Amino acid dipepetide frequency for Nosema apis BRL 01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.012AlaAla: 1.012 ± 0.055	0.542AlaCys: 0.542 ± 0.025	1.096AlaAsp: 1.096 ± 0.037	1.365AlaGlu: 1.365 ± 0.067	1.285AlaPhe: 1.285 ± 0.042	1.096AlaGly: 1.096 ± 0.074	0.454AlaHis: 0.454 ± 0.028	2.168AlaIle: 2.168 ± 0.065	2.095AlaLys: 2.095 ± 0.062	2.358AlaLeu: 2.358 ± 0.06	0.542AlaMet: 0.542 ± 0.031	1.567AlaAsn: 1.567 ± 0.053	0.588AlaPro: 0.588 ± 0.037	0.694AlaGln: 0.694 ± 0.033	0.925AlaArg: 0.925 ± 0.044	1.605AlaSer: 1.605 ± 0.055	1.118AlaThr: 1.118 ± 0.043	1.259AlaVal: 1.259 ± 0.047	0.144AlaTrp: 0.144 ± 0.015	1.202AlaTyr: 1.202 ± 0.044	0.003AlaXaa: 0.003 ± 0.002
Cys	0.446CysAla: 0.446 ± 0.025	0.416CysCys: 0.416 ± 0.026	1.266CysAsp: 1.266 ± 0.05	1.355CysGlu: 1.355 ± 0.044	1.034CysPhe: 1.034 ± 0.045	1.124CysGly: 1.124 ± 0.048	0.197CysHis: 0.197 ± 0.018	2.012CysIle: 2.012 ± 0.058	2.234CysLys: 2.234 ± 0.058	1.847CysLeu: 1.847 ± 0.051	0.432CysMet: 0.432 ± 0.027	1.841CysAsn: 1.841 ± 0.063	0.437CysPro: 0.437 ± 0.027	0.381CysGln: 0.381 ± 0.022	0.666CysArg: 0.666 ± 0.035	1.405CysSer: 1.405 ± 0.051	0.884CysThr: 0.884 ± 0.033	1.181CysVal: 1.181 ± 0.046	0.096CysTrp: 0.096 ± 0.011	0.85CysTyr: 0.85 ± 0.031	0.0CysXaa: 0.0 ± 0.0
Asp	1.354AspAla: 1.354 ± 0.044	1.06AspCys: 1.06 ± 0.042	3.352AspAsp: 3.352 ± 0.082	4.815AspGlu: 4.815 ± 0.09	3.425AspPhe: 3.425 ± 0.067	2.386AspGly: 2.386 ± 0.078	0.798AspHis: 0.798 ± 0.034	6.457AspIle: 6.457 ± 0.106	5.967AspLys: 5.967 ± 0.114	5.897AspLeu: 5.897 ± 0.081	1.171AspMet: 1.171 ± 0.04	5.976AspAsn: 5.976 ± 0.143	1.157AspPro: 1.157 ± 0.043	1.223AspGln: 1.223 ± 0.043	1.711AspArg: 1.711 ± 0.05	3.729AspSer: 3.729 ± 0.094	2.695AspThr: 2.695 ± 0.061	3.124AspVal: 3.124 ± 0.075	0.348AspTrp: 0.348 ± 0.022	2.544AspTyr: 2.544 ± 0.069	0.0AspXaa: 0.0 ± 0.0
Glu	1.798GluAla: 1.798 ± 0.076	1.601GluCys: 1.601 ± 0.064	4.2GluAsp: 4.2 ± 0.1	5.556GluGlu: 5.556 ± 0.143	3.498GluPhe: 3.498 ± 0.072	2.278GluGly: 2.278 ± 0.092	1.242GluHis: 1.242 ± 0.041	8.023GluIle: 8.023 ± 0.119	7.501GluLys: 7.501 ± 0.136	5.747GluLeu: 5.747 ± 0.093	1.606GluMet: 1.606 ± 0.046	8.094GluAsn: 8.094 ± 0.145	1.053GluPro: 1.053 ± 0.042	1.846GluGln: 1.846 ± 0.053	2.071GluArg: 2.071 ± 0.059	4.33GluSer: 4.33 ± 0.107	2.945GluThr: 2.945 ± 0.06	2.929GluVal: 2.929 ± 0.07	0.406GluTrp: 0.406 ± 0.021	3.431GluTyr: 3.431 ± 0.07	0.0GluXaa: 0.0 ± 0.0
Phe	1.052PheAla: 1.052 ± 0.041	1.206PheCys: 1.206 ± 0.042	3.403PheAsp: 3.403 ± 0.079	3.525PheGlu: 3.525 ± 0.074	2.792PhePhe: 2.792 ± 0.072	1.759PheGly: 1.759 ± 0.061	0.721PheHis: 0.721 ± 0.033	5.756PheIle: 5.756 ± 0.104	5.44PheLys: 5.44 ± 0.092	5.308PheLeu: 5.308 ± 0.1	1.137PheMet: 1.137 ± 0.039	5.214PheAsn: 5.214 ± 0.092	1.009PhePro: 1.009 ± 0.033	1.046PheGln: 1.046 ± 0.041	1.395PheArg: 1.395 ± 0.045	3.714PheSer: 3.714 ± 0.076	2.303PheThr: 2.303 ± 0.059	2.688PheVal: 2.688 ± 0.066	0.297PheTrp: 0.297 ± 0.02	3.013PheTyr: 3.013 ± 0.071	0.0PheXaa: 0.0 ± 0.0
Gly	1.136GlyAla: 1.136 ± 0.061	0.838GlyCys: 0.838 ± 0.04	2.26GlyAsp: 2.26 ± 0.093	2.052GlyGlu: 2.052 ± 0.1	1.829GlyPhe: 1.829 ± 0.062	1.887GlyGly: 1.887 ± 0.102	0.748GlyHis: 0.748 ± 0.04	3.597GlyIle: 3.597 ± 0.082	2.991GlyLys: 2.991 ± 0.076	3.1GlyLeu: 3.1 ± 0.109	0.81GlyMet: 0.81 ± 0.036	2.992GlyAsn: 2.992 ± 0.098	0.608GlyPro: 0.608 ± 0.034	0.792GlyGln: 0.792 ± 0.035	1.31GlyArg: 1.31 ± 0.041	2.555GlySer: 2.555 ± 0.087	1.799GlyThr: 1.799 ± 0.061	1.944GlyVal: 1.944 ± 0.06	0.213GlyTrp: 0.213 ± 0.018	1.627GlyTyr: 1.627 ± 0.057	0.003GlyXaa: 0.003 ± 0.002
His	0.39HisAla: 0.39 ± 0.021	0.367HisCys: 0.367 ± 0.025	0.916HisAsp: 0.916 ± 0.04	0.998HisGlu: 0.998 ± 0.032	0.838HisPhe: 0.838 ± 0.033	0.644HisGly: 0.644 ± 0.033	0.422HisHis: 0.422 ± 0.031	1.598HisIle: 1.598 ± 0.047	1.669HisLys: 1.669 ± 0.058	1.755HisLeu: 1.755 ± 0.054	0.292HisMet: 0.292 ± 0.019	1.637HisAsn: 1.637 ± 0.063	0.581HisPro: 0.581 ± 0.035	0.433HisGln: 0.433 ± 0.026	0.605HisArg: 0.605 ± 0.027	1.218HisSer: 1.218 ± 0.051	0.83HisThr: 0.83 ± 0.038	0.817HisVal: 0.817 ± 0.032	0.089HisTrp: 0.089 ± 0.01	0.666HisTyr: 0.666 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	1.96IleAla: 1.96 ± 0.056	2.174IleCys: 2.174 ± 0.059	6.46IleAsp: 6.46 ± 0.107	7.192IleGlu: 7.192 ± 0.103	5.848IlePhe: 5.848 ± 0.104	3.06IleGly: 3.06 ± 0.076	1.805IleHis: 1.805 ± 0.054	9.284IleIle: 9.284 ± 0.132	11.544IleLys: 11.544 ± 0.152	10.305IleLeu: 10.305 ± 0.126	1.767IleMet: 1.767 ± 0.05	11.563IleAsn: 11.563 ± 0.173	2.735IlePro: 2.735 ± 0.06	2.8IleGln: 2.8 ± 0.069	3.007IleArg: 3.007 ± 0.067	7.469IleSer: 7.469 ± 0.105	4.365IleThr: 4.365 ± 0.075	4.394IleVal: 4.394 ± 0.09	0.482IleTrp: 0.482 ± 0.026	5.156IleTyr: 5.156 ± 0.086	0.003IleXaa: 0.003 ± 0.002
Lys	2.212LysAla: 2.212 ± 0.073	2.131LysCys: 2.131 ± 0.071	6.131LysAsp: 6.131 ± 0.114	7.481LysGlu: 7.481 ± 0.132	5.362LysPhe: 5.362 ± 0.105	2.716LysGly: 2.716 ± 0.083	1.923LysHis: 1.923 ± 0.056	12.144LysIle: 12.144 ± 0.153	10.937LysLys: 10.937 ± 0.156	9.267LysLeu: 9.267 ± 0.128	2.52LysMet: 2.52 ± 0.059	11.799LysAsn: 11.799 ± 0.175	1.968LysPro: 1.968 ± 0.049	2.9LysGln: 2.9 ± 0.069	3.349LysArg: 3.349 ± 0.071	6.974LysSer: 6.974 ± 0.13	4.966LysThr: 4.966 ± 0.091	3.873LysVal: 3.873 ± 0.076	0.597LysTrp: 0.597 ± 0.031	5.909LysTyr: 5.909 ± 0.125	0.003LysXaa: 0.003 ± 0.002
Leu	2.172LeuAla: 2.172 ± 0.069	2.023LeuCys: 2.023 ± 0.063	5.175LeuAsp: 5.175 ± 0.091	6.628LeuGlu: 6.628 ± 0.115	4.907LeuPhe: 4.907 ± 0.085	3.033LeuGly: 3.033 ± 0.1	1.594LeuHis: 1.594 ± 0.053	8.861LeuIle: 8.861 ± 0.13	10.747LeuLys: 10.747 ± 0.153	8.286LeuLeu: 8.286 ± 0.134	1.722LeuMet: 1.722 ± 0.046	9.825LeuAsn: 9.825 ± 0.146	2.222LeuPro: 2.222 ± 0.063	2.98LeuGln: 2.98 ± 0.064	2.924LeuArg: 2.924 ± 0.07	6.708LeuSer: 6.708 ± 0.091	4.01LeuThr: 4.01 ± 0.074	4.072LeuVal: 4.072 ± 0.076	0.413LeuTrp: 0.413 ± 0.022	4.527LeuTyr: 4.527 ± 0.091	0.004LeuXaa: 0.004 ± 0.002
Met	0.592MetAla: 0.592 ± 0.031	0.49MetCys: 0.49 ± 0.028	1.199MetAsp: 1.199 ± 0.041	1.433MetGlu: 1.433 ± 0.055	1.167MetPhe: 1.167 ± 0.043	0.729MetGly: 0.729 ± 0.036	0.332MetHis: 0.332 ± 0.019	1.75MetIle: 1.75 ± 0.045	2.078MetLys: 2.078 ± 0.059	1.889MetLeu: 1.889 ± 0.053	0.466MetMet: 0.466 ± 0.033	1.862MetAsn: 1.862 ± 0.049	0.446MetPro: 0.446 ± 0.027	0.522MetGln: 0.522 ± 0.026	0.744MetArg: 0.744 ± 0.033	1.477MetSer: 1.477 ± 0.041	0.993MetThr: 0.993 ± 0.04	0.884MetVal: 0.884 ± 0.037	0.168MetTrp: 0.168 ± 0.016	1.065MetTyr: 1.065 ± 0.041	0.003MetXaa: 0.003 ± 0.002
Asn	1.655AsnAla: 1.655 ± 0.049	1.667AsnCys: 1.667 ± 0.054	5.948AsnAsp: 5.948 ± 0.109	7.901AsnGlu: 7.901 ± 0.152	5.24AsnPhe: 5.24 ± 0.096	3.085AsnGly: 3.085 ± 0.089	1.351AsnHis: 1.351 ± 0.05	12.757AsnIle: 12.757 ± 0.215	11.395AsnLys: 11.395 ± 0.171	10.333AsnLeu: 10.333 ± 0.166	2.03AsnMet: 2.03 ± 0.051	13.245AsnAsn: 13.245 ± 0.346	1.726AsnPro: 1.726 ± 0.058	2.415AsnGln: 2.415 ± 0.063	2.772AsnArg: 2.772 ± 0.082	6.777AsnSer: 6.777 ± 0.131	5.314AsnThr: 5.314 ± 0.161	4.802AsnVal: 4.802 ± 0.1	0.393AsnTrp: 0.393 ± 0.024	4.299AsnTyr: 4.299 ± 0.079	0.003AsnXaa: 0.003 ± 0.002
Pro	0.534ProAla: 0.534 ± 0.035	0.376ProCys: 0.376 ± 0.023	1.175ProAsp: 1.175 ± 0.046	1.453ProGlu: 1.453 ± 0.044	1.303ProPhe: 1.303 ± 0.051	0.754ProGly: 0.754 ± 0.034	0.41ProHis: 0.41 ± 0.023	2.25ProIle: 2.25 ± 0.054	1.94ProLys: 1.94 ± 0.049	1.919ProLeu: 1.919 ± 0.055	0.308ProMet: 0.308 ± 0.019	1.718ProAsn: 1.718 ± 0.058	0.717ProPro: 0.717 ± 0.055	0.69ProGln: 0.69 ± 0.042	0.773ProArg: 0.773 ± 0.037	1.73ProSer: 1.73 ± 0.061	1.239ProThr: 1.239 ± 0.06	1.159ProVal: 1.159 ± 0.045	0.125ProTrp: 0.125 ± 0.014	1.191ProTyr: 1.191 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	0.825GlnAla: 0.825 ± 0.042	0.373GlnCys: 0.373 ± 0.022	1.457GlnAsp: 1.457 ± 0.047	1.862GlnGlu: 1.862 ± 0.053	1.23GlnPhe: 1.23 ± 0.043	0.804GlnGly: 0.804 ± 0.035	0.462GlnHis: 0.462 ± 0.024	2.84GlnIle: 2.84 ± 0.058	2.628GlnLys: 2.628 ± 0.073	2.096GlnLeu: 2.096 ± 0.056	0.54GlnMet: 0.54 ± 0.029	2.801GlnAsn: 2.801 ± 0.067	0.676GlnPro: 0.676 ± 0.056	0.97GlnGln: 0.97 ± 0.054	0.836GlnArg: 0.836 ± 0.033	1.893GlnSer: 1.893 ± 0.061	1.166GlnThr: 1.166 ± 0.047	1.121GlnVal: 1.121 ± 0.038	0.125GlnTrp: 0.125 ± 0.011	1.125GlnTyr: 1.125 ± 0.046	0.001GlnXaa: 0.001 ± 0.001
Arg	0.968ArgAla: 0.968 ± 0.04	0.721ArgCys: 0.721 ± 0.032	1.769ArgAsp: 1.769 ± 0.065	2.075ArgGlu: 2.075 ± 0.064	1.513ArgPhe: 1.513 ± 0.051	1.269ArgGly: 1.269 ± 0.046	0.613ArgHis: 0.613 ± 0.03	3.041ArgIle: 3.041 ± 0.066	3.305ArgLys: 3.305 ± 0.079	2.737ArgLeu: 2.737 ± 0.057	0.793ArgMet: 0.793 ± 0.035	2.801ArgAsn: 2.801 ± 0.083	0.729ArgPro: 0.729 ± 0.034	0.852ArgGln: 0.852 ± 0.035	1.579ArgArg: 1.579 ± 0.057	2.016ArgSer: 2.016 ± 0.056	1.363ArgThr: 1.363 ± 0.044	1.505ArgVal: 1.505 ± 0.052	0.187ArgTrp: 0.187 ± 0.016	1.418ArgTyr: 1.418 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	1.529SerAla: 1.529 ± 0.05	1.148SerCys: 1.148 ± 0.038	4.148SerAsp: 4.148 ± 0.094	4.479SerGlu: 4.479 ± 0.089	3.584SerPhe: 3.584 ± 0.067	2.816SerGly: 2.816 ± 0.105	1.185SerHis: 1.185 ± 0.039	7.06SerIle: 7.06 ± 0.108	7.174SerLys: 7.174 ± 0.116	6.349SerLeu: 6.349 ± 0.107	1.27SerMet: 1.27 ± 0.044	7.353SerAsn: 7.353 ± 0.152	1.49SerPro: 1.49 ± 0.099	1.675SerGln: 1.675 ± 0.055	2.062SerArg: 2.062 ± 0.067	5.973SerSer: 5.973 ± 0.159	3.968SerThr: 3.968 ± 0.112	3.416SerVal: 3.416 ± 0.075	0.304SerTrp: 0.304 ± 0.021	3.068SerTyr: 3.068 ± 0.088	0.005SerXaa: 0.005 ± 0.003
Thr	1.097ThrAla: 1.097 ± 0.048	0.77ThrCys: 0.77 ± 0.032	2.415ThrAsp: 2.415 ± 0.058	2.907ThrGlu: 2.907 ± 0.062	2.506ThrPhe: 2.506 ± 0.063	1.831ThrGly: 1.831 ± 0.068	0.84ThrHis: 0.84 ± 0.039	4.635ThrIle: 4.635 ± 0.093	4.782ThrLys: 4.782 ± 0.093	4.241ThrLeu: 4.241 ± 0.077	0.772ThrMet: 0.772 ± 0.043	5.143ThrAsn: 5.143 ± 0.117	1.266ThrPro: 1.266 ± 0.045	1.19ThrGln: 1.19 ± 0.044	1.423ThrArg: 1.423 ± 0.05	3.9ThrSer: 3.9 ± 0.105	2.969ThrThr: 2.969 ± 0.1	2.086ThrVal: 2.086 ± 0.06	0.365ThrTrp: 0.365 ± 0.023	2.096ThrTyr: 2.096 ± 0.053	0.001ThrXaa: 0.001 ± 0.001
Val	1.229ValAla: 1.229 ± 0.046	1.142ValCys: 1.142 ± 0.046	3.444ValAsp: 3.444 ± 0.083	3.614ValGlu: 3.614 ± 0.071	2.54ValPhe: 2.54 ± 0.059	1.92ValGly: 1.92 ± 0.059	0.805ValHis: 0.805 ± 0.036	3.853ValIle: 3.853 ± 0.081	4.403ValLys: 4.403 ± 0.08	4.279ValLeu: 4.279 ± 0.065	0.976ValMet: 0.976 ± 0.044	4.101ValAsn: 4.101 ± 0.083	1.303ValPro: 1.303 ± 0.043	1.253ValGln: 1.253 ± 0.042	1.399ValArg: 1.399 ± 0.049	3.223ValSer: 3.223 ± 0.074	1.883ValThr: 1.883 ± 0.065	2.654ValVal: 2.654 ± 0.078	0.24ValTrp: 0.24 ± 0.017	2.288ValTyr: 2.288 ± 0.057	0.003ValXaa: 0.003 ± 0.002
Trp	0.132TrpAla: 0.132 ± 0.014	0.133TrpCys: 0.133 ± 0.013	0.375TrpAsp: 0.375 ± 0.025	0.323TrpGlu: 0.323 ± 0.022	0.291TrpPhe: 0.291 ± 0.018	0.18TrpGly: 0.18 ± 0.015	0.127TrpHis: 0.127 ± 0.014	0.562TrpIle: 0.562 ± 0.03	0.536TrpLys: 0.536 ± 0.029	0.445TrpLeu: 0.445 ± 0.026	0.132TrpMet: 0.132 ± 0.014	0.466TrpAsn: 0.466 ± 0.027	0.124TrpPro: 0.124 ± 0.013	0.117TrpGln: 0.117 ± 0.012	0.193TrpArg: 0.193 ± 0.019	0.4TrpSer: 0.4 ± 0.024	0.255TrpThr: 0.255 ± 0.02	0.201TrpVal: 0.201 ± 0.017	0.048TrpTrp: 0.048 ± 0.01	0.269TrpTyr: 0.269 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.025TyrAla: 1.025 ± 0.039	0.926TyrCys: 0.926 ± 0.038	3.003TyrAsp: 3.003 ± 0.064	3.139TyrGlu: 3.139 ± 0.067	2.583TyrPhe: 2.583 ± 0.067	1.721TyrGly: 1.721 ± 0.064	0.736TyrHis: 0.736 ± 0.029	4.719TyrIle: 4.719 ± 0.099	5.789TyrLys: 5.789 ± 0.104	4.673TyrLeu: 4.673 ± 0.095	0.998TyrMet: 0.998 ± 0.036	5.11TyrAsn: 5.11 ± 0.097	0.933TyrPro: 0.933 ± 0.038	1.048TyrGln: 1.048 ± 0.035	1.507TyrArg: 1.507 ± 0.049	2.959TyrSer: 2.959 ± 0.082	2.168TyrThr: 2.168 ± 0.05	2.443TyrVal: 2.443 ± 0.068	0.267TyrTrp: 0.267 ± 0.019	2.023TyrTyr: 2.023 ± 0.056	0.004TyrXaa: 0.004 ± 0.002
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.003XaaGlu: 0.003 ± 0.002	0.004XaaPhe: 0.004 ± 0.002	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.004XaaIle: 0.004 ± 0.002	0.007XaaLys: 0.007 ± 0.003	0.003XaaLeu: 0.003 ± 0.002	0.003XaaMet: 0.003 ± 0.002	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.003XaaGln: 0.003 ± 0.002	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.216XaaXaa: 0.216 ± 0.044

Statistics based on 2727 proteins (750327 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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