Amino acid dipepetide frequency for Virgibacillus pantothenticus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.719AlaAla: 5.719 ± 0.104	0.667AlaCys: 0.667 ± 0.025	3.521AlaAsp: 3.521 ± 0.057	4.47AlaGlu: 4.47 ± 0.071	3.563AlaPhe: 3.563 ± 0.053	5.049AlaGly: 5.049 ± 0.08	1.343AlaHis: 1.343 ± 0.034	6.471AlaIle: 6.471 ± 0.077	5.034AlaLys: 5.034 ± 0.071	7.207AlaLeu: 7.207 ± 0.087	2.13AlaMet: 2.13 ± 0.047	3.176AlaAsn: 3.176 ± 0.051	1.974AlaPro: 1.974 ± 0.045	2.259AlaGln: 2.259 ± 0.049	2.486AlaArg: 2.486 ± 0.048	4.149AlaSer: 4.149 ± 0.071	3.925AlaThr: 3.925 ± 0.059	5.337AlaVal: 5.337 ± 0.077	0.672AlaTrp: 0.672 ± 0.026	2.681AlaTyr: 2.681 ± 0.049	0.003AlaXaa: 0.003 ± 0.002
Cys	0.424CysAla: 0.424 ± 0.017	0.093CysCys: 0.093 ± 0.011	0.381CysAsp: 0.381 ± 0.018	0.423CysGlu: 0.423 ± 0.021	0.308CysPhe: 0.308 ± 0.016	0.586CysGly: 0.586 ± 0.024	0.205CysHis: 0.205 ± 0.012	0.55CysIle: 0.55 ± 0.021	0.44CysLys: 0.44 ± 0.022	0.654CysLeu: 0.654 ± 0.026	0.188CysMet: 0.188 ± 0.014	0.326CysAsn: 0.326 ± 0.017	0.317CysPro: 0.317 ± 0.019	0.254CysGln: 0.254 ± 0.015	0.251CysArg: 0.251 ± 0.015	0.476CysSer: 0.476 ± 0.022	0.399CysThr: 0.399 ± 0.017	0.384CysVal: 0.384 ± 0.021	0.064CysTrp: 0.064 ± 0.008	0.258CysTyr: 0.258 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.119AspAla: 3.119 ± 0.06	0.356AspCys: 0.356 ± 0.018	2.442AspAsp: 2.442 ± 0.05	4.147AspGlu: 4.147 ± 0.074	2.356AspPhe: 2.356 ± 0.044	3.25AspGly: 3.25 ± 0.065	1.164AspHis: 1.164 ± 0.034	4.216AspIle: 4.216 ± 0.063	3.571AspLys: 3.571 ± 0.057	4.727AspLeu: 4.727 ± 0.062	1.491AspMet: 1.491 ± 0.038	2.072AspAsn: 2.072 ± 0.041	1.967AspPro: 1.967 ± 0.044	2.114AspGln: 2.114 ± 0.049	2.095AspArg: 2.095 ± 0.043	2.416AspSer: 2.416 ± 0.05	2.49AspThr: 2.49 ± 0.048	3.851AspVal: 3.851 ± 0.065	0.598AspTrp: 0.598 ± 0.025	2.311AspTyr: 2.311 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	5.815GluAla: 5.815 ± 0.073	0.327GluCys: 0.327 ± 0.018	3.521GluAsp: 3.521 ± 0.058	6.637GluGlu: 6.637 ± 0.101	2.351GluPhe: 2.351 ± 0.055	3.916GluGly: 3.916 ± 0.067	1.511GluHis: 1.511 ± 0.034	5.41GluIle: 5.41 ± 0.066	6.448GluLys: 6.448 ± 0.074	6.888GluLeu: 6.888 ± 0.09	2.214GluMet: 2.214 ± 0.047	3.651GluAsn: 3.651 ± 0.071	2.037GluPro: 2.037 ± 0.04	4.055GluGln: 4.055 ± 0.083	3.264GluArg: 3.264 ± 0.05	3.1GluSer: 3.1 ± 0.051	3.828GluThr: 3.828 ± 0.062	4.884GluVal: 4.884 ± 0.072	0.762GluTrp: 0.762 ± 0.027	2.039GluTyr: 2.039 ± 0.05	0.001GluXaa: 0.001 ± 0.001
Phe	3.219PheAla: 3.219 ± 0.05	0.355PheCys: 0.355 ± 0.018	2.188PheAsp: 2.188 ± 0.045	2.439PheGlu: 2.439 ± 0.049	2.339PhePhe: 2.339 ± 0.054	3.114PheGly: 3.114 ± 0.058	1.028PheHis: 1.028 ± 0.031	4.348PheIle: 4.348 ± 0.079	2.328PheLys: 2.328 ± 0.044	4.594PheLeu: 4.594 ± 0.086	1.264PheMet: 1.264 ± 0.038	1.851PheAsn: 1.851 ± 0.042	1.624PhePro: 1.624 ± 0.039	1.839PheGln: 1.839 ± 0.043	1.485PheArg: 1.485 ± 0.033	3.214PheSer: 3.214 ± 0.055	2.772PheThr: 2.772 ± 0.048	3.048PheVal: 3.048 ± 0.052	0.458PheTrp: 0.458 ± 0.021	1.803PheTyr: 1.803 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	4.759GlyAla: 4.759 ± 0.091	0.544GlyCys: 0.544 ± 0.022	3.116GlyAsp: 3.116 ± 0.055	4.481GlyGlu: 4.481 ± 0.076	3.301GlyPhe: 3.301 ± 0.062	4.588GlyGly: 4.588 ± 0.088	1.248GlyHis: 1.248 ± 0.035	5.978GlyIle: 5.978 ± 0.077	5.124GlyLys: 5.124 ± 0.065	6.322GlyLeu: 6.322 ± 0.091	2.044GlyMet: 2.044 ± 0.048	2.805GlyAsn: 2.805 ± 0.053	1.607GlyPro: 1.607 ± 0.039	1.859GlyGln: 1.859 ± 0.041	2.343GlyArg: 2.343 ± 0.048	3.662GlySer: 3.662 ± 0.055	3.875GlyThr: 3.875 ± 0.055	4.978GlyVal: 4.978 ± 0.077	0.763GlyTrp: 0.763 ± 0.028	2.804GlyTyr: 2.804 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.536HisAla: 1.536 ± 0.038	0.207HisCys: 0.207 ± 0.015	1.059HisAsp: 1.059 ± 0.033	1.427HisGlu: 1.427 ± 0.032	1.082HisPhe: 1.082 ± 0.032	1.488HisGly: 1.488 ± 0.042	0.73HisHis: 0.73 ± 0.026	1.698HisIle: 1.698 ± 0.04	1.142HisLys: 1.142 ± 0.032	2.223HisLeu: 2.223 ± 0.043	0.596HisMet: 0.596 ± 0.021	0.805HisAsn: 0.805 ± 0.029	1.123HisPro: 1.123 ± 0.034	1.02HisGln: 1.02 ± 0.033	0.915HisArg: 0.915 ± 0.029	1.165HisSer: 1.165 ± 0.035	1.089HisThr: 1.089 ± 0.033	1.552HisVal: 1.552 ± 0.039	0.218HisTrp: 0.218 ± 0.014	0.925HisTyr: 0.925 ± 0.028	0.001HisXaa: 0.001 ± 0.001
Ile	6.801IleAla: 6.801 ± 0.092	0.665IleCys: 0.665 ± 0.026	4.374IleAsp: 4.374 ± 0.066	5.404IleGlu: 5.404 ± 0.068	3.566IlePhe: 3.566 ± 0.069	6.312IleGly: 6.312 ± 0.088	1.919IleHis: 1.919 ± 0.046	6.741IleIle: 6.741 ± 0.108	4.686IleLys: 4.686 ± 0.069	7.214IleLeu: 7.214 ± 0.111	1.985IleMet: 1.985 ± 0.047	3.41IleAsn: 3.41 ± 0.059	3.61IlePro: 3.61 ± 0.061	3.266IleGln: 3.266 ± 0.061	3.198IleArg: 3.198 ± 0.056	5.11IleSer: 5.11 ± 0.071	4.809IleThr: 4.809 ± 0.074	5.67IleVal: 5.67 ± 0.079	0.719IleTrp: 0.719 ± 0.026	2.645IleTyr: 2.645 ± 0.047	0.001IleXaa: 0.001 ± 0.001
Lys	4.751LysAla: 4.751 ± 0.066	0.33LysCys: 0.33 ± 0.019	3.843LysAsp: 3.843 ± 0.057	6.751LysGlu: 6.751 ± 0.088	1.746LysPhe: 1.746 ± 0.039	4.176LysGly: 4.176 ± 0.068	1.572LysHis: 1.572 ± 0.032	4.598LysIle: 4.598 ± 0.067	5.799LysLys: 5.799 ± 0.095	5.978LysLeu: 5.978 ± 0.078	2.144LysMet: 2.144 ± 0.034	3.372LysAsn: 3.372 ± 0.057	2.235LysPro: 2.235 ± 0.043	4.43LysGln: 4.43 ± 0.077	3.342LysArg: 3.342 ± 0.06	3.341LysSer: 3.341 ± 0.058	3.666LysThr: 3.666 ± 0.053	4.246LysVal: 4.246 ± 0.066	0.75LysTrp: 0.75 ± 0.029	2.238LysTyr: 2.238 ± 0.041	0.001LysXaa: 0.001 ± 0.001
Leu	7.387LeuAla: 7.387 ± 0.086	0.606LeuCys: 0.606 ± 0.022	4.711LeuAsp: 4.711 ± 0.065	6.448LeuGlu: 6.448 ± 0.076	4.931LeuPhe: 4.931 ± 0.088	6.293LeuGly: 6.293 ± 0.086	2.165LeuHis: 2.165 ± 0.055	7.481LeuIle: 7.481 ± 0.112	6.216LeuLys: 6.216 ± 0.08	9.998LeuLeu: 9.998 ± 0.122	2.496LeuMet: 2.496 ± 0.049	3.979LeuAsn: 3.979 ± 0.07	3.895LeuPro: 3.895 ± 0.066	4.363LeuGln: 4.363 ± 0.073	3.553LeuArg: 3.553 ± 0.06	6.225LeuSer: 6.225 ± 0.085	5.735LeuThr: 5.735 ± 0.075	5.95LeuVal: 5.95 ± 0.08	0.8LeuTrp: 0.8 ± 0.026	3.149LeuTyr: 3.149 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	1.974MetAla: 1.974 ± 0.042	0.135MetCys: 0.135 ± 0.011	1.68MetAsp: 1.68 ± 0.038	2.259MetGlu: 2.259 ± 0.046	1.128MetPhe: 1.128 ± 0.033	1.756MetGly: 1.756 ± 0.04	0.499MetHis: 0.499 ± 0.021	2.173MetIle: 2.173 ± 0.043	2.457MetLys: 2.457 ± 0.041	2.679MetLeu: 2.679 ± 0.057	0.829MetMet: 0.829 ± 0.028	1.583MetAsn: 1.583 ± 0.038	0.947MetPro: 0.947 ± 0.03	1.196MetGln: 1.196 ± 0.035	1.115MetArg: 1.115 ± 0.033	1.561MetSer: 1.561 ± 0.036	1.576MetThr: 1.576 ± 0.04	1.906MetVal: 1.906 ± 0.041	0.181MetTrp: 0.181 ± 0.013	0.835MetTyr: 0.835 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.77AsnAla: 2.77 ± 0.051	0.326AsnCys: 0.326 ± 0.019	2.329AsnAsp: 2.329 ± 0.052	3.453AsnGlu: 3.453 ± 0.057	1.649AsnPhe: 1.649 ± 0.046	3.173AsnGly: 3.173 ± 0.065	1.145AsnHis: 1.145 ± 0.032	3.496AsnIle: 3.496 ± 0.056	3.359AsnLys: 3.359 ± 0.065	3.672AsnLeu: 3.672 ± 0.058	1.302AsnMet: 1.302 ± 0.038	2.165AsnAsn: 2.165 ± 0.062	1.999AsnPro: 1.999 ± 0.045	2.198AsnGln: 2.198 ± 0.045	1.948AsnArg: 1.948 ± 0.045	2.19AsnSer: 2.19 ± 0.047	2.176AsnThr: 2.176 ± 0.045	2.866AsnVal: 2.866 ± 0.055	0.555AsnTrp: 0.555 ± 0.021	1.841AsnTyr: 1.841 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	2.281ProAla: 2.281 ± 0.054	0.222ProCys: 0.222 ± 0.014	1.963ProAsp: 1.963 ± 0.04	2.801ProGlu: 2.801 ± 0.053	1.964ProPhe: 1.964 ± 0.045	2.161ProGly: 2.161 ± 0.057	0.84ProHis: 0.84 ± 0.026	3.14ProIle: 3.14 ± 0.052	2.203ProLys: 2.203 ± 0.05	3.348ProLeu: 3.348 ± 0.057	0.88ProMet: 0.88 ± 0.028	1.754ProAsn: 1.754 ± 0.041	0.929ProPro: 0.929 ± 0.032	1.051ProGln: 1.051 ± 0.031	1.013ProArg: 1.013 ± 0.033	2.117ProSer: 2.117 ± 0.043	2.026ProThr: 2.026 ± 0.044	2.745ProVal: 2.745 ± 0.049	0.347ProTrp: 0.347 ± 0.021	1.407ProTyr: 1.407 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	3.55GlnAla: 3.55 ± 0.062	0.172GlnCys: 0.172 ± 0.012	1.871GlnAsp: 1.871 ± 0.043	3.293GlnGlu: 3.293 ± 0.062	1.695GlnPhe: 1.695 ± 0.038	2.407GlnGly: 2.407 ± 0.049	0.976GlnHis: 0.976 ± 0.033	3.045GlnIle: 3.045 ± 0.06	2.786GlnLys: 2.786 ± 0.06	4.848GlnLeu: 4.848 ± 0.085	1.289GlnMet: 1.289 ± 0.033	1.662GlnAsn: 1.662 ± 0.044	1.357GlnPro: 1.357 ± 0.036	2.528GlnGln: 2.528 ± 0.065	1.699GlnArg: 1.699 ± 0.041	2.22GlnSer: 2.22 ± 0.049	2.42GlnThr: 2.42 ± 0.046	2.665GlnVal: 2.665 ± 0.051	0.444GlnTrp: 0.444 ± 0.019	1.311GlnTyr: 1.311 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.323ArgAla: 2.323 ± 0.048	0.288ArgCys: 0.288 ± 0.017	1.9ArgAsp: 1.9 ± 0.044	2.903ArgGlu: 2.903 ± 0.048	1.883ArgPhe: 1.883 ± 0.042	2.141ArgGly: 2.141 ± 0.051	0.792ArgHis: 0.792 ± 0.024	3.114ArgIle: 3.114 ± 0.054	3.248ArgLys: 3.248 ± 0.057	3.883ArgLeu: 3.883 ± 0.062	1.234ArgMet: 1.234 ± 0.04	1.918ArgAsn: 1.918 ± 0.043	1.141ArgPro: 1.141 ± 0.034	1.621ArgGln: 1.621 ± 0.041	1.686ArgArg: 1.686 ± 0.046	2.146ArgSer: 2.146 ± 0.042	2.071ArgThr: 2.071 ± 0.044	2.407ArgVal: 2.407 ± 0.042	0.391ArgTrp: 0.391 ± 0.017	1.56ArgTyr: 1.56 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	3.4SerAla: 3.4 ± 0.054	0.386SerCys: 0.386 ± 0.019	2.751SerAsp: 2.751 ± 0.051	3.652SerGlu: 3.652 ± 0.068	3.201SerPhe: 3.201 ± 0.056	4.088SerGly: 4.088 ± 0.064	1.163SerHis: 1.163 ± 0.029	5.189SerIle: 5.189 ± 0.071	3.689SerLys: 3.689 ± 0.062	5.689SerLeu: 5.689 ± 0.068	1.73SerMet: 1.73 ± 0.041	2.434SerAsn: 2.434 ± 0.053	1.928SerPro: 1.928 ± 0.043	1.976SerGln: 1.976 ± 0.042	2.053SerArg: 2.053 ± 0.044	3.558SerSer: 3.558 ± 0.061	3.051SerThr: 3.051 ± 0.049	3.848SerVal: 3.848 ± 0.062	0.64SerTrp: 0.64 ± 0.025	2.277SerTyr: 2.277 ± 0.046	0.003SerXaa: 0.003 ± 0.002
Thr	4.088ThrAla: 4.088 ± 0.066	0.372ThrCys: 0.372 ± 0.015	2.839ThrAsp: 2.839 ± 0.053	3.73ThrGlu: 3.73 ± 0.06	2.691ThrPhe: 2.691 ± 0.052	4.093ThrGly: 4.093 ± 0.056	1.061ThrHis: 1.061 ± 0.034	5.065ThrIle: 5.065 ± 0.069	3.661ThrLys: 3.661 ± 0.062	5.072ThrLeu: 5.072 ± 0.076	1.449ThrMet: 1.449 ± 0.037	2.672ThrAsn: 2.672 ± 0.047	2.271ThrPro: 2.271 ± 0.041	1.506ThrGln: 1.506 ± 0.035	1.74ThrArg: 1.74 ± 0.039	3.35ThrSer: 3.35 ± 0.059	3.174ThrThr: 3.174 ± 0.053	4.1ThrVal: 4.1 ± 0.069	0.569ThrTrp: 0.569 ± 0.024	2.18ThrTyr: 2.18 ± 0.046	0.001ThrXaa: 0.001 ± 0.001
Val	5.071ValAla: 5.071 ± 0.08	0.573ValCys: 0.573 ± 0.024	3.742ValAsp: 3.742 ± 0.064	4.704ValGlu: 4.704 ± 0.076	3.22ValPhe: 3.22 ± 0.057	4.459ValGly: 4.459 ± 0.066	1.466ValHis: 1.466 ± 0.035	5.904ValIle: 5.904 ± 0.082	4.308ValLys: 4.308 ± 0.059	6.568ValLeu: 6.568 ± 0.083	1.886ValMet: 1.886 ± 0.039	3.001ValAsn: 3.001 ± 0.061	2.544ValPro: 2.544 ± 0.049	2.437ValGln: 2.437 ± 0.048	2.407ValArg: 2.407 ± 0.05	4.162ValSer: 4.162 ± 0.055	4.11ValThr: 4.11 ± 0.059	4.73ValVal: 4.73 ± 0.086	0.603ValTrp: 0.603 ± 0.024	2.332ValTyr: 2.332 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.564TrpAla: 0.564 ± 0.022	0.066TrpCys: 0.066 ± 0.007	0.527TrpAsp: 0.527 ± 0.02	0.687TrpGlu: 0.687 ± 0.027	0.53TrpPhe: 0.53 ± 0.024	0.627TrpGly: 0.627 ± 0.026	0.214TrpHis: 0.214 ± 0.015	0.843TrpIle: 0.843 ± 0.029	0.764TrpLys: 0.764 ± 0.028	1.167TrpLeu: 1.167 ± 0.033	0.35TrpMet: 0.35 ± 0.019	0.501TrpAsn: 0.501 ± 0.022	0.245TrpPro: 0.245 ± 0.017	0.415TrpGln: 0.415 ± 0.018	0.401TrpArg: 0.401 ± 0.02	0.566TrpSer: 0.566 ± 0.023	0.47TrpThr: 0.47 ± 0.019	0.636TrpVal: 0.636 ± 0.023	0.156TrpTrp: 0.156 ± 0.011	0.383TrpTyr: 0.383 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.284TyrAla: 2.284 ± 0.042	0.291TyrCys: 0.291 ± 0.016	1.996TyrAsp: 1.996 ± 0.046	2.553TyrGlu: 2.553 ± 0.049	1.804TyrPhe: 1.804 ± 0.042	2.519TyrGly: 2.519 ± 0.047	0.963TyrHis: 0.963 ± 0.034	2.643TyrIle: 2.643 ± 0.051	2.044TyrLys: 2.044 ± 0.044	3.651TyrLeu: 3.651 ± 0.068	0.967TyrMet: 0.967 ± 0.034	1.472TyrAsn: 1.472 ± 0.043	1.44TyrPro: 1.44 ± 0.032	1.853TyrGln: 1.853 ± 0.039	1.657TyrArg: 1.657 ± 0.046	1.977TyrSer: 1.977 ± 0.037	2.021TyrThr: 2.021 ± 0.043	2.423TyrVal: 2.423 ± 0.045	0.423TyrTrp: 0.423 ± 0.022	1.586TyrTyr: 1.586 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3923 proteins (1123997 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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