Merismopedia glauca CCAP 1448/3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Merismopediaceae; Merismopedia; Merismopedia glauca

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4638 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T1C4B0|A0A2T1C4B0_9CYAN TonB_C domain-containing protein OS=Merismopedia glauca CCAP 1448/3 OX=1296344 GN=C7B64_10150 PE=4 SV=1
MM1 pKa = 8.14DD2 pKa = 5.01DD3 pKa = 3.05WSKK6 pKa = 11.16EE7 pKa = 3.59FWQAIDD13 pKa = 3.86DD14 pKa = 5.13AILAVDD20 pKa = 3.99RR21 pKa = 11.84VFTGVATTLEE31 pKa = 4.28TAVDD35 pKa = 3.94DD36 pKa = 4.9LLMEE40 pKa = 4.15VATNVEE46 pKa = 4.26STVADD51 pKa = 3.53VQKK54 pKa = 10.69IIVSEE59 pKa = 3.9LDD61 pKa = 3.33EE62 pKa = 5.01NVPEE66 pKa = 3.93WRR68 pKa = 11.84EE69 pKa = 3.85WLDD72 pKa = 3.53GDD74 pKa = 5.79DD75 pKa = 6.07DD76 pKa = 5.07LFPDD80 pKa = 3.59MEE82 pKa = 4.62EE83 pKa = 4.54FNHH86 pKa = 5.72HH87 pKa = 5.06TEE89 pKa = 4.49FYY91 pKa = 10.61DD92 pKa = 3.57VYY94 pKa = 10.53PVFPSAEE101 pKa = 4.08NNPACIGCRR110 pKa = 11.84NYY112 pKa = 10.38HH113 pKa = 4.62GQVYY117 pKa = 10.22NGNILVCGMHH127 pKa = 6.3PSGWEE132 pKa = 3.98DD133 pKa = 3.48EE134 pKa = 4.65KK135 pKa = 11.71CPDD138 pKa = 3.36WEE140 pKa = 4.84SNN142 pKa = 3.31

Molecular weight: 16.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T1C8W3|A0A2T1C8W3_9CYAN Uncharacterized protein OS=Merismopedia glauca CCAP 1448/3 OX=1296344 GN=C7B64_02525 PE=4 SV=1
MM1 pKa = 7.29TRR3 pKa = 11.84RR4 pKa = 11.84TLEE7 pKa = 3.73GTNRR11 pKa = 11.84KK12 pKa = 7.62QKK14 pKa = 8.83RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84NGRR29 pKa = 11.84KK30 pKa = 8.47VIKK33 pKa = 10.05ARR35 pKa = 11.84RR36 pKa = 11.84VKK38 pKa = 10.19GRR40 pKa = 11.84YY41 pKa = 9.18RR42 pKa = 11.84LTVV45 pKa = 2.95

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4638

0

4638

1433816

25

3642

309.1

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.995 ± 0.035

1.022 ± 0.013

4.882 ± 0.025

6.28 ± 0.036

3.834 ± 0.023

6.643 ± 0.041

1.822 ± 0.018

6.881 ± 0.026

4.948 ± 0.03

11.057 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.789 ± 0.016

4.27 ± 0.029

4.787 ± 0.027

5.3 ± 0.03

5.099 ± 0.029

6.702 ± 0.029

5.622 ± 0.028

6.497 ± 0.027

1.485 ± 0.016

3.086 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski