Salmonella phage vB_SenS_SB3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Jerseyvirus; unclassified Jerseyvirus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MLE2|A0A482MLE2_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS_SB3 OX=2562176 GN=SB3_059 PE=4 SV=1
MM1 pKa = 6.82ITKK4 pKa = 9.39EE5 pKa = 3.66QATYY9 pKa = 11.26LMKK12 pKa = 10.57LVHH15 pKa = 7.25DD16 pKa = 4.81IDD18 pKa = 4.81DD19 pKa = 4.3ASADD23 pKa = 3.34MAYY26 pKa = 9.6TIRR29 pKa = 11.84VDD31 pKa = 3.65DD32 pKa = 4.5EE33 pKa = 4.41EE34 pKa = 5.37YY35 pKa = 10.61EE36 pKa = 4.16AAAMRR41 pKa = 11.84WDD43 pKa = 3.31ACYY46 pKa = 10.54KK47 pKa = 10.66EE48 pKa = 5.05LMTFIASITEE58 pKa = 4.04TNEE61 pKa = 3.39

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MLY1|A0A482MLY1_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS_SB3 OX=2562176 GN=SB3_060 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.11PNYY5 pKa = 9.87LVTWTGRR12 pKa = 11.84NGEE15 pKa = 4.01IRR17 pKa = 11.84HH18 pKa = 5.78GKK20 pKa = 7.53VTSLQGIYY28 pKa = 10.75ARR30 pKa = 11.84VEE32 pKa = 3.33WWRR35 pKa = 11.84AHH37 pKa = 5.27AKK39 pKa = 9.2KK40 pKa = 9.96PRR42 pKa = 11.84YY43 pKa = 8.38FTMRR47 pKa = 11.84QDD49 pKa = 3.47KK50 pKa = 10.89LIVKK54 pKa = 9.6

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12681

29

859

201.3

22.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.849 ± 0.61

0.907 ± 0.115

6.127 ± 0.232

6.758 ± 0.407

3.738 ± 0.155

7.728 ± 0.302

1.656 ± 0.182

4.897 ± 0.183

5.97 ± 0.301

7.768 ± 0.315

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.166

4.274 ± 0.265

3.833 ± 0.212

3.738 ± 0.33

5.733 ± 0.198

5.899 ± 0.288

6.419 ± 0.324

7.342 ± 0.311

1.435 ± 0.145

3.383 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski