Neolentinus lepideus HHB14362 ss-1

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Gloeophyllales; Gloeophyllaceae; Neolentinus; Neolentinus lepideus

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A165Q383|A0A165Q383_9AGAM Uncharacterized protein OS=Neolentinus lepideus HHB14362 ss-1 OX=1314782 GN=NEOLEDRAFT_1171731 PE=4 SV=1
MM1 pKa = 7.37SFCVDD6 pKa = 3.89PSTTFVDD13 pKa = 3.5QTLVSDD19 pKa = 3.83IQAPIFSTHH28 pKa = 5.83VDD30 pKa = 3.56CADD33 pKa = 3.48AGAAPSVHH41 pKa = 6.64IDD43 pKa = 3.58LAAFNTSAKK52 pKa = 8.94VNSMSHH58 pKa = 5.93LTTNGASEE66 pKa = 4.38NVSFPAPSLRR76 pKa = 11.84DD77 pKa = 3.5EE78 pKa = 4.51VFLPSTFTGGYY89 pKa = 6.88WRR91 pKa = 11.84SHH93 pKa = 4.46EE94 pKa = 4.28VGVCVDD100 pKa = 3.19GTEE103 pKa = 4.7RR104 pKa = 11.84SLEE107 pKa = 4.05DD108 pKa = 3.39LAAVASLGDD117 pKa = 3.7QPDD120 pKa = 3.52VAVFGDD126 pKa = 3.62ARR128 pKa = 11.84RR129 pKa = 11.84NGPMSCSAPVDD140 pKa = 3.34QLQQLEE146 pKa = 4.24QDD148 pKa = 4.14FRR150 pKa = 11.84AQPAINGCAPLGPDD164 pKa = 4.17AADD167 pKa = 3.42ASAPPSTPLLAMKK180 pKa = 10.11ALPAEE185 pKa = 4.6DD186 pKa = 4.47VVDD189 pKa = 3.96DD190 pKa = 4.68HH191 pKa = 7.26PSFVDD196 pKa = 4.13DD197 pKa = 4.3SQSSTWLSFEE207 pKa = 4.26HH208 pKa = 7.22ADD210 pKa = 4.27VDD212 pKa = 4.79LEE214 pKa = 4.6LPALLDD220 pKa = 3.78SCDD223 pKa = 3.53VPFNSPSPSYY233 pKa = 11.01NIGLFDD239 pKa = 4.64FDD241 pKa = 4.87YY242 pKa = 10.82PPSPSTASATSSFDD256 pKa = 3.61SPPPTPLLSSLDD268 pKa = 3.79ISCLEE273 pKa = 4.16CSQYY277 pKa = 11.37AQDD280 pKa = 3.43GHH282 pKa = 6.55AVCGTCGFTALLGMEE297 pKa = 4.94GGCGGSGVRR306 pKa = 11.84VVNAGEE312 pKa = 4.02DD313 pKa = 3.59LMDD316 pKa = 3.84GVEE319 pKa = 4.37DD320 pKa = 4.77SRR322 pKa = 11.84GGCCDD327 pKa = 4.07VIPGLQHH334 pKa = 5.91TWTSKK339 pKa = 9.87IPLDD343 pKa = 3.69GGYY346 pKa = 10.62KK347 pKa = 10.4FGQLPPQAFEE357 pKa = 3.78QDD359 pKa = 3.47EE360 pKa = 4.78VNTLGVDD367 pKa = 4.67FSAA370 pKa = 5.42

Molecular weight: 38.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A165NQK6|A0A165NQK6_9AGAM G-patch domain-containing protein OS=Neolentinus lepideus HHB14362 ss-1 OX=1314782 GN=NEOLEDRAFT_1182897 PE=4 SV=1
MM1 pKa = 7.54PRR3 pKa = 11.84ILKK6 pKa = 9.67QVAQLLARR14 pKa = 11.84PPALPHH20 pKa = 6.24ASSVVSTLTGLHH32 pKa = 6.2RR33 pKa = 11.84PSSQRR38 pKa = 11.84LTPLPFALAFQSSSLLTPSFVGRR61 pKa = 11.84SSALLQLNQVRR72 pKa = 11.84WGSRR76 pKa = 11.84GTEE79 pKa = 3.93YY80 pKa = 10.77QPSQRR85 pKa = 11.84VRR87 pKa = 11.84KK88 pKa = 9.28RR89 pKa = 11.84RR90 pKa = 11.84HH91 pKa = 4.87GFLARR96 pKa = 11.84KK97 pKa = 9.53KK98 pKa = 9.03SQHH101 pKa = 5.36GPKK104 pKa = 9.63ILARR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84AKK112 pKa = 9.85GRR114 pKa = 11.84KK115 pKa = 8.43FLSHH119 pKa = 6.98

Molecular weight: 13.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13106

2

13108

5153932

49

5057

393.2

43.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.431 ± 0.019

1.331 ± 0.009

5.557 ± 0.013

6.016 ± 0.021

3.597 ± 0.013

6.504 ± 0.021

2.525 ± 0.01

4.819 ± 0.016

4.535 ± 0.02

9.236 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.181 ± 0.008

3.301 ± 0.01

6.398 ± 0.025

3.681 ± 0.014

6.362 ± 0.021

8.743 ± 0.03

5.935 ± 0.015

6.554 ± 0.016

1.496 ± 0.009

2.799 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski