Nitratireductor pacificus pht-3B

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Nitratireductor; Nitratireductor pacificus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K2N2A7|K2N2A7_9RHIZ UvrABC system protein A OS=Nitratireductor pacificus pht-3B OX=391937 GN=uvrA PE=3 SV=1
MM1 pKa = 7.36TKK3 pKa = 8.51ITYY6 pKa = 9.29IAPNGSQFDD15 pKa = 3.47VDD17 pKa = 4.15ADD19 pKa = 3.72NGSTVMEE26 pKa = 4.03NAIRR30 pKa = 11.84NAVPGIEE37 pKa = 4.22AEE39 pKa = 4.31CGGACACATCHH50 pKa = 6.69VYY52 pKa = 10.55VDD54 pKa = 4.4PDD56 pKa = 3.19WAEE59 pKa = 3.73AAGEE63 pKa = 4.12PEE65 pKa = 4.51AMEE68 pKa = 5.17EE69 pKa = 4.62DD70 pKa = 3.57MLDD73 pKa = 3.47FAFEE77 pKa = 4.14VQPTSRR83 pKa = 11.84LSCQIIVRR91 pKa = 11.84EE92 pKa = 4.07EE93 pKa = 3.87LDD95 pKa = 3.56GLVVRR100 pKa = 11.84IPEE103 pKa = 4.09RR104 pKa = 11.84QAA106 pKa = 3.15

Molecular weight: 11.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K2MJ28|K2MJ28_9RHIZ Transporter OS=Nitratireductor pacificus pht-3B OX=391937 GN=NA2_02884 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.09QPSKK9 pKa = 8.8IVRR12 pKa = 11.84KK13 pKa = 9.24RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84MATTGGRR28 pKa = 11.84RR29 pKa = 11.84VIAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4196

0

4196

1318631

31

3422

314.3

34.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.906 ± 0.048

0.797 ± 0.013

5.63 ± 0.029

5.972 ± 0.037

3.877 ± 0.023

8.896 ± 0.037

2.003 ± 0.019

5.197 ± 0.029

3.001 ± 0.031

10.196 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.016

2.48 ± 0.021

5.02 ± 0.024

2.89 ± 0.02

7.223 ± 0.036

5.228 ± 0.029

5.178 ± 0.028

7.433 ± 0.032

1.247 ± 0.014

2.189 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski