Amino acid dipepetide frequency for Comamonas sp. JNW

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.569AlaAla: 18.569 ± 0.18
1.329AlaCys: 1.329 ± 0.034
6.493AlaAsp: 6.493 ± 0.075
6.141AlaGlu: 6.141 ± 0.081
3.805AlaPhe: 3.805 ± 0.062
10.599AlaGly: 10.599 ± 0.113
2.763AlaHis: 2.763 ± 0.049
5.424AlaIle: 5.424 ± 0.065
4.206AlaLys: 4.206 ± 0.073
15.351AlaLeu: 15.351 ± 0.147
3.779AlaMet: 3.779 ± 0.049
3.085AlaAsn: 3.085 ± 0.053
6.571AlaPro: 6.571 ± 0.094
8.248AlaGln: 8.248 ± 0.113
7.444AlaArg: 7.444 ± 0.095
7.66AlaSer: 7.66 ± 0.089
6.009AlaThr: 6.009 ± 0.078
8.888AlaVal: 8.888 ± 0.09
1.979AlaTrp: 1.979 ± 0.041
2.631AlaTyr: 2.631 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
1.192CysAla: 1.192 ± 0.027
0.118CysCys: 0.118 ± 0.009
0.492CysAsp: 0.492 ± 0.021
0.449CysGlu: 0.449 ± 0.018
0.305CysPhe: 0.305 ± 0.015
0.916CysGly: 0.916 ± 0.024
0.258CysHis: 0.258 ± 0.013
0.44CysIle: 0.44 ± 0.018
0.287CysLys: 0.287 ± 0.014
0.909CysLeu: 0.909 ± 0.025
0.253CysMet: 0.253 ± 0.014
0.253CysAsn: 0.253 ± 0.012
0.485CysPro: 0.485 ± 0.021
0.363CysGln: 0.363 ± 0.017
0.483CysArg: 0.483 ± 0.018
0.618CysSer: 0.618 ± 0.03
0.553CysThr: 0.553 ± 0.031
0.668CysVal: 0.668 ± 0.021
0.131CysTrp: 0.131 ± 0.011
0.209CysTyr: 0.209 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.896AspAla: 6.896 ± 0.079
0.466AspCys: 0.466 ± 0.018
2.366AspAsp: 2.366 ± 0.043
2.675AspGlu: 2.675 ± 0.05
1.922AspPhe: 1.922 ± 0.038
4.463AspGly: 4.463 ± 0.062
1.009AspHis: 1.009 ± 0.029
2.432AspIle: 2.432 ± 0.048
1.992AspLys: 1.992 ± 0.048
5.04AspLeu: 5.04 ± 0.065
1.379AspMet: 1.379 ± 0.03
1.325AspAsn: 1.325 ± 0.033
2.568AspPro: 2.568 ± 0.042
1.885AspGln: 1.885 ± 0.036
2.659AspArg: 2.659 ± 0.051
2.583AspSer: 2.583 ± 0.045
2.425AspThr: 2.425 ± 0.042
3.79AspVal: 3.79 ± 0.054
0.98AspTrp: 0.98 ± 0.029
1.432AspTyr: 1.432 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
6.228GluAla: 6.228 ± 0.079
0.362GluCys: 0.362 ± 0.016
2.096GluAsp: 2.096 ± 0.04
2.192GluGlu: 2.192 ± 0.053
1.482GluPhe: 1.482 ± 0.036
3.488GluGly: 3.488 ± 0.056
1.147GluHis: 1.147 ± 0.031
2.269GluIle: 2.269 ± 0.045
1.789GluLys: 1.789 ± 0.045
5.146GluLeu: 5.146 ± 0.07
1.229GluMet: 1.229 ± 0.03
1.202GluAsn: 1.202 ± 0.03
2.28GluPro: 2.28 ± 0.046
2.688GluGln: 2.688 ± 0.049
3.962GluArg: 3.962 ± 0.071
2.316GluSer: 2.316 ± 0.042
2.209GluThr: 2.209 ± 0.038
3.721GluVal: 3.721 ± 0.064
0.643GluTrp: 0.643 ± 0.023
0.935GluTyr: 0.935 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
4.191PheAla: 4.191 ± 0.055
0.392PheCys: 0.392 ± 0.018
2.204PheAsp: 2.204 ± 0.046
1.731PheGlu: 1.731 ± 0.037
1.301PhePhe: 1.301 ± 0.043
3.128PheGly: 3.128 ± 0.054
0.672PheHis: 0.672 ± 0.024
1.442PheIle: 1.442 ± 0.037
1.171PheLys: 1.171 ± 0.03
2.721PheLeu: 2.721 ± 0.053
0.823PheMet: 0.823 ± 0.021
1.158PheAsn: 1.158 ± 0.029
1.392PhePro: 1.392 ± 0.028
1.148PheGln: 1.148 ± 0.029
1.503PheArg: 1.503 ± 0.036
2.351PheSer: 2.351 ± 0.043
1.63PheThr: 1.63 ± 0.033
2.69PheVal: 2.69 ± 0.053
0.506PheTrp: 0.506 ± 0.02
0.895PheTyr: 0.895 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
9.173GlyAla: 9.173 ± 0.091
0.878GlyCys: 0.878 ± 0.023
3.806GlyAsp: 3.806 ± 0.061
3.846GlyGlu: 3.846 ± 0.061
3.089GlyPhe: 3.089 ± 0.049
6.946GlyGly: 6.946 ± 0.125
1.785GlyHis: 1.785 ± 0.038
4.073GlyIle: 4.073 ± 0.054
3.402GlyLys: 3.402 ± 0.057
9.137GlyLeu: 9.137 ± 0.116
2.532GlyMet: 2.532 ± 0.044
2.425GlyAsn: 2.425 ± 0.061
2.997GlyPro: 2.997 ± 0.054
4.232GlyGln: 4.232 ± 0.066
4.604GlyArg: 4.604 ± 0.058
5.292GlySer: 5.292 ± 0.089
4.292GlyThr: 4.292 ± 0.082
6.169GlyVal: 6.169 ± 0.068
1.503GlyTrp: 1.503 ± 0.035
2.33GlyTyr: 2.33 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
2.782HisAla: 2.782 ± 0.049
0.283HisCys: 0.283 ± 0.012
1.082HisAsp: 1.082 ± 0.03
0.939HisGlu: 0.939 ± 0.029
0.845HisPhe: 0.845 ± 0.025
1.952HisGly: 1.952 ± 0.042
0.585HisHis: 0.585 ± 0.022
1.009HisIle: 1.009 ± 0.027
0.593HisLys: 0.593 ± 0.02
2.148HisLeu: 2.148 ± 0.041
0.558HisMet: 0.558 ± 0.02
0.537HisAsn: 0.537 ± 0.022
1.445HisPro: 1.445 ± 0.034
1.01HisGln: 1.01 ± 0.025
1.229HisArg: 1.229 ± 0.032
1.269HisSer: 1.269 ± 0.036
1.065HisThr: 1.065 ± 0.028
1.459HisVal: 1.459 ± 0.031
0.519HisTrp: 0.519 ± 0.019
0.674HisTyr: 0.674 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
6.351IleAla: 6.351 ± 0.08
0.454IleCys: 0.454 ± 0.018
2.862IleAsp: 2.862 ± 0.049
2.657IleGlu: 2.657 ± 0.052
1.341IlePhe: 1.341 ± 0.036
3.979IleGly: 3.979 ± 0.055
0.881IleHis: 0.881 ± 0.024
1.637IleIle: 1.637 ± 0.038
1.573IleLys: 1.573 ± 0.041
3.242IleLeu: 3.242 ± 0.048
0.788IleMet: 0.788 ± 0.026
1.526IleAsn: 1.526 ± 0.034
2.042IlePro: 2.042 ± 0.042
1.519IleGln: 1.519 ± 0.032
2.272IleArg: 2.272 ± 0.042
2.738IleSer: 2.738 ± 0.051
2.629IleThr: 2.629 ± 0.051
3.258IleVal: 3.258 ± 0.054
0.528IleTrp: 0.528 ± 0.021
1.024IleTyr: 1.024 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
4.253LysAla: 4.253 ± 0.066
0.163LysCys: 0.163 ± 0.011
1.777LysAsp: 1.777 ± 0.041
1.55LysGlu: 1.55 ± 0.037
0.854LysPhe: 0.854 ± 0.027
2.556LysGly: 2.556 ± 0.053
0.638LysHis: 0.638 ± 0.02
1.527LysIle: 1.527 ± 0.036
1.477LysLys: 1.477 ± 0.045
3.513LysLeu: 3.513 ± 0.063
0.859LysMet: 0.859 ± 0.023
1.017LysAsn: 1.017 ± 0.03
2.079LysPro: 2.079 ± 0.047
1.414LysGln: 1.414 ± 0.037
2.157LysArg: 2.157 ± 0.036
1.754LysSer: 1.754 ± 0.042
2.001LysThr: 2.001 ± 0.043
2.526LysVal: 2.526 ± 0.052
0.345LysTrp: 0.345 ± 0.017
0.664LysTyr: 0.664 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
14.822LeuAla: 14.822 ± 0.142
1.071LeuCys: 1.071 ± 0.028
5.34LeuAsp: 5.34 ± 0.079
4.682LeuGlu: 4.682 ± 0.071
3.164LeuPhe: 3.164 ± 0.061
8.955LeuGly: 8.955 ± 0.104
2.359LeuHis: 2.359 ± 0.04
4.087LeuIle: 4.087 ± 0.054
3.209LeuLys: 3.209 ± 0.049
11.749LeuLeu: 11.749 ± 0.151
2.603LeuMet: 2.603 ± 0.047
2.793LeuAsn: 2.793 ± 0.05
6.387LeuPro: 6.387 ± 0.076
6.218LeuGln: 6.218 ± 0.081
7.151LeuArg: 7.151 ± 0.081
6.412LeuSer: 6.412 ± 0.071
4.921LeuThr: 4.921 ± 0.065
8.164LeuVal: 8.164 ± 0.089
1.492LeuTrp: 1.492 ± 0.038
2.1LeuTyr: 2.1 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
3.548MetAla: 3.548 ± 0.05
0.168MetCys: 0.168 ± 0.011
1.254MetAsp: 1.254 ± 0.029
1.052MetGlu: 1.052 ± 0.027
0.711MetPhe: 0.711 ± 0.023
2.161MetGly: 2.161 ± 0.045
0.595MetHis: 0.595 ± 0.024
0.852MetIle: 0.852 ± 0.025
0.872MetLys: 0.872 ± 0.022
2.923MetLeu: 2.923 ± 0.047
0.555MetMet: 0.555 ± 0.022
0.76MetAsn: 0.76 ± 0.021
1.588MetPro: 1.588 ± 0.033
1.464MetGln: 1.464 ± 0.031
1.769MetArg: 1.769 ± 0.033
1.533MetSer: 1.533 ± 0.035
1.566MetThr: 1.566 ± 0.033
1.994MetVal: 1.994 ± 0.038
0.218MetTrp: 0.218 ± 0.012
0.395MetTyr: 0.395 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.459AsnAla: 3.459 ± 0.055
0.251AsnCys: 0.251 ± 0.013
1.339AsnAsp: 1.339 ± 0.033
1.12AsnGlu: 1.12 ± 0.031
0.939AsnPhe: 0.939 ± 0.026
2.39AsnGly: 2.39 ± 0.053
0.564AsnHis: 0.564 ± 0.021
1.257AsnIle: 1.257 ± 0.033
0.962AsnLys: 0.962 ± 0.029
2.682AsnLeu: 2.682 ± 0.044
0.603AsnMet: 0.603 ± 0.021
0.927AsnAsn: 0.927 ± 0.031
1.967AsnPro: 1.967 ± 0.059
1.138AsnGln: 1.138 ± 0.031
1.507AsnArg: 1.507 ± 0.035
1.447AsnSer: 1.447 ± 0.043
1.684AsnThr: 1.684 ± 0.04
1.902AsnVal: 1.902 ± 0.044
0.434AsnTrp: 0.434 ± 0.021
0.76AsnTyr: 0.76 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
7.711ProAla: 7.711 ± 0.109
0.349ProCys: 0.349 ± 0.018
2.907ProAsp: 2.907 ± 0.046
3.078ProGlu: 3.078 ± 0.047
1.686ProPhe: 1.686 ± 0.036
4.388ProGly: 4.388 ± 0.059
1.174ProHis: 1.174 ± 0.026
1.893ProIle: 1.893 ± 0.038
1.44ProLys: 1.44 ± 0.032
5.433ProLeu: 5.433 ± 0.072
1.312ProMet: 1.312 ± 0.035
1.26ProAsn: 1.26 ± 0.031
2.383ProPro: 2.383 ± 0.049
2.806ProGln: 2.806 ± 0.05
2.613ProArg: 2.613 ± 0.048
3.101ProSer: 3.101 ± 0.047
2.546ProThr: 2.546 ± 0.067
4.237ProVal: 4.237 ± 0.058
0.785ProTrp: 0.785 ± 0.022
1.22ProTyr: 1.22 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
7.752GlnAla: 7.752 ± 0.1
0.384GlnCys: 0.384 ± 0.017
2.113GlnAsp: 2.113 ± 0.042
2.048GlnGlu: 2.048 ± 0.041
1.416GlnPhe: 1.416 ± 0.03
4.026GlnGly: 4.026 ± 0.06
1.259GlnHis: 1.259 ± 0.034
2.108GlnIle: 2.108 ± 0.039
1.384GlnLys: 1.384 ± 0.032
5.897GlnLeu: 5.897 ± 0.074
1.243GlnMet: 1.243 ± 0.026
1.053GlnAsn: 1.053 ± 0.029
2.882GlnPro: 2.882 ± 0.047
3.098GlnGln: 3.098 ± 0.076
3.851GlnArg: 3.851 ± 0.063
2.655GlnSer: 2.655 ± 0.055
2.396GlnThr: 2.396 ± 0.042
3.736GlnVal: 3.736 ± 0.053
1.032GlnTrp: 1.032 ± 0.03
1.006GlnTyr: 1.006 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
6.709ArgAla: 6.709 ± 0.071
0.58ArgCys: 0.58 ± 0.021
3.019ArgAsp: 3.019 ± 0.053
3.251ArgGlu: 3.251 ± 0.066
2.434ArgPhe: 2.434 ± 0.047
3.793ArgGly: 3.793 ± 0.056
1.58ArgHis: 1.58 ± 0.039
3.189ArgIle: 3.189 ± 0.052
2.121ArgLys: 2.121 ± 0.042
6.716ArgLeu: 6.716 ± 0.081
1.979ArgMet: 1.979 ± 0.035
1.726ArgAsn: 1.726 ± 0.036
2.698ArgPro: 2.698 ± 0.044
3.161ArgGln: 3.161 ± 0.058
3.823ArgArg: 3.823 ± 0.059
3.664ArgSer: 3.664 ± 0.054
2.56ArgThr: 2.56 ± 0.049
4.128ArgVal: 4.128 ± 0.05
1.227ArgTrp: 1.227 ± 0.037
1.82ArgTyr: 1.82 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
7.305SerAla: 7.305 ± 0.087
0.521SerCys: 0.521 ± 0.023
2.853SerAsp: 2.853 ± 0.045
2.433SerGlu: 2.433 ± 0.036
2.178SerPhe: 2.178 ± 0.037
5.538SerGly: 5.538 ± 0.083
1.344SerHis: 1.344 ± 0.027
2.641SerIle: 2.641 ± 0.051
1.82SerLys: 1.82 ± 0.045
6.423SerLeu: 6.423 ± 0.064
1.418SerMet: 1.418 ± 0.028
1.77SerAsn: 1.77 ± 0.043
3.085SerPro: 3.085 ± 0.044
2.569SerGln: 2.569 ± 0.049
3.254SerArg: 3.254 ± 0.046
3.761SerSer: 3.761 ± 0.069
3.15SerThr: 3.15 ± 0.055
4.067SerVal: 4.067 ± 0.076
0.938SerTrp: 0.938 ± 0.026
1.543SerTyr: 1.543 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
6.351ThrAla: 6.351 ± 0.096
0.425ThrCys: 0.425 ± 0.03
2.414ThrAsp: 2.414 ± 0.044
2.253ThrGlu: 2.253 ± 0.047
1.502ThrPhe: 1.502 ± 0.035
4.581ThrGly: 4.581 ± 0.07
1.017ThrHis: 1.017 ± 0.027
2.214ThrIle: 2.214 ± 0.043
1.369ThrLys: 1.369 ± 0.037
5.335ThrLeu: 5.335 ± 0.062
1.148ThrMet: 1.148 ± 0.031
1.332ThrAsn: 1.332 ± 0.056
3.608ThrPro: 3.608 ± 0.058
2.239ThrGln: 2.239 ± 0.046
2.727ThrArg: 2.727 ± 0.047
2.957ThrSer: 2.957 ± 0.061
3.006ThrThr: 3.006 ± 0.075
4.366ThrVal: 4.366 ± 0.088
0.725ThrTrp: 0.725 ± 0.023
1.099ThrTyr: 1.099 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
9.345ValAla: 9.345 ± 0.094
0.758ValCys: 0.758 ± 0.024
3.917ValAsp: 3.917 ± 0.055
3.61ValGlu: 3.61 ± 0.063
2.628ValPhe: 2.628 ± 0.047
5.471ValGly: 5.471 ± 0.062
1.54ValHis: 1.54 ± 0.037
3.299ValIle: 3.299 ± 0.052
2.273ValLys: 2.273 ± 0.042
8.615ValLeu: 8.615 ± 0.09
1.986ValMet: 1.986 ± 0.036
2.134ValAsn: 2.134 ± 0.047
3.948ValPro: 3.948 ± 0.057
3.869ValGln: 3.869 ± 0.052
4.361ValArg: 4.361 ± 0.059
4.186ValSer: 4.186 ± 0.076
3.869ValThr: 3.869 ± 0.083
6.403ValVal: 6.403 ± 0.073
1.019ValTrp: 1.019 ± 0.028
1.612ValTyr: 1.612 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.522TrpAla: 1.522 ± 0.037
0.184TrpCys: 0.184 ± 0.01
0.675TrpAsp: 0.675 ± 0.024
0.537TrpGlu: 0.537 ± 0.019
0.538TrpPhe: 0.538 ± 0.019
1.134TrpGly: 1.134 ± 0.031
0.361TrpHis: 0.361 ± 0.016
0.666TrpIle: 0.666 ± 0.024
0.47TrpLys: 0.47 ± 0.018
2.264TrpLeu: 2.264 ± 0.05
0.474TrpMet: 0.474 ± 0.02
0.438TrpAsn: 0.438 ± 0.019
0.787TrpPro: 0.787 ± 0.025
1.084TrpGln: 1.084 ± 0.03
1.197TrpArg: 1.197 ± 0.034
0.927TrpSer: 0.927 ± 0.025
0.76TrpThr: 0.76 ± 0.025
1.074TrpVal: 1.074 ± 0.028
0.337TrpTrp: 0.337 ± 0.016
0.323TrpTyr: 0.323 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.819TyrAla: 2.819 ± 0.046
0.248TyrCys: 0.248 ± 0.012
1.279TyrAsp: 1.279 ± 0.039
1.139TyrGlu: 1.139 ± 0.027
0.909TyrPhe: 0.909 ± 0.024
2.046TyrGly: 2.046 ± 0.041
0.454TyrHis: 0.454 ± 0.019
0.841TyrIle: 0.841 ± 0.023
0.715TyrLys: 0.715 ± 0.023
2.432TyrLeu: 2.432 ± 0.043
0.456TyrMet: 0.456 ± 0.019
0.609TyrAsn: 0.609 ± 0.021
1.132TyrPro: 1.132 ± 0.027
1.186TyrGln: 1.186 ± 0.035
1.612TyrArg: 1.612 ± 0.034
1.386TyrSer: 1.386 ± 0.033
1.398TyrThr: 1.398 ± 0.039
1.6TyrVal: 1.6 ± 0.034
0.408TyrTrp: 0.408 ± 0.017
0.629TyrTyr: 0.629 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4372 proteins (1443950 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski