Thermodesulfobium narugense DSM 14796

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermodesulfobiaceae; Thermodesulfobium; Thermodesulfobium narugense

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1797 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1E4F8|M1E4F8_9FIRM Fructose-1 6-bisphosphate aldolase/phosphatase OS=Thermodesulfobium narugense DSM 14796 OX=747365 GN=Thena_0472 PE=3 SV=1
MM1 pKa = 8.02DD2 pKa = 5.53KK3 pKa = 10.63YY4 pKa = 11.43VCTICGYY11 pKa = 10.8VYY13 pKa = 10.62DD14 pKa = 4.79PAVGDD19 pKa = 3.99PDD21 pKa = 5.03GGIAPNTPFEE31 pKa = 4.43QIPDD35 pKa = 3.49DD36 pKa = 4.07WQCPVCGASKK46 pKa = 11.03SDD48 pKa = 4.3FKK50 pKa = 11.46KK51 pKa = 10.52QDD53 pKa = 2.89

Molecular weight: 5.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1E9G3|M1E9G3_9FIRM Glutaredoxin OS=Thermodesulfobium narugense DSM 14796 OX=747365 GN=Thena_1619 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.94RR4 pKa = 11.84TYY6 pKa = 9.0QPKK9 pKa = 8.77VRR11 pKa = 11.84KK12 pKa = 9.25KK13 pKa = 9.7FRR15 pKa = 11.84VHH17 pKa = 6.47GFLSRR22 pKa = 11.84MSTKK26 pKa = 10.48AGRR29 pKa = 11.84KK30 pKa = 7.99VIKK33 pKa = 9.78ARR35 pKa = 11.84RR36 pKa = 11.84MKK38 pKa = 10.3GRR40 pKa = 11.84HH41 pKa = 5.77KK42 pKa = 10.34LTVSAA47 pKa = 5.3

Molecular weight: 5.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1797

0

1797

573739

30

1517

319.3

36.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.759 ± 0.057

1.106 ± 0.023

5.231 ± 0.044

6.808 ± 0.068

5.769 ± 0.061

6.207 ± 0.054

1.403 ± 0.02

9.251 ± 0.058

8.356 ± 0.062

10.209 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.192 ± 0.023

5.43 ± 0.056

3.589 ± 0.035

2.354 ± 0.03

3.942 ± 0.042

7.101 ± 0.053

4.396 ± 0.038

6.422 ± 0.047

0.809 ± 0.022

3.668 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski