Amino acid dipepetide frequency for Mycobacterium phage Jsquared

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.07AlaAla: 10.07 ± 0.953
0.789AlaCys: 0.789 ± 0.244
5.581AlaAsp: 5.581 ± 0.656
8.129AlaGlu: 8.129 ± 0.76
3.518AlaPhe: 3.518 ± 0.538
8.007AlaGly: 8.007 ± 1.012
1.82AlaHis: 1.82 ± 0.424
4.368AlaIle: 4.368 ± 0.486
5.217AlaLys: 5.217 ± 0.585
8.614AlaLeu: 8.614 ± 0.886
3.094AlaMet: 3.094 ± 0.397
3.215AlaAsn: 3.215 ± 0.507
4.732AlaPro: 4.732 ± 0.674
4.064AlaGln: 4.064 ± 0.578
5.217AlaArg: 5.217 ± 0.585
4.246AlaSer: 4.246 ± 0.556
4.914AlaThr: 4.914 ± 0.571
6.491AlaVal: 6.491 ± 0.524
1.941AlaTrp: 1.941 ± 0.331
2.548AlaTyr: 2.548 ± 0.506
0.0AlaXaa: 0.0 ± 0.0
Cys
0.607CysAla: 0.607 ± 0.21
0.0CysCys: 0.0 ± 0.0
0.789CysAsp: 0.789 ± 0.191
0.607CysGlu: 0.607 ± 0.22
0.425CysPhe: 0.425 ± 0.16
0.728CysGly: 0.728 ± 0.228
0.182CysHis: 0.182 ± 0.099
0.243CysIle: 0.243 ± 0.115
0.485CysLys: 0.485 ± 0.151
0.728CysLeu: 0.728 ± 0.219
0.303CysMet: 0.303 ± 0.139
0.303CysAsn: 0.303 ± 0.131
0.667CysPro: 0.667 ± 0.23
0.182CysGln: 0.182 ± 0.103
0.728CysArg: 0.728 ± 0.197
0.425CysSer: 0.425 ± 0.182
0.546CysThr: 0.546 ± 0.17
0.546CysVal: 0.546 ± 0.17
0.364CysTrp: 0.364 ± 0.153
0.243CysTyr: 0.243 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
6.309AspAla: 6.309 ± 0.563
0.728AspCys: 0.728 ± 0.27
3.822AspAsp: 3.822 ± 0.492
4.732AspGlu: 4.732 ± 0.631
2.548AspPhe: 2.548 ± 0.417
5.46AspGly: 5.46 ± 0.624
1.638AspHis: 1.638 ± 0.335
3.215AspIle: 3.215 ± 0.439
2.366AspLys: 2.366 ± 0.297
5.278AspLeu: 5.278 ± 0.564
1.577AspMet: 1.577 ± 0.301
1.577AspAsn: 1.577 ± 0.34
4.246AspPro: 4.246 ± 0.577
2.062AspGln: 2.062 ± 0.336
3.276AspArg: 3.276 ± 0.491
2.79AspSer: 2.79 ± 0.429
3.336AspThr: 3.336 ± 0.474
4.55AspVal: 4.55 ± 0.502
1.395AspTrp: 1.395 ± 0.329
2.487AspTyr: 2.487 ± 0.394
0.0AspXaa: 0.0 ± 0.0
Glu
6.733GluAla: 6.733 ± 0.797
0.243GluCys: 0.243 ± 0.109
4.489GluAsp: 4.489 ± 0.582
5.096GluGlu: 5.096 ± 0.756
2.972GluPhe: 2.972 ± 0.413
5.702GluGly: 5.702 ± 0.533
1.456GluHis: 1.456 ± 0.303
4.004GluIle: 4.004 ± 0.493
2.487GluLys: 2.487 ± 0.389
7.522GluLeu: 7.522 ± 0.591
2.487GluMet: 2.487 ± 0.357
2.548GluAsn: 2.548 ± 0.367
2.972GluPro: 2.972 ± 0.543
1.699GluGln: 1.699 ± 0.273
4.853GluArg: 4.853 ± 0.485
3.033GluSer: 3.033 ± 0.505
3.761GluThr: 3.761 ± 0.444
4.974GluVal: 4.974 ± 0.668
1.395GluTrp: 1.395 ± 0.308
2.487GluTyr: 2.487 ± 0.334
0.0GluXaa: 0.0 ± 0.0
Phe
3.7PheAla: 3.7 ± 0.482
0.303PheCys: 0.303 ± 0.138
2.608PheAsp: 2.608 ± 0.441
2.73PheGlu: 2.73 ± 0.365
0.728PhePhe: 0.728 ± 0.208
3.033PheGly: 3.033 ± 0.393
0.667PheHis: 0.667 ± 0.228
1.335PheIle: 1.335 ± 0.283
1.335PheLys: 1.335 ± 0.296
1.941PheLeu: 1.941 ± 0.352
0.425PheMet: 0.425 ± 0.184
1.517PheAsn: 1.517 ± 0.265
1.759PhePro: 1.759 ± 0.362
1.335PheGln: 1.335 ± 0.346
2.79PheArg: 2.79 ± 0.425
1.82PheSer: 1.82 ± 0.353
2.366PheThr: 2.366 ± 0.323
2.487PheVal: 2.487 ± 0.417
0.546PheTrp: 0.546 ± 0.181
1.031PheTyr: 1.031 ± 0.278
0.0PheXaa: 0.0 ± 0.0
Gly
7.34GlyAla: 7.34 ± 0.921
0.728GlyCys: 0.728 ± 0.206
6.127GlyAsp: 6.127 ± 0.65
4.671GlyGlu: 4.671 ± 0.434
3.154GlyPhe: 3.154 ± 0.412
8.675GlyGly: 8.675 ± 1.476
1.517GlyHis: 1.517 ± 0.297
3.882GlyIle: 3.882 ± 0.538
4.914GlyLys: 4.914 ± 0.426
6.248GlyLeu: 6.248 ± 0.69
2.123GlyMet: 2.123 ± 0.464
2.851GlyAsn: 2.851 ± 0.355
2.79GlyPro: 2.79 ± 0.37
3.094GlyGln: 3.094 ± 0.387
4.55GlyArg: 4.55 ± 0.503
3.518GlySer: 3.518 ± 0.491
5.217GlyThr: 5.217 ± 0.63
6.794GlyVal: 6.794 ± 0.708
1.638GlyTrp: 1.638 ± 0.302
2.426GlyTyr: 2.426 ± 0.346
0.0GlyXaa: 0.0 ± 0.0
His
1.759HisAla: 1.759 ± 0.312
0.364HisCys: 0.364 ± 0.128
1.638HisAsp: 1.638 ± 0.346
1.577HisGlu: 1.577 ± 0.359
0.425HisPhe: 0.425 ± 0.147
1.517HisGly: 1.517 ± 0.361
0.789HisHis: 0.789 ± 0.22
1.638HisIle: 1.638 ± 0.351
1.031HisLys: 1.031 ± 0.271
1.517HisLeu: 1.517 ± 0.283
0.182HisMet: 0.182 ± 0.1
0.485HisAsn: 0.485 ± 0.149
1.335HisPro: 1.335 ± 0.295
0.789HisGln: 0.789 ± 0.202
1.638HisArg: 1.638 ± 0.315
0.789HisSer: 0.789 ± 0.219
1.092HisThr: 1.092 ± 0.257
1.092HisVal: 1.092 ± 0.299
0.303HisTrp: 0.303 ± 0.163
0.849HisTyr: 0.849 ± 0.333
0.0HisXaa: 0.0 ± 0.0
Ile
5.278IleAla: 5.278 ± 0.554
0.364IleCys: 0.364 ± 0.151
3.579IleAsp: 3.579 ± 0.416
4.792IleGlu: 4.792 ± 0.537
1.335IlePhe: 1.335 ± 0.322
3.64IleGly: 3.64 ± 0.502
1.153IleHis: 1.153 ± 0.224
1.82IleIle: 1.82 ± 0.278
2.669IleLys: 2.669 ± 0.345
4.004IleLeu: 4.004 ± 0.408
0.364IleMet: 0.364 ± 0.127
1.638IleAsn: 1.638 ± 0.294
3.579IlePro: 3.579 ± 0.429
1.638IleGln: 1.638 ± 0.301
3.154IleArg: 3.154 ± 0.458
2.487IleSer: 2.487 ± 0.477
2.972IleThr: 2.972 ± 0.416
3.094IleVal: 3.094 ± 0.336
0.667IleTrp: 0.667 ± 0.2
0.91IleTyr: 0.91 ± 0.211
0.0IleXaa: 0.0 ± 0.0
Lys
5.278LysAla: 5.278 ± 0.617
0.303LysCys: 0.303 ± 0.149
2.79LysAsp: 2.79 ± 0.409
2.123LysGlu: 2.123 ± 0.336
1.153LysPhe: 1.153 ± 0.237
3.518LysGly: 3.518 ± 0.56
0.667LysHis: 0.667 ± 0.205
2.305LysIle: 2.305 ± 0.382
3.215LysLys: 3.215 ± 0.672
3.822LysLeu: 3.822 ± 0.529
1.213LysMet: 1.213 ± 0.297
1.274LysAsn: 1.274 ± 0.302
4.004LysPro: 4.004 ± 0.586
1.638LysGln: 1.638 ± 0.319
3.822LysArg: 3.822 ± 0.546
2.426LysSer: 2.426 ± 0.368
2.73LysThr: 2.73 ± 0.351
4.61LysVal: 4.61 ± 0.539
0.728LysTrp: 0.728 ± 0.206
1.517LysTyr: 1.517 ± 0.375
0.0LysXaa: 0.0 ± 0.0
Leu
8.432LeuAla: 8.432 ± 0.778
0.667LeuCys: 0.667 ± 0.215
4.974LeuAsp: 4.974 ± 0.514
5.945LeuGlu: 5.945 ± 0.592
2.608LeuPhe: 2.608 ± 0.343
6.551LeuGly: 6.551 ± 0.967
2.002LeuHis: 2.002 ± 0.411
4.732LeuIle: 4.732 ± 0.54
3.7LeuLys: 3.7 ± 0.436
5.763LeuLeu: 5.763 ± 0.519
2.366LeuMet: 2.366 ± 0.321
2.73LeuAsn: 2.73 ± 0.44
4.186LeuPro: 4.186 ± 0.526
2.79LeuGln: 2.79 ± 0.651
5.217LeuArg: 5.217 ± 0.578
5.035LeuSer: 5.035 ± 0.548
4.428LeuThr: 4.428 ± 0.665
4.671LeuVal: 4.671 ± 0.539
1.699LeuTrp: 1.699 ± 0.312
2.305LeuTyr: 2.305 ± 0.374
0.0LeuXaa: 0.0 ± 0.0
Met
2.972MetAla: 2.972 ± 0.482
0.121MetCys: 0.121 ± 0.098
1.092MetAsp: 1.092 ± 0.269
1.638MetGlu: 1.638 ± 0.301
0.303MetPhe: 0.303 ± 0.142
1.577MetGly: 1.577 ± 0.31
0.485MetHis: 0.485 ± 0.179
1.213MetIle: 1.213 ± 0.264
1.941MetLys: 1.941 ± 0.322
1.577MetLeu: 1.577 ± 0.341
0.607MetMet: 0.607 ± 0.199
0.728MetAsn: 0.728 ± 0.198
1.577MetPro: 1.577 ± 0.367
1.274MetGln: 1.274 ± 0.324
1.699MetArg: 1.699 ± 0.291
2.548MetSer: 2.548 ± 0.327
2.184MetThr: 2.184 ± 0.291
1.395MetVal: 1.395 ± 0.284
0.243MetTrp: 0.243 ± 0.1
0.485MetTyr: 0.485 ± 0.156
0.0MetXaa: 0.0 ± 0.0
Asn
2.912AsnAla: 2.912 ± 0.459
0.546AsnCys: 0.546 ± 0.166
1.699AsnAsp: 1.699 ± 0.357
2.244AsnGlu: 2.244 ± 0.437
1.031AsnPhe: 1.031 ± 0.261
3.7AsnGly: 3.7 ± 0.428
0.789AsnHis: 0.789 ± 0.225
1.213AsnIle: 1.213 ± 0.314
0.971AsnLys: 0.971 ± 0.269
2.487AsnLeu: 2.487 ± 0.374
0.971AsnMet: 0.971 ± 0.26
0.91AsnAsn: 0.91 ± 0.237
2.244AsnPro: 2.244 ± 0.381
1.274AsnGln: 1.274 ± 0.282
2.608AsnArg: 2.608 ± 0.386
1.153AsnSer: 1.153 ± 0.248
2.184AsnThr: 2.184 ± 0.448
2.244AsnVal: 2.244 ± 0.331
0.728AsnTrp: 0.728 ± 0.213
1.092AsnTyr: 1.092 ± 0.236
0.0AsnXaa: 0.0 ± 0.0
Pro
4.61ProAla: 4.61 ± 0.575
0.485ProCys: 0.485 ± 0.175
3.518ProAsp: 3.518 ± 0.437
4.004ProGlu: 4.004 ± 0.525
2.062ProPhe: 2.062 ± 0.375
4.55ProGly: 4.55 ± 0.505
1.213ProHis: 1.213 ± 0.261
2.912ProIle: 2.912 ± 0.299
2.487ProLys: 2.487 ± 0.569
3.458ProLeu: 3.458 ± 0.453
1.274ProMet: 1.274 ± 0.313
2.244ProAsn: 2.244 ± 0.377
2.123ProPro: 2.123 ± 0.418
1.759ProGln: 1.759 ± 0.366
3.397ProArg: 3.397 ± 0.554
2.608ProSer: 2.608 ± 0.423
3.518ProThr: 3.518 ± 0.548
4.004ProVal: 4.004 ± 0.456
1.153ProTrp: 1.153 ± 0.347
1.335ProTyr: 1.335 ± 0.277
0.0ProXaa: 0.0 ± 0.0
Gln
3.7GlnAla: 3.7 ± 0.492
0.243GlnCys: 0.243 ± 0.107
1.274GlnAsp: 1.274 ± 0.329
1.88GlnGlu: 1.88 ± 0.353
1.699GlnPhe: 1.699 ± 0.314
3.033GlnGly: 3.033 ± 0.495
0.849GlnHis: 0.849 ± 0.223
2.002GlnIle: 2.002 ± 0.383
1.88GlnLys: 1.88 ± 0.353
3.579GlnLeu: 3.579 ± 0.647
1.153GlnMet: 1.153 ± 0.29
1.213GlnAsn: 1.213 ± 0.303
1.213GlnPro: 1.213 ± 0.305
1.82GlnGln: 1.82 ± 0.348
2.608GlnArg: 2.608 ± 0.419
1.577GlnSer: 1.577 ± 0.387
2.184GlnThr: 2.184 ± 0.449
2.244GlnVal: 2.244 ± 0.334
1.092GlnTrp: 1.092 ± 0.282
1.031GlnTyr: 1.031 ± 0.234
0.0GlnXaa: 0.0 ± 0.0
Arg
5.581ArgAla: 5.581 ± 0.656
1.213ArgCys: 1.213 ± 0.284
4.004ArgAsp: 4.004 ± 0.496
5.399ArgGlu: 5.399 ± 0.745
2.366ArgPhe: 2.366 ± 0.453
3.943ArgGly: 3.943 ± 0.6
1.031ArgHis: 1.031 ± 0.313
4.125ArgIle: 4.125 ± 0.552
3.822ArgLys: 3.822 ± 0.439
5.46ArgLeu: 5.46 ± 0.52
2.062ArgMet: 2.062 ± 0.359
2.123ArgAsn: 2.123 ± 0.342
2.487ArgPro: 2.487 ± 0.435
2.366ArgGln: 2.366 ± 0.403
5.096ArgArg: 5.096 ± 0.596
3.276ArgSer: 3.276 ± 0.492
2.426ArgThr: 2.426 ± 0.311
5.399ArgVal: 5.399 ± 0.467
1.395ArgTrp: 1.395 ± 0.246
2.426ArgTyr: 2.426 ± 0.417
0.0ArgXaa: 0.0 ± 0.0
Ser
4.732SerAla: 4.732 ± 0.413
0.364SerCys: 0.364 ± 0.151
3.397SerAsp: 3.397 ± 0.461
3.518SerGlu: 3.518 ± 0.543
2.305SerPhe: 2.305 ± 0.373
4.61SerGly: 4.61 ± 0.734
0.91SerHis: 0.91 ± 0.241
2.184SerIle: 2.184 ± 0.372
2.244SerLys: 2.244 ± 0.417
4.125SerLeu: 4.125 ± 0.67
1.092SerMet: 1.092 ± 0.236
1.335SerAsn: 1.335 ± 0.341
3.276SerPro: 3.276 ± 0.451
1.88SerGln: 1.88 ± 0.331
3.943SerArg: 3.943 ± 0.584
2.548SerSer: 2.548 ± 0.546
2.73SerThr: 2.73 ± 0.396
3.215SerVal: 3.215 ± 0.398
1.395SerTrp: 1.395 ± 0.334
0.971SerTyr: 0.971 ± 0.232
0.0SerXaa: 0.0 ± 0.0
Thr
6.127ThrAla: 6.127 ± 0.577
0.485ThrCys: 0.485 ± 0.173
3.579ThrAsp: 3.579 ± 0.477
3.154ThrGlu: 3.154 ± 0.423
2.184ThrPhe: 2.184 ± 0.372
4.974ThrGly: 4.974 ± 0.628
1.031ThrHis: 1.031 ± 0.223
2.426ThrIle: 2.426 ± 0.464
3.215ThrLys: 3.215 ± 0.458
4.61ThrLeu: 4.61 ± 0.679
1.395ThrMet: 1.395 ± 0.302
2.002ThrAsn: 2.002 ± 0.264
4.004ThrPro: 4.004 ± 0.461
2.184ThrGln: 2.184 ± 0.324
3.276ThrArg: 3.276 ± 0.43
2.851ThrSer: 2.851 ± 0.48
3.094ThrThr: 3.094 ± 0.502
5.035ThrVal: 5.035 ± 0.554
0.91ThrTrp: 0.91 ± 0.222
1.759ThrTyr: 1.759 ± 0.368
0.0ThrXaa: 0.0 ± 0.0
Val
6.491ValAla: 6.491 ± 0.734
0.789ValCys: 0.789 ± 0.202
5.096ValAsp: 5.096 ± 0.577
5.035ValGlu: 5.035 ± 0.527
2.062ValPhe: 2.062 ± 0.457
5.217ValGly: 5.217 ± 0.629
1.577ValHis: 1.577 ± 0.293
3.094ValIle: 3.094 ± 0.431
3.276ValLys: 3.276 ± 0.377
5.884ValLeu: 5.884 ± 0.644
1.395ValMet: 1.395 ± 0.322
2.608ValAsn: 2.608 ± 0.393
3.154ValPro: 3.154 ± 0.537
2.305ValGln: 2.305 ± 0.334
4.914ValArg: 4.914 ± 0.536
4.55ValSer: 4.55 ± 0.608
5.096ValThr: 5.096 ± 0.616
5.156ValVal: 5.156 ± 0.581
1.092ValTrp: 1.092 ± 0.269
2.426ValTyr: 2.426 ± 0.427
0.0ValXaa: 0.0 ± 0.0
Trp
1.699TrpAla: 1.699 ± 0.373
0.243TrpCys: 0.243 ± 0.154
1.153TrpAsp: 1.153 ± 0.259
1.577TrpGlu: 1.577 ± 0.235
0.728TrpPhe: 0.728 ± 0.231
1.395TrpGly: 1.395 ± 0.307
0.789TrpHis: 0.789 ± 0.251
0.849TrpIle: 0.849 ± 0.225
0.789TrpLys: 0.789 ± 0.246
1.213TrpLeu: 1.213 ± 0.306
0.425TrpMet: 0.425 ± 0.151
0.667TrpAsn: 0.667 ± 0.236
0.971TrpPro: 0.971 ± 0.246
1.031TrpGln: 1.031 ± 0.246
0.91TrpArg: 0.91 ± 0.204
1.213TrpSer: 1.213 ± 0.292
1.638TrpThr: 1.638 ± 0.308
1.092TrpVal: 1.092 ± 0.233
0.546TrpTrp: 0.546 ± 0.193
0.667TrpTyr: 0.667 ± 0.222
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.244TyrAla: 2.244 ± 0.38
0.121TyrCys: 0.121 ± 0.09
2.184TyrAsp: 2.184 ± 0.287
2.062TyrGlu: 2.062 ± 0.349
0.849TyrPhe: 0.849 ± 0.205
2.426TyrGly: 2.426 ± 0.416
0.243TyrHis: 0.243 ± 0.135
1.577TyrIle: 1.577 ± 0.278
1.092TyrLys: 1.092 ± 0.286
2.972TyrLeu: 2.972 ± 0.476
1.092TyrMet: 1.092 ± 0.26
1.031TyrAsn: 1.031 ± 0.259
1.395TyrPro: 1.395 ± 0.279
1.092TyrGln: 1.092 ± 0.263
2.366TyrArg: 2.366 ± 0.475
1.88TyrSer: 1.88 ± 0.32
1.88TyrThr: 1.88 ± 0.32
2.123TyrVal: 2.123 ± 0.429
0.364TyrTrp: 0.364 ± 0.177
0.91TyrTyr: 0.91 ± 0.268
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 101 proteins (16486 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski