4.335 ± 0.092
0.843IleMet: 0.843 ± 0.033
1.154IleAsn: 1.154 ± 0.042
2.812IlePro: 2.812 ± 0.055
1.162IleGln: 1.162 ± 0.039
2.709IleArg: 2.709 ± 0.058
2.898IleSer: 2.898 ± 0.059
3.217IleThr: 3.217 ± 0.071
4.841IleVal: 4.841 ± 0.087
0.602IleTrp: 0.602 ± 0.03
0.973IleTyr: 0.973 ± 0.041
0.0IleXaa: 0.0 ± 0.0
Lys
2.739LysAla: 2.739 ± 0.07
0.113LysCys: 0.113 ± 0.012
1.414LysAsp: 1.414 ± 0.049
1.281LysGlu: 1.281 ± 0.046
0.595LysPhe: 0.595 ± 0.029
1.846LysGly: 1.846 ± 0.055
0.467LysHis: 0.467 ± 0.025
1.148LysIle: 1.148 ± 0.039
1.042LysLys: 1.042 ± 0.048
2.315LysLeu: 2.315 ± 0.065
0.588LysMet: 0.588 ± 0.027
0.682LysAsn: 0.682 ± 0.034
1.226LysPro: 1.226 ± 0.044
0.724LysGln: 0.724 ± 0.031
1.804LysArg: 1.804 ± 0.048
1.354LysSer: 1.354 ± 0.044
1.293LysThr: 1.293 ± 0.045
2.007LysVal: 2.007 ± 0.053
0.327LysTrp: 0.327 ± 0.023
0.557LysTyr: 0.557 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
14.056LeuAla: 14.056 ± 0.172
0.56LeuCys: 0.56 ± 0.029
6.368LeuAsp: 6.368 ± 0.103
5.598LeuGlu: 5.598 ± 0.092
2.801LeuPhe: 2.801 ± 0.069
8.593LeuGly: 8.593 ± 0.122
1.866LeuHis: 1.866 ± 0.048
5.064LeuIle: 5.064 ± 0.088
2.156LeuLys: 2.156 ± 0.055
9.686LeuLeu: 9.686 ± 0.152
2.014LeuMet: 2.014 ± 0.052
2.426LeuAsn: 2.426 ± 0.054
5.421LeuPro: 5.421 ± 0.073
2.464LeuGln: 2.464 ± 0.056
6.703LeuArg: 6.703 ± 0.108
6.0LeuSer: 6.0 ± 0.094
6.453LeuThr: 6.453 ± 0.09
8.57LeuVal: 8.57 ± 0.121
1.278LeuTrp: 1.278 ± 0.043
1.799LeuTyr: 1.799 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
2.466MetAla: 2.466 ± 0.058
0.135MetCys: 0.135 ± 0.013
1.006MetAsp: 1.006 ± 0.035
0.981MetGlu: 0.981 ± 0.034
0.607MetPhe: 0.607 ± 0.027
1.636MetGly: 1.636 ± 0.048
0.349MetHis: 0.349 ± 0.021
1.146MetIle: 1.146 ± 0.041
0.682MetLys: 0.682 ± 0.029
2.117MetLeu: 2.117 ± 0.06
0.55MetMet: 0.55 ± 0.032
0.637MetAsn: 0.637 ± 0.032
1.178MetPro: 1.178 ± 0.04
0.559MetGln: 0.559 ± 0.028
1.501MetArg: 1.501 ± 0.039
1.609MetSer: 1.609 ± 0.041
1.72MetThr: 1.72 ± 0.043
1.622MetVal: 1.622 ± 0.05
0.27MetTrp: 0.27 ± 0.017
0.351MetTyr: 0.351 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
2.854AsnAla: 2.854 ± 0.067
0.151AsnCys: 0.151 ± 0.014
1.36AsnAsp: 1.36 ± 0.044
1.499AsnGlu: 1.499 ± 0.043
0.755AsnPhe: 0.755 ± 0.039
2.162AsnGly: 2.162 ± 0.058
0.477AsnHis: 0.477 ± 0.025
1.039AsnIle: 1.039 ± 0.036
0.515AsnLys: 0.515 ± 0.027
2.331AsnLeu: 2.331 ± 0.054
0.512AsnMet: 0.512 ± 0.025
0.579AsnAsn: 0.579 ± 0.028
1.846AsnPro: 1.846 ± 0.043
0.713AsnGln: 0.713 ± 0.032
1.432AsnArg: 1.432 ± 0.049
1.37AsnSer: 1.37 ± 0.047
1.338AsnThr: 1.338 ± 0.039
1.984AsnVal: 1.984 ± 0.056
0.427AsnTrp: 0.427 ± 0.024
0.621AsnTyr: 0.621 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
6.054ProAla: 6.054 ± 0.122
0.245ProCys: 0.245 ± 0.019
3.611ProAsp: 3.611 ± 0.071
4.555ProGlu: 4.555 ± 0.082
1.717ProPhe: 1.717 ± 0.044
4.725ProGly: 4.725 ± 0.099
1.093ProHis: 1.093 ± 0.039
2.451ProIle: 2.451 ± 0.055
1.24ProLys: 1.24 ± 0.037
4.621ProLeu: 4.621 ± 0.085
0.993ProMet: 0.993 ± 0.03
1.329ProAsn: 1.329 ± 0.043
2.186ProPro: 2.186 ± 0.069
1.573ProGln: 1.573 ± 0.043
2.995ProArg: 2.995 ± 0.069
3.22ProSer: 3.22 ± 0.068
3.433ProThr: 3.433 ± 0.075
4.4ProVal: 4.4 ± 0.079
0.879ProTrp: 0.879 ± 0.035
1.171ProTyr: 1.171 ± 0.045
0.0ProXaa: 0.0 ± 0.0
Gln
3.343GlnAla: 3.343 ± 0.072
0.135GlnCys: 0.135 ± 0.013
1.307GlnAsp: 1.307 ± 0.042
1.556GlnGlu: 1.556 ± 0.042
0.973GlnPhe: 0.973 ± 0.04
2.051GlnGly: 2.051 ± 0.049
0.628GlnHis: 0.628 ± 0.025
1.727GlnIle: 1.727 ± 0.05
0.763GlnLys: 0.763 ± 0.035
3.269GlnLeu: 3.269 ± 0.071
0.762GlnMet: 0.762 ± 0.03
0.74GlnAsn: 0.74 ± 0.032
1.495GlnPro: 1.495 ± 0.051
1.227GlnGln: 1.227 ± 0.048
2.448GlnArg: 2.448 ± 0.067
1.528GlnSer: 1.528 ± 0.041
1.399GlnThr: 1.399 ± 0.039
2.42GlnVal: 2.42 ± 0.05
0.507GlnTrp: 0.507 ± 0.025
0.723GlnTyr: 0.723 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
7.96ArgAla: 7.96 ± 0.125
0.35ArgCys: 0.35 ± 0.024
3.764ArgAsp: 3.764 ± 0.082
4.85ArgGlu: 4.85 ± 0.086
2.148ArgPhe: 2.148 ± 0.052
5.009ArgGly: 5.009 ± 0.086
1.489ArgHis: 1.489 ± 0.041
3.469ArgIle: 3.469 ± 0.063
1.643ArgLys: 1.643 ± 0.047
6.333ArgLeu: 6.333 ± 0.119
1.66ArgMet: 1.66 ± 0.048
1.562ArgAsn: 1.562 ± 0.049
3.237ArgPro: 3.237 ± 0.076
2.032ArgGln: 2.032 ± 0.051
5.463ArgArg: 5.463 ± 0.111
3.892ArgSer: 3.892 ± 0.079
4.036ArgThr: 4.036 ± 0.074
5.237ArgVal: 5.237 ± 0.086
1.1ArgTrp: 1.1 ± 0.037
1.604ArgTyr: 1.604 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
6.862SerAla: 6.862 ± 0.1
0.344SerCys: 0.344 ± 0.021
3.231SerAsp: 3.231 ± 0.067
3.577SerGlu: 3.577 ± 0.077
2.064SerPhe: 2.064 ± 0.053
5.798SerGly: 5.798 ± 0.101
1.077SerHis: 1.077 ± 0.039
2.839SerIle: 2.839 ± 0.058
1.357SerLys: 1.357 ± 0.042
5.53SerLeu: 5.53 ± 0.085
1.375SerMet: 1.375 ± 0.042
1.389SerAsn: 1.389 ± 0.04
3.053SerPro: 3.053 ± 0.073
1.755SerGln: 1.755 ± 0.046
3.678SerArg: 3.678 ± 0.077
3.543SerSer: 3.543 ± 0.079
3.476SerThr: 3.476 ± 0.074
4.539SerVal: 4.539 ± 0.08
0.9SerTrp: 0.9 ± 0.036
1.393SerTyr: 1.393 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
7.243ThrAla: 7.243 ± 0.106
0.402ThrCys: 0.402 ± 0.025
3.355ThrAsp: 3.355 ± 0.075
3.65ThrGlu: 3.65 ± 0.071
1.992ThrPhe: 1.992 ± 0.051
5.411ThrGly: 5.411 ± 0.086
1.242ThrHis: 1.242 ± 0.04
3.256ThrIle: 3.256 ± 0.068
1.393ThrLys: 1.393 ± 0.042
6.029ThrLeu: 6.029 ± 0.085
1.206ThrMet: 1.206 ± 0.036
1.41ThrAsn: 1.41 ± 0.041
3.898ThrPro: 3.898 ± 0.075
1.695ThrGln: 1.695 ± 0.045
3.637ThrArg: 3.637 ± 0.071
3.521ThrSer: 3.521 ± 0.067
3.806ThrThr: 3.806 ± 0.077
5.299ThrVal: 5.299 ± 0.103
0.967ThrTrp: 0.967 ± 0.035
1.373ThrTyr: 1.373 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
10.536ValAla: 10.536 ± 0.124
0.549ValCys: 0.549 ± 0.027
5.12ValAsp: 5.12 ± 0.085
5.087ValGlu: 5.087 ± 0.071
2.716ValPhe: 2.716 ± 0.06
6.946ValGly: 6.946 ± 0.083
1.678ValHis: 1.678 ± 0.046
4.616ValIle: 4.616 ± 0.074
1.85ValLys: 1.85 ± 0.063
8.839ValLeu: 8.839 ± 0.13
1.681ValMet: 1.681 ± 0.052
2.137ValAsn: 2.137 ± 0.058
4.687ValPro: 4.687 ± 0.08
2.133ValGln: 2.133 ± 0.055
5.635ValArg: 5.635 ± 0.092
4.85ValSer: 4.85 ± 0.086
5.74ValThr: 5.74 ± 0.102
7.947ValVal: 7.947 ± 0.126
1.101ValTrp: 1.101 ± 0.036
1.596ValTyr: 1.596 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
1.542TrpAla: 1.542 ± 0.043
0.106TrpCys: 0.106 ± 0.012
0.934TrpAsp: 0.934 ± 0.032
0.794TrpGlu: 0.794 ± 0.028
0.595TrpPhe: 0.595 ± 0.027
1.106TrpGly: 1.106 ± 0.045
0.309TrpHis: 0.309 ± 0.022
0.732TrpIle: 0.732 ± 0.037
0.346TrpLys: 0.346 ± 0.02
1.745TrpLeu: 1.745 ± 0.056
0.36TrpMet: 0.36 ± 0.023
0.504TrpAsn: 0.504 ± 0.024
0.716TrpPro: 0.716 ± 0.029
0.592TrpGln: 0.592 ± 0.028
1.148TrpArg: 1.148 ± 0.035
0.899TrpSer: 0.899 ± 0.033
0.866TrpThr: 0.866 ± 0.041
1.143TrpVal: 1.143 ± 0.038
0.384TrpTrp: 0.384 ± 0.024
0.334TrpTyr: 0.334 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.448TyrAla: 2.448 ± 0.058
0.188TyrCys: 0.188 ± 0.015
1.312TyrAsp: 1.312 ± 0.039
1.35TyrGlu: 1.35 ± 0.043
0.813TyrPhe: 0.813 ± 0.033
1.944TyrGly: 1.944 ± 0.055
0.379TyrHis: 0.379 ± 0.024
0.834TyrIle: 0.834 ± 0.034
0.422TyrLys: 0.422 ± 0.021
2.383TyrLeu: 2.383 ± 0.066
0.378TyrMet: 0.378 ± 0.024
0.548TyrAsn: 0.548 ± 0.026
1.144TyrPro: 1.144 ± 0.037
0.75TyrGln: 0.75 ± 0.038
1.678TyrArg: 1.678 ± 0.051
1.24TyrSer: 1.24 ± 0.04
1.273TyrThr: 1.273 ± 0.046
1.918TyrVal: 1.918 ± 0.05
0.363TyrTrp: 0.363 ± 0.023
0.545TyrTyr: 0.545 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2522 proteins (819933 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski