Thiohalobacter thiocyanaticus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiales incertae sedis; Thiohalobacter

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3024 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z4VRQ8|A0A1Z4VRQ8_9GAMM Cyclic nucleotide-binding protein OS=Thiohalobacter thiocyanaticus OX=585455 GN=FOKN1_1936 PE=4 SV=1
MM1 pKa = 7.81GIDD4 pKa = 4.11LPAAAAEE11 pKa = 4.28RR12 pKa = 11.84LRR14 pKa = 11.84HH15 pKa = 5.84HH16 pKa = 6.91PLVADD21 pKa = 3.94VEE23 pKa = 4.48QARR26 pKa = 11.84SFEE29 pKa = 4.14LHH31 pKa = 6.11AQQLPTGIDD40 pKa = 3.34RR41 pKa = 11.84INAEE45 pKa = 4.29LHH47 pKa = 6.19PGAGIDD53 pKa = 3.81GSEE56 pKa = 4.23SLVDD60 pKa = 3.41VDD62 pKa = 4.83IAIIDD67 pKa = 3.67TGVEE71 pKa = 4.14LNHH74 pKa = 7.34PDD76 pKa = 3.54LNVYY80 pKa = 9.68QYY82 pKa = 11.26AYY84 pKa = 10.01CYY86 pKa = 10.34QKK88 pKa = 9.4TPRR91 pKa = 11.84SGTCNTGDD99 pKa = 3.27SRR101 pKa = 11.84ANDD104 pKa = 3.56VQGHH108 pKa = 4.8GTHH111 pKa = 5.91VAGIAAARR119 pKa = 11.84DD120 pKa = 3.48NGIGVVGVAPGARR133 pKa = 11.84IWALNVFGTDD143 pKa = 3.24PGAGTGLLEE152 pKa = 4.96IIAALDD158 pKa = 3.63YY159 pKa = 11.24VIANAAQIEE168 pKa = 4.64VVNMSLGFTHH178 pKa = 7.74DD179 pKa = 3.57GTGSRR184 pKa = 11.84NFNEE188 pKa = 5.64AIDD191 pKa = 3.83NTLAAGITVVTSAGNDD207 pKa = 3.99AIDD210 pKa = 4.03VMYY213 pKa = 9.68TSPADD218 pKa = 3.45HH219 pKa = 7.26PGVITVSALADD230 pKa = 3.49GDD232 pKa = 3.75GRR234 pKa = 11.84IGARR238 pKa = 11.84GSLGFTYY245 pKa = 9.21ATADD249 pKa = 3.63GKK251 pKa = 9.61CTEE254 pKa = 4.32KK255 pKa = 10.75QDD257 pKa = 5.16DD258 pKa = 4.03SFACFSNYY266 pKa = 7.91GTGVDD271 pKa = 3.38IMAPGVEE278 pKa = 4.01IHH280 pKa = 5.98STYY283 pKa = 11.03LDD285 pKa = 3.69DD286 pKa = 5.93DD287 pKa = 4.14YY288 pKa = 11.49EE289 pKa = 4.39YY290 pKa = 11.82LHH292 pKa = 6.2GTSMASPHH300 pKa = 5.49VAGAAALYY308 pKa = 9.97LAANPGAGPADD319 pKa = 3.54VKK321 pKa = 11.01AALIAMGDD329 pKa = 3.88PAPCGASLSLCGDD342 pKa = 3.94DD343 pKa = 4.28PDD345 pKa = 6.03GIQEE349 pKa = 4.3PLVLLAPHH357 pKa = 7.78PDD359 pKa = 3.36TDD361 pKa = 4.34ADD363 pKa = 4.13TVLDD367 pKa = 5.11DD368 pKa = 6.09LDD370 pKa = 4.1NCPDD374 pKa = 3.34VHH376 pKa = 8.04NPDD379 pKa = 3.19QADD382 pKa = 3.64RR383 pKa = 11.84DD384 pKa = 3.73GDD386 pKa = 4.34GIGDD390 pKa = 3.75VCDD393 pKa = 3.99SDD395 pKa = 5.6GDD397 pKa = 4.54GIDD400 pKa = 4.09NGSDD404 pKa = 3.36NCPDD408 pKa = 3.51TANADD413 pKa = 3.5QRR415 pKa = 11.84DD416 pKa = 3.54QDD418 pKa = 3.47GDD420 pKa = 4.36GIGDD424 pKa = 3.71ACDD427 pKa = 3.27NCILHH432 pKa = 6.99ANAPQRR438 pKa = 11.84DD439 pKa = 3.87TDD441 pKa = 3.47GDD443 pKa = 4.36GYY445 pKa = 11.27GNRR448 pKa = 11.84CDD450 pKa = 5.29ADD452 pKa = 3.93FNNDD456 pKa = 2.9GAVNFSDD463 pKa = 4.48LAYY466 pKa = 10.36LKK468 pKa = 10.77SAFFSTDD475 pKa = 2.44PHH477 pKa = 7.47ADD479 pKa = 3.47LNGDD483 pKa = 3.65GAVNFADD490 pKa = 4.3LAILKK495 pKa = 10.03SFFFSTPGPSALDD508 pKa = 3.54PP509 pKa = 4.54

Molecular weight: 52.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z4VM51|A0A1Z4VM51_9GAMM Uncharacterized protein OS=Thiohalobacter thiocyanaticus OX=585455 GN=FOKN1_0292 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.88GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.83NGRR28 pKa = 11.84RR29 pKa = 11.84VLKK32 pKa = 10.45ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84ARR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3024

0

3024

944797

43

1891

312.4

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.977 ± 0.06

0.936 ± 0.016

5.919 ± 0.034

6.654 ± 0.048

3.382 ± 0.03

8.133 ± 0.044

2.434 ± 0.021

4.888 ± 0.036

2.715 ± 0.04

11.308 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.325 ± 0.022

2.68 ± 0.025

5.061 ± 0.038

4.031 ± 0.034

7.832 ± 0.048

4.928 ± 0.036

4.796 ± 0.033

6.971 ± 0.041

1.377 ± 0.019

2.652 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski