Grapevine leafroll-associated virus 6

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Ampelovirus; unclassified Ampelovirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8XSN3|G8XSN3_9CLOS p23 OS=Grapevine leafroll-associated virus 6 OX=203168 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 4.12VTLCLSKK9 pKa = 11.03VKK11 pKa = 10.51EE12 pKa = 5.08DD13 pKa = 3.67IPSTCDD19 pKa = 2.9EE20 pKa = 4.47LFYY23 pKa = 10.63MRR25 pKa = 11.84NAGVFAAVLPAEE37 pKa = 4.35RR38 pKa = 11.84LSFFYY43 pKa = 11.02DD44 pKa = 3.06KK45 pKa = 11.02FSIGNNSDD53 pKa = 3.33RR54 pKa = 11.84NKK56 pKa = 10.17YY57 pKa = 9.9RR58 pKa = 11.84EE59 pKa = 4.27LFSFLDD65 pKa = 3.38NLGNVVATNNGNFIKK80 pKa = 10.54FLDD83 pKa = 3.95VDD85 pKa = 3.97SVMDD89 pKa = 3.78FFKK92 pKa = 10.55RR93 pKa = 11.84KK94 pKa = 9.19YY95 pKa = 8.72PGSSSVSNMNKK106 pKa = 8.66TPASDD111 pKa = 4.16YY112 pKa = 11.29CCLTVPALTTFLSSVCNCLEE132 pKa = 3.9GLTTVLAVGFTRR144 pKa = 11.84KK145 pKa = 10.13GDD147 pKa = 3.75TDD149 pKa = 3.93EE150 pKa = 4.67LWSFKK155 pKa = 10.15DD156 pKa = 3.23TFVVFEE162 pKa = 4.04NSGVISTFYY171 pKa = 10.35GDD173 pKa = 5.86NMDD176 pKa = 3.97TSNSALLEE184 pKa = 4.12VLDD187 pKa = 4.66SFGSTSGKK195 pKa = 10.23SDD197 pKa = 3.41FEE199 pKa = 4.29KK200 pKa = 10.98VVNLLSII207 pKa = 4.41

Molecular weight: 22.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8XSN2|G8XSN2_9CLOS Putative viral coat protein OS=Grapevine leafroll-associated virus 6 OX=203168 PE=4 SV=1
MM1 pKa = 7.53ALSATSTYY9 pKa = 10.92VSVSKK14 pKa = 9.94TDD16 pKa = 3.51EE17 pKa = 3.99NFLNLLRR24 pKa = 11.84TFTGKK29 pKa = 10.24NDD31 pKa = 3.37VVSFADD37 pKa = 3.68EE38 pKa = 3.97VLEE41 pKa = 3.83FLNMNFSRR49 pKa = 11.84ISGSFSLGNYY59 pKa = 9.21SCNTWYY65 pKa = 10.89NGVSGAVGAWPDD77 pKa = 3.37SEE79 pKa = 3.89GWGRR83 pKa = 11.84HH84 pKa = 4.59LLANFIVSKK93 pKa = 10.91NLLAHH98 pKa = 6.55MYY100 pKa = 9.89YY101 pKa = 10.19PLASEE106 pKa = 4.06ITAAVATPKK115 pKa = 10.73SVFTDD120 pKa = 2.99KK121 pKa = 10.87LSRR124 pKa = 11.84VGKK127 pKa = 9.09PVTEE131 pKa = 4.22TSSFPYY137 pKa = 9.88KK138 pKa = 10.45VSRR141 pKa = 11.84SDD143 pKa = 2.92AKK145 pKa = 10.68KK146 pKa = 9.75RR147 pKa = 11.84AEE149 pKa = 4.14EE150 pKa = 3.87FGKK153 pKa = 8.22PTKK156 pKa = 9.56HH157 pKa = 5.81TEE159 pKa = 3.84DD160 pKa = 4.74LIFSIGNYY168 pKa = 9.75LGRR171 pKa = 11.84LPTEE175 pKa = 4.32DD176 pKa = 3.16EE177 pKa = 4.43VMGVKK182 pKa = 10.04SLPIAAVRR190 pKa = 11.84KK191 pKa = 10.0GNVLLDD197 pKa = 3.59VGSDD201 pKa = 3.26VTLNEE206 pKa = 4.25RR207 pKa = 11.84KK208 pKa = 9.57LATLNLRR215 pKa = 11.84GKK217 pKa = 9.93NKK219 pKa = 10.48LGDD222 pKa = 3.63PVEE225 pKa = 4.38GVFFKK230 pKa = 10.71TKK232 pKa = 10.16AKK234 pKa = 10.11EE235 pKa = 3.7IEE237 pKa = 4.03AVAFFMFDD245 pKa = 2.97EE246 pKa = 5.41RR247 pKa = 11.84IITDD251 pKa = 3.66ALKK254 pKa = 11.01LPGVQWAILSCIKK267 pKa = 10.36KK268 pKa = 10.48YY269 pKa = 10.46PFQADD274 pKa = 3.36SFEE277 pKa = 4.34DD278 pKa = 4.05RR279 pKa = 11.84IEE281 pKa = 4.13GLVYY285 pKa = 10.56VQAQPRR291 pKa = 11.84KK292 pKa = 8.12MLEE295 pKa = 3.91GLIPMQGVLDD305 pKa = 5.08LDD307 pKa = 3.96TALSKK312 pKa = 11.22GSLLEE317 pKa = 4.19GEE319 pKa = 4.83VFRR322 pKa = 11.84GDD324 pKa = 3.47AKK326 pKa = 10.8VVAPRR331 pKa = 11.84FQKK334 pKa = 9.32NTVGGLLMKK343 pKa = 10.11IGSRR347 pKa = 11.84FSVKK351 pKa = 9.89EE352 pKa = 3.71YY353 pKa = 11.29SEE355 pKa = 4.24LTRR358 pKa = 11.84KK359 pKa = 9.7LVGALLNKK367 pKa = 10.18NLTLDD372 pKa = 3.42VTYY375 pKa = 9.13TQAVLMLLQRR385 pKa = 11.84YY386 pKa = 7.26IHH388 pKa = 6.13YY389 pKa = 7.75RR390 pKa = 11.84TNALRR395 pKa = 11.84FVNLPVKK402 pKa = 10.43LDD404 pKa = 3.67YY405 pKa = 11.0LIKK408 pKa = 10.65SEE410 pKa = 4.13VKK412 pKa = 10.18KK413 pKa = 11.12VDD415 pKa = 3.8FTGVDD420 pKa = 3.62EE421 pKa = 5.16VFSKK425 pKa = 10.58YY426 pKa = 9.14QNTIPEE432 pKa = 4.37IEE434 pKa = 4.48RR435 pKa = 11.84AWCAPLADD443 pKa = 3.87VAYY446 pKa = 9.29MLLKK450 pKa = 10.01STGGSFAKK458 pKa = 10.03WKK460 pKa = 10.24DD461 pKa = 3.71LQDD464 pKa = 3.8VPAHH468 pKa = 5.82MNFDD472 pKa = 3.62FVGYY476 pKa = 10.25VDD478 pKa = 4.55PKK480 pKa = 10.43LLSVNEE486 pKa = 4.7LKK488 pKa = 10.82FQTQLLARR496 pKa = 11.84FRR498 pKa = 11.84SKK500 pKa = 9.46NTPVRR505 pKa = 11.84GFLLGARR512 pKa = 11.84QGNPVDD518 pKa = 3.95YY519 pKa = 10.45LIDD522 pKa = 3.55SAGIGGVEE530 pKa = 3.51RR531 pKa = 11.84SMVKK535 pKa = 10.34RR536 pKa = 11.84LLGG539 pKa = 3.72

Molecular weight: 59.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4451

46

2857

741.8

82.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.403 ± 0.407

1.887 ± 0.313

5.28 ± 0.304

5.841 ± 0.314

5.257 ± 0.549

6.336 ± 0.493

1.483 ± 0.425

4.673 ± 0.347

7.549 ± 0.637

9.211 ± 0.62

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.269 ± 0.144

5.28 ± 0.415

4.493 ± 0.321

2.067 ± 0.306

4.493 ± 0.241

9.728 ± 0.593

5.639 ± 0.33

8.56 ± 0.688

0.741 ± 0.126

2.808 ± 0.401
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski