Cellulomonas sp. HLT2-17

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; unclassified Cellulomonas

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3785 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q5N5T6|A0A4Q5N5T6_9CELL Efflux RND transporter periplasmic adaptor subunit OS=Cellulomonas sp. HLT2-17 OX=1259133 GN=EUA98_07850 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.33RR3 pKa = 11.84PLVVLTATLAAAGMLAACSGGTTTPKK29 pKa = 10.73GADD32 pKa = 3.22GTAASGPIKK41 pKa = 9.89IWYY44 pKa = 8.93SNNPNEE50 pKa = 4.46VAWGEE55 pKa = 4.17QVVEE59 pKa = 4.18AWNADD64 pKa = 3.54HH65 pKa = 7.65ADD67 pKa = 3.77EE68 pKa = 4.37QVTGQEE74 pKa = 3.89IPAGRR79 pKa = 11.84SSEE82 pKa = 4.19EE83 pKa = 3.91VIGAAITAGNTPCLIYY99 pKa = 9.95NTAPVAVPGFQKK111 pKa = 10.58QGGLVDD117 pKa = 5.48LSTFPDD123 pKa = 3.64GAEE126 pKa = 4.01YY127 pKa = 10.35IEE129 pKa = 4.54ARR131 pKa = 11.84TGEE134 pKa = 4.13AASQYY139 pKa = 10.68VSADD143 pKa = 2.6GDD145 pKa = 4.33YY146 pKa = 11.5YY147 pKa = 11.01QMPWKK152 pKa = 10.03SNPVMFFYY160 pKa = 11.15NKK162 pKa = 10.62AIMTAAGVDD171 pKa = 3.9PEE173 pKa = 4.32NPPLATYY180 pKa = 10.84DD181 pKa = 3.64EE182 pKa = 4.76FLDD185 pKa = 3.71TSRR188 pKa = 11.84KK189 pKa = 9.06IVASGAATYY198 pKa = 10.24AIYY201 pKa = 9.43PSPASEE207 pKa = 5.94FYY209 pKa = 10.87QSWFDD214 pKa = 4.35FYY216 pKa = 10.86PLYY219 pKa = 10.67AAEE222 pKa = 4.56SGGTLLVEE230 pKa = 5.3DD231 pKa = 4.85GASTFAGEE239 pKa = 4.31EE240 pKa = 4.28GTAVADD246 pKa = 4.99FWRR249 pKa = 11.84TIYY252 pKa = 10.48DD253 pKa = 3.33EE254 pKa = 4.51KK255 pKa = 11.22LAGNEE260 pKa = 4.49PYY262 pKa = 10.43TGDD265 pKa = 3.53AFNDD269 pKa = 3.94GVAAIASAGPWAVEE283 pKa = 4.24SYY285 pKa = 11.63GDD287 pKa = 4.05IDD289 pKa = 3.69WGVAPIPTSTGTAPEE304 pKa = 4.02EE305 pKa = 4.53TYY307 pKa = 10.84TFADD311 pKa = 3.72SKK313 pKa = 11.02NVGMYY318 pKa = 10.08SSCEE322 pKa = 3.84NTGTAWEE329 pKa = 4.1FLKK332 pKa = 11.05FSTSEE337 pKa = 4.09EE338 pKa = 3.9QDD340 pKa = 3.14GTLLEE345 pKa = 4.66TTGQMPLRR353 pKa = 11.84ANLTDD358 pKa = 4.15AFPDD362 pKa = 3.78YY363 pKa = 10.64FATNPDD369 pKa = 3.45YY370 pKa = 8.44TTFADD375 pKa = 3.6QANRR379 pKa = 11.84TVDD382 pKa = 3.4VPNVEE387 pKa = 4.13NSVEE391 pKa = 3.97VWQTFRR397 pKa = 11.84DD398 pKa = 3.51AWTSAVIFGEE408 pKa = 4.07DD409 pKa = 4.23EE410 pKa = 3.93IPSALKK416 pKa = 10.56DD417 pKa = 3.34AAGTIDD423 pKa = 3.58EE424 pKa = 4.82LAVKK428 pKa = 10.14KK429 pKa = 10.7

Molecular weight: 45.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q5MYZ6|A0A4Q5MYZ6_9CELL GNAT family N-acetyltransferase OS=Cellulomonas sp. HLT2-17 OX=1259133 GN=EUA98_18625 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3785

0

3785

1270143

30

2576

335.6

35.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.449 ± 0.065

0.594 ± 0.01

6.078 ± 0.036

4.991 ± 0.038

2.763 ± 0.024

9.304 ± 0.037

2.007 ± 0.018

3.752 ± 0.03

1.568 ± 0.025

10.583 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.734 ± 0.017

1.682 ± 0.022

5.747 ± 0.037

2.679 ± 0.018

7.488 ± 0.052

5.33 ± 0.029

6.378 ± 0.04

9.59 ± 0.036

1.475 ± 0.016

1.808 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski