Amino acid dipepetide frequency for Clostridiales bacterium CHKCI001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.776AlaAla: 5.776 ± 0.111	1.024AlaCys: 1.024 ± 0.035	3.628AlaAsp: 3.628 ± 0.062	4.444AlaGlu: 4.444 ± 0.07	2.833AlaPhe: 2.833 ± 0.058	4.852AlaGly: 4.852 ± 0.089	0.999AlaHis: 0.999 ± 0.032	5.679AlaIle: 5.679 ± 0.085	4.546AlaLys: 4.546 ± 0.078	5.962AlaLeu: 5.962 ± 0.091	2.257AlaMet: 2.257 ± 0.049	2.702AlaAsn: 2.702 ± 0.053	1.718AlaPro: 1.718 ± 0.048	2.437AlaGln: 2.437 ± 0.054	2.405AlaArg: 2.405 ± 0.052	3.746AlaSer: 3.746 ± 0.07	2.978AlaThr: 2.978 ± 0.053	5.553AlaVal: 5.553 ± 0.084	0.587AlaTrp: 0.587 ± 0.025	2.547AlaTyr: 2.547 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.95CysAla: 0.95 ± 0.027	0.314CysCys: 0.314 ± 0.019	0.779CysAsp: 0.779 ± 0.031	0.986CysGlu: 0.986 ± 0.037	0.758CysPhe: 0.758 ± 0.03	1.344CysGly: 1.344 ± 0.046	0.312CysHis: 0.312 ± 0.018	1.343CysIle: 1.343 ± 0.042	0.871CysLys: 0.871 ± 0.031	1.166CysLeu: 1.166 ± 0.034	0.44CysMet: 0.44 ± 0.021	0.727CysAsn: 0.727 ± 0.027	0.631CysPro: 0.631 ± 0.028	0.486CysGln: 0.486 ± 0.022	0.552CysArg: 0.552 ± 0.025	0.903CysSer: 0.903 ± 0.032	0.723CysThr: 0.723 ± 0.028	0.975CysVal: 0.975 ± 0.039	0.124CysTrp: 0.124 ± 0.011	0.669CysTyr: 0.669 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	3.46AspAla: 3.46 ± 0.075	0.874AspCys: 0.874 ± 0.035	2.261AspAsp: 2.261 ± 0.061	3.862AspGlu: 3.862 ± 0.068	2.46AspPhe: 2.46 ± 0.045	4.052AspGly: 4.052 ± 0.092	0.893AspHis: 0.893 ± 0.033	4.176AspIle: 4.176 ± 0.074	3.034AspLys: 3.034 ± 0.055	4.5AspLeu: 4.5 ± 0.068	1.66AspMet: 1.66 ± 0.041	2.029AspAsn: 2.029 ± 0.047	1.73AspPro: 1.73 ± 0.043	1.715AspGln: 1.715 ± 0.045	2.085AspArg: 2.085 ± 0.051	2.768AspSer: 2.768 ± 0.05	2.914AspThr: 2.914 ± 0.059	3.363AspVal: 3.363 ± 0.065	0.66AspTrp: 0.66 ± 0.028	2.599AspTyr: 2.599 ± 0.053	0.0AspXaa: 0.0 ± 0.0
Glu	5.091GluAla: 5.091 ± 0.085	0.839GluCys: 0.839 ± 0.029	3.938GluAsp: 3.938 ± 0.067	7.621GluGlu: 7.621 ± 0.126	2.618GluPhe: 2.618 ± 0.05	4.077GluGly: 4.077 ± 0.075	1.334GluHis: 1.334 ± 0.045	5.901GluIle: 5.901 ± 0.091	6.702GluLys: 6.702 ± 0.101	6.734GluLeu: 6.734 ± 0.096	2.47GluMet: 2.47 ± 0.055	4.346GluAsn: 4.346 ± 0.074	1.72GluPro: 1.72 ± 0.046	3.811GluGln: 3.811 ± 0.083	3.333GluArg: 3.333 ± 0.071	3.445GluSer: 3.445 ± 0.077	3.988GluThr: 3.988 ± 0.086	4.433GluVal: 4.433 ± 0.084	0.822GluTrp: 0.822 ± 0.029	3.051GluTyr: 3.051 ± 0.058	0.0GluXaa: 0.0 ± 0.0
Phe	2.645PheAla: 2.645 ± 0.061	0.832PheCys: 0.832 ± 0.027	2.295PheAsp: 2.295 ± 0.049	2.604PheGlu: 2.604 ± 0.05	2.023PhePhe: 2.023 ± 0.05	3.127PheGly: 3.127 ± 0.06	0.957PheHis: 0.957 ± 0.031	3.315PheIle: 3.315 ± 0.073	1.815PheLys: 1.815 ± 0.039	4.334PheLeu: 4.334 ± 0.077	1.128PheMet: 1.128 ± 0.035	1.546PheAsn: 1.546 ± 0.038	1.43PhePro: 1.43 ± 0.036	1.734PheGln: 1.734 ± 0.045	1.559PheArg: 1.559 ± 0.042	3.068PheSer: 3.068 ± 0.067	2.181PheThr: 2.181 ± 0.056	2.857PheVal: 2.857 ± 0.059	0.512PheTrp: 0.512 ± 0.029	1.911PheTyr: 1.911 ± 0.051	0.0PheXaa: 0.0 ± 0.0
Gly	4.377GlyAla: 4.377 ± 0.087	1.156GlyCys: 1.156 ± 0.044	3.146GlyAsp: 3.146 ± 0.059	4.287GlyGlu: 4.287 ± 0.067	2.964GlyPhe: 2.964 ± 0.063	4.455GlyGly: 4.455 ± 0.089	1.124GlyHis: 1.124 ± 0.037	6.68GlyIle: 6.68 ± 0.094	5.323GlyLys: 5.323 ± 0.084	5.248GlyLeu: 5.248 ± 0.082	2.39GlyMet: 2.39 ± 0.043	3.155GlyAsn: 3.155 ± 0.07	1.124GlyPro: 1.124 ± 0.034	1.989GlyGln: 1.989 ± 0.048	2.648GlyArg: 2.648 ± 0.053	3.85GlySer: 3.85 ± 0.076	4.218GlyThr: 4.218 ± 0.079	4.747GlyVal: 4.747 ± 0.073	0.847GlyTrp: 0.847 ± 0.045	3.251GlyTyr: 3.251 ± 0.066	0.0GlyXaa: 0.0 ± 0.0
His	1.132HisAla: 1.132 ± 0.037	0.324HisCys: 0.324 ± 0.017	0.759HisAsp: 0.759 ± 0.033	0.964HisGlu: 0.964 ± 0.033	0.799HisPhe: 0.799 ± 0.027	1.224HisGly: 1.224 ± 0.037	0.444HisHis: 0.444 ± 0.024	1.562HisIle: 1.562 ± 0.04	0.999HisLys: 0.999 ± 0.035	1.591HisLeu: 1.591 ± 0.044	0.575HisMet: 0.575 ± 0.023	0.791HisAsn: 0.791 ± 0.03	0.905HisPro: 0.905 ± 0.033	0.655HisGln: 0.655 ± 0.026	0.817HisArg: 0.817 ± 0.027	1.089HisSer: 1.089 ± 0.034	1.018HisThr: 1.018 ± 0.033	1.133HisVal: 1.133 ± 0.036	0.214HisTrp: 0.214 ± 0.015	0.825HisTyr: 0.825 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.93IleAla: 5.93 ± 0.089	1.563IleCys: 1.563 ± 0.044	4.056IleAsp: 4.056 ± 0.062	5.512IleGlu: 5.512 ± 0.093	3.409IlePhe: 3.409 ± 0.079	6.01IleGly: 6.01 ± 0.106	1.673IleHis: 1.673 ± 0.037	6.457IleIle: 6.457 ± 0.097	4.532IleLys: 4.532 ± 0.067	8.144IleLeu: 8.144 ± 0.11	2.179IleMet: 2.179 ± 0.051	3.179IleAsn: 3.179 ± 0.059	3.526IlePro: 3.526 ± 0.062	3.576IleGln: 3.576 ± 0.061	3.752IleArg: 3.752 ± 0.066	5.271IleSer: 5.271 ± 0.087	4.611IleThr: 4.611 ± 0.077	5.71IleVal: 5.71 ± 0.094	0.815IleTrp: 0.815 ± 0.034	3.243IleTyr: 3.243 ± 0.061	0.0IleXaa: 0.0 ± 0.0
Lys	4.353LysAla: 4.353 ± 0.075	0.697LysCys: 0.697 ± 0.031	3.62LysAsp: 3.62 ± 0.067	7.344LysGlu: 7.344 ± 0.11	2.003LysPhe: 2.003 ± 0.045	3.952LysGly: 3.952 ± 0.069	1.018LysHis: 1.018 ± 0.034	5.453LysIle: 5.453 ± 0.087	7.053LysLys: 7.053 ± 0.119	5.553LysLeu: 5.553 ± 0.082	2.269LysMet: 2.269 ± 0.051	3.933LysAsn: 3.933 ± 0.071	1.956LysPro: 1.956 ± 0.046	3.223LysGln: 3.223 ± 0.074	3.289LysArg: 3.289 ± 0.065	3.22LysSer: 3.22 ± 0.063	3.716LysThr: 3.716 ± 0.058	3.959LysVal: 3.959 ± 0.075	0.681LysTrp: 0.681 ± 0.027	2.726LysTyr: 2.726 ± 0.057	0.0LysXaa: 0.0 ± 0.0
Leu	5.974LeuAla: 5.974 ± 0.082	1.49LeuCys: 1.49 ± 0.038	4.822LeuAsp: 4.822 ± 0.076	7.059LeuGlu: 7.059 ± 0.12	4.125LeuPhe: 4.125 ± 0.075	5.556LeuGly: 5.556 ± 0.083	1.596LeuHis: 1.596 ± 0.035	6.797LeuIle: 6.797 ± 0.11	6.369LeuLys: 6.369 ± 0.089	8.659LeuLeu: 8.659 ± 0.124	2.587LeuMet: 2.587 ± 0.049	4.236LeuAsn: 4.236 ± 0.069	3.339LeuPro: 3.339 ± 0.06	3.033LeuGln: 3.033 ± 0.065	3.488LeuArg: 3.488 ± 0.061	6.308LeuSer: 6.308 ± 0.104	5.012LeuThr: 5.012 ± 0.067	5.24LeuVal: 5.24 ± 0.083	0.855LeuTrp: 0.855 ± 0.032	3.435LeuTyr: 3.435 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	2.264MetAla: 2.264 ± 0.053	0.304MetCys: 0.304 ± 0.017	1.858MetAsp: 1.858 ± 0.047	2.81MetGlu: 2.81 ± 0.056	1.013MetPhe: 1.013 ± 0.029	2.003MetGly: 2.003 ± 0.046	0.454MetHis: 0.454 ± 0.02	2.488MetIle: 2.488 ± 0.053	2.747MetLys: 2.747 ± 0.053	2.672MetLeu: 2.672 ± 0.059	0.932MetMet: 0.932 ± 0.034	1.689MetAsn: 1.689 ± 0.041	1.061MetPro: 1.061 ± 0.032	1.131MetGln: 1.131 ± 0.031	1.309MetArg: 1.309 ± 0.041	1.77MetSer: 1.77 ± 0.043	1.649MetThr: 1.649 ± 0.046	1.777MetVal: 1.777 ± 0.038	0.238MetTrp: 0.238 ± 0.016	0.903MetTyr: 0.903 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	2.968AsnAla: 2.968 ± 0.059	0.744AsnCys: 0.744 ± 0.032	2.063AsnAsp: 2.063 ± 0.057	3.109AsnGlu: 3.109 ± 0.057	1.754AsnPhe: 1.754 ± 0.043	3.883AsnGly: 3.883 ± 0.082	0.969AsnHis: 0.969 ± 0.032	3.846AsnIle: 3.846 ± 0.072	2.8AsnLys: 2.8 ± 0.063	4.151AsnLeu: 4.151 ± 0.074	1.407AsnMet: 1.407 ± 0.042	2.094AsnAsn: 2.094 ± 0.059	2.067AsnPro: 2.067 ± 0.047	2.021AsnGln: 2.021 ± 0.043	2.209AsnArg: 2.209 ± 0.051	2.596AsnSer: 2.596 ± 0.058	2.551AsnThr: 2.551 ± 0.057	2.945AsnVal: 2.945 ± 0.056	0.573AsnTrp: 0.573 ± 0.025	2.209AsnTyr: 2.209 ± 0.05	0.0AsnXaa: 0.0 ± 0.0
Pro	1.994ProAla: 1.994 ± 0.052	0.452ProCys: 0.452 ± 0.022	1.983ProAsp: 1.983 ± 0.044	3.047ProGlu: 3.047 ± 0.07	1.574ProPhe: 1.574 ± 0.04	1.986ProGly: 1.986 ± 0.046	0.518ProHis: 0.518 ± 0.024	2.639ProIle: 2.639 ± 0.05	1.918ProLys: 1.918 ± 0.051	2.723ProLeu: 2.723 ± 0.051	0.877ProMet: 0.877 ± 0.031	1.502ProAsn: 1.502 ± 0.04	0.65ProPro: 0.65 ± 0.027	1.029ProGln: 1.029 ± 0.035	0.917ProArg: 0.917 ± 0.033	1.942ProSer: 1.942 ± 0.043	1.636ProThr: 1.636 ± 0.041	2.625ProVal: 2.625 ± 0.054	0.321ProTrp: 0.321 ± 0.019	1.461ProTyr: 1.461 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.466GlnAla: 2.466 ± 0.057	0.392GlnCys: 0.392 ± 0.021	1.633GlnAsp: 1.633 ± 0.05	3.193GlnGlu: 3.193 ± 0.066	1.534GlnPhe: 1.534 ± 0.043	1.838GlnGly: 1.838 ± 0.044	0.536GlnHis: 0.536 ± 0.024	3.471GlnIle: 3.471 ± 0.07	3.292GlnLys: 3.292 ± 0.068	3.677GlnLeu: 3.677 ± 0.069	1.488GlnMet: 1.488 ± 0.044	2.078GlnAsn: 2.078 ± 0.063	0.983GlnPro: 0.983 ± 0.035	1.533GlnGln: 1.533 ± 0.046	1.622GlnArg: 1.622 ± 0.04	1.887GlnSer: 1.887 ± 0.043	2.183GlnThr: 2.183 ± 0.056	2.415GlnVal: 2.415 ± 0.048	0.415GlnTrp: 0.415 ± 0.023	1.732GlnTyr: 1.732 ± 0.048	0.0GlnXaa: 0.0 ± 0.0
Arg	2.271ArgAla: 2.271 ± 0.049	0.588ArgCys: 0.588 ± 0.028	1.887ArgAsp: 1.887 ± 0.051	3.322ArgGlu: 3.322 ± 0.064	1.744ArgPhe: 1.744 ± 0.043	2.197ArgGly: 2.197 ± 0.053	0.664ArgHis: 0.664 ± 0.028	3.611ArgIle: 3.611 ± 0.061	3.441ArgLys: 3.441 ± 0.072	3.777ArgLeu: 3.777 ± 0.074	1.528ArgMet: 1.528 ± 0.044	2.111ArgAsn: 2.111 ± 0.039	1.195ArgPro: 1.195 ± 0.039	1.52ArgGln: 1.52 ± 0.041	1.902ArgArg: 1.902 ± 0.048	2.126ArgSer: 2.126 ± 0.049	2.095ArgThr: 2.095 ± 0.048	2.512ArgVal: 2.512 ± 0.055	0.403ArgTrp: 0.403 ± 0.022	1.822ArgTyr: 1.822 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	3.607SerAla: 3.607 ± 0.059	0.842SerCys: 0.842 ± 0.031	3.101SerAsp: 3.101 ± 0.063	4.083SerGlu: 4.083 ± 0.072	2.779SerPhe: 2.779 ± 0.058	4.515SerGly: 4.515 ± 0.081	1.027SerHis: 1.027 ± 0.033	4.893SerIle: 4.893 ± 0.09	3.527SerLys: 3.527 ± 0.055	5.205SerLeu: 5.205 ± 0.079	1.81SerMet: 1.81 ± 0.047	2.862SerAsn: 2.862 ± 0.062	1.69SerPro: 1.69 ± 0.042	2.111SerGln: 2.111 ± 0.048	2.24SerArg: 2.24 ± 0.046	3.877SerSer: 3.877 ± 0.083	2.882SerThr: 2.882 ± 0.067	4.045SerVal: 4.045 ± 0.067	0.596SerTrp: 0.596 ± 0.029	2.732SerTyr: 2.732 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	3.916ThrAla: 3.916 ± 0.077	0.611ThrCys: 0.611 ± 0.023	2.819ThrAsp: 2.819 ± 0.061	3.694ThrGlu: 3.694 ± 0.067	2.147ThrPhe: 2.147 ± 0.05	4.175ThrGly: 4.175 ± 0.077	1.028ThrHis: 1.028 ± 0.04	4.92ThrIle: 4.92 ± 0.084	3.394ThrLys: 3.394 ± 0.062	4.666ThrLeu: 4.666 ± 0.079	1.566ThrMet: 1.566 ± 0.037	2.403ThrAsn: 2.403 ± 0.047	2.033ThrPro: 2.033 ± 0.049	1.74ThrGln: 1.74 ± 0.04	1.716ThrArg: 1.716 ± 0.038	3.132ThrSer: 3.132 ± 0.065	3.151ThrThr: 3.151 ± 0.08	4.424ThrVal: 4.424 ± 0.085	0.558ThrTrp: 0.558 ± 0.028	2.236ThrTyr: 2.236 ± 0.082	0.0ThrXaa: 0.0 ± 0.0
Val	4.422ValAla: 4.422 ± 0.068	1.136ValCys: 1.136 ± 0.028	3.509ValAsp: 3.509 ± 0.067	4.378ValGlu: 4.378 ± 0.08	2.924ValPhe: 2.924 ± 0.057	4.146ValGly: 4.146 ± 0.073	1.083ValHis: 1.083 ± 0.032	5.678ValIle: 5.678 ± 0.09	4.297ValLys: 4.297 ± 0.076	6.55ValLeu: 6.55 ± 0.091	1.954ValMet: 1.954 ± 0.039	2.915ValAsn: 2.915 ± 0.057	2.308ValPro: 2.308 ± 0.048	2.324ValGln: 2.324 ± 0.052	2.459ValArg: 2.459 ± 0.048	4.342ValSer: 4.342 ± 0.067	4.091ValThr: 4.091 ± 0.077	4.65ValVal: 4.65 ± 0.072	0.662ValTrp: 0.662 ± 0.024	2.576ValTyr: 2.576 ± 0.052	0.0ValXaa: 0.0 ± 0.0
Trp	0.513TrpAla: 0.513 ± 0.024	0.155TrpCys: 0.155 ± 0.013	0.609TrpAsp: 0.609 ± 0.028	0.77TrpGlu: 0.77 ± 0.031	0.442TrpPhe: 0.442 ± 0.025	0.677TrpGly: 0.677 ± 0.026	0.19TrpHis: 0.19 ± 0.015	0.979TrpIle: 0.979 ± 0.036	0.987TrpLys: 0.987 ± 0.03	0.907TrpLeu: 0.907 ± 0.032	0.42TrpMet: 0.42 ± 0.021	0.727TrpAsn: 0.727 ± 0.029	0.196TrpPro: 0.196 ± 0.016	0.42TrpGln: 0.42 ± 0.03	0.356TrpArg: 0.356 ± 0.018	0.551TrpSer: 0.551 ± 0.025	0.489TrpThr: 0.489 ± 0.022	0.53TrpVal: 0.53 ± 0.026	0.142TrpTrp: 0.142 ± 0.012	0.427TrpTyr: 0.427 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.567TyrAla: 2.567 ± 0.055	0.713TyrCys: 0.713 ± 0.025	2.33TyrAsp: 2.33 ± 0.063	3.185TyrGlu: 3.185 ± 0.063	1.971TyrPhe: 1.971 ± 0.039	2.91TyrGly: 2.91 ± 0.053	1.001TyrHis: 1.001 ± 0.033	3.259TyrIle: 3.259 ± 0.057	2.376TyrLys: 2.376 ± 0.06	3.762TyrLeu: 3.762 ± 0.068	1.132TyrMet: 1.132 ± 0.033	1.935TyrAsn: 1.935 ± 0.044	1.533TyrPro: 1.533 ± 0.041	1.876TyrGln: 1.876 ± 0.043	2.057TyrArg: 2.057 ± 0.052	2.532TyrSer: 2.532 ± 0.057	2.243TyrThr: 2.243 ± 0.058	2.513TyrVal: 2.513 ± 0.048	0.463TyrTrp: 0.463 ± 0.022	2.174TyrTyr: 2.174 ± 0.054	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3078 proteins (979425 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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