Hubei sobemo-like virus 36

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEM8|A0A1L3KEM8_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 36 OX=1923223 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84SLLFHH7 pKa = 6.79MKK9 pKa = 9.94RR10 pKa = 11.84HH11 pKa = 6.12IDD13 pKa = 3.47ISDD16 pKa = 3.51LAMDD20 pKa = 4.62LEE22 pKa = 4.52TRR24 pKa = 11.84TRR26 pKa = 11.84VVNKK30 pKa = 9.94ILLDD34 pKa = 3.87LKK36 pKa = 9.26PARR39 pKa = 11.84WEE41 pKa = 3.81IPEE44 pKa = 4.7DD45 pKa = 3.64FLSFSHH51 pKa = 7.55FYY53 pKa = 10.54RR54 pKa = 11.84VVKK57 pKa = 10.69DD58 pKa = 3.48LDD60 pKa = 3.99YY61 pKa = 10.52TSSPGYY67 pKa = 9.84PYY69 pKa = 10.13MRR71 pKa = 11.84TATNIRR77 pKa = 11.84DD78 pKa = 3.79FLKK81 pKa = 10.44VDD83 pKa = 3.69SSGEE87 pKa = 3.8PHH89 pKa = 7.01PEE91 pKa = 3.46KK92 pKa = 10.95LNYY95 pKa = 9.63LWSLVKK101 pKa = 10.75NQIATKK107 pKa = 9.74KK108 pKa = 9.91ADD110 pKa = 4.68PIRR113 pKa = 11.84MFIKK117 pKa = 10.37PEE119 pKa = 4.79PISLTKK125 pKa = 10.84VKK127 pKa = 10.38DD128 pKa = 3.09KK129 pKa = 10.52RR130 pKa = 11.84YY131 pKa = 9.71RR132 pKa = 11.84IISAVSLVDD141 pKa = 3.76QIIDD145 pKa = 3.2HH146 pKa = 6.14MLFANFNHH154 pKa = 6.78KK155 pKa = 10.22LVEE158 pKa = 4.23NCQRR162 pKa = 11.84TPVKK166 pKa = 10.06TGWTPIQGGWKK177 pKa = 9.03VVPRR181 pKa = 11.84LGMVSTDD188 pKa = 2.93KK189 pKa = 11.4SSFDD193 pKa = 3.12WTVRR197 pKa = 11.84PWMLDD202 pKa = 3.07VEE204 pKa = 4.6LEE206 pKa = 3.83IRR208 pKa = 11.84TRR210 pKa = 11.84LCNNVTEE217 pKa = 4.78EE218 pKa = 4.1WKK220 pKa = 10.93QMAKK224 pKa = 8.57WRR226 pKa = 11.84YY227 pKa = 7.42EE228 pKa = 3.74QLFNRR233 pKa = 11.84PLFINSAGIVFRR245 pKa = 11.84SNISGIMKK253 pKa = 9.79SGCVNTISTNSIIQLIIHH271 pKa = 5.79YY272 pKa = 9.73RR273 pKa = 11.84VSAEE277 pKa = 3.82LNQPFPYY284 pKa = 9.2IWAMGDD290 pKa = 3.63DD291 pKa = 4.9VIQEE295 pKa = 4.52DD296 pKa = 3.82SGSDD300 pKa = 3.52YY301 pKa = 9.69FTHH304 pKa = 7.26LEE306 pKa = 4.19RR307 pKa = 11.84YY308 pKa = 9.72CILKK312 pKa = 10.49EE313 pKa = 3.96KK314 pKa = 6.88TTHH317 pKa = 6.14VEE319 pKa = 3.88FAGYY323 pKa = 9.92RR324 pKa = 11.84YY325 pKa = 10.12NGYY328 pKa = 9.4NVEE331 pKa = 3.86PLYY334 pKa = 10.49FGKK337 pKa = 9.87HH338 pKa = 5.46AYY340 pKa = 9.71VLLHH344 pKa = 6.39QKK346 pKa = 9.82LQYY349 pKa = 8.95ITEE352 pKa = 4.23TANAYY357 pKa = 10.13ALLYY361 pKa = 10.1HH362 pKa = 6.65RR363 pKa = 11.84SKK365 pKa = 10.65RR366 pKa = 11.84ASKK369 pKa = 9.91IKK371 pKa = 9.93TYY373 pKa = 10.6LRR375 pKa = 11.84QLNPEE380 pKa = 5.08LISDD384 pKa = 5.03DD385 pKa = 4.15VLDD388 pKa = 4.98DD389 pKa = 3.69LWDD392 pKa = 3.94NEE394 pKa = 4.5SS395 pKa = 3.18

Molecular weight: 46.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEM8|A0A1L3KEM8_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 36 OX=1923223 PE=4 SV=1
MM1 pKa = 7.74PLHH4 pKa = 7.38VYY6 pKa = 9.72RR7 pKa = 11.84QAVPDD12 pKa = 3.57ILIGKK17 pKa = 8.11NGSYY21 pKa = 10.72FEE23 pKa = 4.42ISNAPMIEE31 pKa = 4.07SDD33 pKa = 4.46SVTDD37 pKa = 3.7LCYY40 pKa = 10.44VTVKK44 pKa = 10.3PQIFSQLKK52 pKa = 7.73TSIARR57 pKa = 11.84FPKK60 pKa = 10.19GMVEE64 pKa = 4.2GFVSAVGRR72 pKa = 11.84SGASQGSLEE81 pKa = 4.2KK82 pKa = 10.37AASRR86 pKa = 11.84GIVIYY91 pKa = 10.78NGSTKK96 pKa = 10.12PGYY99 pKa = 10.3SGAAYY104 pKa = 10.52LIGNQIYY111 pKa = 10.38GMHH114 pKa = 6.92IGHH117 pKa = 6.65SSGANVGISHH127 pKa = 7.06VVLQYY132 pKa = 11.49DD133 pKa = 3.73LNKK136 pKa = 10.1YY137 pKa = 9.1KK138 pKa = 11.1YY139 pKa = 9.44HH140 pKa = 5.12VTVDD144 pKa = 3.43KK145 pKa = 10.66EE146 pKa = 4.4GSVTFSSPGMMGSKK160 pKa = 10.48EE161 pKa = 3.82EE162 pKa = 4.25DD163 pKa = 3.01LTRR166 pKa = 11.84VYY168 pKa = 10.68DD169 pKa = 4.34GKK171 pKa = 10.16WSADD175 pKa = 2.93EE176 pKa = 4.04FLRR179 pKa = 11.84EE180 pKa = 3.92RR181 pKa = 11.84KK182 pKa = 9.3GLKK185 pKa = 8.95SWNDD189 pKa = 2.71IKK191 pKa = 11.33LDD193 pKa = 5.12DD194 pKa = 4.03KK195 pKa = 11.62ALWANEE201 pKa = 3.89FEE203 pKa = 4.56STLRR207 pKa = 11.84SLPPDD212 pKa = 3.25VLKK215 pKa = 10.83FVVDD219 pKa = 3.68RR220 pKa = 11.84AGAEE224 pKa = 4.1LQKK227 pKa = 11.43SNITVQGQSDD237 pKa = 4.06DD238 pKa = 4.55EE239 pKa = 4.68IPVDD243 pKa = 2.92IHH245 pKa = 7.58IYY247 pKa = 10.37SPQQQEE253 pKa = 4.32SLSQRR258 pKa = 11.84VTNLEE263 pKa = 3.71ARR265 pKa = 11.84VSKK268 pKa = 10.97LEE270 pKa = 3.88QKK272 pKa = 10.13KK273 pKa = 10.26APKK276 pKa = 9.53QGPVQKK282 pKa = 9.69VTVFKK287 pKa = 10.9CLQCDD292 pKa = 3.68STSTTRR298 pKa = 11.84LAVANHH304 pKa = 7.25MITKK308 pKa = 9.18HH309 pKa = 4.5QVPEE313 pKa = 3.91EE314 pKa = 4.06SLDD317 pKa = 3.67LGAILAMEE325 pKa = 4.66KK326 pKa = 9.91EE327 pKa = 4.48VKK329 pKa = 10.25PEE331 pKa = 3.81GVLSSDD337 pKa = 3.56EE338 pKa = 4.18SDD340 pKa = 3.33LVGNGKK346 pKa = 8.0VFRR349 pKa = 11.84NRR351 pKa = 11.84SLSPPKK357 pKa = 10.08KK358 pKa = 9.8SSNSKK363 pKa = 10.21SSSSPSRR370 pKa = 11.84KK371 pKa = 9.14PNRR374 pKa = 11.84FQQSAQNQKK383 pKa = 10.6NMEE386 pKa = 4.17NLMNKK391 pKa = 9.95LNEE394 pKa = 4.11NLLKK398 pKa = 10.63LVQNLPGPSSAQGQKK413 pKa = 10.47

Molecular weight: 45.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

808

395

413

404.0

45.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.95 ± 0.437

0.866 ± 0.099

5.569 ± 0.514

5.569 ± 0.171

3.342 ± 0.479

5.446 ± 1.285

2.475 ± 0.38

6.064 ± 1.035

7.673 ± 0.566

8.911 ± 0.48

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.126

5.198 ± 0.091

4.827 ± 0.182

4.579 ± 1.042

5.074 ± 0.848

9.53 ± 1.65

4.579 ± 0.67

7.178 ± 0.574

1.609 ± 0.624

3.96 ± 0.746
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski