Poecilia formosa (Amazon molly) (Limia formosa)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei;

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30440 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087YD69|A0A087YD69_POEFO RAR related orphan receptor C OS=Poecilia formosa OX=48698 PE=3 SV=2
SS1 pKa = 7.36CPPFLQCQFNPCFPGVKK18 pKa = 9.57CVNTAPGFRR27 pKa = 11.84CDD29 pKa = 3.59ACPLGYY35 pKa = 10.54AGLPVEE41 pKa = 4.47GVGILFAQTNKK52 pKa = 9.79QVCDD56 pKa = 5.43DD57 pKa = 3.58IDD59 pKa = 3.69EE60 pKa = 4.9CKK62 pKa = 10.97GPDD65 pKa = 4.34NGGCTANSICHH76 pKa = 5.88NSVGSFYY83 pKa = 10.95CGSCKK88 pKa = 10.05TGFTGDD94 pKa = 3.44QVRR97 pKa = 11.84GCEE100 pKa = 4.19PEE102 pKa = 4.32LSCGNSLTNPCDD114 pKa = 3.35INAEE118 pKa = 4.42CIRR121 pKa = 11.84EE122 pKa = 3.91RR123 pKa = 11.84DD124 pKa = 3.67GSISCQCGIGWAGNGYY140 pKa = 9.95LCGKK144 pKa = 8.82DD145 pKa = 3.41TDD147 pKa = 3.57IDD149 pKa = 4.3GYY151 pKa = 10.17PDD153 pKa = 3.55EE154 pKa = 5.01KK155 pKa = 11.03LKK157 pKa = 11.4CKK159 pKa = 10.25DD160 pKa = 3.17ANCRR164 pKa = 11.84KK165 pKa = 9.73DD166 pKa = 3.26NCIFVPNSGQEE177 pKa = 3.85DD178 pKa = 4.13ADD180 pKa = 4.41RR181 pKa = 11.84DD182 pKa = 4.3GQGDD186 pKa = 3.88ACDD189 pKa = 4.9DD190 pKa = 4.28DD191 pKa = 6.95ADD193 pKa = 4.36GDD195 pKa = 4.52GIPNEE200 pKa = 4.16QDD202 pKa = 2.93NCRR205 pKa = 11.84LKK207 pKa = 11.13PNVDD211 pKa = 3.37QRR213 pKa = 11.84NSDD216 pKa = 3.65ADD218 pKa = 3.81SHH220 pKa = 7.54GDD222 pKa = 3.04ACDD225 pKa = 3.12NCRR228 pKa = 11.84VVNNPDD234 pKa = 3.29QRR236 pKa = 11.84DD237 pKa = 3.27TDD239 pKa = 3.86GDD241 pKa = 4.16GKK243 pKa = 11.42GDD245 pKa = 3.74ACDD248 pKa = 5.17DD249 pKa = 4.19DD250 pKa = 4.04MDD252 pKa = 6.26GDD254 pKa = 4.02GMIGVRR260 pKa = 11.84NFLDD264 pKa = 3.28NCQRR268 pKa = 11.84VQNRR272 pKa = 11.84DD273 pKa = 3.21QLDD276 pKa = 3.46RR277 pKa = 11.84DD278 pKa = 3.55GDD280 pKa = 4.42GVGDD284 pKa = 4.36ACDD287 pKa = 3.79SCPDD291 pKa = 3.6IPNPNQSDD299 pKa = 3.46VDD301 pKa = 3.63NDD303 pKa = 4.07LVGDD307 pKa = 4.11SCDD310 pKa = 3.63TNQDD314 pKa = 3.04SDD316 pKa = 4.1GDD318 pKa = 3.99GHH320 pKa = 7.29QDD322 pKa = 3.24TKK324 pKa = 11.65DD325 pKa = 3.4NCPLVINSSQLDD337 pKa = 3.61TDD339 pKa = 3.57KK340 pKa = 11.66DD341 pKa = 4.23GVGDD345 pKa = 4.08EE346 pKa = 5.46CDD348 pKa = 5.36DD349 pKa = 5.71DD350 pKa = 6.34DD351 pKa = 7.03DD352 pKa = 6.24NDD354 pKa = 5.44GIPDD358 pKa = 4.27TLPPGPDD365 pKa = 3.01NCRR368 pKa = 11.84LVPNPDD374 pKa = 3.79QIDD377 pKa = 3.91DD378 pKa = 4.08NNDD381 pKa = 3.08GVGDD385 pKa = 3.59ICEE388 pKa = 4.32SDD390 pKa = 3.29FDD392 pKa = 3.74QDD394 pKa = 4.05KK395 pKa = 11.41VIDD398 pKa = 5.09RR399 pKa = 11.84IDD401 pKa = 3.61NCPEE405 pKa = 3.68NAEE408 pKa = 4.12VTLTDD413 pKa = 3.81FRR415 pKa = 11.84AYY417 pKa = 8.19QTVVLDD423 pKa = 4.22PEE425 pKa = 5.32GDD427 pKa = 3.65AQIDD431 pKa = 4.13PNWVVLNQGMEE442 pKa = 4.11IVQTMNSDD450 pKa = 3.19PGLAVGYY457 pKa = 7.12TAFSGVDD464 pKa = 3.72FEE466 pKa = 5.24GTFHH470 pKa = 6.81VNTVTDD476 pKa = 3.57DD477 pKa = 4.08DD478 pKa = 4.35YY479 pKa = 12.0AGFIFGYY486 pKa = 9.58QDD488 pKa = 2.83SSSFYY493 pKa = 10.47VVMWKK498 pKa = 7.78QTEE501 pKa = 3.86QTYY504 pKa = 8.13WQATPFRR511 pKa = 11.84AVAEE515 pKa = 4.17PGIQLKK521 pKa = 10.07AVKK524 pKa = 9.92SRR526 pKa = 11.84SGPGEE531 pKa = 3.8HH532 pKa = 6.66LRR534 pKa = 11.84NSLWHH539 pKa = 6.47TGDD542 pKa = 3.39TNDD545 pKa = 4.93QVRR548 pKa = 11.84LLWKK552 pKa = 10.3DD553 pKa = 3.22PRR555 pKa = 11.84NVGWKK560 pKa = 10.39DD561 pKa = 3.16KK562 pKa = 11.21VSYY565 pKa = 10.03RR566 pKa = 11.84WYY568 pKa = 9.83LQHH571 pKa = 7.1RR572 pKa = 11.84PQIGYY577 pKa = 9.15IRR579 pKa = 11.84VRR581 pKa = 11.84FYY583 pKa = 11.37EE584 pKa = 4.3GTQLVADD591 pKa = 4.13SGVTIDD597 pKa = 3.09TTMRR601 pKa = 11.84GGRR604 pKa = 11.84LGVFCFSQEE613 pKa = 3.94NIIWSNLKK621 pKa = 9.68YY622 pKa = 10.31RR623 pKa = 11.84CNDD626 pKa = 4.18TIPEE630 pKa = 4.24DD631 pKa = 3.8FQEE634 pKa = 5.2FSAQHH639 pKa = 5.35TVDD642 pKa = 4.21SII644 pKa = 3.74

Molecular weight: 70.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087YRC7|A0A087YRC7_POEFO 5-hydroxytryptamine receptor 1B OS=Poecilia formosa OX=48698 GN=HTR1B PE=3 SV=2
GG1 pKa = 7.23GGPGRR6 pKa = 11.84PKK8 pKa = 10.4SGGPGRR14 pKa = 11.84PKK16 pKa = 10.24PGGGGGPGRR25 pKa = 11.84PKK27 pKa = 10.19PGGPGRR33 pKa = 11.84PKK35 pKa = 10.2PGGGGGPGRR44 pKa = 11.84PKK46 pKa = 10.3PGGGGGPGRR55 pKa = 11.84PKK57 pKa = 10.3PGGGGGPGRR66 pKa = 11.84PTT68 pKa = 3.21

Molecular weight: 5.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23408

7032

30440

17431430

27

27691

572.6

63.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.527 ± 0.012

2.295 ± 0.012

5.188 ± 0.009

6.868 ± 0.019

3.684 ± 0.01

6.279 ± 0.016

2.609 ± 0.008

4.328 ± 0.012

5.758 ± 0.017

9.552 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.294 ± 0.006

3.877 ± 0.008

5.714 ± 0.02

4.754 ± 0.014

5.681 ± 0.012

8.903 ± 0.02

5.562 ± 0.011

6.301 ± 0.016

1.148 ± 0.004

2.674 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski