Amino acid dipepetide frequency for Circoviridae 16 LDMD-2013

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.935AlaAla: 2.935 ± 1.478
1.957AlaCys: 1.957 ± 1.496
1.957AlaAsp: 1.957 ± 0.909
1.957AlaGlu: 1.957 ± 0.684
2.935AlaPhe: 2.935 ± 1.299
4.892AlaGly: 4.892 ± 1.799
2.935AlaHis: 2.935 ± 0.538
0.978AlaIle: 0.978 ± 0.748
6.849AlaLys: 6.849 ± 2.321
2.935AlaLeu: 2.935 ± 1.196
0.978AlaMet: 0.978 ± 0.791
3.914AlaAsn: 3.914 ± 1.368
1.957AlaPro: 1.957 ± 1.401
3.914AlaGln: 3.914 ± 1.245
3.914AlaArg: 3.914 ± 0.965
5.871AlaSer: 5.871 ± 1.335
6.849AlaThr: 6.849 ± 0.94
3.914AlaVal: 3.914 ± 1.368
0.0AlaTrp: 0.0 ± 0.0
0.978AlaTyr: 0.978 ± 1.283
0.978AlaXaa: 0.978 ± 0.848
Cys
0.0CysAla: 0.0 ± 0.0
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.978CysGlu: 0.978 ± 0.748
1.957CysPhe: 1.957 ± 0.684
0.978CysGly: 0.978 ± 0.748
0.0CysHis: 0.0 ± 0.0
0.978CysIle: 0.978 ± 0.791
1.957CysLys: 1.957 ± 0.684
0.0CysLeu: 0.0 ± 0.0
0.978CysMet: 0.978 ± 0.748
0.0CysAsn: 0.0 ± 0.0
0.0CysPro: 0.0 ± 0.0
0.0CysGln: 0.0 ± 0.0
0.0CysArg: 0.0 ± 0.0
1.957CysSer: 1.957 ± 1.322
0.978CysThr: 0.978 ± 0.748
1.957CysVal: 1.957 ± 0.684
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
3.914AspAla: 3.914 ± 1.368
0.0AspCys: 0.0 ± 0.0
0.978AspAsp: 0.978 ± 0.848
1.957AspGlu: 1.957 ± 1.696
1.957AspPhe: 1.957 ± 0.909
5.871AspGly: 5.871 ± 1.077
0.0AspHis: 0.0 ± 0.0
1.957AspIle: 1.957 ± 1.582
0.978AspLys: 0.978 ± 0.748
3.914AspLeu: 3.914 ± 1.59
1.957AspMet: 1.957 ± 1.448
2.935AspAsn: 2.935 ± 1.478
1.957AspPro: 1.957 ± 1.582
1.957AspGln: 1.957 ± 0.909
0.0AspArg: 0.0 ± 0.0
1.957AspSer: 1.957 ± 0.909
4.892AspThr: 4.892 ± 0.892
6.849AspVal: 6.849 ± 3.431
1.957AspTrp: 1.957 ± 0.909
0.0AspTyr: 0.0 ± 0.0
0.0AspXaa: 0.0 ± 0.0
Glu
0.0GluAla: 0.0 ± 0.0
0.0GluCys: 0.0 ± 0.0
0.978GluAsp: 0.978 ± 0.848
5.871GluGlu: 5.871 ± 2.443
4.892GluPhe: 4.892 ± 3.172
0.978GluGly: 0.978 ± 0.848
0.0GluHis: 0.0 ± 0.0
0.0GluIle: 0.0 ± 0.0
3.914GluLys: 3.914 ± 1.632
2.935GluLeu: 2.935 ± 2.245
0.978GluMet: 0.978 ± 1.569
1.957GluAsn: 1.957 ± 2.565
2.935GluPro: 2.935 ± 2.549
0.0GluGln: 0.0 ± 0.0
0.0GluArg: 0.0 ± 0.0
3.914GluSer: 3.914 ± 1.874
2.935GluThr: 2.935 ± 1.723
5.871GluVal: 5.871 ± 3.581
1.957GluTrp: 1.957 ± 0.949
2.935GluTyr: 2.935 ± 1.839
0.0GluXaa: 0.0 ± 0.0
Phe
3.914PheAla: 3.914 ± 1.245
0.978PheCys: 0.978 ± 0.791
1.957PheAsp: 1.957 ± 1.696
1.957PheGlu: 1.957 ± 0.949
1.957PhePhe: 1.957 ± 1.696
2.935PheGly: 2.935 ± 1.928
0.0PheHis: 0.0 ± 0.0
1.957PheIle: 1.957 ± 2.565
2.935PheLys: 2.935 ± 1.57
0.978PheLeu: 0.978 ± 0.748
0.0PheMet: 0.0 ± 0.0
2.935PheAsn: 2.935 ± 1.276
0.978PhePro: 0.978 ± 1.283
4.892PheGln: 4.892 ± 2.323
1.957PheArg: 1.957 ± 1.696
0.978PheSer: 0.978 ± 0.791
1.957PheThr: 1.957 ± 1.374
1.957PheVal: 1.957 ± 1.374
0.978PheTrp: 0.978 ± 0.748
0.0PheTyr: 0.0 ± 0.0
0.0PheXaa: 0.0 ± 0.0
Gly
5.871GlyAla: 5.871 ± 1.404
0.0GlyCys: 0.0 ± 0.0
3.914GlyAsp: 3.914 ± 1.874
3.914GlyGlu: 3.914 ± 0.934
3.914GlyPhe: 3.914 ± 2.416
7.828GlyGly: 7.828 ± 2.666
0.978GlyHis: 0.978 ± 0.748
1.957GlyIle: 1.957 ± 1.496
4.892GlyLys: 4.892 ± 2.366
8.806GlyLeu: 8.806 ± 2.476
1.957GlyMet: 1.957 ± 1.298
3.914GlyAsn: 3.914 ± 1.368
4.892GlyPro: 4.892 ± 2.119
2.935GlyGln: 2.935 ± 2.544
2.935GlyArg: 2.935 ± 2.495
1.957GlySer: 1.957 ± 1.322
5.871GlyThr: 5.871 ± 1.774
7.828GlyVal: 7.828 ± 2.154
0.0GlyTrp: 0.0 ± 0.0
2.935GlyTyr: 2.935 ± 1.57
0.978GlyXaa: 0.978 ± 1.283
His
1.957HisAla: 1.957 ± 0.684
0.978HisCys: 0.978 ± 0.748
2.935HisAsp: 2.935 ± 1.484
0.978HisGlu: 0.978 ± 1.283
0.978HisPhe: 0.978 ± 0.848
3.914HisGly: 3.914 ± 0.934
0.978HisHis: 0.978 ± 0.748
0.978HisIle: 0.978 ± 0.748
0.978HisLys: 0.978 ± 0.848
1.957HisLeu: 1.957 ± 1.322
0.978HisMet: 0.978 ± 0.848
0.0HisAsn: 0.0 ± 0.0
0.0HisPro: 0.0 ± 0.0
1.957HisGln: 1.957 ± 1.401
0.978HisArg: 0.978 ± 0.748
0.0HisSer: 0.0 ± 0.0
0.978HisThr: 0.978 ± 0.748
4.892HisVal: 4.892 ± 1.799
0.0HisTrp: 0.0 ± 0.0
0.0HisTyr: 0.0 ± 0.0
0.0HisXaa: 0.0 ± 0.0
Ile
0.0IleAla: 0.0 ± 0.0
0.0IleCys: 0.0 ± 0.0
2.935IleAsp: 2.935 ± 1.57
0.978IleGlu: 0.978 ± 0.848
0.978IlePhe: 0.978 ± 0.748
4.892IleGly: 4.892 ± 1.843
1.957IleHis: 1.957 ± 1.582
0.0IleIle: 0.0 ± 0.0
1.957IleLys: 1.957 ± 1.322
3.914IleLeu: 3.914 ± 2.15
1.957IleMet: 1.957 ± 1.582
1.957IleAsn: 1.957 ± 0.909
0.978IlePro: 0.978 ± 0.848
2.935IleGln: 2.935 ± 2.374
2.935IleArg: 2.935 ± 1.484
1.957IleSer: 1.957 ± 1.582
0.978IleThr: 0.978 ± 0.748
3.914IleVal: 3.914 ± 0.934
1.957IleTrp: 1.957 ± 1.374
0.978IleTyr: 0.978 ± 0.848
0.0IleXaa: 0.0 ± 0.0
Lys
4.892LysAla: 4.892 ± 1.843
0.0LysCys: 0.0 ± 0.0
3.914LysAsp: 3.914 ± 0.965
1.957LysGlu: 1.957 ± 1.374
0.978LysPhe: 0.978 ± 0.848
4.892LysGly: 4.892 ± 0.904
0.978LysHis: 0.978 ± 1.283
7.828LysIle: 7.828 ± 2.61
1.957LysLys: 1.957 ± 1.582
1.957LysLeu: 1.957 ± 1.374
0.0LysMet: 0.0 ± 0.0
3.914LysAsn: 3.914 ± 3.165
2.935LysPro: 2.935 ± 1.57
1.957LysGln: 1.957 ± 2.565
7.828LysArg: 7.828 ± 2.49
3.914LysSer: 3.914 ± 1.818
3.914LysThr: 3.914 ± 1.431
6.849LysVal: 6.849 ± 1.496
0.978LysTrp: 0.978 ± 0.848
3.914LysTyr: 3.914 ± 1.632
0.0LysXaa: 0.0 ± 0.0
Leu
7.828LeuAla: 7.828 ± 3.744
2.935LeuCys: 2.935 ± 2.245
3.914LeuAsp: 3.914 ± 1.898
2.935LeuGlu: 2.935 ± 2.245
2.935LeuPhe: 2.935 ± 1.298
6.849LeuGly: 6.849 ± 2.445
1.957LeuHis: 1.957 ± 1.496
1.957LeuIle: 1.957 ± 0.949
8.806LeuLys: 8.806 ± 3.587
7.828LeuLeu: 7.828 ± 3.015
0.0LeuMet: 0.0 ± 0.0
3.914LeuAsn: 3.914 ± 1.332
2.935LeuPro: 2.935 ± 1.57
3.914LeuGln: 3.914 ± 2.476
8.806LeuArg: 8.806 ± 2.518
8.806LeuSer: 8.806 ± 2.412
2.935LeuThr: 2.935 ± 1.637
5.871LeuVal: 5.871 ± 3.321
0.0LeuTrp: 0.0 ± 0.0
1.957LeuTyr: 1.957 ± 1.582
0.0LeuXaa: 0.0 ± 0.0
Met
1.957MetAla: 1.957 ± 0.909
0.978MetCys: 0.978 ± 0.791
0.0MetAsp: 0.0 ± 0.0
0.0MetGlu: 0.0 ± 0.0
0.0MetPhe: 0.0 ± 0.0
0.0MetGly: 0.0 ± 0.0
0.0MetHis: 0.0 ± 0.0
0.0MetIle: 0.0 ± 0.0
1.957MetLys: 1.957 ± 0.909
1.957MetLeu: 1.957 ± 1.696
0.0MetMet: 0.0 ± 0.0
0.978MetAsn: 0.978 ± 0.791
1.957MetPro: 1.957 ± 1.374
0.0MetGln: 0.0 ± 0.0
0.978MetArg: 0.978 ± 0.748
5.871MetSer: 5.871 ± 1.963
1.957MetThr: 1.957 ± 0.909
0.0MetVal: 0.0 ± 0.0
0.0MetTrp: 0.0 ± 0.0
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
2.935AsnAla: 2.935 ± 1.276
0.978AsnCys: 0.978 ± 0.748
2.935AsnAsp: 2.935 ± 2.374
0.978AsnGlu: 0.978 ± 0.791
3.914AsnPhe: 3.914 ± 2.476
2.935AsnGly: 2.935 ± 1.128
0.978AsnHis: 0.978 ± 0.848
1.957AsnIle: 1.957 ± 1.582
3.914AsnLys: 3.914 ± 1.818
5.871AsnLeu: 5.871 ± 2.323
0.978AsnMet: 0.978 ± 0.791
4.892AsnAsn: 4.892 ± 2.019
0.0AsnPro: 0.0 ± 0.0
0.0AsnGln: 0.0 ± 0.0
4.892AsnArg: 4.892 ± 0.974
2.935AsnSer: 2.935 ± 1.128
5.871AsnThr: 5.871 ± 1.077
4.892AsnVal: 4.892 ± 2.591
0.978AsnTrp: 0.978 ± 0.848
2.935AsnTyr: 2.935 ± 1.839
0.0AsnXaa: 0.0 ± 0.0
Pro
1.957ProAla: 1.957 ± 1.322
0.0ProCys: 0.0 ± 0.0
1.957ProAsp: 1.957 ± 0.684
2.935ProGlu: 2.935 ± 1.57
1.957ProPhe: 1.957 ± 1.696
1.957ProGly: 1.957 ± 1.401
1.957ProHis: 1.957 ± 1.401
1.957ProIle: 1.957 ± 1.496
2.935ProLys: 2.935 ± 2.538
4.892ProLeu: 4.892 ± 1.68
0.978ProMet: 0.978 ± 0.791
1.957ProAsn: 1.957 ± 1.696
2.935ProPro: 2.935 ± 2.549
0.0ProGln: 0.0 ± 0.0
0.978ProArg: 0.978 ± 0.791
2.935ProSer: 2.935 ± 0.538
4.892ProThr: 4.892 ± 1.245
1.957ProVal: 1.957 ± 1.696
0.978ProTrp: 0.978 ± 1.283
4.892ProTyr: 4.892 ± 2.535
0.0ProXaa: 0.0 ± 0.0
Gln
3.914GlnAla: 3.914 ± 1.818
0.0GlnCys: 0.0 ± 0.0
1.957GlnAsp: 1.957 ± 1.374
0.0GlnGlu: 0.0 ± 0.0
0.0GlnPhe: 0.0 ± 0.0
3.914GlnGly: 3.914 ± 2.416
1.957GlnHis: 1.957 ± 0.909
0.978GlnIle: 0.978 ± 0.848
3.914GlnLys: 3.914 ± 1.818
2.935GlnLeu: 2.935 ± 1.57
0.0GlnMet: 0.0 ± 0.0
0.978GlnAsn: 0.978 ± 0.748
2.935GlnPro: 2.935 ± 2.374
0.978GlnGln: 0.978 ± 1.283
3.914GlnArg: 3.914 ± 0.965
0.0GlnSer: 0.0 ± 0.0
2.935GlnThr: 2.935 ± 1.478
2.935GlnVal: 2.935 ± 1.723
0.0GlnTrp: 0.0 ± 0.0
0.978GlnTyr: 0.978 ± 0.791
0.0GlnXaa: 0.0 ± 0.0
Arg
3.914ArgAla: 3.914 ± 2.19
0.0ArgCys: 0.0 ± 0.0
1.957ArgAsp: 1.957 ± 0.684
0.978ArgGlu: 0.978 ± 0.748
0.978ArgPhe: 0.978 ± 0.748
3.914ArgGly: 3.914 ± 0.934
1.957ArgHis: 1.957 ± 1.496
5.871ArgIle: 5.871 ± 1.436
3.914ArgLys: 3.914 ± 0.965
7.828ArgLeu: 7.828 ± 0.683
0.978ArgMet: 0.978 ± 0.848
2.935ArgAsn: 2.935 ± 1.128
0.978ArgPro: 0.978 ± 0.791
2.935ArgGln: 2.935 ± 1.276
7.828ArgArg: 7.828 ± 3.572
3.914ArgSer: 3.914 ± 1.71
6.849ArgThr: 6.849 ± 2.241
7.828ArgVal: 7.828 ± 1.311
0.978ArgTrp: 0.978 ± 0.791
0.0ArgTyr: 0.0 ± 0.0
0.978ArgXaa: 0.978 ± 0.791
Ser
2.935SerAla: 2.935 ± 1.276
0.978SerCys: 0.978 ± 0.791
2.935SerAsp: 2.935 ± 1.478
3.914SerGlu: 3.914 ± 1.71
1.957SerPhe: 1.957 ± 0.684
5.871SerGly: 5.871 ± 2.564
1.957SerHis: 1.957 ± 1.496
3.914SerIle: 3.914 ± 1.818
5.871SerLys: 5.871 ± 1.568
4.892SerLeu: 4.892 ± 2.857
1.957SerMet: 1.957 ± 0.869
3.914SerAsn: 3.914 ± 1.632
2.935SerPro: 2.935 ± 2.544
1.957SerGln: 1.957 ± 0.909
4.892SerArg: 4.892 ± 2.323
10.763SerSer: 10.763 ± 1.312
5.871SerThr: 5.871 ± 1.335
2.935SerVal: 2.935 ± 2.374
0.0SerTrp: 0.0 ± 0.0
0.0SerTyr: 0.0 ± 0.0
0.978SerXaa: 0.978 ± 0.748
Thr
8.806ThrAla: 8.806 ± 1.213
0.0ThrCys: 0.0 ± 0.0
5.871ThrAsp: 5.871 ± 2.443
3.914ThrGlu: 3.914 ± 2.4
0.978ThrPhe: 0.978 ± 0.791
5.871ThrGly: 5.871 ± 1.135
2.935ThrHis: 2.935 ± 1.723
2.935ThrIle: 2.935 ± 0.538
3.914ThrLys: 3.914 ± 1.632
3.914ThrLeu: 3.914 ± 1.874
0.978ThrMet: 0.978 ± 0.791
5.871ThrAsn: 5.871 ± 1.755
2.935ThrPro: 2.935 ± 1.723
0.978ThrGln: 0.978 ± 0.748
3.914ThrArg: 3.914 ± 0.832
5.871ThrSer: 5.871 ± 1.404
1.957ThrThr: 1.957 ± 1.374
2.935ThrVal: 2.935 ± 2.245
0.978ThrTrp: 0.978 ± 0.791
1.957ThrTyr: 1.957 ± 0.909
0.0ThrXaa: 0.0 ± 0.0
Val
2.935ValAla: 2.935 ± 2.245
0.978ValCys: 0.978 ± 0.748
3.914ValAsp: 3.914 ± 1.874
3.914ValGlu: 3.914 ± 2.304
0.978ValPhe: 0.978 ± 1.283
7.828ValGly: 7.828 ± 1.869
3.914ValHis: 3.914 ± 2.15
0.0ValIle: 0.0 ± 0.0
2.935ValLys: 2.935 ± 1.478
13.699ValLeu: 13.699 ± 4.987
0.978ValMet: 0.978 ± 0.848
6.849ValAsn: 6.849 ± 1.496
6.849ValPro: 6.849 ± 1.072
1.957ValGln: 1.957 ± 0.684
7.828ValArg: 7.828 ± 2.977
6.849ValSer: 6.849 ± 2.389
1.957ValThr: 1.957 ± 1.374
15.656ValVal: 15.656 ± 7.995
0.978ValTrp: 0.978 ± 0.748
2.935ValTyr: 2.935 ± 0.538
0.0ValXaa: 0.0 ± 0.0
Trp
0.978TrpAla: 0.978 ± 1.283
0.978TrpCys: 0.978 ± 0.791
0.0TrpAsp: 0.0 ± 0.0
1.957TrpGlu: 1.957 ± 1.401
0.978TrpPhe: 0.978 ± 0.848
0.0TrpGly: 0.0 ± 0.0
0.0TrpHis: 0.0 ± 0.0
0.978TrpIle: 0.978 ± 0.748
0.978TrpLys: 0.978 ± 0.748
0.978TrpLeu: 0.978 ± 0.748
0.0TrpMet: 0.0 ± 0.0
0.0TrpAsn: 0.0 ± 0.0
0.0TrpPro: 0.0 ± 0.0
0.0TrpGln: 0.0 ± 0.0
0.978TrpArg: 0.978 ± 0.791
0.978TrpSer: 0.978 ± 0.848
1.957TrpThr: 1.957 ± 1.374
0.0TrpVal: 0.0 ± 0.0
0.0TrpTrp: 0.0 ± 0.0
1.957TrpTyr: 1.957 ± 0.909
0.0TrpXaa: 0.0 ± 0.0
Tyr
0.0TyrAla: 0.0 ± 0.0
0.978TyrCys: 0.978 ± 1.283
0.978TyrAsp: 0.978 ± 1.283
1.957TyrGlu: 1.957 ± 0.949
0.978TyrPhe: 0.978 ± 0.791
1.957TyrGly: 1.957 ± 1.374
0.978TyrHis: 0.978 ± 0.848
0.978TyrIle: 0.978 ± 0.791
0.0TyrLys: 0.0 ± 0.0
4.892TyrLeu: 4.892 ± 2.943
0.978TyrMet: 0.978 ± 0.848
1.957TyrAsn: 1.957 ± 1.582
1.957TyrPro: 1.957 ± 1.696
1.957TyrGln: 1.957 ± 0.909
1.957TyrArg: 1.957 ± 1.374
0.0TyrSer: 0.0 ± 0.0
0.978TyrThr: 0.978 ± 0.791
4.892TyrVal: 4.892 ± 2.211
0.978TyrTrp: 0.978 ± 1.283
0.978TyrTyr: 0.978 ± 0.791
0.0TyrXaa: 0.0 ± 0.0
Xaa
1.957XaaAla: 1.957 ± 0.684
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
1.957XaaPro: 1.957 ± 1.401
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4 proteins (1023 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski