Amino acid dipepetide frequency for Salmonella phage SI8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.533AlaAla: 8.533 ± 1.344
1.171AlaCys: 1.171 ± 0.287
5.772AlaAsp: 5.772 ± 0.741
7.445AlaGlu: 7.445 ± 0.789
3.681AlaPhe: 3.681 ± 0.566
6.943AlaGly: 6.943 ± 0.878
0.753AlaHis: 0.753 ± 0.286
5.521AlaIle: 5.521 ± 0.802
5.438AlaLys: 5.438 ± 0.765
7.362AlaLeu: 7.362 ± 0.855
3.43AlaMet: 3.43 ± 0.547
5.187AlaAsn: 5.187 ± 0.796
1.757AlaPro: 1.757 ± 0.373
3.932AlaGln: 3.932 ± 0.543
5.605AlaArg: 5.605 ± 0.597
4.266AlaSer: 4.266 ± 0.654
5.438AlaThr: 5.438 ± 0.835
4.601AlaVal: 4.601 ± 0.634
1.757AlaTrp: 1.757 ± 0.377
2.175AlaTyr: 2.175 ± 0.455
0.0AlaXaa: 0.0 ± 0.0
Cys
1.255CysAla: 1.255 ± 0.404
0.418CysCys: 0.418 ± 0.175
0.586CysAsp: 0.586 ± 0.254
1.004CysGlu: 1.004 ± 0.335
0.335CysPhe: 0.335 ± 0.165
1.171CysGly: 1.171 ± 0.3
0.167CysHis: 0.167 ± 0.133
0.502CysIle: 0.502 ± 0.289
0.669CysLys: 0.669 ± 0.249
0.418CysLeu: 0.418 ± 0.215
0.167CysMet: 0.167 ± 0.126
0.669CysAsn: 0.669 ± 0.283
0.418CysPro: 0.418 ± 0.18
0.418CysGln: 0.418 ± 0.173
1.004CysArg: 1.004 ± 0.305
0.753CysSer: 0.753 ± 0.252
0.586CysThr: 0.586 ± 0.212
0.502CysVal: 0.502 ± 0.165
0.167CysTrp: 0.167 ± 0.124
0.335CysTyr: 0.335 ± 0.138
0.0CysXaa: 0.0 ± 0.0
Asp
6.943AspAla: 6.943 ± 0.914
0.502AspCys: 0.502 ± 0.206
4.35AspAsp: 4.35 ± 0.649
4.517AspGlu: 4.517 ± 0.592
2.008AspPhe: 2.008 ± 0.361
5.772AspGly: 5.772 ± 0.721
1.088AspHis: 1.088 ± 0.32
4.517AspIle: 4.517 ± 0.793
3.012AspLys: 3.012 ± 0.479
4.015AspLeu: 4.015 ± 0.66
1.422AspMet: 1.422 ± 0.406
2.844AspAsn: 2.844 ± 0.532
1.589AspPro: 1.589 ± 0.361
1.255AspGln: 1.255 ± 0.282
2.175AspArg: 2.175 ± 0.408
2.761AspSer: 2.761 ± 0.402
1.589AspThr: 1.589 ± 0.449
4.936AspVal: 4.936 ± 0.597
1.088AspTrp: 1.088 ± 0.33
2.928AspTyr: 2.928 ± 0.376
0.0AspXaa: 0.0 ± 0.0
Glu
5.019GluAla: 5.019 ± 0.627
0.753GluCys: 0.753 ± 0.206
3.263GluAsp: 3.263 ± 0.528
4.936GluGlu: 4.936 ± 0.936
3.848GluPhe: 3.848 ± 0.507
3.597GluGly: 3.597 ± 0.698
1.338GluHis: 1.338 ± 0.255
4.936GluIle: 4.936 ± 0.745
4.183GluLys: 4.183 ± 0.595
6.023GluLeu: 6.023 ± 0.844
1.84GluMet: 1.84 ± 0.466
3.43GluAsn: 3.43 ± 0.586
2.51GluPro: 2.51 ± 0.409
3.848GluGln: 3.848 ± 0.625
4.266GluArg: 4.266 ± 0.643
3.848GluSer: 3.848 ± 0.448
3.179GluThr: 3.179 ± 0.539
3.932GluVal: 3.932 ± 0.553
1.673GluTrp: 1.673 ± 0.365
2.426GluTyr: 2.426 ± 0.525
0.0GluXaa: 0.0 ± 0.0
Phe
3.513PheAla: 3.513 ± 0.657
0.167PheCys: 0.167 ± 0.113
2.175PheAsp: 2.175 ± 0.391
1.589PheGlu: 1.589 ± 0.295
1.757PhePhe: 1.757 ± 0.424
2.844PheGly: 2.844 ± 0.474
0.586PheHis: 0.586 ± 0.232
3.764PheIle: 3.764 ± 0.675
2.175PheLys: 2.175 ± 0.404
2.008PheLeu: 2.008 ± 0.406
0.837PheMet: 0.837 ± 0.27
2.091PheAsn: 2.091 ± 0.317
1.338PhePro: 1.338 ± 0.345
1.924PheGln: 1.924 ± 0.483
1.422PheArg: 1.422 ± 0.277
3.179PheSer: 3.179 ± 0.578
2.091PheThr: 2.091 ± 0.458
1.506PheVal: 1.506 ± 0.328
0.669PheTrp: 0.669 ± 0.217
1.757PheTyr: 1.757 ± 0.432
0.0PheXaa: 0.0 ± 0.0
Gly
5.521GlyAla: 5.521 ± 0.677
0.418GlyCys: 0.418 ± 0.161
3.681GlyAsp: 3.681 ± 0.535
5.27GlyGlu: 5.27 ± 0.599
2.761GlyPhe: 2.761 ± 0.475
5.019GlyGly: 5.019 ± 0.91
1.673GlyHis: 1.673 ± 0.488
5.438GlyIle: 5.438 ± 0.696
5.354GlyLys: 5.354 ± 0.74
4.266GlyLeu: 4.266 ± 0.707
1.255GlyMet: 1.255 ± 0.279
3.263GlyAsn: 3.263 ± 0.554
1.171GlyPro: 1.171 ± 0.355
3.681GlyGln: 3.681 ± 0.688
4.517GlyArg: 4.517 ± 0.61
4.183GlySer: 4.183 ± 0.781
3.597GlyThr: 3.597 ± 0.604
5.605GlyVal: 5.605 ± 0.558
2.091GlyTrp: 2.091 ± 0.393
2.677GlyTyr: 2.677 ± 0.537
0.0GlyXaa: 0.0 ± 0.0
His
1.088HisAla: 1.088 ± 0.31
0.502HisCys: 0.502 ± 0.258
0.92HisAsp: 0.92 ± 0.3
1.422HisGlu: 1.422 ± 0.32
0.586HisPhe: 0.586 ± 0.216
1.422HisGly: 1.422 ± 0.424
0.669HisHis: 0.669 ± 0.223
0.669HisIle: 0.669 ± 0.241
0.586HisLys: 0.586 ± 0.198
2.259HisLeu: 2.259 ± 0.439
0.669HisMet: 0.669 ± 0.261
0.669HisAsn: 0.669 ± 0.218
0.92HisPro: 0.92 ± 0.271
0.669HisGln: 0.669 ± 0.261
1.673HisArg: 1.673 ± 0.368
0.92HisSer: 0.92 ± 0.232
0.418HisThr: 0.418 ± 0.179
0.837HisVal: 0.837 ± 0.274
0.167HisTrp: 0.167 ± 0.112
0.753HisTyr: 0.753 ± 0.258
0.0HisXaa: 0.0 ± 0.0
Ile
6.19IleAla: 6.19 ± 0.637
0.92IleCys: 0.92 ± 0.28
4.266IleAsp: 4.266 ± 0.605
5.103IleGlu: 5.103 ± 0.651
2.259IlePhe: 2.259 ± 0.65
4.35IleGly: 4.35 ± 0.627
0.753IleHis: 0.753 ± 0.238
4.015IleIle: 4.015 ± 0.863
4.35IleLys: 4.35 ± 0.561
3.263IleLeu: 3.263 ± 0.461
0.669IleMet: 0.669 ± 0.212
3.43IleAsn: 3.43 ± 0.443
2.761IlePro: 2.761 ± 0.484
2.091IleGln: 2.091 ± 0.384
3.764IleArg: 3.764 ± 0.557
4.685IleSer: 4.685 ± 0.565
4.35IleThr: 4.35 ± 0.722
2.761IleVal: 2.761 ± 0.49
0.502IleTrp: 0.502 ± 0.218
2.091IleTyr: 2.091 ± 0.43
0.0IleXaa: 0.0 ± 0.0
Lys
5.019LysAla: 5.019 ± 0.676
0.669LysCys: 0.669 ± 0.205
3.012LysAsp: 3.012 ± 0.461
4.266LysGlu: 4.266 ± 0.642
1.84LysPhe: 1.84 ± 0.39
4.768LysGly: 4.768 ± 0.593
0.92LysHis: 0.92 ± 0.306
3.43LysIle: 3.43 ± 0.471
3.597LysLys: 3.597 ± 0.631
4.434LysLeu: 4.434 ± 0.629
1.84LysMet: 1.84 ± 0.373
1.84LysAsn: 1.84 ± 0.367
3.179LysPro: 3.179 ± 0.406
3.179LysGln: 3.179 ± 0.562
5.27LysArg: 5.27 ± 0.596
4.35LysSer: 4.35 ± 0.653
3.263LysThr: 3.263 ± 0.53
3.932LysVal: 3.932 ± 0.797
1.004LysTrp: 1.004 ± 0.274
2.091LysTyr: 2.091 ± 0.455
0.0LysXaa: 0.0 ± 0.0
Leu
6.943LeuAla: 6.943 ± 0.748
0.92LeuCys: 0.92 ± 0.26
4.015LeuAsp: 4.015 ± 0.543
4.852LeuGlu: 4.852 ± 0.586
2.426LeuPhe: 2.426 ± 0.546
4.685LeuGly: 4.685 ± 0.639
1.088LeuHis: 1.088 ± 0.325
4.936LeuIle: 4.936 ± 0.855
5.438LeuLys: 5.438 ± 0.764
5.019LeuLeu: 5.019 ± 0.702
1.757LeuMet: 1.757 ± 0.433
4.434LeuAsn: 4.434 ± 0.626
3.346LeuPro: 3.346 ± 0.504
2.677LeuGln: 2.677 ± 0.524
4.936LeuArg: 4.936 ± 0.701
5.354LeuSer: 5.354 ± 0.89
5.103LeuThr: 5.103 ± 0.622
4.015LeuVal: 4.015 ± 0.464
1.255LeuTrp: 1.255 ± 0.326
2.342LeuTyr: 2.342 ± 0.455
0.0LeuXaa: 0.0 ± 0.0
Met
3.095MetAla: 3.095 ± 0.593
0.084MetCys: 0.084 ± 0.093
1.171MetAsp: 1.171 ± 0.323
1.004MetGlu: 1.004 ± 0.271
0.251MetPhe: 0.251 ± 0.151
2.008MetGly: 2.008 ± 0.441
0.418MetHis: 0.418 ± 0.18
0.837MetIle: 0.837 ± 0.286
2.008MetLys: 2.008 ± 0.367
1.589MetLeu: 1.589 ± 0.352
0.669MetMet: 0.669 ± 0.227
1.004MetAsn: 1.004 ± 0.304
1.422MetPro: 1.422 ± 0.309
1.84MetGln: 1.84 ± 0.406
1.673MetArg: 1.673 ± 0.445
1.757MetSer: 1.757 ± 0.456
2.008MetThr: 2.008 ± 0.402
1.338MetVal: 1.338 ± 0.326
0.167MetTrp: 0.167 ± 0.121
1.004MetTyr: 1.004 ± 0.282
0.0MetXaa: 0.0 ± 0.0
Asn
5.27AsnAla: 5.27 ± 0.846
0.502AsnCys: 0.502 ± 0.22
2.677AsnAsp: 2.677 ± 0.464
2.593AsnGlu: 2.593 ± 0.424
0.669AsnPhe: 0.669 ± 0.251
3.597AsnGly: 3.597 ± 0.688
1.004AsnHis: 1.004 ± 0.329
2.593AsnIle: 2.593 ± 0.556
4.015AsnLys: 4.015 ± 0.593
3.346AsnLeu: 3.346 ± 0.492
1.84AsnMet: 1.84 ± 0.315
2.259AsnAsn: 2.259 ± 0.515
2.51AsnPro: 2.51 ± 0.448
3.263AsnGln: 3.263 ± 0.622
2.259AsnArg: 2.259 ± 0.549
2.593AsnSer: 2.593 ± 0.534
2.51AsnThr: 2.51 ± 0.392
2.51AsnVal: 2.51 ± 0.717
0.418AsnTrp: 0.418 ± 0.17
2.175AsnTyr: 2.175 ± 0.446
0.0AsnXaa: 0.0 ± 0.0
Pro
3.095ProAla: 3.095 ± 0.475
0.251ProCys: 0.251 ± 0.154
3.263ProAsp: 3.263 ± 0.557
4.183ProGlu: 4.183 ± 0.619
1.506ProPhe: 1.506 ± 0.31
2.259ProGly: 2.259 ± 0.38
0.418ProHis: 0.418 ± 0.222
2.008ProIle: 2.008 ± 0.478
2.593ProLys: 2.593 ± 0.386
2.175ProLeu: 2.175 ± 0.358
0.502ProMet: 0.502 ± 0.182
1.422ProAsn: 1.422 ± 0.282
1.673ProPro: 1.673 ± 0.427
1.338ProGln: 1.338 ± 0.237
1.673ProArg: 1.673 ± 0.38
2.426ProSer: 2.426 ± 0.503
1.84ProThr: 1.84 ± 0.347
3.095ProVal: 3.095 ± 0.458
0.502ProTrp: 0.502 ± 0.195
1.338ProTyr: 1.338 ± 0.378
0.0ProXaa: 0.0 ± 0.0
Gln
4.266GlnAla: 4.266 ± 1.129
0.669GlnCys: 0.669 ± 0.265
2.51GlnAsp: 2.51 ± 0.435
2.51GlnGlu: 2.51 ± 0.477
1.673GlnPhe: 1.673 ± 0.421
3.263GlnGly: 3.263 ± 0.574
1.171GlnHis: 1.171 ± 0.315
2.928GlnIle: 2.928 ± 0.362
2.259GlnLys: 2.259 ± 0.392
4.015GlnLeu: 4.015 ± 0.581
1.589GlnMet: 1.589 ± 0.35
2.51GlnAsn: 2.51 ± 0.647
1.924GlnPro: 1.924 ± 0.396
3.681GlnGln: 3.681 ± 0.855
2.928GlnArg: 2.928 ± 0.462
3.681GlnSer: 3.681 ± 0.588
1.088GlnThr: 1.088 ± 0.305
2.259GlnVal: 2.259 ± 0.451
1.004GlnTrp: 1.004 ± 0.262
2.426GlnTyr: 2.426 ± 0.474
0.0GlnXaa: 0.0 ± 0.0
Arg
5.438ArgAla: 5.438 ± 0.676
0.669ArgCys: 0.669 ± 0.247
4.099ArgAsp: 4.099 ± 0.546
5.019ArgGlu: 5.019 ± 0.611
2.008ArgPhe: 2.008 ± 0.435
3.346ArgGly: 3.346 ± 0.404
1.506ArgHis: 1.506 ± 0.393
3.764ArgIle: 3.764 ± 0.53
3.848ArgLys: 3.848 ± 0.758
5.438ArgLeu: 5.438 ± 0.681
1.255ArgMet: 1.255 ± 0.344
2.426ArgAsn: 2.426 ± 0.326
2.175ArgPro: 2.175 ± 0.314
3.764ArgGln: 3.764 ± 0.648
3.43ArgArg: 3.43 ± 0.717
3.932ArgSer: 3.932 ± 0.628
2.259ArgThr: 2.259 ± 0.347
2.844ArgVal: 2.844 ± 0.482
0.669ArgTrp: 0.669 ± 0.229
2.342ArgTyr: 2.342 ± 0.391
0.0ArgXaa: 0.0 ± 0.0
Ser
5.27SerAla: 5.27 ± 0.826
0.335SerCys: 0.335 ± 0.155
3.681SerAsp: 3.681 ± 0.625
3.597SerGlu: 3.597 ± 0.585
2.844SerPhe: 2.844 ± 0.684
4.852SerGly: 4.852 ± 0.593
1.506SerHis: 1.506 ± 0.379
3.513SerIle: 3.513 ± 0.578
3.681SerLys: 3.681 ± 0.566
5.019SerLeu: 5.019 ± 0.707
2.175SerMet: 2.175 ± 0.42
3.179SerAsn: 3.179 ± 0.644
2.342SerPro: 2.342 ± 0.469
2.677SerGln: 2.677 ± 0.47
3.764SerArg: 3.764 ± 0.59
4.099SerSer: 4.099 ± 0.645
3.263SerThr: 3.263 ± 0.458
3.513SerVal: 3.513 ± 0.61
0.669SerTrp: 0.669 ± 0.26
1.924SerTyr: 1.924 ± 0.41
0.0SerXaa: 0.0 ± 0.0
Thr
4.768ThrAla: 4.768 ± 0.677
1.088ThrCys: 1.088 ± 0.312
3.346ThrAsp: 3.346 ± 0.508
2.593ThrGlu: 2.593 ± 0.573
1.673ThrPhe: 1.673 ± 0.48
6.107ThrGly: 6.107 ± 0.8
0.586ThrHis: 0.586 ± 0.175
3.932ThrIle: 3.932 ± 0.538
2.259ThrLys: 2.259 ± 0.466
4.434ThrLeu: 4.434 ± 0.415
0.92ThrMet: 0.92 ± 0.273
2.091ThrAsn: 2.091 ± 0.46
3.263ThrPro: 3.263 ± 0.442
2.426ThrGln: 2.426 ± 0.494
2.51ThrArg: 2.51 ± 0.404
2.51ThrSer: 2.51 ± 0.542
2.761ThrThr: 2.761 ± 0.555
2.426ThrVal: 2.426 ± 0.368
0.586ThrTrp: 0.586 ± 0.215
1.506ThrTyr: 1.506 ± 0.422
0.0ThrXaa: 0.0 ± 0.0
Val
5.438ValAla: 5.438 ± 0.694
0.669ValCys: 0.669 ± 0.214
3.095ValAsp: 3.095 ± 0.471
3.346ValGlu: 3.346 ± 0.577
2.593ValPhe: 2.593 ± 0.427
2.426ValGly: 2.426 ± 0.352
0.92ValHis: 0.92 ± 0.33
2.51ValIle: 2.51 ± 0.416
3.597ValLys: 3.597 ± 0.466
5.688ValLeu: 5.688 ± 0.799
1.338ValMet: 1.338 ± 0.421
3.513ValAsn: 3.513 ± 0.683
1.506ValPro: 1.506 ± 0.336
2.342ValGln: 2.342 ± 0.48
3.346ValArg: 3.346 ± 0.55
4.015ValSer: 4.015 ± 0.655
4.35ValThr: 4.35 ± 0.621
3.43ValVal: 3.43 ± 0.56
1.004ValTrp: 1.004 ± 0.275
1.757ValTyr: 1.757 ± 0.446
0.0ValXaa: 0.0 ± 0.0
Trp
0.92TrpAla: 0.92 ± 0.301
0.167TrpCys: 0.167 ± 0.112
1.004TrpAsp: 1.004 ± 0.295
1.338TrpGlu: 1.338 ± 0.336
0.669TrpPhe: 0.669 ± 0.209
0.837TrpGly: 0.837 ± 0.21
0.418TrpHis: 0.418 ± 0.168
0.669TrpIle: 0.669 ± 0.229
1.255TrpLys: 1.255 ± 0.35
1.84TrpLeu: 1.84 ± 0.469
0.418TrpMet: 0.418 ± 0.182
0.586TrpAsn: 0.586 ± 0.203
0.669TrpPro: 0.669 ± 0.181
0.92TrpGln: 0.92 ± 0.278
1.338TrpArg: 1.338 ± 0.28
0.837TrpSer: 0.837 ± 0.237
0.418TrpThr: 0.418 ± 0.191
0.92TrpVal: 0.92 ± 0.274
0.335TrpTrp: 0.335 ± 0.147
0.502TrpTyr: 0.502 ± 0.235
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.179TyrAla: 3.179 ± 0.523
0.669TyrCys: 0.669 ± 0.253
2.426TyrAsp: 2.426 ± 0.515
2.342TyrGlu: 2.342 ± 0.419
2.008TyrPhe: 2.008 ± 0.373
2.175TyrGly: 2.175 ± 0.54
0.837TyrHis: 0.837 ± 0.24
2.091TyrIle: 2.091 ± 0.479
1.589TyrLys: 1.589 ± 0.355
2.928TyrLeu: 2.928 ± 0.429
0.669TyrMet: 0.669 ± 0.25
1.757TyrAsn: 1.757 ± 0.423
1.088TyrPro: 1.088 ± 0.327
2.426TyrGln: 2.426 ± 0.445
2.677TyrArg: 2.677 ± 0.469
1.757TyrSer: 1.757 ± 0.404
1.673TyrThr: 1.673 ± 0.312
1.924TyrVal: 1.924 ± 0.36
0.335TyrTrp: 0.335 ± 0.179
1.589TyrTyr: 1.589 ± 0.537
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (11955 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski