Amino acid dipepetide frequency for Mycobacterium phage ThetaBob

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.718AlaAla: 12.718 ± 1.397
1.261AlaCys: 1.261 ± 0.304
6.14AlaAsp: 6.14 ± 0.502
8.168AlaGlu: 8.168 ± 0.946
2.905AlaPhe: 2.905 ± 0.405
7.565AlaGly: 7.565 ± 1.176
2.412AlaHis: 2.412 ± 0.538
4.385AlaIle: 4.385 ± 0.534
4.166AlaLys: 4.166 ± 0.45
9.758AlaLeu: 9.758 ± 0.766
2.796AlaMet: 2.796 ± 0.448
3.454AlaAsn: 3.454 ± 0.604
5.372AlaPro: 5.372 ± 0.733
3.837AlaGln: 3.837 ± 0.415
7.181AlaArg: 7.181 ± 0.675
5.263AlaSer: 5.263 ± 0.617
5.153AlaThr: 5.153 ± 0.615
7.784AlaVal: 7.784 ± 0.553
2.96AlaTrp: 2.96 ± 0.584
2.028AlaTyr: 2.028 ± 0.367
0.0AlaXaa: 0.0 ± 0.0
Cys
1.042CysAla: 1.042 ± 0.273
0.11CysCys: 0.11 ± 0.078
0.987CysAsp: 0.987 ± 0.26
0.603CysGlu: 0.603 ± 0.198
0.11CysPhe: 0.11 ± 0.086
1.261CysGly: 1.261 ± 0.296
0.329CysHis: 0.329 ± 0.157
0.384CysIle: 0.384 ± 0.136
0.384CysLys: 0.384 ± 0.157
0.822CysLeu: 0.822 ± 0.252
0.11CysMet: 0.11 ± 0.071
0.274CysAsn: 0.274 ± 0.118
0.987CysPro: 0.987 ± 0.29
0.329CysGln: 0.329 ± 0.157
1.151CysArg: 1.151 ± 0.264
0.713CysSer: 0.713 ± 0.213
0.987CysThr: 0.987 ± 0.287
0.713CysVal: 0.713 ± 0.204
0.274CysTrp: 0.274 ± 0.122
0.219CysTyr: 0.219 ± 0.099
0.0CysXaa: 0.0 ± 0.0
Asp
6.688AspAla: 6.688 ± 0.639
0.603AspCys: 0.603 ± 0.17
4.166AspAsp: 4.166 ± 0.585
3.892AspGlu: 3.892 ± 0.512
1.206AspPhe: 1.206 ± 0.243
5.811AspGly: 5.811 ± 0.578
1.316AspHis: 1.316 ± 0.279
1.973AspIle: 1.973 ± 0.254
2.028AspLys: 2.028 ± 0.34
4.824AspLeu: 4.824 ± 0.453
1.151AspMet: 1.151 ± 0.249
1.535AspAsn: 1.535 ± 0.301
4.221AspPro: 4.221 ± 0.498
2.631AspGln: 2.631 ± 0.375
4.276AspArg: 4.276 ± 0.459
3.234AspSer: 3.234 ± 0.431
3.673AspThr: 3.673 ± 0.447
4.824AspVal: 4.824 ± 0.52
1.37AspTrp: 1.37 ± 0.274
1.809AspTyr: 1.809 ± 0.376
0.0AspXaa: 0.0 ± 0.0
Glu
6.852GluAla: 6.852 ± 1.013
1.042GluCys: 1.042 ± 0.27
3.179GluAsp: 3.179 ± 0.37
3.234GluGlu: 3.234 ± 0.516
2.522GluPhe: 2.522 ± 0.315
3.234GluGly: 3.234 ± 0.529
1.316GluHis: 1.316 ± 0.292
2.083GluIle: 2.083 ± 0.364
1.864GluLys: 1.864 ± 0.323
5.153GluLeu: 5.153 ± 0.541
1.48GluMet: 1.48 ± 0.289
2.248GluAsn: 2.248 ± 0.332
2.905GluPro: 2.905 ± 0.321
3.563GluGln: 3.563 ± 0.449
4.934GluArg: 4.934 ± 0.629
2.741GluSer: 2.741 ± 0.371
3.234GluThr: 3.234 ± 0.395
5.153GluVal: 5.153 ± 0.5
1.425GluTrp: 1.425 ± 0.239
2.028GluTyr: 2.028 ± 0.384
0.0GluXaa: 0.0 ± 0.0
Phe
3.125PheAla: 3.125 ± 0.482
0.329PheCys: 0.329 ± 0.131
2.467PheAsp: 2.467 ± 0.425
1.864PheGlu: 1.864 ± 0.303
0.493PhePhe: 0.493 ± 0.147
3.07PheGly: 3.07 ± 0.424
0.603PheHis: 0.603 ± 0.181
1.37PheIle: 1.37 ± 0.277
0.658PheLys: 0.658 ± 0.159
2.686PheLeu: 2.686 ± 0.461
0.603PheMet: 0.603 ± 0.167
1.042PheAsn: 1.042 ± 0.249
1.754PhePro: 1.754 ± 0.398
1.042PheGln: 1.042 ± 0.294
1.864PheArg: 1.864 ± 0.322
1.151PheSer: 1.151 ± 0.237
2.796PheThr: 2.796 ± 0.343
1.535PheVal: 1.535 ± 0.281
0.329PheTrp: 0.329 ± 0.139
0.713PheTyr: 0.713 ± 0.204
0.0PheXaa: 0.0 ± 0.0
Gly
8.771GlyAla: 8.771 ± 1.088
0.932GlyCys: 0.932 ± 0.202
3.947GlyAsp: 3.947 ± 0.404
4.331GlyGlu: 4.331 ± 0.491
2.576GlyPhe: 2.576 ± 0.404
10.69GlyGly: 10.69 ± 2.044
1.864GlyHis: 1.864 ± 0.39
3.782GlyIle: 3.782 ± 0.569
3.673GlyLys: 3.673 ± 0.438
5.701GlyLeu: 5.701 ± 0.685
1.809GlyMet: 1.809 ± 0.368
3.289GlyAsn: 3.289 ± 0.501
3.947GlyPro: 3.947 ± 0.395
2.851GlyGln: 2.851 ± 0.538
6.14GlyArg: 6.14 ± 0.68
5.427GlySer: 5.427 ± 1.005
5.866GlyThr: 5.866 ± 0.721
6.359GlyVal: 6.359 ± 0.722
2.576GlyTrp: 2.576 ± 0.394
2.302GlyTyr: 2.302 ± 0.355
0.0GlyXaa: 0.0 ± 0.0
His
2.028HisAla: 2.028 ± 0.305
0.493HisCys: 0.493 ± 0.178
0.987HisAsp: 0.987 ± 0.235
0.877HisGlu: 0.877 ± 0.228
0.384HisPhe: 0.384 ± 0.157
1.699HisGly: 1.699 ± 0.314
0.767HisHis: 0.767 ± 0.246
0.877HisIle: 0.877 ± 0.228
0.713HisLys: 0.713 ± 0.196
1.809HisLeu: 1.809 ± 0.345
0.274HisMet: 0.274 ± 0.099
0.932HisAsn: 0.932 ± 0.198
0.987HisPro: 0.987 ± 0.209
0.822HisGln: 0.822 ± 0.25
2.138HisArg: 2.138 ± 0.351
1.261HisSer: 1.261 ± 0.269
1.535HisThr: 1.535 ± 0.401
1.425HisVal: 1.425 ± 0.452
0.822HisTrp: 0.822 ± 0.207
1.042HisTyr: 1.042 ± 0.265
0.0HisXaa: 0.0 ± 0.0
Ile
5.208IleAla: 5.208 ± 0.645
0.767IleCys: 0.767 ± 0.234
3.892IleAsp: 3.892 ± 0.397
3.234IleGlu: 3.234 ± 0.446
1.042IlePhe: 1.042 ± 0.281
3.618IleGly: 3.618 ± 0.466
0.987IleHis: 0.987 ± 0.237
1.37IleIle: 1.37 ± 0.241
1.096IleLys: 1.096 ± 0.237
2.467IleLeu: 2.467 ± 0.347
0.987IleMet: 0.987 ± 0.221
1.754IleAsn: 1.754 ± 0.315
2.741IlePro: 2.741 ± 0.43
1.316IleGln: 1.316 ± 0.275
2.357IleArg: 2.357 ± 0.367
1.809IleSer: 1.809 ± 0.307
3.234IleThr: 3.234 ± 0.513
2.905IleVal: 2.905 ± 0.447
0.822IleTrp: 0.822 ± 0.242
1.151IleTyr: 1.151 ± 0.189
0.0IleXaa: 0.0 ± 0.0
Lys
3.508LysAla: 3.508 ± 0.519
0.164LysCys: 0.164 ± 0.099
1.864LysAsp: 1.864 ± 0.347
1.59LysGlu: 1.59 ± 0.346
1.096LysPhe: 1.096 ± 0.254
2.686LysGly: 2.686 ± 0.358
1.151LysHis: 1.151 ± 0.242
1.37LysIle: 1.37 ± 0.289
1.151LysLys: 1.151 ± 0.325
2.741LysLeu: 2.741 ± 0.425
0.658LysMet: 0.658 ± 0.157
0.493LysAsn: 0.493 ± 0.16
2.686LysPro: 2.686 ± 0.46
1.206LysGln: 1.206 ± 0.272
3.179LysArg: 3.179 ± 0.39
1.809LysSer: 1.809 ± 0.354
2.028LysThr: 2.028 ± 0.379
2.905LysVal: 2.905 ± 0.399
0.822LysTrp: 0.822 ± 0.231
0.987LysTyr: 0.987 ± 0.221
0.0LysXaa: 0.0 ± 0.0
Leu
8.826LeuAla: 8.826 ± 0.724
0.603LeuCys: 0.603 ± 0.23
4.44LeuAsp: 4.44 ± 0.547
4.276LeuGlu: 4.276 ± 0.516
1.973LeuPhe: 1.973 ± 0.304
6.852LeuGly: 6.852 ± 0.662
1.48LeuHis: 1.48 ± 0.353
3.673LeuIle: 3.673 ± 0.488
2.905LeuLys: 2.905 ± 0.371
6.743LeuLeu: 6.743 ± 0.617
1.535LeuMet: 1.535 ± 0.299
3.234LeuAsn: 3.234 ± 0.411
5.482LeuPro: 5.482 ± 0.58
2.522LeuGln: 2.522 ± 0.423
5.153LeuArg: 5.153 ± 0.547
4.605LeuSer: 4.605 ± 0.502
4.988LeuThr: 4.988 ± 0.588
5.317LeuVal: 5.317 ± 0.584
1.316LeuTrp: 1.316 ± 0.326
1.864LeuTyr: 1.864 ± 0.398
0.0LeuXaa: 0.0 ± 0.0
Met
2.028MetAla: 2.028 ± 0.29
0.219MetCys: 0.219 ± 0.114
0.713MetAsp: 0.713 ± 0.232
1.316MetGlu: 1.316 ± 0.26
1.206MetPhe: 1.206 ± 0.282
1.535MetGly: 1.535 ± 0.528
0.329MetHis: 0.329 ± 0.132
1.096MetIle: 1.096 ± 0.216
0.439MetLys: 0.439 ± 0.153
1.206MetLeu: 1.206 ± 0.268
0.439MetMet: 0.439 ± 0.131
1.151MetAsn: 1.151 ± 0.226
1.261MetPro: 1.261 ± 0.246
0.493MetGln: 0.493 ± 0.169
0.987MetArg: 0.987 ± 0.226
2.522MetSer: 2.522 ± 0.299
2.631MetThr: 2.631 ± 0.347
1.535MetVal: 1.535 ± 0.293
0.658MetTrp: 0.658 ± 0.162
0.329MetTyr: 0.329 ± 0.125
0.0MetXaa: 0.0 ± 0.0
Asn
3.563AsnAla: 3.563 ± 0.42
0.164AsnCys: 0.164 ± 0.098
1.864AsnAsp: 1.864 ± 0.301
2.302AsnGlu: 2.302 ± 0.324
0.658AsnPhe: 0.658 ± 0.153
3.782AsnGly: 3.782 ± 0.397
1.096AsnHis: 1.096 ± 0.279
1.535AsnIle: 1.535 ± 0.279
0.713AsnLys: 0.713 ± 0.165
2.631AsnLeu: 2.631 ± 0.467
0.384AsnMet: 0.384 ± 0.137
1.261AsnAsn: 1.261 ± 0.248
2.851AsnPro: 2.851 ± 0.455
1.261AsnGln: 1.261 ± 0.338
2.631AsnArg: 2.631 ± 0.436
1.59AsnSer: 1.59 ± 0.267
2.796AsnThr: 2.796 ± 0.439
1.809AsnVal: 1.809 ± 0.271
0.548AsnTrp: 0.548 ± 0.156
0.658AsnTyr: 0.658 ± 0.171
0.0AsnXaa: 0.0 ± 0.0
Pro
5.646ProAla: 5.646 ± 0.637
0.658ProCys: 0.658 ± 0.202
3.07ProAsp: 3.07 ± 0.39
4.221ProGlu: 4.221 ± 0.574
1.973ProPhe: 1.973 ± 0.32
6.03ProGly: 6.03 ± 0.693
1.316ProHis: 1.316 ± 0.293
2.138ProIle: 2.138 ± 0.358
2.138ProLys: 2.138 ± 0.416
3.399ProLeu: 3.399 ± 0.361
1.48ProMet: 1.48 ± 0.363
2.686ProAsn: 2.686 ± 0.423
4.166ProPro: 4.166 ± 0.48
2.851ProGln: 2.851 ± 0.538
2.522ProArg: 2.522 ± 0.322
3.289ProSer: 3.289 ± 0.383
4.221ProThr: 4.221 ± 0.633
5.098ProVal: 5.098 ± 0.612
1.316ProTrp: 1.316 ± 0.287
1.919ProTyr: 1.919 ± 0.288
0.0ProXaa: 0.0 ± 0.0
Gln
4.44GlnAla: 4.44 ± 0.547
0.329GlnCys: 0.329 ± 0.136
2.083GlnAsp: 2.083 ± 0.296
1.206GlnGlu: 1.206 ± 0.309
1.316GlnPhe: 1.316 ± 0.241
2.96GlnGly: 2.96 ± 0.481
1.042GlnHis: 1.042 ± 0.225
2.028GlnIle: 2.028 ± 0.307
1.59GlnLys: 1.59 ± 0.256
3.508GlnLeu: 3.508 ± 0.564
1.096GlnMet: 1.096 ± 0.233
0.767GlnAsn: 0.767 ± 0.25
2.851GlnPro: 2.851 ± 0.535
1.754GlnGln: 1.754 ± 0.464
2.467GlnArg: 2.467 ± 0.374
2.138GlnSer: 2.138 ± 0.343
1.864GlnThr: 1.864 ± 0.333
2.741GlnVal: 2.741 ± 0.341
0.439GlnTrp: 0.439 ± 0.162
0.822GlnTyr: 0.822 ± 0.155
0.0GlnXaa: 0.0 ± 0.0
Arg
6.578ArgAla: 6.578 ± 0.696
1.206ArgCys: 1.206 ± 0.309
3.618ArgAsp: 3.618 ± 0.495
4.44ArgGlu: 4.44 ± 0.53
2.796ArgPhe: 2.796 ± 0.384
4.276ArgGly: 4.276 ± 0.401
1.48ArgHis: 1.48 ± 0.288
3.782ArgIle: 3.782 ± 0.514
2.851ArgLys: 2.851 ± 0.59
6.03ArgLeu: 6.03 ± 0.531
2.028ArgMet: 2.028 ± 0.302
2.467ArgAsn: 2.467 ± 0.328
3.728ArgPro: 3.728 ± 0.485
2.686ArgGln: 2.686 ± 0.423
5.537ArgArg: 5.537 ± 0.721
4.331ArgSer: 4.331 ± 0.428
3.07ArgThr: 3.07 ± 0.48
5.756ArgVal: 5.756 ± 0.642
1.48ArgTrp: 1.48 ± 0.283
2.028ArgTyr: 2.028 ± 0.342
0.0ArgXaa: 0.0 ± 0.0
Ser
6.14SerAla: 6.14 ± 0.665
0.493SerCys: 0.493 ± 0.147
4.385SerAsp: 4.385 ± 0.463
2.686SerGlu: 2.686 ± 0.364
1.645SerPhe: 1.645 ± 0.31
6.249SerGly: 6.249 ± 0.971
0.877SerHis: 0.877 ± 0.242
2.522SerIle: 2.522 ± 0.304
2.193SerLys: 2.193 ± 0.402
4.331SerLeu: 4.331 ± 0.468
1.699SerMet: 1.699 ± 0.302
1.535SerAsn: 1.535 ± 0.282
3.618SerPro: 3.618 ± 0.351
1.919SerGln: 1.919 ± 0.312
3.179SerArg: 3.179 ± 0.554
4.166SerSer: 4.166 ± 0.593
3.454SerThr: 3.454 ± 0.388
3.618SerVal: 3.618 ± 0.546
0.987SerTrp: 0.987 ± 0.246
1.042SerTyr: 1.042 ± 0.238
0.0SerXaa: 0.0 ± 0.0
Thr
5.537ThrAla: 5.537 ± 0.621
0.493ThrCys: 0.493 ± 0.19
4.057ThrAsp: 4.057 ± 0.473
3.892ThrGlu: 3.892 ± 0.482
2.028ThrPhe: 2.028 ± 0.32
6.085ThrGly: 6.085 ± 0.78
0.932ThrHis: 0.932 ± 0.222
3.07ThrIle: 3.07 ± 0.446
1.261ThrLys: 1.261 ± 0.302
4.714ThrLeu: 4.714 ± 0.564
1.37ThrMet: 1.37 ± 0.268
2.248ThrAsn: 2.248 ± 0.285
4.824ThrPro: 4.824 ± 0.489
2.412ThrGln: 2.412 ± 0.559
4.385ThrArg: 4.385 ± 0.531
3.618ThrSer: 3.618 ± 0.522
4.605ThrThr: 4.605 ± 0.608
6.359ThrVal: 6.359 ± 0.57
1.425ThrTrp: 1.425 ± 0.28
1.37ThrTyr: 1.37 ± 0.256
0.0ThrXaa: 0.0 ± 0.0
Val
8.442ValAla: 8.442 ± 0.694
1.042ValCys: 1.042 ± 0.251
5.537ValAsp: 5.537 ± 0.52
5.756ValGlu: 5.756 ± 0.866
2.302ValPhe: 2.302 ± 0.324
5.811ValGly: 5.811 ± 0.53
1.37ValHis: 1.37 ± 0.248
3.728ValIle: 3.728 ± 0.413
2.522ValLys: 2.522 ± 0.317
5.866ValLeu: 5.866 ± 0.619
1.096ValMet: 1.096 ± 0.291
2.302ValAsn: 2.302 ± 0.326
3.728ValPro: 3.728 ± 0.405
2.741ValGln: 2.741 ± 0.344
5.208ValArg: 5.208 ± 0.613
4.276ValSer: 4.276 ± 0.81
4.55ValThr: 4.55 ± 0.524
6.304ValVal: 6.304 ± 0.768
1.59ValTrp: 1.59 ± 0.253
1.48ValTyr: 1.48 ± 0.299
0.0ValXaa: 0.0 ± 0.0
Trp
1.535TrpAla: 1.535 ± 0.307
0.603TrpCys: 0.603 ± 0.195
1.919TrpAsp: 1.919 ± 0.29
0.822TrpGlu: 0.822 ± 0.289
0.603TrpPhe: 0.603 ± 0.161
1.425TrpGly: 1.425 ± 0.431
0.329TrpHis: 0.329 ± 0.132
0.713TrpIle: 0.713 ± 0.178
1.206TrpLys: 1.206 ± 0.246
1.754TrpLeu: 1.754 ± 0.281
0.658TrpMet: 0.658 ± 0.194
0.603TrpAsn: 0.603 ± 0.181
1.042TrpPro: 1.042 ± 0.226
0.548TrpGln: 0.548 ± 0.172
1.699TrpArg: 1.699 ± 0.337
1.535TrpSer: 1.535 ± 0.3
2.357TrpThr: 2.357 ± 0.42
1.754TrpVal: 1.754 ± 0.309
0.877TrpTrp: 0.877 ± 0.238
0.603TrpTyr: 0.603 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.631TyrAla: 2.631 ± 0.363
0.274TyrCys: 0.274 ± 0.108
1.919TyrAsp: 1.919 ± 0.293
1.48TyrGlu: 1.48 ± 0.318
0.658TyrPhe: 0.658 ± 0.224
2.193TyrGly: 2.193 ± 0.329
0.877TyrHis: 0.877 ± 0.181
0.877TyrIle: 0.877 ± 0.262
0.548TyrLys: 0.548 ± 0.152
1.645TyrLeu: 1.645 ± 0.275
0.384TyrMet: 0.384 ± 0.144
0.822TyrAsn: 0.822 ± 0.242
0.987TyrPro: 0.987 ± 0.244
0.658TyrGln: 0.658 ± 0.173
3.179TyrArg: 3.179 ± 0.479
1.261TyrSer: 1.261 ± 0.222
1.699TyrThr: 1.699 ± 0.268
1.754TyrVal: 1.754 ± 0.321
0.548TyrTrp: 0.548 ± 0.201
0.658TyrTyr: 0.658 ± 0.221
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 106 proteins (18243 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski