Amino acid dipepetide frequency for Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.845AlaAla: 5.845 ± 0.642
1.525AlaCys: 1.525 ± 0.723
7.116AlaAsp: 7.116 ± 1.388
3.558AlaGlu: 3.558 ± 1.027
0.0AlaPhe: 0.0 ± 0.0
2.795AlaGly: 2.795 ± 0.269
2.795AlaHis: 2.795 ± 0.296
2.287AlaIle: 2.287 ± 0.827
5.845AlaLys: 5.845 ± 0.972
2.541AlaLeu: 2.541 ± 0.274
0.762AlaMet: 0.762 ± 0.298
1.779AlaAsn: 1.779 ± 0.584
6.099AlaPro: 6.099 ± 1.051
2.541AlaGln: 2.541 ± 0.967
2.033AlaArg: 2.033 ± 0.658
7.37AlaSer: 7.37 ± 0.721
5.845AlaThr: 5.845 ± 0.748
2.795AlaVal: 2.795 ± 0.303
0.508AlaTrp: 0.508 ± 0.296
0.762AlaTyr: 0.762 ± 0.313
0.0AlaXaa: 0.0 ± 0.0
Cys
1.017CysAla: 1.017 ± 0.391
0.0CysCys: 0.0 ± 0.0
1.271CysAsp: 1.271 ± 0.295
0.508CysGlu: 0.508 ± 0.295
1.017CysPhe: 1.017 ± 0.59
0.0CysGly: 0.0 ± 0.0
1.017CysHis: 1.017 ± 0.35
0.508CysIle: 0.508 ± 0.295
1.017CysLys: 1.017 ± 0.391
2.795CysLeu: 2.795 ± 0.324
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.254CysPro: 0.254 ± 0.284
0.0CysGln: 0.0 ± 0.0
0.508CysArg: 0.508 ± 0.295
0.508CysSer: 0.508 ± 0.295
0.508CysThr: 0.508 ± 0.295
2.033CysVal: 2.033 ± 0.782
0.0CysTrp: 0.0 ± 0.0
1.779CysTyr: 1.779 ± 0.301
0.0CysXaa: 0.0 ± 0.0
Asp
4.32AspAla: 4.32 ± 0.84
2.033AspCys: 2.033 ± 0.845
1.525AspAsp: 1.525 ± 0.388
2.033AspGlu: 2.033 ± 0.432
4.066AspPhe: 4.066 ± 0.478
4.066AspGly: 4.066 ± 0.479
2.033AspHis: 2.033 ± 0.782
3.558AspIle: 3.558 ± 0.4
2.541AspLys: 2.541 ± 0.632
2.033AspLeu: 2.033 ± 0.934
0.508AspMet: 0.508 ± 0.296
3.558AspAsn: 3.558 ± 0.67
2.795AspPro: 2.795 ± 0.648
3.558AspGln: 3.558 ± 0.4
4.574AspArg: 4.574 ± 0.453
6.099AspSer: 6.099 ± 1.521
1.525AspThr: 1.525 ± 0.625
3.05AspVal: 3.05 ± 1.19
1.017AspTrp: 1.017 ± 0.431
1.017AspTyr: 1.017 ± 0.391
0.0AspXaa: 0.0 ± 0.0
Glu
2.795GluAla: 2.795 ± 0.861
1.017GluCys: 1.017 ± 0.467
3.304GluAsp: 3.304 ± 0.435
3.558GluGlu: 3.558 ± 0.67
1.271GluPhe: 1.271 ± 0.316
2.033GluGly: 2.033 ± 0.197
2.795GluHis: 2.795 ± 0.557
3.05GluIle: 3.05 ± 0.661
5.337GluLys: 5.337 ± 0.829
5.083GluLeu: 5.083 ± 1.294
0.0GluMet: 0.0 ± 0.0
2.287GluAsn: 2.287 ± 0.344
4.066GluPro: 4.066 ± 0.703
2.795GluGln: 2.795 ± 0.789
1.525GluArg: 1.525 ± 0.497
3.304GluSer: 3.304 ± 1.153
0.762GluThr: 0.762 ± 0.403
2.795GluVal: 2.795 ± 0.545
2.033GluTrp: 2.033 ± 0.454
2.287GluTyr: 2.287 ± 0.893
0.0GluXaa: 0.0 ± 0.0
Phe
1.271PheAla: 1.271 ± 0.22
0.0PheCys: 0.0 ± 0.0
0.762PheAsp: 0.762 ± 0.298
3.812PheGlu: 3.812 ± 0.866
2.287PhePhe: 2.287 ± 0.344
6.099PheGly: 6.099 ± 0.538
3.05PheHis: 3.05 ± 0.661
0.762PheIle: 0.762 ± 0.298
2.033PheLys: 2.033 ± 0.265
2.033PheLeu: 2.033 ± 0.86
0.762PheMet: 0.762 ± 0.298
3.812PheAsn: 3.812 ± 0.336
4.574PhePro: 4.574 ± 0.529
0.508PheGln: 0.508 ± 0.425
2.033PheArg: 2.033 ± 0.265
3.05PheSer: 3.05 ± 1.19
2.795PheThr: 2.795 ± 0.787
1.017PheVal: 1.017 ± 0.591
0.0PheTrp: 0.0 ± 0.0
3.304PheTyr: 3.304 ± 0.702
0.0PheXaa: 0.0 ± 0.0
Gly
5.337GlyAla: 5.337 ± 0.963
1.779GlyCys: 1.779 ± 0.2
1.779GlyAsp: 1.779 ± 0.641
0.762GlyGlu: 0.762 ± 0.302
2.033GlyPhe: 2.033 ± 0.432
4.32GlyGly: 4.32 ± 0.83
1.779GlyHis: 1.779 ± 0.378
2.795GlyIle: 2.795 ± 0.937
7.624GlyLys: 7.624 ± 1.519
2.033GlyLeu: 2.033 ± 1.017
1.271GlyMet: 1.271 ± 0.295
4.828GlyAsn: 4.828 ± 0.757
4.066GlyPro: 4.066 ± 0.749
3.812GlyGln: 3.812 ± 0.729
2.287GlyArg: 2.287 ± 0.542
4.828GlySer: 4.828 ± 1.166
3.304GlyThr: 3.304 ± 1.307
3.558GlyVal: 3.558 ± 0.997
2.287GlyTrp: 2.287 ± 0.827
3.812GlyTyr: 3.812 ± 0.801
0.0GlyXaa: 0.0 ± 0.0
His
0.254HisAla: 0.254 ± 0.213
1.525HisCys: 1.525 ± 0.595
1.271HisAsp: 1.271 ± 0.316
1.017HisGlu: 1.017 ± 0.391
1.525HisPhe: 1.525 ± 0.595
0.762HisGly: 0.762 ± 0.387
0.762HisHis: 0.762 ± 0.387
0.508HisIle: 0.508 ± 0.295
1.017HisLys: 1.017 ± 0.431
2.541HisLeu: 2.541 ± 0.44
0.508HisMet: 0.508 ± 0.296
0.0HisAsn: 0.0 ± 0.0
1.525HisPro: 1.525 ± 0.595
2.287HisGln: 2.287 ± 0.511
0.0HisArg: 0.0 ± 0.0
2.795HisSer: 2.795 ± 0.628
1.779HisThr: 1.779 ± 0.2
2.795HisVal: 2.795 ± 1.033
0.0HisTrp: 0.0 ± 0.0
0.762HisTyr: 0.762 ± 0.298
0.0HisXaa: 0.0 ± 0.0
Ile
3.05IleAla: 3.05 ± 0.569
0.508IleCys: 0.508 ± 0.295
2.795IleAsp: 2.795 ± 0.545
2.541IleGlu: 2.541 ± 0.44
1.271IlePhe: 1.271 ± 0.295
1.017IleGly: 1.017 ± 0.35
0.508IleHis: 0.508 ± 0.295
0.0IleIle: 0.0 ± 0.0
2.541IleLys: 2.541 ± 0.378
3.05IleLeu: 3.05 ± 0.569
1.525IleMet: 1.525 ± 0.589
1.779IleAsn: 1.779 ± 0.2
1.779IlePro: 1.779 ± 0.516
3.05IleGln: 3.05 ± 0.234
3.05IleArg: 3.05 ± 0.477
4.32IleSer: 4.32 ± 1.084
4.066IleThr: 4.066 ± 0.799
1.525IleVal: 1.525 ± 0.599
2.795IleTrp: 2.795 ± 0.545
1.525IleTyr: 1.525 ± 0.599
0.0IleXaa: 0.0 ± 0.0
Lys
3.812LysAla: 3.812 ± 0.886
1.525LysCys: 1.525 ± 0.589
4.066LysAsp: 4.066 ± 0.733
2.795LysGlu: 2.795 ± 0.51
3.812LysPhe: 3.812 ± 0.374
2.795LysGly: 2.795 ± 0.861
0.762LysHis: 0.762 ± 0.298
2.795LysIle: 2.795 ± 0.545
4.32LysLys: 4.32 ± 1.079
2.541LysLeu: 2.541 ± 0.499
2.033LysMet: 2.033 ± 0.782
0.762LysAsn: 0.762 ± 0.298
0.508LysPro: 0.508 ± 0.425
3.558LysGln: 3.558 ± 0.4
6.607LysArg: 6.607 ± 0.461
2.541LysSer: 2.541 ± 0.378
5.337LysThr: 5.337 ± 0.848
5.337LysVal: 5.337 ± 1.439
0.0LysTrp: 0.0 ± 0.0
1.271LysTyr: 1.271 ± 0.611
0.0LysXaa: 0.0 ± 0.0
Leu
0.762LeuAla: 0.762 ± 0.476
0.508LeuCys: 0.508 ± 0.295
4.32LeuAsp: 4.32 ± 0.7
3.05LeuGlu: 3.05 ± 0.558
2.795LeuPhe: 2.795 ± 0.517
5.337LeuGly: 5.337 ± 0.65
0.0LeuHis: 0.0 ± 0.0
4.32LeuIle: 4.32 ± 0.793
1.525LeuLys: 1.525 ± 0.807
0.762LeuLeu: 0.762 ± 0.403
3.05LeuMet: 3.05 ± 0.587
4.828LeuAsn: 4.828 ± 0.506
6.861LeuPro: 6.861 ± 1.1
5.337LeuGln: 5.337 ± 0.458
0.762LeuArg: 0.762 ± 0.298
2.287LeuSer: 2.287 ± 0.46
7.37LeuThr: 7.37 ± 1.275
6.099LeuVal: 6.099 ± 1.228
0.0LeuTrp: 0.0 ± 0.0
1.525LeuTyr: 1.525 ± 0.595
0.0LeuXaa: 0.0 ± 0.0
Met
2.795MetAla: 2.795 ± 1.088
1.017MetCys: 1.017 ± 0.391
0.762MetAsp: 0.762 ± 0.403
1.779MetGlu: 1.779 ± 0.655
1.017MetPhe: 1.017 ± 0.391
2.033MetGly: 2.033 ± 0.514
0.508MetHis: 0.508 ± 0.296
0.762MetIle: 0.762 ± 0.298
0.0MetLys: 0.0 ± 0.0
1.271MetLeu: 1.271 ± 0.295
0.0MetMet: 0.0 ± 0.0
1.779MetAsn: 1.779 ± 0.51
0.508MetPro: 0.508 ± 0.296
0.0MetGln: 0.0 ± 0.0
0.0MetArg: 0.0 ± 0.0
1.017MetSer: 1.017 ± 0.391
1.271MetThr: 1.271 ± 0.479
3.05MetVal: 3.05 ± 0.234
0.0MetTrp: 0.0 ± 0.0
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
2.033AsnAla: 2.033 ± 0.782
0.0AsnCys: 0.0 ± 0.0
2.541AsnAsp: 2.541 ± 0.323
2.033AsnGlu: 2.033 ± 0.197
3.05AsnPhe: 3.05 ± 0.491
4.066AsnGly: 4.066 ± 0.479
1.779AsnHis: 1.779 ± 0.709
2.287AsnIle: 2.287 ± 0.382
2.541AsnLys: 2.541 ± 0.524
4.066AsnLeu: 4.066 ± 0.612
1.525AsnMet: 1.525 ± 0.599
6.861AsnAsn: 6.861 ± 2.678
4.574AsnPro: 4.574 ± 1.388
1.271AsnGln: 1.271 ± 0.525
2.541AsnArg: 2.541 ± 0.323
5.337AsnSer: 5.337 ± 0.471
4.828AsnThr: 4.828 ± 0.546
2.033AsnVal: 2.033 ± 0.514
4.32AsnTrp: 4.32 ± 0.408
2.287AsnTyr: 2.287 ± 0.344
0.0AsnXaa: 0.0 ± 0.0
Pro
6.353ProAla: 6.353 ± 0.815
1.525ProCys: 1.525 ± 0.322
3.304ProAsp: 3.304 ± 0.6
2.033ProGlu: 2.033 ± 0.454
3.812ProPhe: 3.812 ± 0.336
4.574ProGly: 4.574 ± 0.973
1.017ProHis: 1.017 ± 0.35
1.779ProIle: 1.779 ± 0.584
2.541ProLys: 2.541 ± 0.274
4.32ProLeu: 4.32 ± 0.451
1.017ProMet: 1.017 ± 0.345
1.525ProAsn: 1.525 ± 0.887
4.828ProPro: 4.828 ± 1.359
3.812ProGln: 3.812 ± 0.767
2.287ProArg: 2.287 ± 0.894
6.353ProSer: 6.353 ± 0.44
2.795ProThr: 2.795 ± 0.573
6.607ProVal: 6.607 ± 0.774
0.762ProTrp: 0.762 ± 0.298
1.017ProTyr: 1.017 ± 0.431
0.0ProXaa: 0.0 ± 0.0
Gln
4.32GlnAla: 4.32 ± 0.391
0.508GlnCys: 0.508 ± 0.295
1.779GlnAsp: 1.779 ± 0.2
3.05GlnGlu: 3.05 ± 0.895
1.271GlnPhe: 1.271 ± 0.22
3.05GlnGly: 3.05 ± 1.19
0.508GlnHis: 0.508 ± 0.296
4.066GlnIle: 4.066 ± 0.478
2.795GlnLys: 2.795 ± 0.653
1.017GlnLeu: 1.017 ± 0.431
1.271GlnMet: 1.271 ± 0.311
2.795GlnAsn: 2.795 ± 0.727
5.845GlnPro: 5.845 ± 1.073
3.812GlnGln: 3.812 ± 0.554
3.304GlnArg: 3.304 ± 0.618
3.812GlnSer: 3.812 ± 1.488
1.779GlnThr: 1.779 ± 0.808
2.287GlnVal: 2.287 ± 0.893
2.033GlnTrp: 2.033 ± 0.571
5.591GlnTyr: 5.591 ± 0.875
0.0GlnXaa: 0.0 ± 0.0
Arg
0.508ArgAla: 0.508 ± 0.343
0.254ArgCys: 0.254 ± 0.284
3.812ArgAsp: 3.812 ± 0.939
1.779ArgGlu: 1.779 ± 0.655
1.525ArgPhe: 1.525 ± 0.277
2.033ArgGly: 2.033 ± 0.366
0.762ArgHis: 0.762 ± 0.313
1.779ArgIle: 1.779 ± 0.655
1.017ArgLys: 1.017 ± 0.283
6.099ArgLeu: 6.099 ± 0.738
1.779ArgMet: 1.779 ± 0.637
3.05ArgAsn: 3.05 ± 0.234
3.05ArgPro: 3.05 ± 0.491
2.541ArgGln: 2.541 ± 0.765
2.795ArgArg: 2.795 ± 1.524
3.05ArgSer: 3.05 ± 0.821
3.558ArgThr: 3.558 ± 1.168
4.32ArgVal: 4.32 ± 0.374
2.033ArgTrp: 2.033 ± 0.265
1.779ArgTyr: 1.779 ± 0.301
0.0ArgXaa: 0.0 ± 0.0
Ser
5.591SerAla: 5.591 ± 0.424
0.0SerCys: 0.0 ± 0.0
5.337SerAsp: 5.337 ± 1.517
5.845SerGlu: 5.845 ± 0.623
3.304SerPhe: 3.304 ± 0.253
5.083SerGly: 5.083 ± 1.998
1.017SerHis: 1.017 ± 0.35
1.017SerIle: 1.017 ± 0.391
3.812SerLys: 3.812 ± 0.391
3.558SerLeu: 3.558 ± 0.604
0.762SerMet: 0.762 ± 0.403
5.083SerAsn: 5.083 ± 0.868
2.541SerPro: 2.541 ± 0.824
6.607SerGln: 6.607 ± 0.571
4.828SerArg: 4.828 ± 0.748
7.878SerSer: 7.878 ± 1.707
8.895SerThr: 8.895 ± 1.739
5.337SerVal: 5.337 ± 0.542
1.017SerTrp: 1.017 ± 0.35
3.05SerTyr: 3.05 ± 0.234
0.0SerXaa: 0.0 ± 0.0
Thr
4.066ThrAla: 4.066 ± 0.733
0.0ThrCys: 0.0 ± 0.0
2.541ThrAsp: 2.541 ± 0.859
2.287ThrGlu: 2.287 ± 0.625
3.812ThrPhe: 3.812 ± 0.617
6.099ThrGly: 6.099 ± 0.944
1.017ThrHis: 1.017 ± 0.467
2.287ThrIle: 2.287 ± 0.511
4.32ThrLys: 4.32 ± 1.084
2.033ThrLeu: 2.033 ± 0.553
0.508ThrMet: 0.508 ± 0.263
8.132ThrAsn: 8.132 ± 0.497
3.558ThrPro: 3.558 ± 0.531
4.574ThrGln: 4.574 ± 0.821
4.32ThrArg: 4.32 ± 0.439
8.64ThrSer: 8.64 ± 1.639
9.657ThrThr: 9.657 ± 1.769
3.304ThrVal: 3.304 ± 0.182
1.525ThrTrp: 1.525 ± 0.595
0.762ThrTyr: 0.762 ± 0.298
0.0ThrXaa: 0.0 ± 0.0
Val
3.558ValAla: 3.558 ± 0.902
0.0ValCys: 0.0 ± 0.0
6.353ValAsp: 6.353 ± 0.779
6.607ValGlu: 6.607 ± 1.069
2.795ValPhe: 2.795 ± 0.394
4.066ValGly: 4.066 ± 0.534
0.0ValHis: 0.0 ± 0.0
5.083ValIle: 5.083 ± 1.294
4.066ValLys: 4.066 ± 0.758
3.812ValLeu: 3.812 ± 0.841
0.762ValMet: 0.762 ± 0.298
4.574ValAsn: 4.574 ± 0.411
4.828ValPro: 4.828 ± 0.516
1.271ValGln: 1.271 ± 0.22
2.287ValArg: 2.287 ± 0.827
4.32ValSer: 4.32 ± 0.612
3.558ValThr: 3.558 ± 0.717
3.05ValVal: 3.05 ± 1.131
1.017ValTrp: 1.017 ± 0.35
1.525ValTyr: 1.525 ± 0.595
0.0ValXaa: 0.0 ± 0.0
Trp
4.32TrpAla: 4.32 ± 0.451
0.254TrpCys: 0.254 ± 0.284
0.0TrpAsp: 0.0 ± 0.0
2.541TrpGlu: 2.541 ± 0.378
0.508TrpPhe: 0.508 ± 0.296
1.525TrpGly: 1.525 ± 0.595
0.762TrpHis: 0.762 ± 0.298
1.017TrpIle: 1.017 ± 0.391
0.254TrpLys: 0.254 ± 0.213
3.304TrpLeu: 3.304 ± 0.464
0.762TrpMet: 0.762 ± 0.298
0.762TrpAsn: 0.762 ± 0.298
0.0TrpPro: 0.0 ± 0.0
1.779TrpGln: 1.779 ± 0.584
0.508TrpArg: 0.508 ± 0.296
0.0TrpSer: 0.0 ± 0.0
2.287TrpThr: 2.287 ± 0.344
0.762TrpVal: 0.762 ± 0.403
1.271TrpTrp: 1.271 ± 0.316
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.795TyrAla: 2.795 ± 0.545
0.762TyrCys: 0.762 ± 0.298
1.271TyrAsp: 1.271 ± 0.316
0.508TyrGlu: 0.508 ± 0.296
2.795TyrPhe: 2.795 ± 0.708
2.541TyrGly: 2.541 ± 0.323
0.762TyrHis: 0.762 ± 0.298
2.033TyrIle: 2.033 ± 0.772
2.033TyrLys: 2.033 ± 0.197
6.861TyrLeu: 6.861 ± 1.058
0.508TyrMet: 0.508 ± 0.296
1.779TyrAsn: 1.779 ± 0.709
0.0TyrPro: 0.0 ± 0.0
1.779TyrGln: 1.779 ± 0.51
1.271TyrArg: 1.271 ± 0.22
3.05TyrSer: 3.05 ± 1.19
1.525TyrThr: 1.525 ± 0.595
1.779TyrVal: 1.779 ± 0.2
0.0TyrTrp: 0.0 ± 0.0
0.762TyrTyr: 0.762 ± 0.298
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8 proteins (3936 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski