Amino acid dipepetide frequency for Streptomyces variegatus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.353AlaAla: 20.353 ± 0.136
1.08AlaCys: 1.08 ± 0.023
8.086AlaAsp: 8.086 ± 0.072
8.636AlaGlu: 8.636 ± 0.092
3.579AlaPhe: 3.579 ± 0.042
13.125AlaGly: 13.125 ± 0.095
2.865AlaHis: 2.865 ± 0.038
3.412AlaIle: 3.412 ± 0.032
2.858AlaLys: 2.858 ± 0.046
14.477AlaLeu: 14.477 ± 0.113
2.493AlaMet: 2.493 ± 0.036
1.868AlaAsn: 1.868 ± 0.029
6.89AlaPro: 6.89 ± 0.068
3.758AlaGln: 3.758 ± 0.044
10.217AlaArg: 10.217 ± 0.089
5.917AlaSer: 5.917 ± 0.046
6.774AlaThr: 6.774 ± 0.06
12.14AlaVal: 12.14 ± 0.087
1.846AlaTrp: 1.846 ± 0.032
2.801AlaTyr: 2.801 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
1.063CysAla: 1.063 ± 0.023
0.088CysCys: 0.088 ± 0.007
0.478CysAsp: 0.478 ± 0.015
0.425CysGlu: 0.425 ± 0.016
0.217CysPhe: 0.217 ± 0.01
0.955CysGly: 0.955 ± 0.022
0.188CysHis: 0.188 ± 0.009
0.161CysIle: 0.161 ± 0.007
0.111CysLys: 0.111 ± 0.008
0.763CysLeu: 0.763 ± 0.018
0.117CysMet: 0.117 ± 0.007
0.126CysAsn: 0.126 ± 0.008
0.464CysPro: 0.464 ± 0.014
0.157CysGln: 0.157 ± 0.008
0.6CysArg: 0.6 ± 0.017
0.419CysSer: 0.419 ± 0.014
0.49CysThr: 0.49 ± 0.015
0.674CysVal: 0.674 ± 0.018
0.128CysTrp: 0.128 ± 0.007
0.148CysTyr: 0.148 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.432AspAla: 7.432 ± 0.065
0.412AspCys: 0.412 ± 0.013
3.682AspAsp: 3.682 ± 0.048
3.923AspGlu: 3.923 ± 0.048
1.683AspPhe: 1.683 ± 0.026
6.548AspGly: 6.548 ± 0.062
1.422AspHis: 1.422 ± 0.022
1.906AspIle: 1.906 ± 0.03
1.289AspLys: 1.289 ± 0.03
6.196AspLeu: 6.196 ± 0.056
0.866AspMet: 0.866 ± 0.019
0.982AspAsn: 0.982 ± 0.024
4.494AspPro: 4.494 ± 0.052
1.549AspGln: 1.549 ± 0.026
4.931AspArg: 4.931 ± 0.053
2.564AspSer: 2.564 ± 0.035
3.324AspThr: 3.324 ± 0.035
4.813AspVal: 4.813 ± 0.046
1.06AspTrp: 1.06 ± 0.023
1.121AspTyr: 1.121 ± 0.021
0.0AspXaa: 0.0 ± 0.0
Glu
7.501GluAla: 7.501 ± 0.076
0.364GluCys: 0.364 ± 0.012
2.938GluAsp: 2.938 ± 0.038
3.673GluGlu: 3.673 ± 0.051
1.458GluPhe: 1.458 ± 0.026
4.442GluGly: 4.442 ± 0.041
1.526GluHis: 1.526 ± 0.026
2.272GluIle: 2.272 ± 0.035
1.533GluLys: 1.533 ± 0.031
6.731GluLeu: 6.731 ± 0.067
0.88GluMet: 0.88 ± 0.021
1.076GluAsn: 1.076 ± 0.022
3.456GluPro: 3.456 ± 0.045
2.399GluGln: 2.399 ± 0.033
5.421GluArg: 5.421 ± 0.066
2.546GluSer: 2.546 ± 0.032
2.948GluThr: 2.948 ± 0.036
4.429GluVal: 4.429 ± 0.049
0.779GluTrp: 0.779 ± 0.02
1.123GluTyr: 1.123 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
3.693PheAla: 3.693 ± 0.036
0.265PheCys: 0.265 ± 0.011
1.972PheAsp: 1.972 ± 0.033
1.498PheGlu: 1.498 ± 0.026
0.91PhePhe: 0.91 ± 0.025
3.049PheGly: 3.049 ± 0.04
0.628PheHis: 0.628 ± 0.019
0.71PheIle: 0.71 ± 0.02
0.54PheLys: 0.54 ± 0.015
2.609PheLeu: 2.609 ± 0.041
0.41PheMet: 0.41 ± 0.013
0.586PheAsn: 0.586 ± 0.016
1.378PhePro: 1.378 ± 0.024
0.728PheGln: 0.728 ± 0.018
1.844PheArg: 1.844 ± 0.029
1.493PheSer: 1.493 ± 0.026
2.064PheThr: 2.064 ± 0.029
2.271PheVal: 2.271 ± 0.034
0.453PheTrp: 0.453 ± 0.014
0.609PheTyr: 0.609 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
10.816GlyAla: 10.816 ± 0.075
0.858GlyCys: 0.858 ± 0.02
5.194GlyAsp: 5.194 ± 0.054
5.261GlyGlu: 5.261 ± 0.047
2.946GlyPhe: 2.946 ± 0.042
8.744GlyGly: 8.744 ± 0.089
2.391GlyHis: 2.391 ± 0.031
3.518GlyIle: 3.518 ± 0.045
2.59GlyLys: 2.59 ± 0.042
9.572GlyLeu: 9.572 ± 0.07
2.024GlyMet: 2.024 ± 0.032
1.748GlyAsn: 1.748 ± 0.036
5.205GlyPro: 5.205 ± 0.049
2.776GlyGln: 2.776 ± 0.038
7.796GlyArg: 7.796 ± 0.067
5.36GlySer: 5.36 ± 0.051
6.526GlyThr: 6.526 ± 0.066
7.665GlyVal: 7.665 ± 0.057
1.725GlyTrp: 1.725 ± 0.028
2.293GlyTyr: 2.293 ± 0.035
0.0GlyXaa: 0.0 ± 0.0
His
2.686HisAla: 2.686 ± 0.04
0.21HisCys: 0.21 ± 0.009
1.407HisAsp: 1.407 ± 0.028
1.258HisGlu: 1.258 ± 0.027
0.649HisPhe: 0.649 ± 0.017
2.425HisGly: 2.425 ± 0.029
0.694HisHis: 0.694 ± 0.021
0.689HisIle: 0.689 ± 0.018
0.387HisLys: 0.387 ± 0.013
2.428HisLeu: 2.428 ± 0.033
0.333HisMet: 0.333 ± 0.011
0.363HisAsn: 0.363 ± 0.012
1.861HisPro: 1.861 ± 0.029
0.645HisGln: 0.645 ± 0.017
2.122HisArg: 2.122 ± 0.031
1.003HisSer: 1.003 ± 0.025
1.411HisThr: 1.411 ± 0.023
1.728HisVal: 1.728 ± 0.029
0.374HisTrp: 0.374 ± 0.014
0.484HisTyr: 0.484 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.767IleAla: 4.767 ± 0.053
0.273IleCys: 0.273 ± 0.011
2.258IleAsp: 2.258 ± 0.033
1.964IleGlu: 1.964 ± 0.028
0.719IlePhe: 0.719 ± 0.018
3.6IleGly: 3.6 ± 0.04
0.621IleHis: 0.621 ± 0.015
0.94IleIle: 0.94 ± 0.024
0.758IleLys: 0.758 ± 0.021
2.463IleLeu: 2.463 ± 0.035
0.467IleMet: 0.467 ± 0.014
0.693IleAsn: 0.693 ± 0.019
1.788IlePro: 1.788 ± 0.025
0.715IleGln: 0.715 ± 0.018
2.251IleArg: 2.251 ± 0.032
1.654IleSer: 1.654 ± 0.031
2.241IleThr: 2.241 ± 0.033
2.745IleVal: 2.745 ± 0.039
0.36IleTrp: 0.36 ± 0.011
0.524IleTyr: 0.524 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
3.092LysAla: 3.092 ± 0.046
0.112LysCys: 0.112 ± 0.006
1.416LysAsp: 1.416 ± 0.029
1.214LysGlu: 1.214 ± 0.027
0.477LysPhe: 0.477 ± 0.016
1.976LysGly: 1.976 ± 0.037
0.437LysHis: 0.437 ± 0.015
0.88LysIle: 0.88 ± 0.023
0.946LysLys: 0.946 ± 0.026
2.086LysLeu: 2.086 ± 0.036
0.409LysMet: 0.409 ± 0.013
0.548LysAsn: 0.548 ± 0.017
1.44LysPro: 1.44 ± 0.028
0.784LysGln: 0.784 ± 0.021
1.484LysArg: 1.484 ± 0.027
1.202LysSer: 1.202 ± 0.025
1.351LysThr: 1.351 ± 0.024
2.012LysVal: 2.012 ± 0.039
0.287LysTrp: 0.287 ± 0.012
0.501LysTyr: 0.501 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
14.838LeuAla: 14.838 ± 0.103
0.833LeuCys: 0.833 ± 0.019
6.705LeuAsp: 6.705 ± 0.058
4.771LeuGlu: 4.771 ± 0.052
2.668LeuPhe: 2.668 ± 0.041
9.234LeuGly: 9.234 ± 0.077
2.338LeuHis: 2.338 ± 0.035
3.308LeuIle: 3.308 ± 0.043
2.177LeuLys: 2.177 ± 0.035
11.301LeuLeu: 11.301 ± 0.112
1.687LeuMet: 1.687 ± 0.03
1.702LeuAsn: 1.702 ± 0.029
6.487LeuPro: 6.487 ± 0.051
2.15LeuGln: 2.15 ± 0.029
8.567LeuArg: 8.567 ± 0.078
5.326LeuSer: 5.326 ± 0.043
7.047LeuThr: 7.047 ± 0.059
8.92LeuVal: 8.92 ± 0.082
1.335LeuTrp: 1.335 ± 0.024
1.904LeuTyr: 1.904 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.305MetAla: 2.305 ± 0.031
0.144MetCys: 0.144 ± 0.008
0.908MetAsp: 0.908 ± 0.02
0.759MetGlu: 0.759 ± 0.021
0.455MetPhe: 0.455 ± 0.015
1.343MetGly: 1.343 ± 0.025
0.358MetHis: 0.358 ± 0.012
0.682MetIle: 0.682 ± 0.015
0.426MetLys: 0.426 ± 0.012
1.678MetLeu: 1.678 ± 0.026
0.308MetMet: 0.308 ± 0.012
0.455MetAsn: 0.455 ± 0.016
1.153MetPro: 1.153 ± 0.021
0.445MetGln: 0.445 ± 0.016
1.469MetArg: 1.469 ± 0.023
1.314MetSer: 1.314 ± 0.024
1.606MetThr: 1.606 ± 0.027
1.3MetVal: 1.3 ± 0.024
0.225MetTrp: 0.225 ± 0.01
0.34MetTyr: 0.34 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.211AsnAla: 2.211 ± 0.037
0.161AsnCys: 0.161 ± 0.009
0.975AsnAsp: 0.975 ± 0.021
0.807AsnGlu: 0.807 ± 0.018
0.498AsnPhe: 0.498 ± 0.014
1.88AsnGly: 1.88 ± 0.038
0.394AsnHis: 0.394 ± 0.014
0.686AsnIle: 0.686 ± 0.016
0.423AsnLys: 0.423 ± 0.015
1.67AsnLeu: 1.67 ± 0.027
0.307AsnMet: 0.307 ± 0.011
0.445AsnAsn: 0.445 ± 0.017
1.367AsnPro: 1.367 ± 0.023
0.54AsnGln: 0.54 ± 0.016
1.249AsnArg: 1.249 ± 0.022
0.961AsnSer: 0.961 ± 0.023
1.135AsnThr: 1.135 ± 0.026
1.396AsnVal: 1.396 ± 0.025
0.321AsnTrp: 0.321 ± 0.011
0.434AsnTyr: 0.434 ± 0.013
0.0AsnXaa: 0.0 ± 0.0
Pro
8.553ProAla: 8.553 ± 0.074
0.328ProCys: 0.328 ± 0.013
4.477ProAsp: 4.477 ± 0.045
4.486ProGlu: 4.486 ± 0.052
1.564ProPhe: 1.564 ± 0.026
6.763ProGly: 6.763 ± 0.067
1.447ProHis: 1.447 ± 0.027
1.257ProIle: 1.257 ± 0.022
1.27ProLys: 1.27 ± 0.026
5.387ProLeu: 5.387 ± 0.047
0.997ProMet: 0.997 ± 0.02
0.878ProAsn: 0.878 ± 0.019
3.434ProPro: 3.434 ± 0.056
1.822ProGln: 1.822 ± 0.031
3.832ProArg: 3.832 ± 0.045
3.127ProSer: 3.127 ± 0.04
3.061ProThr: 3.061 ± 0.043
5.509ProVal: 5.509 ± 0.047
0.915ProTrp: 0.915 ± 0.024
1.446ProTyr: 1.446 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.817GlnAla: 3.817 ± 0.041
0.172GlnCys: 0.172 ± 0.009
1.513GlnAsp: 1.513 ± 0.025
1.538GlnGlu: 1.538 ± 0.025
0.69GlnPhe: 0.69 ± 0.016
2.43GlnGly: 2.43 ± 0.035
0.645GlnHis: 0.645 ± 0.016
1.109GlnIle: 1.109 ± 0.024
0.673GlnLys: 0.673 ± 0.021
2.905GlnLeu: 2.905 ± 0.038
0.517GlnMet: 0.517 ± 0.016
0.506GlnAsn: 0.506 ± 0.016
1.75GlnPro: 1.75 ± 0.036
1.234GlnGln: 1.234 ± 0.028
2.361GlnArg: 2.361 ± 0.036
1.249GlnSer: 1.249 ± 0.024
1.367GlnThr: 1.367 ± 0.023
2.394GlnVal: 2.394 ± 0.034
0.494GlnTrp: 0.494 ± 0.016
0.631GlnTyr: 0.631 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
9.627ArgAla: 9.627 ± 0.083
0.564ArgCys: 0.564 ± 0.017
4.31ArgAsp: 4.31 ± 0.043
4.817ArgGlu: 4.817 ± 0.056
2.332ArgPhe: 2.332 ± 0.034
5.773ArgGly: 5.773 ± 0.054
2.16ArgHis: 2.16 ± 0.032
3.239ArgIle: 3.239 ± 0.035
1.699ArgLys: 1.699 ± 0.03
8.876ArgLeu: 8.876 ± 0.092
1.762ArgMet: 1.762 ± 0.027
1.376ArgAsn: 1.376 ± 0.031
4.929ArgPro: 4.929 ± 0.056
2.323ArgGln: 2.323 ± 0.029
7.565ArgArg: 7.565 ± 0.081
4.014ArgSer: 4.014 ± 0.044
5.297ArgThr: 5.297 ± 0.052
5.892ArgVal: 5.892 ± 0.052
1.392ArgTrp: 1.392 ± 0.028
1.8ArgTyr: 1.8 ± 0.026
0.0ArgXaa: 0.0 ± 0.0
Ser
6.534SerAla: 6.534 ± 0.057
0.386SerCys: 0.386 ± 0.013
2.732SerAsp: 2.732 ± 0.036
2.451SerGlu: 2.451 ± 0.034
1.54SerPhe: 1.54 ± 0.029
6.074SerGly: 6.074 ± 0.052
1.047SerHis: 1.047 ± 0.025
1.357SerIle: 1.357 ± 0.026
1.043SerLys: 1.043 ± 0.023
4.812SerLeu: 4.812 ± 0.045
1.086SerMet: 1.086 ± 0.02
0.889SerAsn: 0.889 ± 0.019
3.168SerPro: 3.168 ± 0.039
1.243SerGln: 1.243 ± 0.024
3.685SerArg: 3.685 ± 0.044
2.794SerSer: 2.794 ± 0.037
3.011SerThr: 3.011 ± 0.036
4.354SerVal: 4.354 ± 0.043
0.91SerTrp: 0.91 ± 0.022
1.245SerTyr: 1.245 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
8.875ThrAla: 8.875 ± 0.073
0.423ThrCys: 0.423 ± 0.015
3.688ThrAsp: 3.688 ± 0.042
3.264ThrGlu: 3.264 ± 0.039
1.631ThrPhe: 1.631 ± 0.024
6.821ThrGly: 6.821 ± 0.058
1.251ThrHis: 1.251 ± 0.022
1.705ThrIle: 1.705 ± 0.029
1.237ThrLys: 1.237 ± 0.025
5.806ThrLeu: 5.806 ± 0.047
0.969ThrMet: 0.969 ± 0.019
1.025ThrAsn: 1.025 ± 0.021
4.109ThrPro: 4.109 ± 0.05
1.372ThrGln: 1.372 ± 0.023
3.977ThrArg: 3.977 ± 0.044
3.151ThrSer: 3.151 ± 0.039
3.86ThrThr: 3.86 ± 0.048
6.124ThrVal: 6.124 ± 0.054
0.944ThrTrp: 0.944 ± 0.02
1.427ThrTyr: 1.427 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
10.552ValAla: 10.552 ± 0.076
0.77ValCys: 0.77 ± 0.019
4.974ValAsp: 4.974 ± 0.047
4.739ValGlu: 4.739 ± 0.048
2.493ValPhe: 2.493 ± 0.034
6.384ValGly: 6.384 ± 0.06
1.988ValHis: 1.988 ± 0.027
2.911ValIle: 2.911 ± 0.039
1.856ValLys: 1.856 ± 0.034
9.646ValLeu: 9.646 ± 0.088
1.462ValMet: 1.462 ± 0.026
1.713ValAsn: 1.713 ± 0.032
5.325ValPro: 5.325 ± 0.052
2.088ValGln: 2.088 ± 0.028
7.195ValArg: 7.195 ± 0.063
4.361ValSer: 4.361 ± 0.044
5.817ValThr: 5.817 ± 0.053
8.015ValVal: 8.015 ± 0.066
1.191ValTrp: 1.191 ± 0.024
1.633ValTyr: 1.633 ± 0.027
0.0ValXaa: 0.0 ± 0.0
Trp
1.669TrpAla: 1.669 ± 0.026
0.151TrpCys: 0.151 ± 0.009
0.853TrpAsp: 0.853 ± 0.021
0.735TrpGlu: 0.735 ± 0.019
0.536TrpPhe: 0.536 ± 0.015
1.082TrpGly: 1.082 ± 0.026
0.37TrpHis: 0.37 ± 0.013
0.575TrpIle: 0.575 ± 0.014
0.389TrpLys: 0.389 ± 0.014
1.786TrpLeu: 1.786 ± 0.027
0.311TrpMet: 0.311 ± 0.011
0.445TrpAsn: 0.445 ± 0.016
0.794TrpPro: 0.794 ± 0.018
0.664TrpGln: 0.664 ± 0.017
1.334TrpArg: 1.334 ± 0.023
0.951TrpSer: 0.951 ± 0.021
1.089TrpThr: 1.089 ± 0.022
0.997TrpVal: 0.997 ± 0.022
0.358TrpTrp: 0.358 ± 0.012
0.377TrpTyr: 0.377 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.793TyrAla: 2.793 ± 0.035
0.18TyrCys: 0.18 ± 0.008
1.631TyrAsp: 1.631 ± 0.026
1.276TyrGlu: 1.276 ± 0.023
0.666TyrPhe: 0.666 ± 0.017
2.358TyrGly: 2.358 ± 0.034
0.384TyrHis: 0.384 ± 0.012
0.487TyrIle: 0.487 ± 0.016
0.447TyrLys: 0.447 ± 0.015
2.108TyrLeu: 2.108 ± 0.03
0.27TyrMet: 0.27 ± 0.011
0.436TyrAsn: 0.436 ± 0.015
1.06TyrPro: 1.06 ± 0.022
0.604TyrGln: 0.604 ± 0.017
1.846TyrArg: 1.846 ± 0.028
0.956TyrSer: 0.956 ± 0.018
1.24TyrThr: 1.24 ± 0.024
1.731TyrVal: 1.731 ± 0.026
0.369TyrTrp: 0.369 ± 0.012
0.453TyrTyr: 0.453 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7371 proteins (2399847 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski