Amino acid dipepetide frequency for Ectothiorhodospiraceae bacterium BW-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.864AlaAla: 9.864 ± 0.138	0.771AlaCys: 0.771 ± 0.029	4.818AlaAsp: 4.818 ± 0.082	6.634AlaGlu: 6.634 ± 0.095	2.581AlaPhe: 2.581 ± 0.047	5.798AlaGly: 5.798 ± 0.106	1.744AlaHis: 1.744 ± 0.041	6.068AlaIle: 6.068 ± 0.094	3.704AlaLys: 3.704 ± 0.076	11.14AlaLeu: 11.14 ± 0.125	2.517AlaMet: 2.517 ± 0.055	3.214AlaAsn: 3.214 ± 0.064	3.348AlaPro: 3.348 ± 0.071	4.223AlaGln: 4.223 ± 0.073	4.356AlaArg: 4.356 ± 0.072	4.707AlaSer: 4.707 ± 0.085	5.883AlaThr: 5.883 ± 0.16	6.152AlaVal: 6.152 ± 0.088	1.054AlaTrp: 1.054 ± 0.03	2.035AlaTyr: 2.035 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.655CysAla: 0.655 ± 0.024	0.157CysCys: 0.157 ± 0.012	0.598CysAsp: 0.598 ± 0.024	0.571CysGlu: 0.571 ± 0.023	0.381CysPhe: 0.381 ± 0.02	0.994CysGly: 0.994 ± 0.036	0.546CysHis: 0.546 ± 0.031	0.354CysIle: 0.354 ± 0.016	0.236CysLys: 0.236 ± 0.015	0.742CysLeu: 0.742 ± 0.027	0.19CysMet: 0.19 ± 0.014	0.28CysAsn: 0.28 ± 0.018	0.446CysPro: 0.446 ± 0.022	0.57CysGln: 0.57 ± 0.024	0.89CysArg: 0.89 ± 0.03	0.65CysSer: 0.65 ± 0.024	0.341CysThr: 0.341 ± 0.018	0.448CysVal: 0.448 ± 0.022	0.131CysTrp: 0.131 ± 0.015	0.403CysTyr: 0.403 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	3.845AspAla: 3.845 ± 0.073	0.446AspCys: 0.446 ± 0.02	2.715AspAsp: 2.715 ± 0.056	3.999AspGlu: 3.999 ± 0.071	2.013AspPhe: 2.013 ± 0.041	3.702AspGly: 3.702 ± 0.121	1.245AspHis: 1.245 ± 0.033	3.708AspIle: 3.708 ± 0.06	2.1AspLys: 2.1 ± 0.059	4.863AspLeu: 4.863 ± 0.071	1.198AspMet: 1.198 ± 0.039	2.249AspAsn: 2.249 ± 0.051	2.241AspPro: 2.241 ± 0.043	1.951AspGln: 1.951 ± 0.042	3.086AspArg: 3.086 ± 0.055	3.951AspSer: 3.951 ± 0.082	2.633AspThr: 2.633 ± 0.087	2.33AspVal: 2.33 ± 0.052	0.747AspTrp: 0.747 ± 0.028	2.25AspTyr: 2.25 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	6.609GluAla: 6.609 ± 0.095	0.507GluCys: 0.507 ± 0.022	2.1GluAsp: 2.1 ± 0.058	4.203GluGlu: 4.203 ± 0.078	1.881GluPhe: 1.881 ± 0.049	4.387GluGly: 4.387 ± 0.062	1.369GluHis: 1.369 ± 0.04	3.815GluIle: 3.815 ± 0.06	2.213GluLys: 2.213 ± 0.058	8.787GluLeu: 8.787 ± 0.123	1.553GluMet: 1.553 ± 0.035	1.707GluAsn: 1.707 ± 0.052	2.885GluPro: 2.885 ± 0.07	5.605GluGln: 5.605 ± 0.096	5.344GluArg: 5.344 ± 0.093	4.537GluSer: 4.537 ± 0.075	2.956GluThr: 2.956 ± 0.05	4.205GluVal: 4.205 ± 0.077	0.878GluTrp: 0.878 ± 0.027	1.424GluTyr: 1.424 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	2.94PheAla: 2.94 ± 0.055	0.466PheCys: 0.466 ± 0.022	2.454PheAsp: 2.454 ± 0.048	2.512PheGlu: 2.512 ± 0.055	1.306PhePhe: 1.306 ± 0.043	2.644PheGly: 2.644 ± 0.047	0.819PheHis: 0.819 ± 0.027	2.269PheIle: 2.269 ± 0.048	1.304PheLys: 1.304 ± 0.037	2.821PheLeu: 2.821 ± 0.061	0.83PheMet: 0.83 ± 0.027	1.699PheAsn: 1.699 ± 0.044	1.193PhePro: 1.193 ± 0.038	1.246PheGln: 1.246 ± 0.037	1.794PheArg: 1.794 ± 0.043	2.751PheSer: 2.751 ± 0.066	2.1PheThr: 2.1 ± 0.047	1.939PheVal: 1.939 ± 0.042	0.486PheTrp: 0.486 ± 0.022	1.166PheTyr: 1.166 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	5.49GlyAla: 5.49 ± 0.119	0.89GlyCys: 0.89 ± 0.03	3.713GlyAsp: 3.713 ± 0.067	5.647GlyGlu: 5.647 ± 0.103	2.532GlyPhe: 2.532 ± 0.053	5.243GlyGly: 5.243 ± 0.089	1.679GlyHis: 1.679 ± 0.038	3.789GlyIle: 3.789 ± 0.06	2.824GlyLys: 2.824 ± 0.064	6.642GlyLeu: 6.642 ± 0.083	1.726GlyMet: 1.726 ± 0.047	2.157GlyAsn: 2.157 ± 0.07	1.465GlyPro: 1.465 ± 0.037	2.921GlyGln: 2.921 ± 0.061	4.188GlyArg: 4.188 ± 0.071	4.551GlySer: 4.551 ± 0.117	3.142GlyThr: 3.142 ± 0.13	5.186GlyVal: 5.186 ± 0.073	1.041GlyTrp: 1.041 ± 0.034	2.692GlyTyr: 2.692 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	1.766HisAla: 1.766 ± 0.034	0.388HisCys: 0.388 ± 0.017	1.254HisAsp: 1.254 ± 0.034	1.188HisGlu: 1.188 ± 0.035	1.099HisPhe: 1.099 ± 0.031	1.675HisGly: 1.675 ± 0.044	1.185HisHis: 1.185 ± 0.033	1.443HisIle: 1.443 ± 0.041	0.782HisLys: 0.782 ± 0.028	2.799HisLeu: 2.799 ± 0.058	0.315HisMet: 0.315 ± 0.018	0.865HisAsn: 0.865 ± 0.027	1.5HisPro: 1.5 ± 0.041	1.57HisGln: 1.57 ± 0.041	1.666HisArg: 1.666 ± 0.042	1.604HisSer: 1.604 ± 0.041	1.251HisThr: 1.251 ± 0.036	0.739HisVal: 0.739 ± 0.025	0.493HisTrp: 0.493 ± 0.021	1.366HisTyr: 1.366 ± 0.038	0.0HisXaa: 0.0 ± 0.0
Ile	6.179IleAla: 6.179 ± 0.087	0.582IleCys: 0.582 ± 0.023	4.576IleAsp: 4.576 ± 0.059	5.184IleGlu: 5.184 ± 0.079	1.893IlePhe: 1.893 ± 0.042	4.287IleGly: 4.287 ± 0.067	1.454IleHis: 1.454 ± 0.039	3.141IleIle: 3.141 ± 0.06	2.182IleLys: 2.182 ± 0.053	4.702IleLeu: 4.702 ± 0.078	1.057IleMet: 1.057 ± 0.035	2.432IleAsn: 2.432 ± 0.049	2.391IlePro: 2.391 ± 0.045	1.95IleGln: 1.95 ± 0.047	3.238IleArg: 3.238 ± 0.053	3.721IleSer: 3.721 ± 0.066	3.542IleThr: 3.542 ± 0.09	3.598IleVal: 3.598 ± 0.057	0.619IleTrp: 0.619 ± 0.022	1.75IleTyr: 1.75 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	3.05LysAla: 3.05 ± 0.069	0.22LysCys: 0.22 ± 0.017	1.317LysAsp: 1.317 ± 0.038	2.356LysGlu: 2.356 ± 0.061	0.945LysPhe: 0.945 ± 0.033	2.334LysGly: 2.334 ± 0.055	0.611LysHis: 0.611 ± 0.026	2.285LysIle: 2.285 ± 0.053	1.604LysLys: 1.604 ± 0.049	4.167LysLeu: 4.167 ± 0.073	0.917LysMet: 0.917 ± 0.033	1.199LysAsn: 1.199 ± 0.04	1.802LysPro: 1.802 ± 0.048	2.332LysGln: 2.332 ± 0.051	2.732LysArg: 2.732 ± 0.071	2.343LysSer: 2.343 ± 0.052	1.826LysThr: 1.826 ± 0.047	2.221LysVal: 2.221 ± 0.045	0.412LysTrp: 0.412 ± 0.019	0.815LysTyr: 0.815 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	10.85LeuAla: 10.85 ± 0.123	1.074LeuCys: 1.074 ± 0.03	5.552LeuAsp: 5.552 ± 0.085	7.051LeuGlu: 7.051 ± 0.088	4.676LeuPhe: 4.676 ± 0.079	6.766LeuGly: 6.766 ± 0.081	2.657LeuHis: 2.657 ± 0.049	6.364LeuIle: 6.364 ± 0.082	4.71LeuLys: 4.71 ± 0.082	14.635LeuLeu: 14.635 ± 0.214	2.526LeuMet: 2.526 ± 0.053	4.365LeuAsn: 4.365 ± 0.072	6.025LeuPro: 6.025 ± 0.086	7.453LeuGln: 7.453 ± 0.143	6.354LeuArg: 6.354 ± 0.099	7.675LeuSer: 7.675 ± 0.091	7.19LeuThr: 7.19 ± 0.123	6.034LeuVal: 6.034 ± 0.081	1.545LeuTrp: 1.545 ± 0.048	3.422LeuTyr: 3.422 ± 0.063	0.0LeuXaa: 0.0 ± 0.0
Met	2.797MetAla: 2.797 ± 0.058	0.129MetCys: 0.129 ± 0.011	1.0MetAsp: 1.0 ± 0.032	1.463MetGlu: 1.463 ± 0.039	0.532MetPhe: 0.532 ± 0.021	1.656MetGly: 1.656 ± 0.038	0.338MetHis: 0.338 ± 0.018	1.232MetIle: 1.232 ± 0.037	0.816MetLys: 0.816 ± 0.028	2.307MetLeu: 2.307 ± 0.053	0.603MetMet: 0.603 ± 0.021	0.804MetAsn: 0.804 ± 0.025	1.079MetPro: 1.079 ± 0.032	1.136MetGln: 1.136 ± 0.034	1.501MetArg: 1.501 ± 0.033	1.722MetSer: 1.722 ± 0.044	1.294MetThr: 1.294 ± 0.036	1.939MetVal: 1.939 ± 0.049	0.163MetTrp: 0.163 ± 0.012	0.29MetTyr: 0.29 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.522AsnAla: 2.522 ± 0.053	0.353AsnCys: 0.353 ± 0.018	1.848AsnAsp: 1.848 ± 0.055	1.962AsnGlu: 1.962 ± 0.043	1.222AsnPhe: 1.222 ± 0.038	2.661AsnGly: 2.661 ± 0.07	1.069AsnHis: 1.069 ± 0.032	1.951AsnIle: 1.951 ± 0.047	0.886AsnLys: 0.886 ± 0.038	3.433AsnLeu: 3.433 ± 0.061	0.706AsnMet: 0.706 ± 0.024	1.346AsnAsn: 1.346 ± 0.053	1.979AsnPro: 1.979 ± 0.046	1.873AsnGln: 1.873 ± 0.043	3.083AsnArg: 3.083 ± 0.061	2.58AsnSer: 2.58 ± 0.072	1.486AsnThr: 1.486 ± 0.051	1.367AsnVal: 1.367 ± 0.049	0.471AsnTrp: 0.471 ± 0.021	1.279AsnTyr: 1.279 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	3.605ProAla: 3.605 ± 0.07	0.316ProCys: 0.316 ± 0.016	2.682ProAsp: 2.682 ± 0.055	3.393ProGlu: 3.393 ± 0.062	1.832ProPhe: 1.832 ± 0.04	2.218ProGly: 2.218 ± 0.045	1.176ProHis: 1.176 ± 0.036	2.422ProIle: 2.422 ± 0.054	1.686ProLys: 1.686 ± 0.04	6.126ProLeu: 6.126 ± 0.093	1.041ProMet: 1.041 ± 0.031	1.463ProAsn: 1.463 ± 0.04	2.685ProPro: 2.685 ± 0.074	2.53ProGln: 2.53 ± 0.06	1.919ProArg: 1.919 ± 0.045	2.304ProSer: 2.304 ± 0.049	2.522ProThr: 2.522 ± 0.065	2.837ProVal: 2.837 ± 0.052	0.637ProTrp: 0.637 ± 0.027	1.323ProTyr: 1.323 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	4.794GlnAla: 4.794 ± 0.086	0.433GlnCys: 0.433 ± 0.02	1.32GlnAsp: 1.32 ± 0.033	2.407GlnGlu: 2.407 ± 0.053	1.963GlnPhe: 1.963 ± 0.044	3.202GlnGly: 3.202 ± 0.059	1.475GlnHis: 1.475 ± 0.04	3.182GlnIle: 3.182 ± 0.05	1.724GlnLys: 1.724 ± 0.045	9.063GlnLeu: 9.063 ± 0.17	1.349GlnMet: 1.349 ± 0.034	1.443GlnAsn: 1.443 ± 0.045	2.883GlnPro: 2.883 ± 0.058	7.482GlnGln: 7.482 ± 0.206	5.0GlnArg: 5.0 ± 0.101	3.295GlnSer: 3.295 ± 0.061	2.44GlnThr: 2.44 ± 0.058	2.768GlnVal: 2.768 ± 0.05	0.971GlnTrp: 0.971 ± 0.045	0.906GlnTyr: 0.906 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	4.672ArgAla: 4.672 ± 0.071	0.704ArgCys: 0.704 ± 0.032	3.026ArgAsp: 3.026 ± 0.06	4.13ArgGlu: 4.13 ± 0.067	2.87ArgPhe: 2.87 ± 0.063	3.712ArgGly: 3.712 ± 0.063	2.065ArgHis: 2.065 ± 0.052	3.316ArgIle: 3.316 ± 0.06	1.865ArgLys: 1.865 ± 0.044	8.36ArgLeu: 8.36 ± 0.118	1.337ArgMet: 1.337 ± 0.036	1.72ArgAsn: 1.72 ± 0.045	2.518ArgPro: 2.518 ± 0.049	4.733ArgGln: 4.733 ± 0.094	3.999ArgArg: 3.999 ± 0.08	3.398ArgSer: 3.398 ± 0.059	2.484ArgThr: 2.484 ± 0.048	4.002ArgVal: 4.002 ± 0.066	1.059ArgTrp: 1.059 ± 0.031	2.768ArgTyr: 2.768 ± 0.059	0.0ArgXaa: 0.0 ± 0.0
Ser	5.775SerAla: 5.775 ± 0.111	0.714SerCys: 0.714 ± 0.033	3.922SerAsp: 3.922 ± 0.081	4.235SerGlu: 4.235 ± 0.068	2.17SerPhe: 2.17 ± 0.051	5.796SerGly: 5.796 ± 0.119	1.842SerHis: 1.842 ± 0.043	3.126SerIle: 3.126 ± 0.053	1.695SerLys: 1.695 ± 0.042	6.812SerLeu: 6.812 ± 0.084	1.406SerMet: 1.406 ± 0.038	1.836SerAsn: 1.836 ± 0.051	2.746SerPro: 2.746 ± 0.051	2.993SerGln: 2.993 ± 0.056	4.088SerArg: 4.088 ± 0.062	4.105SerSer: 4.105 ± 0.107	2.694SerThr: 2.694 ± 0.087	4.094SerVal: 4.094 ± 0.088	0.962SerTrp: 0.962 ± 0.034	2.195SerTyr: 2.195 ± 0.084	0.0SerXaa: 0.0 ± 0.0
Thr	5.441ThrAla: 5.441 ± 0.186	0.37ThrCys: 0.37 ± 0.017	2.774ThrAsp: 2.774 ± 0.09	3.535ThrGlu: 3.535 ± 0.07	1.536ThrPhe: 1.536 ± 0.047	3.513ThrGly: 3.513 ± 0.099	1.243ThrHis: 1.243 ± 0.035	3.71ThrIle: 3.71 ± 0.069	1.479ThrLys: 1.479 ± 0.043	7.992ThrLeu: 7.992 ± 0.136	1.051ThrMet: 1.051 ± 0.031	1.483ThrAsn: 1.483 ± 0.059	3.257ThrPro: 3.257 ± 0.068	2.165ThrGln: 2.165 ± 0.055	2.462ThrArg: 2.462 ± 0.05	2.566ThrSer: 2.566 ± 0.095	3.568ThrThr: 3.568 ± 0.111	3.617ThrVal: 3.617 ± 0.12	0.436ThrTrp: 0.436 ± 0.023	1.149ThrTyr: 1.149 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	6.881ValAla: 6.881 ± 0.088	0.478ValCys: 0.478 ± 0.021	3.489ValAsp: 3.489 ± 0.062	4.43ValGlu: 4.43 ± 0.07	1.526ValPhe: 1.526 ± 0.036	3.954ValGly: 3.954 ± 0.073	0.986ValHis: 0.986 ± 0.029	4.122ValIle: 4.122 ± 0.072	2.615ValLys: 2.615 ± 0.056	5.537ValLeu: 5.537 ± 0.079	1.699ValMet: 1.699 ± 0.04	2.464ValAsn: 2.464 ± 0.056	2.134ValPro: 2.134 ± 0.04	1.858ValGln: 1.858 ± 0.04	3.33ValArg: 3.33 ± 0.056	3.923ValSer: 3.923 ± 0.079	4.205ValThr: 4.205 ± 0.14	5.46ValVal: 5.46 ± 0.089	0.721ValTrp: 0.721 ± 0.028	1.253ValTyr: 1.253 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	0.699TrpAla: 0.699 ± 0.023	0.189TrpCys: 0.189 ± 0.012	0.544TrpAsp: 0.544 ± 0.02	0.644TrpGlu: 0.644 ± 0.026	0.546TrpPhe: 0.546 ± 0.025	0.824TrpGly: 0.824 ± 0.034	0.438TrpHis: 0.438 ± 0.021	0.696TrpIle: 0.696 ± 0.021	0.32TrpLys: 0.32 ± 0.018	2.179TrpLeu: 2.179 ± 0.052	0.298TrpMet: 0.298 ± 0.015	0.38TrpAsn: 0.38 ± 0.019	0.584TrpPro: 0.584 ± 0.024	1.416TrpGln: 1.416 ± 0.045	1.061TrpArg: 1.061 ± 0.035	0.868TrpSer: 0.868 ± 0.028	0.311TrpThr: 0.311 ± 0.016	0.873TrpVal: 0.873 ± 0.03	0.233TrpTrp: 0.233 ± 0.014	0.39TrpTyr: 0.39 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.175TyrAla: 2.175 ± 0.048	0.398TyrCys: 0.398 ± 0.021	1.569TyrAsp: 1.569 ± 0.04	1.423TyrGlu: 1.423 ± 0.04	1.235TyrPhe: 1.235 ± 0.036	2.152TyrGly: 2.152 ± 0.044	1.01TyrHis: 1.01 ± 0.031	1.482TyrIle: 1.482 ± 0.038	0.741TyrLys: 0.741 ± 0.028	3.819TyrLeu: 3.819 ± 0.062	0.48TyrMet: 0.48 ± 0.023	0.972TyrAsn: 0.972 ± 0.03	1.554TyrPro: 1.554 ± 0.035	2.093TyrGln: 2.093 ± 0.05	2.648TyrArg: 2.648 ± 0.052	1.954TyrSer: 1.954 ± 0.05	1.48TyrThr: 1.48 ± 0.066	1.257TyrVal: 1.257 ± 0.038	0.436TyrTrp: 0.436 ± 0.022	1.049TyrTyr: 1.049 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3445 proteins (1182593 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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