Red clover associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Luteovirus; unclassified Luteovirus

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G1PZ43|A0A3G1PZ43_9LUTE Readthrough protein OS=Red clover associated virus OX=2162643 PE=3 SV=1
MM1 pKa = 7.54EE2 pKa = 4.13VQILRR7 pKa = 11.84DD8 pKa = 3.88TTHH11 pKa = 6.94TDD13 pKa = 2.87ADD15 pKa = 4.03NNAGPWEE22 pKa = 4.28TGSFSTLSMRR32 pKa = 11.84TGKK35 pKa = 10.49ANRR38 pKa = 11.84QVRR41 pKa = 11.84LVTSLLYY48 pKa = 10.48HH49 pKa = 6.87LLRR52 pKa = 11.84SLSLCSEE59 pKa = 4.49TLPPEE64 pKa = 3.98

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G1PZ46|A0A3G1PZ46_9LUTE RNA-directed RNA polymerase OS=Red clover associated virus OX=2162643 PE=4 SV=1
MM1 pKa = 7.15VARR4 pKa = 11.84NANRR8 pKa = 11.84GRR10 pKa = 11.84RR11 pKa = 11.84AGRR14 pKa = 11.84TSRR17 pKa = 11.84RR18 pKa = 11.84ISQVLMPVRR27 pKa = 11.84TVAMKK32 pKa = 10.73NGNKK36 pKa = 9.51RR37 pKa = 11.84NAGQGLSAGKK47 pKa = 9.65AHH49 pKa = 6.58SEE51 pKa = 4.13VFRR54 pKa = 11.84FTIDD58 pKa = 3.46DD59 pKa = 3.99LKK61 pKa = 11.49GNTSGCYY68 pKa = 9.6KK69 pKa = 10.13FCKK72 pKa = 10.2DD73 pKa = 3.12LTQYY77 pKa = 11.25KK78 pKa = 9.26PISEE82 pKa = 4.69GIIKK86 pKa = 10.28AFHH89 pKa = 7.01EE90 pKa = 4.21YY91 pKa = 10.33RR92 pKa = 11.84VTKK95 pKa = 10.81LNVQYY100 pKa = 10.86RR101 pKa = 11.84SFASAQTTGSLAIEE115 pKa = 4.67IDD117 pKa = 3.61TSCTVTSLGSKK128 pKa = 9.77IEE130 pKa = 4.01SFPIKK135 pKa = 10.44SSASKK140 pKa = 10.48SFSSGALHH148 pKa = 5.71GTNWVNSLQNQFHH161 pKa = 6.69LLISGNGGSEE171 pKa = 3.93VAGQIVVTATIQFQNPKK188 pKa = 10.23

Molecular weight: 20.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1999

64

886

399.8

44.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.104 ± 0.767

2.251 ± 0.147

3.952 ± 0.285

6.903 ± 0.877

4.602 ± 0.353

5.253 ± 1.148

1.701 ± 0.141

5.053 ± 0.244

7.254 ± 0.671

8.204 ± 1.395

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.901 ± 0.299

3.802 ± 0.54

5.403 ± 0.526

3.852 ± 0.318

6.053 ± 0.348

8.354 ± 0.905

6.053 ± 0.749

7.004 ± 0.314

1.301 ± 0.163

2.951 ± 0.391
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski