Amino acid dipepetide frequency for Bacillus alcalophilus ATCC 27647 = CGMCC 1.3604

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.105AlaAla: 5.105 ± 0.092
0.62AlaCys: 0.62 ± 0.025
3.222AlaAsp: 3.222 ± 0.053
4.791AlaGlu: 4.791 ± 0.076
3.288AlaPhe: 3.288 ± 0.068
4.986AlaGly: 4.986 ± 0.08
1.292AlaHis: 1.292 ± 0.038
5.886AlaIle: 5.886 ± 0.092
4.675AlaLys: 4.675 ± 0.084
7.26AlaLeu: 7.26 ± 0.099
1.887AlaMet: 1.887 ± 0.045
2.799AlaAsn: 2.799 ± 0.057
1.972AlaPro: 1.972 ± 0.04
2.231AlaGln: 2.231 ± 0.049
2.534AlaArg: 2.534 ± 0.059
3.778AlaSer: 3.778 ± 0.063
3.658AlaThr: 3.658 ± 0.068
4.936AlaVal: 4.936 ± 0.081
0.568AlaTrp: 0.568 ± 0.024
2.268AlaTyr: 2.268 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.415CysAla: 0.415 ± 0.023
0.077CysCys: 0.077 ± 0.009
0.346CysAsp: 0.346 ± 0.018
0.494CysGlu: 0.494 ± 0.025
0.291CysPhe: 0.291 ± 0.018
0.64CysGly: 0.64 ± 0.026
0.205CysHis: 0.205 ± 0.013
0.458CysIle: 0.458 ± 0.02
0.312CysLys: 0.312 ± 0.015
0.719CysLeu: 0.719 ± 0.025
0.149CysMet: 0.149 ± 0.012
0.268CysAsn: 0.268 ± 0.015
0.363CysPro: 0.363 ± 0.019
0.298CysGln: 0.298 ± 0.016
0.265CysArg: 0.265 ± 0.019
0.546CysSer: 0.546 ± 0.026
0.367CysThr: 0.367 ± 0.025
0.376CysVal: 0.376 ± 0.02
0.062CysTrp: 0.062 ± 0.008
0.251CysTyr: 0.251 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
2.915AspAla: 2.915 ± 0.055
0.335AspCys: 0.335 ± 0.018
2.497AspAsp: 2.497 ± 0.052
4.747AspGlu: 4.747 ± 0.078
2.573AspPhe: 2.573 ± 0.059
3.339AspGly: 3.339 ± 0.068
1.119AspHis: 1.119 ± 0.037
3.732AspIle: 3.732 ± 0.063
2.901AspLys: 2.901 ± 0.058
5.102AspLeu: 5.102 ± 0.071
1.259AspMet: 1.259 ± 0.041
1.817AspAsn: 1.817 ± 0.048
1.959AspPro: 1.959 ± 0.05
1.807AspGln: 1.807 ± 0.045
2.032AspArg: 2.032 ± 0.05
2.662AspSer: 2.662 ± 0.057
2.226AspThr: 2.226 ± 0.044
3.828AspVal: 3.828 ± 0.059
0.671AspTrp: 0.671 ± 0.024
2.264AspTyr: 2.264 ± 0.059
0.0AspXaa: 0.0 ± 0.0
Glu
5.862GluAla: 5.862 ± 0.084
0.384GluCys: 0.384 ± 0.02
3.862GluAsp: 3.862 ± 0.063
8.066GluGlu: 8.066 ± 0.126
2.64GluPhe: 2.64 ± 0.05
4.595GluGly: 4.595 ± 0.072
1.744GluHis: 1.744 ± 0.046
5.759GluIle: 5.759 ± 0.074
6.637GluLys: 6.637 ± 0.094
8.084GluLeu: 8.084 ± 0.098
2.462GluMet: 2.462 ± 0.048
3.606GluAsn: 3.606 ± 0.064
2.219GluPro: 2.219 ± 0.051
4.661GluGln: 4.661 ± 0.078
4.099GluArg: 4.099 ± 0.068
3.828GluSer: 3.828 ± 0.061
4.044GluThr: 4.044 ± 0.064
5.995GluVal: 5.995 ± 0.083
0.883GluTrp: 0.883 ± 0.032
2.235GluTyr: 2.235 ± 0.052
0.0GluXaa: 0.0 ± 0.0
Phe
3.056PheAla: 3.056 ± 0.058
0.327PheCys: 0.327 ± 0.016
2.368PheAsp: 2.368 ± 0.045
3.242PheGlu: 3.242 ± 0.059
2.486PhePhe: 2.486 ± 0.071
3.116PheGly: 3.116 ± 0.064
0.985PheHis: 0.985 ± 0.031
4.084PheIle: 4.084 ± 0.088
2.354PheLys: 2.354 ± 0.049
4.71PheLeu: 4.71 ± 0.081
1.133PheMet: 1.133 ± 0.033
1.951PheAsn: 1.951 ± 0.046
1.648PhePro: 1.648 ± 0.045
1.784PheGln: 1.784 ± 0.043
1.584PheArg: 1.584 ± 0.037
3.22PheSer: 3.22 ± 0.063
2.471PheThr: 2.471 ± 0.054
3.353PheVal: 3.353 ± 0.072
0.49PheTrp: 0.49 ± 0.022
1.844PheTyr: 1.844 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
4.843GlyAla: 4.843 ± 0.086
0.63GlyCys: 0.63 ± 0.025
3.09GlyAsp: 3.09 ± 0.064
4.795GlyGlu: 4.795 ± 0.076
3.418GlyPhe: 3.418 ± 0.065
4.57GlyGly: 4.57 ± 0.081
1.497GlyHis: 1.497 ± 0.041
5.307GlyIle: 5.307 ± 0.078
4.433GlyLys: 4.433 ± 0.072
6.807GlyLeu: 6.807 ± 0.081
1.987GlyMet: 1.987 ± 0.044
2.461GlyAsn: 2.461 ± 0.053
1.742GlyPro: 1.742 ± 0.047
2.461GlyGln: 2.461 ± 0.051
2.606GlyArg: 2.606 ± 0.055
3.692GlySer: 3.692 ± 0.065
3.697GlyThr: 3.697 ± 0.069
5.062GlyVal: 5.062 ± 0.068
0.763GlyTrp: 0.763 ± 0.029
2.701GlyTyr: 2.701 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
1.218HisAla: 1.218 ± 0.038
0.184HisCys: 0.184 ± 0.016
1.031HisAsp: 1.031 ± 0.033
1.506HisGlu: 1.506 ± 0.039
1.179HisPhe: 1.179 ± 0.033
1.301HisGly: 1.301 ± 0.038
0.656HisHis: 0.656 ± 0.027
1.508HisIle: 1.508 ± 0.04
1.049HisLys: 1.049 ± 0.033
2.401HisLeu: 2.401 ± 0.053
0.495HisMet: 0.495 ± 0.022
0.815HisAsn: 0.815 ± 0.03
1.039HisPro: 1.039 ± 0.036
0.968HisGln: 0.968 ± 0.032
0.848HisArg: 0.848 ± 0.028
1.369HisSer: 1.369 ± 0.037
1.016HisThr: 1.016 ± 0.03
1.429HisVal: 1.429 ± 0.037
0.219HisTrp: 0.219 ± 0.014
0.942HisTyr: 0.942 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
5.78IleAla: 5.78 ± 0.089
0.662IleCys: 0.662 ± 0.028
4.451IleAsp: 4.451 ± 0.068
6.334IleGlu: 6.334 ± 0.095
3.274IlePhe: 3.274 ± 0.066
5.879IleGly: 5.879 ± 0.076
1.669IleHis: 1.669 ± 0.039
5.85IleIle: 5.85 ± 0.089
4.464IleLys: 4.464 ± 0.076
6.869IleLeu: 6.869 ± 0.103
1.766IleMet: 1.766 ± 0.046
3.31IleAsn: 3.31 ± 0.061
3.222IlePro: 3.222 ± 0.058
2.979IleGln: 2.979 ± 0.067
3.144IleArg: 3.144 ± 0.056
4.862IleSer: 4.862 ± 0.073
4.017IleThr: 4.017 ± 0.067
5.451IleVal: 5.451 ± 0.076
0.706IleTrp: 0.706 ± 0.026
2.527IleTyr: 2.527 ± 0.048
0.0IleXaa: 0.0 ± 0.0
Lys
4.437LysAla: 4.437 ± 0.067
0.297LysCys: 0.297 ± 0.017
3.155LysAsp: 3.155 ± 0.061
7.274LysGlu: 7.274 ± 0.1
1.854LysPhe: 1.854 ± 0.046
4.321LysGly: 4.321 ± 0.065
1.288LysHis: 1.288 ± 0.036
4.277LysIle: 4.277 ± 0.061
6.013LysLys: 6.013 ± 0.082
5.759LysLeu: 5.759 ± 0.08
2.169LysMet: 2.169 ± 0.046
2.936LysAsn: 2.936 ± 0.058
2.092LysPro: 2.092 ± 0.051
3.248LysGln: 3.248 ± 0.063
3.524LysArg: 3.524 ± 0.056
3.408LysSer: 3.408 ± 0.054
3.296LysThr: 3.296 ± 0.06
4.712LysVal: 4.712 ± 0.072
0.848LysTrp: 0.848 ± 0.026
1.904LysTyr: 1.904 ± 0.045
0.0LysXaa: 0.0 ± 0.0
Leu
7.413LeuAla: 7.413 ± 0.108
0.646LeuCys: 0.646 ± 0.023
4.919LeuAsp: 4.919 ± 0.064
7.537LeuGlu: 7.537 ± 0.091
5.126LeuPhe: 5.126 ± 0.087
6.39LeuGly: 6.39 ± 0.091
2.05LeuHis: 2.05 ± 0.044
7.833LeuIle: 7.833 ± 0.119
6.757LeuLys: 6.757 ± 0.094
10.626LeuLeu: 10.626 ± 0.145
2.63LeuMet: 2.63 ± 0.056
4.502LeuAsn: 4.502 ± 0.066
3.832LeuPro: 3.832 ± 0.062
3.771LeuGln: 3.771 ± 0.065
3.67LeuArg: 3.67 ± 0.068
6.716LeuSer: 6.716 ± 0.09
5.851LeuThr: 5.851 ± 0.085
6.683LeuVal: 6.683 ± 0.083
0.816LeuTrp: 0.816 ± 0.029
3.173LeuTyr: 3.173 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
1.924MetAla: 1.924 ± 0.044
0.126MetCys: 0.126 ± 0.012
1.372MetAsp: 1.372 ± 0.036
2.021MetGlu: 2.021 ± 0.043
1.054MetPhe: 1.054 ± 0.034
1.554MetGly: 1.554 ± 0.04
0.408MetHis: 0.408 ± 0.022
2.217MetIle: 2.217 ± 0.051
2.37MetLys: 2.37 ± 0.049
2.578MetLeu: 2.578 ± 0.054
0.864MetMet: 0.864 ± 0.031
1.633MetAsn: 1.633 ± 0.039
0.912MetPro: 0.912 ± 0.032
0.818MetGln: 0.818 ± 0.028
1.079MetArg: 1.079 ± 0.031
1.792MetSer: 1.792 ± 0.037
1.716MetThr: 1.716 ± 0.039
1.793MetVal: 1.793 ± 0.041
0.199MetTrp: 0.199 ± 0.014
0.768MetTyr: 0.768 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
2.409AsnAla: 2.409 ± 0.049
0.272AsnCys: 0.272 ± 0.017
2.233AsnAsp: 2.233 ± 0.053
4.095AsnGlu: 4.095 ± 0.07
1.542AsnPhe: 1.542 ± 0.036
3.237AsnGly: 3.237 ± 0.061
1.152AsnHis: 1.152 ± 0.037
3.089AsnIle: 3.089 ± 0.05
2.874AsnLys: 2.874 ± 0.056
3.826AsnLeu: 3.826 ± 0.064
1.148AsnMet: 1.148 ± 0.032
1.818AsnAsn: 1.818 ± 0.048
1.974AsnPro: 1.974 ± 0.047
2.357AsnGln: 2.357 ± 0.049
2.087AsnArg: 2.087 ± 0.045
2.183AsnSer: 2.183 ± 0.049
1.923AsnThr: 1.923 ± 0.042
2.879AsnVal: 2.879 ± 0.05
0.596AsnTrp: 0.596 ± 0.025
1.555AsnTyr: 1.555 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
2.088ProAla: 2.088 ± 0.049
0.203ProCys: 0.203 ± 0.015
1.833ProAsp: 1.833 ± 0.049
2.928ProGlu: 2.928 ± 0.056
2.045ProPhe: 2.045 ± 0.049
2.11ProGly: 2.11 ± 0.045
0.796ProHis: 0.796 ± 0.027
2.776ProIle: 2.776 ± 0.051
2.101ProLys: 2.101 ± 0.047
3.672ProLeu: 3.672 ± 0.059
0.776ProMet: 0.776 ± 0.029
1.764ProAsn: 1.764 ± 0.042
0.976ProPro: 0.976 ± 0.031
1.058ProGln: 1.058 ± 0.031
1.097ProArg: 1.097 ± 0.033
2.138ProSer: 2.138 ± 0.053
2.099ProThr: 2.099 ± 0.053
2.583ProVal: 2.583 ± 0.059
0.365ProTrp: 0.365 ± 0.019
1.338ProTyr: 1.338 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
2.886GlnAla: 2.886 ± 0.063
0.218GlnCys: 0.218 ± 0.013
1.601GlnAsp: 1.601 ± 0.038
3.148GlnGlu: 3.148 ± 0.069
1.828GlnPhe: 1.828 ± 0.044
2.161GlnGly: 2.161 ± 0.047
0.794GlnHis: 0.794 ± 0.028
2.985GlnIle: 2.985 ± 0.055
2.951GlnLys: 2.951 ± 0.054
4.786GlnLeu: 4.786 ± 0.071
1.225GlnMet: 1.225 ± 0.037
1.78GlnAsn: 1.78 ± 0.048
1.24GlnPro: 1.24 ± 0.045
1.952GlnGln: 1.952 ± 0.052
1.584GlnArg: 1.584 ± 0.046
2.471GlnSer: 2.471 ± 0.049
2.295GlnThr: 2.295 ± 0.044
2.781GlnVal: 2.781 ± 0.054
0.484GlnTrp: 0.484 ± 0.021
1.373GlnTyr: 1.373 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.554ArgAla: 2.554 ± 0.05
0.257ArgCys: 0.257 ± 0.017
1.977ArgAsp: 1.977 ± 0.044
3.34ArgGlu: 3.34 ± 0.066
2.166ArgPhe: 2.166 ± 0.051
2.352ArgGly: 2.352 ± 0.049
0.827ArgHis: 0.827 ± 0.028
3.096ArgIle: 3.096 ± 0.058
3.057ArgLys: 3.057 ± 0.061
4.266ArgLeu: 4.266 ± 0.071
1.257ArgMet: 1.257 ± 0.034
1.856ArgAsn: 1.856 ± 0.049
1.325ArgPro: 1.325 ± 0.04
1.591ArgGln: 1.591 ± 0.036
1.836ArgArg: 1.836 ± 0.048
2.314ArgSer: 2.314 ± 0.055
2.112ArgThr: 2.112 ± 0.04
2.69ArgVal: 2.69 ± 0.048
0.433ArgTrp: 0.433 ± 0.022
1.562ArgTyr: 1.562 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
3.478SerAla: 3.478 ± 0.059
0.394SerCys: 0.394 ± 0.02
2.881SerAsp: 2.881 ± 0.06
4.398SerGlu: 4.398 ± 0.07
3.397SerPhe: 3.397 ± 0.063
4.282SerGly: 4.282 ± 0.071
1.204SerHis: 1.204 ± 0.037
4.889SerIle: 4.889 ± 0.075
3.583SerLys: 3.583 ± 0.064
6.322SerLeu: 6.322 ± 0.078
1.634SerMet: 1.634 ± 0.044
2.564SerAsn: 2.564 ± 0.054
1.98SerPro: 1.98 ± 0.042
2.131SerGln: 2.131 ± 0.045
2.25SerArg: 2.25 ± 0.053
3.88SerSer: 3.88 ± 0.07
3.026SerThr: 3.026 ± 0.054
3.983SerVal: 3.983 ± 0.066
0.678SerTrp: 0.678 ± 0.024
2.266SerTyr: 2.266 ± 0.049
0.0SerXaa: 0.0 ± 0.0
Thr
3.544ThrAla: 3.544 ± 0.06
0.36ThrCys: 0.36 ± 0.02
2.724ThrAsp: 2.724 ± 0.048
3.802ThrGlu: 3.802 ± 0.059
2.566ThrPhe: 2.566 ± 0.047
3.864ThrGly: 3.864 ± 0.065
1.014ThrHis: 1.014 ± 0.032
4.592ThrIle: 4.592 ± 0.067
3.407ThrLys: 3.407 ± 0.058
5.418ThrLeu: 5.418 ± 0.09
1.278ThrMet: 1.278 ± 0.034
2.521ThrAsn: 2.521 ± 0.052
2.086ThrPro: 2.086 ± 0.043
1.417ThrGln: 1.417 ± 0.032
1.894ThrArg: 1.894 ± 0.04
3.153ThrSer: 3.153 ± 0.056
3.03ThrThr: 3.03 ± 0.061
4.079ThrVal: 4.079 ± 0.065
0.501ThrTrp: 0.501 ± 0.024
1.928ThrTyr: 1.928 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
5.24ValAla: 5.24 ± 0.066
0.539ValCys: 0.539 ± 0.02
3.818ValAsp: 3.818 ± 0.057
5.633ValGlu: 5.633 ± 0.091
3.07ValPhe: 3.07 ± 0.061
4.847ValGly: 4.847 ± 0.071
1.402ValHis: 1.402 ± 0.035
5.734ValIle: 5.734 ± 0.085
4.294ValLys: 4.294 ± 0.064
6.83ValLeu: 6.83 ± 0.081
1.891ValMet: 1.891 ± 0.051
2.957ValAsn: 2.957 ± 0.055
2.482ValPro: 2.482 ± 0.044
2.653ValGln: 2.653 ± 0.056
2.728ValArg: 2.728 ± 0.051
4.474ValSer: 4.474 ± 0.07
4.1ValThr: 4.1 ± 0.075
5.142ValVal: 5.142 ± 0.073
0.683ValTrp: 0.683 ± 0.025
2.16ValTyr: 2.16 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
0.579TrpAla: 0.579 ± 0.026
0.086TrpCys: 0.086 ± 0.009
0.514TrpAsp: 0.514 ± 0.025
0.707TrpGlu: 0.707 ± 0.03
0.585TrpPhe: 0.585 ± 0.028
0.655TrpGly: 0.655 ± 0.027
0.202TrpHis: 0.202 ± 0.014
0.789TrpIle: 0.789 ± 0.027
0.688TrpLys: 0.688 ± 0.026
1.226TrpLeu: 1.226 ± 0.036
0.351TrpMet: 0.351 ± 0.017
0.578TrpAsn: 0.578 ± 0.024
0.272TrpPro: 0.272 ± 0.016
0.47TrpGln: 0.47 ± 0.024
0.42TrpArg: 0.42 ± 0.02
0.627TrpSer: 0.627 ± 0.027
0.555TrpThr: 0.555 ± 0.024
0.691TrpVal: 0.691 ± 0.026
0.141TrpTrp: 0.141 ± 0.015
0.355TrpTyr: 0.355 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.01TyrAla: 2.01 ± 0.045
0.282TyrCys: 0.282 ± 0.017
1.893TyrAsp: 1.893 ± 0.046
2.809TyrGlu: 2.809 ± 0.052
1.824TyrPhe: 1.824 ± 0.05
2.364TyrGly: 2.364 ± 0.054
0.871TyrHis: 0.871 ± 0.03
2.355TyrIle: 2.355 ± 0.048
1.893TyrLys: 1.893 ± 0.046
3.676TyrLeu: 3.676 ± 0.071
0.834TyrMet: 0.834 ± 0.023
1.315TyrAsn: 1.315 ± 0.037
1.408TyrPro: 1.408 ± 0.035
1.89TyrGln: 1.89 ± 0.042
1.586TyrArg: 1.586 ± 0.039
2.107TyrSer: 2.107 ± 0.048
1.676TyrThr: 1.676 ± 0.04
2.236TyrVal: 2.236 ± 0.05
0.384TyrTrp: 0.384 ± 0.02
1.397TyrTyr: 1.397 ± 0.044
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3743 proteins (1064280 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski