Pseudomonas virus DMS3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Casadabanvirus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0SMM3|A0SMM3_9CAUD Uncharacterized protein OS=Pseudomonas virus DMS3 OX=389469 GN=DMS3-13 PE=4 SV=1
MM1 pKa = 7.99ADD3 pKa = 3.94TIAFCWASGLIQFGDD18 pKa = 3.87QVPEE22 pKa = 3.95GAIEE26 pKa = 3.89IARR29 pKa = 11.84GDD31 pKa = 3.75DD32 pKa = 3.35QVVRR36 pKa = 11.84EE37 pKa = 4.02NLVTNSRR44 pKa = 11.84HH45 pKa = 6.22AYY47 pKa = 10.38DD48 pKa = 3.73NVSLLVPGVPEE59 pKa = 4.44AANQTEE65 pKa = 4.29GGDD68 pKa = 3.57ALEE71 pKa = 4.31CFIRR75 pKa = 11.84LLAKK79 pKa = 10.05YY80 pKa = 9.97DD81 pKa = 3.48SAAFQVAYY89 pKa = 10.95VEE91 pKa = 4.8DD92 pKa = 3.9VV93 pKa = 2.95

Molecular weight: 10.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0SMN2|A0SMN2_9CAUD Uncharacterized protein OS=Pseudomonas virus DMS3 OX=389469 GN=DMS3-22 PE=4 SV=1
MM1 pKa = 7.71ALQPRR6 pKa = 11.84GIRR9 pKa = 11.84NNNPGNIVWSARR21 pKa = 11.84NNWQGQLPHH30 pKa = 6.42NPKK33 pKa = 9.79VEE35 pKa = 3.88PRR37 pKa = 11.84FARR40 pKa = 11.84FDD42 pKa = 3.52TAHH45 pKa = 6.2NGIRR49 pKa = 11.84ALAKK53 pKa = 10.57LLLNYY58 pKa = 10.24RR59 pKa = 11.84KK60 pKa = 9.47VHH62 pKa = 5.43GLRR65 pKa = 11.84TVEE68 pKa = 3.93SLIARR73 pKa = 11.84WAPSNEE79 pKa = 3.72NDD81 pKa = 2.89TRR83 pKa = 11.84AYY85 pKa = 8.05ATAVARR91 pKa = 11.84AMGVPPQAGLHH102 pKa = 5.82MDD104 pKa = 3.53QDD106 pKa = 4.01TLAALVTAIIRR117 pKa = 11.84HH118 pKa = 5.57EE119 pKa = 4.47NGQQPYY125 pKa = 9.68SAEE128 pKa = 3.99QIAQAVRR135 pKa = 11.84EE136 pKa = 4.2VLL138 pKa = 3.49

Molecular weight: 15.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11238

38

1160

216.1

23.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.867 ± 0.796

0.819 ± 0.123

5.864 ± 0.235

6.113 ± 0.279

2.767 ± 0.227

7.733 ± 0.309

1.655 ± 0.178

4.342 ± 0.178

3.221 ± 0.274

9.931 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.242 ± 0.156

2.865 ± 0.227

4.93 ± 0.346

4.965 ± 0.264

7.59 ± 0.34

5.811 ± 0.282

5.597 ± 0.288

6.389 ± 0.284

1.664 ± 0.154

2.634 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski