Hubei permutotetra-like virus 10

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KHZ9|A0A1L3KHZ9_9VIRU RdRp OS=Hubei permutotetra-like virus 10 OX=1923074 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 6.1NIMLQRR8 pKa = 11.84QDD10 pKa = 3.47YY11 pKa = 10.86NRR13 pKa = 11.84LQKK16 pKa = 10.46IIATAPRR23 pKa = 11.84TPAPFKK29 pKa = 11.2NPVEE33 pKa = 4.08LEE35 pKa = 4.1IEE37 pKa = 4.08EE38 pKa = 4.48STLKK42 pKa = 10.79RR43 pKa = 11.84LTRR46 pKa = 11.84DD47 pKa = 2.85IPVQGTLDD55 pKa = 3.52LSFLVKK61 pKa = 10.51GSEE64 pKa = 4.24VPLSPEE70 pKa = 3.7HH71 pKa = 6.51TPGLNQKK78 pKa = 9.75GAFVSVNPVFKK89 pKa = 10.05PASRR93 pKa = 11.84DD94 pKa = 3.37RR95 pKa = 11.84KK96 pKa = 9.88VVGAVLRR103 pKa = 11.84RR104 pKa = 11.84GGRR107 pKa = 11.84VEE109 pKa = 5.65DD110 pKa = 3.82IEE112 pKa = 4.25KK113 pKa = 10.68LIYY116 pKa = 7.88TTGSKK121 pKa = 10.3SGFSEE126 pKa = 4.82RR127 pKa = 11.84LKK129 pKa = 10.88LWATARR135 pKa = 11.84AQNPEE140 pKa = 4.08TEE142 pKa = 4.21LKK144 pKa = 10.57EE145 pKa = 4.04RR146 pKa = 11.84LLNAKK151 pKa = 10.12DD152 pKa = 3.84IIRR155 pKa = 11.84KK156 pKa = 8.97RR157 pKa = 11.84LPVRR161 pKa = 11.84EE162 pKa = 4.15KK163 pKa = 11.03LLPDD167 pKa = 3.62WNATVEE173 pKa = 4.16EE174 pKa = 4.76FVAGVTVNKK183 pKa = 10.0SSSAGPPLYY192 pKa = 10.01RR193 pKa = 11.84PKK195 pKa = 10.52YY196 pKa = 8.31QCTDD200 pKa = 2.83EE201 pKa = 4.32YY202 pKa = 10.64MDD204 pKa = 5.05IISEE208 pKa = 4.04IMKK211 pKa = 10.28EE212 pKa = 4.23GNQDD216 pKa = 3.06HH217 pKa = 5.88LTEE220 pKa = 4.08YY221 pKa = 11.18LEE223 pKa = 4.21GRR225 pKa = 11.84EE226 pKa = 4.01EE227 pKa = 5.41FMIAQCKK234 pKa = 9.1NKK236 pKa = 9.4QDD238 pKa = 3.78RR239 pKa = 11.84YY240 pKa = 10.4PPDD243 pKa = 3.63KK244 pKa = 10.67LDD246 pKa = 3.54EE247 pKa = 4.1KK248 pKa = 10.08TRR250 pKa = 11.84PYY252 pKa = 11.16FNFCFPLQFYY262 pKa = 9.68FSALVQPKK270 pKa = 6.9THH272 pKa = 6.65CSVLFTEE279 pKa = 6.47SEE281 pKa = 4.45DD282 pKa = 3.12SWNAYY287 pKa = 8.18GFSWANGGGEE297 pKa = 5.47KK298 pKa = 10.13FFKK301 pKa = 9.73WMKK304 pKa = 9.0SCRR307 pKa = 11.84EE308 pKa = 4.1KK309 pKa = 10.66EE310 pKa = 4.25VKK312 pKa = 10.26LAVYY316 pKa = 10.32GDD318 pKa = 4.05DD319 pKa = 3.39VFLVKK324 pKa = 10.51RR325 pKa = 11.84EE326 pKa = 3.91GGLLKK331 pKa = 10.68ACAPDD336 pKa = 4.06FRR338 pKa = 11.84SMDD341 pKa = 3.31GSVHH345 pKa = 6.67KK346 pKa = 10.64KK347 pKa = 9.38DD348 pKa = 5.06AEE350 pKa = 4.3WFCEE354 pKa = 3.74DD355 pKa = 4.65LIEE358 pKa = 4.85AFEE361 pKa = 4.05RR362 pKa = 11.84QYY364 pKa = 11.73GKK366 pKa = 10.7NNFWSYY372 pKa = 10.69FGKK375 pKa = 10.44LLSRR379 pKa = 11.84SLVGAKK385 pKa = 9.99FLVDD389 pKa = 4.12GPQAYY394 pKa = 10.48VNEE397 pKa = 4.4TGLMTGCVGTTGVDD411 pKa = 3.42TQKK414 pKa = 11.25SVVAYY419 pKa = 10.37ACLLDD424 pKa = 3.69KK425 pKa = 11.37AEE427 pKa = 4.13QKK429 pKa = 10.87EE430 pKa = 3.83IDD432 pKa = 4.32LEE434 pKa = 4.55DD435 pKa = 4.56GEE437 pKa = 5.19AVSTYY442 pKa = 9.72FRR444 pKa = 11.84QKK446 pKa = 9.87FGLIVKK452 pKa = 9.42EE453 pKa = 4.18NTYY456 pKa = 7.71EE457 pKa = 3.94WKK459 pKa = 10.56VVEE462 pKa = 4.38EE463 pKa = 3.99EE464 pKa = 4.33LEE466 pKa = 4.18EE467 pKa = 5.19GEE469 pKa = 4.6VPVDD473 pKa = 4.43LEE475 pKa = 4.06FLGVKK480 pKa = 10.22LKK482 pKa = 9.99MRR484 pKa = 11.84LGKK487 pKa = 9.99EE488 pKa = 3.63RR489 pKa = 11.84LEE491 pKa = 3.9CVPFKK496 pKa = 11.0EE497 pKa = 4.4EE498 pKa = 3.84NDD500 pKa = 3.81LVSLISNARR509 pKa = 11.84IPPEE513 pKa = 3.91AKK515 pKa = 10.02GRR517 pKa = 11.84ATIKK521 pKa = 10.51DD522 pKa = 3.28RR523 pKa = 11.84YY524 pKa = 8.96LFDD527 pKa = 3.25MARR530 pKa = 11.84GYY532 pKa = 10.12MITGAFHH539 pKa = 7.19SEE541 pKa = 4.4VVWNICCDD549 pKa = 4.3LIEE552 pKa = 4.39GTKK555 pKa = 9.88TEE557 pKa = 4.24IVCQRR562 pKa = 11.84VQSGKK567 pKa = 10.07KK568 pKa = 9.72VEE570 pKa = 4.12VAPGKK575 pKa = 9.45WEE577 pKa = 3.9HH578 pKa = 6.36TGQSPEE584 pKa = 4.14LEE586 pKa = 4.06QLVGSDD592 pKa = 4.07FQWPTSDD599 pKa = 3.05GWPSQEE605 pKa = 3.84FCLDD609 pKa = 3.53VYY611 pKa = 10.93LSPDD615 pKa = 3.25NKK617 pKa = 10.32IGGEE621 pKa = 4.11WYY623 pKa = 10.13SCVPSLEE630 pKa = 4.1EE631 pKa = 4.17DD632 pKa = 3.58LKK634 pKa = 11.07KK635 pKa = 10.71LRR637 pKa = 11.84SKK639 pKa = 10.61RR640 pKa = 11.84VTMEE644 pKa = 3.55AAKK647 pKa = 10.57LKK649 pKa = 9.75EE650 pKa = 4.05QGPKK654 pKa = 9.92FDD656 pKa = 3.94WALDD660 pKa = 3.46TDD662 pKa = 4.41LEE664 pKa = 4.52EE665 pKa = 5.04KK666 pKa = 10.59ASQLEE671 pKa = 3.91AVRR674 pKa = 11.84DD675 pKa = 3.98SKK677 pKa = 10.97IDD679 pKa = 3.4PRR681 pKa = 11.84VDD683 pKa = 3.1RR684 pKa = 11.84EE685 pKa = 4.3GYY687 pKa = 10.49LRR689 pKa = 11.84DD690 pKa = 3.62LVKK693 pKa = 10.42EE694 pKa = 4.15RR695 pKa = 11.84PLMRR699 pKa = 11.84PAKK702 pKa = 9.59QPKK705 pKa = 9.62NFLRR709 pKa = 11.84LKK711 pKa = 9.05GQEE714 pKa = 4.15SRR716 pKa = 11.84SKK718 pKa = 10.4DD719 pKa = 3.4QQIGDD724 pKa = 3.98FLEE727 pKa = 4.53KK728 pKa = 10.33FSSVLEE734 pKa = 4.03EE735 pKa = 5.44LPDD738 pKa = 3.48QMLEE742 pKa = 4.22LVVDD746 pKa = 4.55SPPGYY751 pKa = 9.85YY752 pKa = 10.02SRR754 pKa = 11.84VMQGKK759 pKa = 8.61GWRR762 pKa = 11.84PNPDD766 pKa = 2.6RR767 pKa = 11.84KK768 pKa = 9.69VWEE771 pKa = 4.5RR772 pKa = 11.84GSPVPVNPHH781 pKa = 3.23VWGRR785 pKa = 11.84DD786 pKa = 3.01ILMTLPDD793 pKa = 5.62FIPTEE798 pKa = 3.88KK799 pKa = 9.69KK800 pKa = 8.5TVRR803 pKa = 11.84KK804 pKa = 9.45EE805 pKa = 4.04CEE807 pKa = 3.61EE808 pKa = 4.4DD809 pKa = 3.81KK810 pKa = 10.05PTEE813 pKa = 4.19PFTLVPEE820 pKa = 4.3YY821 pKa = 11.19VKK823 pKa = 10.88LLPNVKK829 pKa = 8.89MDD831 pKa = 5.33AISLVTGSFSKK842 pKa = 10.99AGDD845 pKa = 3.16ILTTSNEE852 pKa = 4.11VLRR855 pKa = 11.84LSPDD859 pKa = 2.66SWIKK863 pKa = 9.66TEE865 pKa = 3.7VRR867 pKa = 11.84VRR869 pKa = 11.84NGTGIVARR877 pKa = 11.84AYY879 pKa = 10.31AKK881 pKa = 10.21SGKK884 pKa = 9.11AAKK887 pKa = 9.66EE888 pKa = 3.72MCFEE892 pKa = 4.56NMLEE896 pKa = 3.91QLKK899 pKa = 10.16EE900 pKa = 4.14RR901 pKa = 11.84EE902 pKa = 4.03RR903 pKa = 11.84QPTSFKK909 pKa = 10.78QQTEE913 pKa = 3.86EE914 pKa = 3.97DD915 pKa = 4.12GNRR918 pKa = 11.84STEE921 pKa = 4.0SEE923 pKa = 4.13AAAEE927 pKa = 4.28TQTTEE932 pKa = 4.03RR933 pKa = 11.84EE934 pKa = 4.19EE935 pKa = 4.28EE936 pKa = 3.95NGEE939 pKa = 4.16EE940 pKa = 4.19LVQDD944 pKa = 3.5VCEE947 pKa = 4.17VRR949 pKa = 11.84EE950 pKa = 4.32GTGGGDD956 pKa = 3.45GGVHH960 pKa = 6.6LL961 pKa = 5.16

Molecular weight: 109.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KHZ9|A0A1L3KHZ9_9VIRU RdRp OS=Hubei permutotetra-like virus 10 OX=1923074 PE=4 SV=1
MM1 pKa = 7.02ATEE4 pKa = 3.82AQKK7 pKa = 11.13AKK9 pKa = 10.45RR10 pKa = 11.84RR11 pKa = 11.84LKK13 pKa = 10.38RR14 pKa = 11.84KK15 pKa = 8.07RR16 pKa = 11.84QRR18 pKa = 11.84EE19 pKa = 4.07RR20 pKa = 11.84KK21 pKa = 9.69RR22 pKa = 11.84MEE24 pKa = 4.09KK25 pKa = 10.18NLSKK29 pKa = 10.51MSVKK33 pKa = 10.33SEE35 pKa = 3.8KK36 pKa = 10.63AQAAGTAGYY45 pKa = 9.66IFKK48 pKa = 9.42GTDD51 pKa = 2.94LVASVTVPQTAATGTIIARR70 pKa = 11.84LDD72 pKa = 3.14INPAKK77 pKa = 10.36FAGTTLAKK85 pKa = 10.13QASLWSNWIPRR96 pKa = 11.84KK97 pKa = 9.79LQVEE101 pKa = 4.42VQPSAGTTTSGTYY114 pKa = 10.19LVGWTLDD121 pKa = 3.44SALDD125 pKa = 3.58IPSGVNAIRR134 pKa = 11.84AVSAFEE140 pKa = 4.38KK141 pKa = 10.25PEE143 pKa = 3.78KK144 pKa = 10.11PRR146 pKa = 11.84IFDD149 pKa = 3.42KK150 pKa = 11.07VKK152 pKa = 10.78YY153 pKa = 10.1NISCKK158 pKa = 9.06MVQKK162 pKa = 10.27MLFCDD167 pKa = 4.91GSRR170 pKa = 11.84EE171 pKa = 4.25DD172 pKa = 3.99SDD174 pKa = 4.02QGTLYY179 pKa = 10.19IVLSSGIGNLVAGSVLTFNVYY200 pKa = 10.72LNYY203 pKa = 10.6DD204 pKa = 3.18IEE206 pKa = 4.83FYY208 pKa = 11.26NRR210 pKa = 11.84LATPASEE217 pKa = 4.27EE218 pKa = 4.02VLIYY222 pKa = 9.84PQAGYY227 pKa = 10.94EE228 pKa = 4.04NYY230 pKa = 8.49FTDD233 pKa = 5.36SSSDD237 pKa = 3.07WAGGTKK243 pKa = 10.48LSMKK247 pKa = 9.87QKK249 pKa = 10.41EE250 pKa = 4.46GGALVPWDD258 pKa = 3.65GAVPEE263 pKa = 5.39AIYY266 pKa = 10.25QFKK269 pKa = 9.03GTSLQYY275 pKa = 11.03YY276 pKa = 9.4DD277 pKa = 5.06AEE279 pKa = 4.51SALKK283 pKa = 10.08PITHH287 pKa = 7.46AVRR290 pKa = 11.84IRR292 pKa = 11.84DD293 pKa = 3.69RR294 pKa = 11.84GDD296 pKa = 2.84GALAVFEE303 pKa = 4.88SLAKK307 pKa = 9.62AQAYY311 pKa = 7.08ATTGDD316 pKa = 3.67EE317 pKa = 4.71VNCLTYY323 pKa = 10.33KK324 pKa = 10.02QAGPVVTPAGAPFMLVNPPPIFSNSLL350 pKa = 3.18

Molecular weight: 38.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1311

350

961

655.5

73.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.865 ± 2.283

1.602 ± 0.373

5.492 ± 0.461

9.153 ± 2.149

3.966 ± 0.269

7.094 ± 0.167

0.686 ± 0.201

3.738 ± 0.56

8.085 ± 0.329

8.543 ± 0.415

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.983 ± 0.008

3.356 ± 0.179

5.492 ± 0.175

3.966 ± 0.16

5.645 ± 0.537

6.407 ± 0.654

5.645 ± 0.75

7.399 ± 0.271

1.754 ± 0.163

3.127 ± 0.58
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski