Comamonas sp. JNW

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Comamonas; unclassified Comamonas

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4372 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A363RKU5|A0A363RKU5_9BURK Amino acid ABC transporter permease OS=Comamonas sp. JNW OX=2170731 GN=DCO45_14135 PE=3 SV=1
MM1 pKa = 7.38GGKK4 pKa = 9.33HH5 pKa = 6.23PSAQEE10 pKa = 3.73LLALAEE16 pKa = 3.99QLGIAVADD24 pKa = 3.19SWLYY28 pKa = 10.28IDD30 pKa = 3.74YY31 pKa = 8.24VTWGGIDD38 pKa = 3.14AVYY41 pKa = 10.9AFGSFQGQPFGPIDD55 pKa = 4.29DD56 pKa = 4.85SNLEE60 pKa = 4.41TVEE63 pKa = 3.89MTYY66 pKa = 10.67VEE68 pKa = 4.37VMSCIGISKK77 pKa = 10.54ADD79 pKa = 3.75ALDD82 pKa = 3.72FQPFNRR88 pKa = 11.84GFWGDD93 pKa = 3.1

Molecular weight: 10.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A363RRY6|A0A363RRY6_9BURK ABC transporter substrate-binding protein OS=Comamonas sp. JNW OX=2170731 GN=DCO45_03585 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 8.87VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 9.4TKK25 pKa = 10.4GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.44GRR39 pKa = 11.84KK40 pKa = 9.07RR41 pKa = 11.84LGLL44 pKa = 3.7

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4372

0

4372

1443950

31

3712

330.3

35.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.098 ± 0.059

0.938 ± 0.012

5.029 ± 0.027

4.738 ± 0.035

3.305 ± 0.023

8.251 ± 0.041

2.198 ± 0.02

4.33 ± 0.026

3.313 ± 0.033

10.768 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.598 ± 0.019

2.733 ± 0.03

5.232 ± 0.03

5.134 ± 0.03

6.018 ± 0.035

5.861 ± 0.034

5.067 ± 0.045

7.484 ± 0.035

1.575 ± 0.02

2.33 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski