Methanothermobacter tenebrarum

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanothermobacter

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1556 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328PJB3|A0A328PJB3_9EURY Glutamyl-tRNA(Gln) amidotransferase subunit D OS=Methanothermobacter tenebrarum OX=680118 GN=gatD PE=3 SV=1
MM1 pKa = 8.21DD2 pKa = 5.03MLPLIKK8 pKa = 10.21VVPEE12 pKa = 3.96FNYY15 pKa = 10.94SLDD18 pKa = 3.52PSTGVIGAAVGRR30 pKa = 11.84EE31 pKa = 3.89LLILSMDD38 pKa = 4.82EE39 pKa = 3.9INEE42 pKa = 3.96QIAALEE48 pKa = 4.02ATADD52 pKa = 3.92DD53 pKa = 4.95LVNSLDD59 pKa = 3.77PSTVPEE65 pKa = 4.0GSYY68 pKa = 10.11PGRR71 pKa = 11.84EE72 pKa = 4.1GVYY75 pKa = 9.01LTAGKK80 pKa = 8.42LTNIVYY86 pKa = 9.91GFILGMILLCALIFLL101 pKa = 4.97

Molecular weight: 10.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328P9J5|A0A328P9J5_9EURY Transcriptional regulator OS=Methanothermobacter tenebrarum OX=680118 GN=DPC56_02750 PE=4 SV=1
MM1 pKa = 7.39SRR3 pKa = 11.84NKK5 pKa = 10.06HH6 pKa = 3.93VAKK9 pKa = 10.56KK10 pKa = 10.06IRR12 pKa = 11.84LSKK15 pKa = 10.46ARR17 pKa = 11.84KK18 pKa = 6.67QNRR21 pKa = 11.84RR22 pKa = 11.84VPVWVLLKK30 pKa = 9.6TNRR33 pKa = 11.84RR34 pKa = 11.84VLTHH38 pKa = 6.09PKK40 pKa = 7.61MRR42 pKa = 11.84HH43 pKa = 3.38WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.85LKK50 pKa = 10.03VV51 pKa = 2.95

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1556

0

1556

437150

37

1840

280.9

31.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.576 ± 0.056

1.157 ± 0.035

5.283 ± 0.039

7.985 ± 0.072

3.814 ± 0.051

7.101 ± 0.054

1.827 ± 0.029

10.15 ± 0.067

8.558 ± 0.085

9.366 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.027

3.918 ± 0.043

4.279 ± 0.035

2.067 ± 0.027

4.789 ± 0.046

5.236 ± 0.041

5.003 ± 0.052

6.159 ± 0.05

0.768 ± 0.024

3.431 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski