Tortoise microvirus 19

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6X5|A0A4P8W6X5_9VIRU Replication initiation protein OS=Tortoise microvirus 19 OX=2583120 PE=4 SV=1
MM1 pKa = 7.77EE2 pKa = 5.19EE3 pKa = 4.07NEE5 pKa = 4.35KK6 pKa = 10.91QNSLGLSQDD15 pKa = 2.93KK16 pKa = 11.1ANYY19 pKa = 9.37SDD21 pKa = 3.7EE22 pKa = 4.23EE23 pKa = 4.53RR24 pKa = 11.84IEE26 pKa = 3.97MQRR29 pKa = 11.84GIVGTPLKK37 pKa = 10.27VVRR40 pKa = 11.84VEE42 pKa = 3.92EE43 pKa = 4.34TYY45 pKa = 10.64KK46 pKa = 10.52IAFGKK51 pKa = 10.86YY52 pKa = 8.28IVCSKK57 pKa = 10.95NFGTIEE63 pKa = 3.76EE64 pKa = 4.32AEE66 pKa = 4.4EE67 pKa = 4.02FVEE70 pKa = 4.65TKK72 pKa = 10.27PGLWDD77 pKa = 3.25IVVGLMGVATTEE89 pKa = 4.37AIKK92 pKa = 10.45EE93 pKa = 4.01ISRR96 pKa = 11.84EE97 pKa = 3.96LTDD100 pKa = 3.61EE101 pKa = 4.25LKK103 pKa = 10.76EE104 pKa = 4.06ITGEE108 pKa = 4.01QQ109 pKa = 3.26

Molecular weight: 12.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W9A8|A0A4P8W9A8_9VIRU DNA pilot protein OS=Tortoise microvirus 19 OX=2583120 PE=4 SV=1
MM1 pKa = 7.56CLYY4 pKa = 10.5TKK6 pKa = 9.98MVLNPKK12 pKa = 9.57YY13 pKa = 10.42KK14 pKa = 9.82PSKK17 pKa = 10.22KK18 pKa = 10.13NGGNPPVCTDD28 pKa = 3.22EE29 pKa = 4.01RR30 pKa = 11.84TKK32 pKa = 10.82YY33 pKa = 10.59IPVSCGNCIEE43 pKa = 4.2CRR45 pKa = 11.84KK46 pKa = 9.74KK47 pKa = 10.37KK48 pKa = 8.88QRR50 pKa = 11.84EE51 pKa = 3.67WRR53 pKa = 11.84VRR55 pKa = 11.84MLEE58 pKa = 4.12EE59 pKa = 4.1IKK61 pKa = 10.36HH62 pKa = 6.02DD63 pKa = 3.58KK64 pKa = 9.18TGKK67 pKa = 10.01FVTLTFSEE75 pKa = 4.42EE76 pKa = 3.74ALIEE80 pKa = 4.05LEE82 pKa = 4.11IEE84 pKa = 4.45AGTKK88 pKa = 8.47EE89 pKa = 4.37ANTVATLAVRR99 pKa = 11.84RR100 pKa = 11.84FLEE103 pKa = 4.1RR104 pKa = 11.84WRR106 pKa = 11.84KK107 pKa = 8.56EE108 pKa = 3.4YY109 pKa = 10.54RR110 pKa = 11.84KK111 pKa = 9.81SVKK114 pKa = 9.48HH115 pKa = 4.99WFITEE120 pKa = 4.24LGHH123 pKa = 7.29RR124 pKa = 11.84NTEE127 pKa = 3.82RR128 pKa = 11.84VHH130 pKa = 6.01IHH132 pKa = 6.73GIIFTDD138 pKa = 3.87KK139 pKa = 10.46EE140 pKa = 4.14KK141 pKa = 11.27EE142 pKa = 3.99DD143 pKa = 3.43IQRR146 pKa = 11.84IWKK149 pKa = 9.67YY150 pKa = 10.57GRR152 pKa = 11.84TDD154 pKa = 2.68TGYY157 pKa = 11.67SMGEE161 pKa = 3.65RR162 pKa = 11.84CVNYY166 pKa = 9.84VMKK169 pKa = 10.73YY170 pKa = 6.82ITKK173 pKa = 9.44TDD175 pKa = 3.38QDD177 pKa = 3.47HH178 pKa = 6.65KK179 pKa = 11.23GFVGKK184 pKa = 10.2ILTSPGIGRR193 pKa = 11.84GYY195 pKa = 9.59IGSYY199 pKa = 9.57NASKK203 pKa = 11.19NKK205 pKa = 9.52FNKK208 pKa = 9.78EE209 pKa = 4.18STNEE213 pKa = 4.01TYY215 pKa = 11.05KK216 pKa = 11.14LNNGCKK222 pKa = 9.26IALPQYY228 pKa = 9.31YY229 pKa = 9.54RR230 pKa = 11.84NKK232 pKa = 10.18IYY234 pKa = 10.51TEE236 pKa = 4.13EE237 pKa = 4.21EE238 pKa = 4.37KK239 pKa = 10.85EE240 pKa = 3.91QLWISKK246 pKa = 9.78IEE248 pKa = 3.97KK249 pKa = 9.68EE250 pKa = 4.16EE251 pKa = 4.46LYY253 pKa = 11.58VMGRR257 pKa = 11.84KK258 pKa = 8.67IRR260 pKa = 11.84NINTEE265 pKa = 3.69RR266 pKa = 11.84GQRR269 pKa = 11.84EE270 pKa = 4.08LKK272 pKa = 10.64YY273 pKa = 10.66AIEE276 pKa = 4.08YY277 pKa = 9.5AKK279 pKa = 10.48KK280 pKa = 9.8ISQQQGYY287 pKa = 8.5GAGEE291 pKa = 4.08GKK293 pKa = 9.71KK294 pKa = 10.61AYY296 pKa = 7.08MTRR299 pKa = 11.84KK300 pKa = 10.12NGTKK304 pKa = 9.28YY305 pKa = 10.88LEE307 pKa = 4.18VNRR310 pKa = 11.84RR311 pKa = 11.84IHH313 pKa = 6.71NIATMFEE320 pKa = 3.96EE321 pKa = 4.47SEE323 pKa = 4.32RR324 pKa = 11.84VAKK327 pKa = 10.6ARR329 pKa = 11.84IKK331 pKa = 10.05HH332 pKa = 5.1QNSINYY338 pKa = 8.23LNSMSYY344 pKa = 10.71EE345 pKa = 3.63EE346 pKa = 4.64SIRR349 pKa = 11.84NFSEE353 pKa = 3.57NEE355 pKa = 3.58HH356 pKa = 6.15RR357 pKa = 11.84VNSTGRR363 pKa = 11.84NIPAEE368 pKa = 3.95NEE370 pKa = 3.9GRR372 pKa = 11.84KK373 pKa = 9.82DD374 pKa = 3.45EE375 pKa = 4.62PGKK378 pKa = 9.36RR379 pKa = 11.84RR380 pKa = 11.84SYY382 pKa = 10.49RR383 pKa = 11.84GHH385 pKa = 6.43EE386 pKa = 3.96KK387 pKa = 10.79DD388 pKa = 3.35GG389 pKa = 3.74

Molecular weight: 45.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1685

68

696

280.8

31.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.3 ± 2.204

0.593 ± 0.321

4.688 ± 0.887

9.614 ± 1.99

2.611 ± 0.601

8.843 ± 0.778

1.543 ± 0.394

6.588 ± 0.425

7.596 ± 1.658

6.231 ± 0.536

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.73 ± 0.544

5.46 ± 0.686

2.671 ± 0.569

4.273 ± 0.729

5.697 ± 1.055

5.223 ± 0.328

7.774 ± 1.155

5.223 ± 0.595

1.602 ± 0.302

3.739 ± 0.817
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski