Amino acid dipepetide frequency for Rhodococcus phage ChewyVIII

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.119AlaAla: 10.119 ± 0.921
0.416AlaCys: 0.416 ± 0.161
5.961AlaAsp: 5.961 ± 0.459
8.271AlaGlu: 8.271 ± 0.644
2.726AlaPhe: 2.726 ± 0.284
7.208AlaGly: 7.208 ± 0.478
1.941AlaHis: 1.941 ± 0.294
4.759AlaIle: 4.759 ± 0.411
4.944AlaLys: 4.944 ± 0.543
6.7AlaLeu: 6.7 ± 0.486
3.096AlaMet: 3.096 ± 0.383
3.327AlaAsn: 3.327 ± 0.385
3.142AlaPro: 3.142 ± 0.364
3.604AlaGln: 3.604 ± 0.424
5.083AlaArg: 5.083 ± 0.401
5.129AlaSer: 5.129 ± 0.386
4.898AlaThr: 4.898 ± 0.593
6.746AlaVal: 6.746 ± 0.546
1.34AlaTrp: 1.34 ± 0.267
2.033AlaTyr: 2.033 ± 0.289
0.0AlaXaa: 0.0 ± 0.0
Cys
0.647CysAla: 0.647 ± 0.175
0.046CysCys: 0.046 ± 0.05
0.462CysAsp: 0.462 ± 0.164
0.37CysGlu: 0.37 ± 0.114
0.139CysPhe: 0.139 ± 0.079
0.878CysGly: 0.878 ± 0.296
0.0CysHis: 0.0 ± 0.0
0.416CysIle: 0.416 ± 0.17
0.462CysLys: 0.462 ± 0.165
0.693CysLeu: 0.693 ± 0.162
0.092CysMet: 0.092 ± 0.057
0.554CysAsn: 0.554 ± 0.165
0.37CysPro: 0.37 ± 0.128
0.323CysGln: 0.323 ± 0.119
0.323CysArg: 0.323 ± 0.129
0.832CysSer: 0.832 ± 0.21
0.231CysThr: 0.231 ± 0.091
0.554CysVal: 0.554 ± 0.163
0.046CysTrp: 0.046 ± 0.046
0.37CysTyr: 0.37 ± 0.104
0.0CysXaa: 0.0 ± 0.0
Asp
6.423AspAla: 6.423 ± 0.576
0.462AspCys: 0.462 ± 0.164
3.974AspAsp: 3.974 ± 0.477
6.7AspGlu: 6.7 ± 0.734
2.033AspPhe: 2.033 ± 0.314
6.007AspGly: 6.007 ± 0.474
1.432AspHis: 1.432 ± 0.264
3.974AspIle: 3.974 ± 0.347
3.188AspLys: 3.188 ± 0.308
5.637AspLeu: 5.637 ± 0.518
1.525AspMet: 1.525 ± 0.29
2.541AspAsn: 2.541 ± 0.293
4.159AspPro: 4.159 ± 0.475
2.819AspGln: 2.819 ± 0.403
4.482AspArg: 4.482 ± 0.419
3.512AspSer: 3.512 ± 0.404
3.419AspThr: 3.419 ± 0.481
3.697AspVal: 3.697 ± 0.361
1.756AspTrp: 1.756 ± 0.258
2.68AspTyr: 2.68 ± 0.472
0.0AspXaa: 0.0 ± 0.0
Glu
7.07GluAla: 7.07 ± 0.589
0.508GluCys: 0.508 ± 0.158
5.73GluAsp: 5.73 ± 0.606
5.73GluGlu: 5.73 ± 0.588
2.541GluPhe: 2.541 ± 0.289
4.944GluGly: 4.944 ± 0.618
1.525GluHis: 1.525 ± 0.312
3.465GluIle: 3.465 ± 0.376
4.066GluLys: 4.066 ± 0.568
5.776GluLeu: 5.776 ± 0.718
1.987GluMet: 1.987 ± 0.311
2.495GluAsn: 2.495 ± 0.346
2.495GluPro: 2.495 ± 0.353
3.465GluGln: 3.465 ± 0.46
5.175GluArg: 5.175 ± 0.588
4.251GluSer: 4.251 ± 0.479
4.02GluThr: 4.02 ± 0.483
5.36GluVal: 5.36 ± 0.522
1.386GluTrp: 1.386 ± 0.28
2.172GluTyr: 2.172 ± 0.339
0.0GluXaa: 0.0 ± 0.0
Phe
3.188PheAla: 3.188 ± 0.428
0.37PheCys: 0.37 ± 0.115
3.188PheAsp: 3.188 ± 0.375
2.31PheGlu: 2.31 ± 0.372
0.924PhePhe: 0.924 ± 0.231
3.05PheGly: 3.05 ± 0.526
0.832PheHis: 0.832 ± 0.175
1.432PheIle: 1.432 ± 0.248
0.97PheLys: 0.97 ± 0.244
2.403PheLeu: 2.403 ± 0.314
0.924PheMet: 0.924 ± 0.213
0.786PheAsn: 0.786 ± 0.205
1.848PhePro: 1.848 ± 0.249
1.248PheGln: 1.248 ± 0.279
1.987PheArg: 1.987 ± 0.353
2.264PheSer: 2.264 ± 0.354
1.802PheThr: 1.802 ± 0.247
2.079PheVal: 2.079 ± 0.262
0.554PheTrp: 0.554 ± 0.155
1.802PheTyr: 1.802 ± 0.285
0.0PheXaa: 0.0 ± 0.0
Gly
6.284GlyAla: 6.284 ± 0.602
0.601GlyCys: 0.601 ± 0.192
6.33GlyAsp: 6.33 ± 1.232
5.36GlyGlu: 5.36 ± 0.54
4.297GlyPhe: 4.297 ± 0.454
5.268GlyGly: 5.268 ± 0.898
1.155GlyHis: 1.155 ± 0.261
4.805GlyIle: 4.805 ± 0.794
4.805GlyLys: 4.805 ± 0.478
6.376GlyLeu: 6.376 ± 0.542
1.71GlyMet: 1.71 ± 0.288
3.096GlyAsn: 3.096 ± 0.323
3.604GlyPro: 3.604 ± 0.513
3.835GlyGln: 3.835 ± 0.511
4.574GlyArg: 4.574 ± 0.46
5.037GlySer: 5.037 ± 0.512
4.759GlyThr: 4.759 ± 0.531
5.545GlyVal: 5.545 ± 0.56
1.386GlyTrp: 1.386 ± 0.225
2.31GlyTyr: 2.31 ± 0.286
0.0GlyXaa: 0.0 ± 0.0
His
1.71HisAla: 1.71 ± 0.352
0.185HisCys: 0.185 ± 0.094
1.109HisAsp: 1.109 ± 0.286
1.201HisGlu: 1.201 ± 0.31
0.693HisPhe: 0.693 ± 0.191
1.663HisGly: 1.663 ± 0.321
0.508HisHis: 0.508 ± 0.156
0.647HisIle: 0.647 ± 0.171
0.97HisLys: 0.97 ± 0.189
1.802HisLeu: 1.802 ± 0.272
0.37HisMet: 0.37 ± 0.128
0.739HisAsn: 0.739 ± 0.178
1.34HisPro: 1.34 ± 0.235
0.97HisGln: 0.97 ± 0.22
1.34HisArg: 1.34 ± 0.233
0.739HisSer: 0.739 ± 0.178
1.294HisThr: 1.294 ± 0.273
0.878HisVal: 0.878 ± 0.198
0.601HisTrp: 0.601 ± 0.164
0.97HisTyr: 0.97 ± 0.214
0.0HisXaa: 0.0 ± 0.0
Ile
5.406IleAla: 5.406 ± 0.503
0.37IleCys: 0.37 ± 0.126
4.482IleAsp: 4.482 ± 0.453
3.743IleGlu: 3.743 ± 0.43
1.756IlePhe: 1.756 ± 0.295
3.697IleGly: 3.697 ± 0.543
1.479IleHis: 1.479 ± 0.22
1.894IleIle: 1.894 ± 0.287
1.756IleLys: 1.756 ± 0.301
3.142IleLeu: 3.142 ± 0.375
1.432IleMet: 1.432 ± 0.261
2.218IleAsn: 2.218 ± 0.372
2.588IlePro: 2.588 ± 0.284
2.033IleGln: 2.033 ± 0.467
3.465IleArg: 3.465 ± 0.343
2.218IleSer: 2.218 ± 0.308
3.096IleThr: 3.096 ± 0.341
3.697IleVal: 3.697 ± 0.55
0.647IleTrp: 0.647 ± 0.163
1.155IleTyr: 1.155 ± 0.225
0.0IleXaa: 0.0 ± 0.0
Lys
4.805LysAla: 4.805 ± 0.511
0.277LysCys: 0.277 ± 0.107
2.819LysAsp: 2.819 ± 0.376
3.234LysGlu: 3.234 ± 0.442
2.172LysPhe: 2.172 ± 0.231
4.297LysGly: 4.297 ± 1.0
1.109LysHis: 1.109 ± 0.257
2.541LysIle: 2.541 ± 0.325
3.419LysLys: 3.419 ± 0.55
3.743LysLeu: 3.743 ± 0.37
1.756LysMet: 1.756 ± 0.245
1.848LysAsn: 1.848 ± 0.299
2.634LysPro: 2.634 ± 0.39
2.125LysGln: 2.125 ± 0.33
3.003LysArg: 3.003 ± 0.463
2.125LysSer: 2.125 ± 0.285
3.512LysThr: 3.512 ± 0.428
3.419LysVal: 3.419 ± 0.399
0.693LysTrp: 0.693 ± 0.188
1.432LysTyr: 1.432 ± 0.221
0.0LysXaa: 0.0 ± 0.0
Leu
6.053LeuAla: 6.053 ± 0.527
0.878LeuCys: 0.878 ± 0.254
6.145LeuAsp: 6.145 ± 0.551
6.284LeuGlu: 6.284 ± 0.559
2.357LeuPhe: 2.357 ± 0.242
5.73LeuGly: 5.73 ± 0.525
1.756LeuHis: 1.756 ± 0.241
3.881LeuIle: 3.881 ± 0.41
3.188LeuLys: 3.188 ± 0.472
5.961LeuLeu: 5.961 ± 0.526
1.617LeuMet: 1.617 ± 0.265
2.495LeuAsn: 2.495 ± 0.368
4.482LeuPro: 4.482 ± 0.466
2.957LeuGln: 2.957 ± 0.333
4.667LeuArg: 4.667 ± 0.59
4.205LeuSer: 4.205 ± 0.494
3.743LeuThr: 3.743 ± 0.495
5.683LeuVal: 5.683 ± 0.511
1.617LeuTrp: 1.617 ± 0.269
2.079LeuTyr: 2.079 ± 0.334
0.0LeuXaa: 0.0 ± 0.0
Met
3.604MetAla: 3.604 ± 0.379
0.046MetCys: 0.046 ± 0.047
1.294MetAsp: 1.294 ± 0.248
1.294MetGlu: 1.294 ± 0.263
0.601MetPhe: 0.601 ± 0.175
1.386MetGly: 1.386 ± 0.351
0.601MetHis: 0.601 ± 0.155
1.525MetIle: 1.525 ± 0.257
1.848MetLys: 1.848 ± 0.24
2.218MetLeu: 2.218 ± 0.272
0.508MetMet: 0.508 ± 0.167
1.201MetAsn: 1.201 ± 0.25
1.571MetPro: 1.571 ± 0.255
1.017MetGln: 1.017 ± 0.219
1.386MetArg: 1.386 ± 0.274
2.357MetSer: 2.357 ± 0.313
2.31MetThr: 2.31 ± 0.273
1.109MetVal: 1.109 ± 0.2
0.323MetTrp: 0.323 ± 0.107
0.601MetTyr: 0.601 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
2.772AsnAla: 2.772 ± 0.363
0.37AsnCys: 0.37 ± 0.126
2.911AsnAsp: 2.911 ± 0.358
1.987AsnGlu: 1.987 ± 0.282
1.155AsnPhe: 1.155 ± 0.187
3.465AsnGly: 3.465 ± 0.412
1.109AsnHis: 1.109 ± 0.277
1.34AsnIle: 1.34 ± 0.255
1.71AsnLys: 1.71 ± 0.302
2.819AsnLeu: 2.819 ± 0.301
1.063AsnMet: 1.063 ± 0.234
1.109AsnAsn: 1.109 ± 0.33
2.865AsnPro: 2.865 ± 0.307
1.063AsnGln: 1.063 ± 0.218
2.125AsnArg: 2.125 ± 0.287
2.079AsnSer: 2.079 ± 0.264
2.033AsnThr: 2.033 ± 0.346
2.172AsnVal: 2.172 ± 0.284
0.878AsnTrp: 0.878 ± 0.164
1.34AsnTyr: 1.34 ± 0.246
0.0AsnXaa: 0.0 ± 0.0
Pro
4.39ProAla: 4.39 ± 0.47
0.139ProCys: 0.139 ± 0.082
4.436ProAsp: 4.436 ± 0.485
4.39ProGlu: 4.39 ± 0.364
1.063ProPhe: 1.063 ± 0.236
4.297ProGly: 4.297 ± 0.496
0.462ProHis: 0.462 ± 0.169
2.541ProIle: 2.541 ± 0.28
3.003ProLys: 3.003 ± 0.42
3.512ProLeu: 3.512 ± 0.35
1.109ProMet: 1.109 ± 0.179
2.218ProAsn: 2.218 ± 0.263
2.403ProPro: 2.403 ± 0.393
1.848ProGln: 1.848 ± 0.308
3.281ProArg: 3.281 ± 0.458
3.05ProSer: 3.05 ± 0.343
3.096ProThr: 3.096 ± 0.552
2.865ProVal: 2.865 ± 0.275
0.739ProTrp: 0.739 ± 0.163
1.248ProTyr: 1.248 ± 0.191
0.0ProXaa: 0.0 ± 0.0
Gln
3.974GlnAla: 3.974 ± 0.45
0.231GlnCys: 0.231 ± 0.094
1.941GlnAsp: 1.941 ± 0.322
2.125GlnGlu: 2.125 ± 0.275
1.617GlnPhe: 1.617 ± 0.252
3.928GlnGly: 3.928 ± 1.261
0.832GlnHis: 0.832 ± 0.209
2.31GlnIle: 2.31 ± 0.242
1.941GlnLys: 1.941 ± 0.323
2.68GlnLeu: 2.68 ± 0.395
0.878GlnMet: 0.878 ± 0.205
1.155GlnAsn: 1.155 ± 0.212
1.894GlnPro: 1.894 ± 0.339
1.71GlnGln: 1.71 ± 0.344
2.68GlnArg: 2.68 ± 0.408
2.079GlnSer: 2.079 ± 0.321
2.125GlnThr: 2.125 ± 0.323
2.634GlnVal: 2.634 ± 0.401
0.693GlnTrp: 0.693 ± 0.171
0.878GlnTyr: 0.878 ± 0.195
0.0GlnXaa: 0.0 ± 0.0
Arg
5.129ArgAla: 5.129 ± 0.495
0.739ArgCys: 0.739 ± 0.205
4.159ArgAsp: 4.159 ± 0.392
4.39ArgGlu: 4.39 ± 0.457
2.357ArgPhe: 2.357 ± 0.324
4.99ArgGly: 4.99 ± 0.481
1.248ArgHis: 1.248 ± 0.251
3.373ArgIle: 3.373 ± 0.376
3.419ArgLys: 3.419 ± 0.379
5.083ArgLeu: 5.083 ± 0.456
2.449ArgMet: 2.449 ± 0.336
2.495ArgAsn: 2.495 ± 0.323
2.726ArgPro: 2.726 ± 0.418
1.525ArgGln: 1.525 ± 0.265
5.037ArgArg: 5.037 ± 0.474
3.142ArgSer: 3.142 ± 0.345
3.881ArgThr: 3.881 ± 0.413
4.205ArgVal: 4.205 ± 0.454
1.063ArgTrp: 1.063 ± 0.287
2.079ArgTyr: 2.079 ± 0.31
0.0ArgXaa: 0.0 ± 0.0
Ser
5.083SerAla: 5.083 ± 0.452
0.508SerCys: 0.508 ± 0.133
3.558SerAsp: 3.558 ± 0.408
3.65SerGlu: 3.65 ± 0.436
1.756SerPhe: 1.756 ± 0.24
5.914SerGly: 5.914 ± 0.591
0.924SerHis: 0.924 ± 0.205
2.541SerIle: 2.541 ± 0.307
3.234SerLys: 3.234 ± 0.456
3.743SerLeu: 3.743 ± 0.383
1.941SerMet: 1.941 ± 0.225
1.802SerAsn: 1.802 ± 0.31
3.419SerPro: 3.419 ± 0.345
1.663SerGln: 1.663 ± 0.282
2.819SerArg: 2.819 ± 0.432
3.096SerSer: 3.096 ± 0.385
3.743SerThr: 3.743 ± 0.431
3.881SerVal: 3.881 ± 0.426
1.017SerTrp: 1.017 ± 0.219
1.617SerTyr: 1.617 ± 0.281
0.0SerXaa: 0.0 ± 0.0
Thr
5.083ThrAla: 5.083 ± 0.453
0.416ThrCys: 0.416 ± 0.182
3.881ThrAsp: 3.881 ± 0.393
3.743ThrGlu: 3.743 ± 0.37
1.802ThrPhe: 1.802 ± 0.301
5.683ThrGly: 5.683 ± 0.694
0.924ThrHis: 0.924 ± 0.189
3.697ThrIle: 3.697 ± 0.419
2.911ThrLys: 2.911 ± 0.482
4.112ThrLeu: 4.112 ± 0.412
1.571ThrMet: 1.571 ± 0.248
2.218ThrAsn: 2.218 ± 0.348
3.327ThrPro: 3.327 ± 0.375
1.848ThrGln: 1.848 ± 0.293
4.297ThrArg: 4.297 ± 0.431
3.003ThrSer: 3.003 ± 0.413
3.974ThrThr: 3.974 ± 0.49
3.604ThrVal: 3.604 ± 0.473
1.294ThrTrp: 1.294 ± 0.196
1.756ThrTyr: 1.756 ± 0.254
0.0ThrXaa: 0.0 ± 0.0
Val
6.099ValAla: 6.099 ± 0.555
0.693ValCys: 0.693 ± 0.189
4.667ValAsp: 4.667 ± 0.417
5.545ValGlu: 5.545 ± 0.763
2.68ValPhe: 2.68 ± 0.327
5.268ValGly: 5.268 ± 0.586
1.063ValHis: 1.063 ± 0.25
3.512ValIle: 3.512 ± 0.47
3.188ValLys: 3.188 ± 0.37
5.037ValLeu: 5.037 ± 0.439
1.386ValMet: 1.386 ± 0.262
2.264ValAsn: 2.264 ± 0.279
2.911ValPro: 2.911 ± 0.323
2.495ValGln: 2.495 ± 0.326
4.159ValArg: 4.159 ± 0.367
4.02ValSer: 4.02 ± 0.443
3.789ValThr: 3.789 ± 0.385
4.02ValVal: 4.02 ± 0.419
1.294ValTrp: 1.294 ± 0.26
1.848ValTyr: 1.848 ± 0.326
0.0ValXaa: 0.0 ± 0.0
Trp
1.386TrpAla: 1.386 ± 0.306
0.139TrpCys: 0.139 ± 0.084
1.34TrpAsp: 1.34 ± 0.24
1.756TrpGlu: 1.756 ± 0.239
0.601TrpPhe: 0.601 ± 0.194
1.109TrpGly: 1.109 ± 0.198
0.416TrpHis: 0.416 ± 0.142
0.416TrpIle: 0.416 ± 0.135
0.786TrpLys: 0.786 ± 0.169
1.848TrpLeu: 1.848 ± 0.284
0.647TrpMet: 0.647 ± 0.146
0.924TrpAsn: 0.924 ± 0.239
0.416TrpPro: 0.416 ± 0.143
0.739TrpGln: 0.739 ± 0.173
0.97TrpArg: 0.97 ± 0.194
0.832TrpSer: 0.832 ± 0.22
1.525TrpThr: 1.525 ± 0.301
1.663TrpVal: 1.663 ± 0.264
0.554TrpTrp: 0.554 ± 0.157
0.554TrpTyr: 0.554 ± 0.172
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.218TyrAla: 2.218 ± 0.277
0.416TyrCys: 0.416 ± 0.14
2.033TyrAsp: 2.033 ± 0.324
2.079TyrGlu: 2.079 ± 0.346
0.693TyrPhe: 0.693 ± 0.172
2.357TyrGly: 2.357 ± 0.285
0.416TyrHis: 0.416 ± 0.148
1.201TyrIle: 1.201 ± 0.233
1.155TyrLys: 1.155 ± 0.274
2.541TyrLeu: 2.541 ± 0.377
0.693TyrMet: 0.693 ± 0.165
0.878TyrAsn: 0.878 ± 0.237
1.894TyrPro: 1.894 ± 0.259
1.017TyrGln: 1.017 ± 0.221
2.634TyrArg: 2.634 ± 0.305
1.941TyrSer: 1.941 ± 0.332
1.894TyrThr: 1.894 ± 0.288
2.125TyrVal: 2.125 ± 0.337
0.739TyrTrp: 0.739 ± 0.161
0.739TyrTyr: 0.739 ± 0.161
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (21643 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski