Amino acid dipepetide frequency for Saccharopolyspora spinosa

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.162AlaAla: 19.162 ± 0.159	1.042AlaCys: 1.042 ± 0.025	8.303AlaAsp: 8.303 ± 0.079	10.077AlaGlu: 10.077 ± 0.159	3.316AlaPhe: 3.316 ± 0.045	12.373AlaGly: 12.373 ± 0.14	2.525AlaHis: 2.525 ± 0.033	4.173AlaIle: 4.173 ± 0.063	3.07AlaLys: 3.07 ± 0.057	12.84AlaLeu: 12.84 ± 0.122	2.504AlaMet: 2.504 ± 0.032	2.31AlaAsn: 2.31 ± 0.035	5.728AlaPro: 5.728 ± 0.067	4.194AlaGln: 4.194 ± 0.072	9.29AlaArg: 9.29 ± 0.069	5.702AlaSer: 5.702 ± 0.058	6.54AlaThr: 6.54 ± 0.068	11.678AlaVal: 11.678 ± 0.099	1.902AlaTrp: 1.902 ± 0.049	2.067AlaTyr: 2.067 ± 0.036	0.0AlaXaa: 0.0 ± 0.0
Cys	1.065CysAla: 1.065 ± 0.024	0.11CysCys: 0.11 ± 0.008	0.512CysAsp: 0.512 ± 0.017	0.435CysGlu: 0.435 ± 0.015	0.236CysPhe: 0.236 ± 0.01	0.979CysGly: 0.979 ± 0.027	0.204CysHis: 0.204 ± 0.01	0.215CysIle: 0.215 ± 0.011	0.125CysLys: 0.125 ± 0.007	0.742CysLeu: 0.742 ± 0.019	0.138CysMet: 0.138 ± 0.008	0.151CysAsn: 0.151 ± 0.008	0.514CysPro: 0.514 ± 0.016	0.235CysGln: 0.235 ± 0.011	0.683CysArg: 0.683 ± 0.018	0.518CysSer: 0.518 ± 0.017	0.496CysThr: 0.496 ± 0.015	0.683CysVal: 0.683 ± 0.019	0.147CysTrp: 0.147 ± 0.008	0.2CysTyr: 0.2 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.404AspAla: 7.404 ± 0.065	0.444AspCys: 0.444 ± 0.015	3.636AspAsp: 3.636 ± 0.046	4.28AspGlu: 4.28 ± 0.047	1.684AspPhe: 1.684 ± 0.027	5.895AspGly: 5.895 ± 0.073	1.383AspHis: 1.383 ± 0.026	1.932AspIle: 1.932 ± 0.037	1.046AspLys: 1.046 ± 0.026	6.206AspLeu: 6.206 ± 0.058	0.796AspMet: 0.796 ± 0.02	1.002AspAsn: 1.002 ± 0.025	4.168AspPro: 4.168 ± 0.048	2.005AspGln: 2.005 ± 0.03	5.093AspArg: 5.093 ± 0.062	2.746AspSer: 2.746 ± 0.047	2.678AspThr: 2.678 ± 0.036	5.218AspVal: 5.218 ± 0.056	0.993AspTrp: 0.993 ± 0.021	1.222AspTyr: 1.222 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.079GluAla: 7.079 ± 0.112	0.398GluCys: 0.398 ± 0.015	2.612GluAsp: 2.612 ± 0.04	3.355GluGlu: 3.355 ± 0.079	1.91GluPhe: 1.91 ± 0.03	3.859GluGly: 3.859 ± 0.088	1.783GluHis: 1.783 ± 0.031	2.664GluIle: 2.664 ± 0.043	1.465GluLys: 1.465 ± 0.037	8.627GluLeu: 8.627 ± 0.168	0.981GluMet: 0.981 ± 0.021	1.125GluAsn: 1.125 ± 0.024	3.49GluPro: 3.49 ± 0.049	3.141GluGln: 3.141 ± 0.092	5.428GluArg: 5.428 ± 0.063	3.057GluSer: 3.057 ± 0.052	2.875GluThr: 2.875 ± 0.04	5.219GluVal: 5.219 ± 0.055	0.887GluTrp: 0.887 ± 0.03	1.068GluTyr: 1.068 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	3.865PheAla: 3.865 ± 0.047	0.297PheCys: 0.297 ± 0.012	2.205PheAsp: 2.205 ± 0.035	1.578PheGlu: 1.578 ± 0.031	0.89PhePhe: 0.89 ± 0.023	3.428PheGly: 3.428 ± 0.045	0.647PheHis: 0.647 ± 0.017	0.747PheIle: 0.747 ± 0.023	0.429PheLys: 0.429 ± 0.015	2.546PheLeu: 2.546 ± 0.035	0.375PheMet: 0.375 ± 0.014	0.613PheAsn: 0.613 ± 0.018	1.416PhePro: 1.416 ± 0.026	0.74PheGln: 0.74 ± 0.017	1.913PheArg: 1.913 ± 0.032	1.576PheSer: 1.576 ± 0.023	1.761PheThr: 1.761 ± 0.034	2.467PheVal: 2.467 ± 0.037	0.398PheTrp: 0.398 ± 0.013	0.555PheTyr: 0.555 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	10.04GlyAla: 10.04 ± 0.09	0.874GlyCys: 0.874 ± 0.023	5.009GlyAsp: 5.009 ± 0.066	5.521GlyGlu: 5.521 ± 0.082	3.037GlyPhe: 3.037 ± 0.044	8.486GlyGly: 8.486 ± 0.115	2.108GlyHis: 2.108 ± 0.035	3.583GlyIle: 3.583 ± 0.047	2.574GlyLys: 2.574 ± 0.051	9.069GlyLeu: 9.069 ± 0.072	2.09GlyMet: 2.09 ± 0.033	1.888GlyAsn: 1.888 ± 0.032	4.733GlyPro: 4.733 ± 0.087	3.016GlyGln: 3.016 ± 0.043	6.961GlyArg: 6.961 ± 0.07	5.512GlySer: 5.512 ± 0.088	5.104GlyThr: 5.104 ± 0.051	8.088GlyVal: 8.088 ± 0.114	1.752GlyTrp: 1.752 ± 0.035	2.124GlyTyr: 2.124 ± 0.031	0.0GlyXaa: 0.0 ± 0.0
His	2.577HisAla: 2.577 ± 0.039	0.236HisCys: 0.236 ± 0.009	1.418HisAsp: 1.418 ± 0.027	1.223HisGlu: 1.223 ± 0.027	0.63HisPhe: 0.63 ± 0.017	2.254HisGly: 2.254 ± 0.035	0.651HisHis: 0.651 ± 0.019	0.676HisIle: 0.676 ± 0.018	0.316HisLys: 0.316 ± 0.013	2.359HisLeu: 2.359 ± 0.038	0.288HisMet: 0.288 ± 0.011	0.498HisAsn: 0.498 ± 0.016	1.703HisPro: 1.703 ± 0.029	0.774HisGln: 0.774 ± 0.019	2.132HisArg: 2.132 ± 0.037	1.065HisSer: 1.065 ± 0.023	1.135HisThr: 1.135 ± 0.025	1.787HisVal: 1.787 ± 0.028	0.381HisTrp: 0.381 ± 0.015	0.505HisTyr: 0.505 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	5.223IleAla: 5.223 ± 0.066	0.325IleCys: 0.325 ± 0.013	2.591IleAsp: 2.591 ± 0.041	2.278IleGlu: 2.278 ± 0.044	0.775IlePhe: 0.775 ± 0.023	3.874IleGly: 3.874 ± 0.062	0.623IleHis: 0.623 ± 0.018	1.067IleIle: 1.067 ± 0.029	0.788IleLys: 0.788 ± 0.016	2.414IleLeu: 2.414 ± 0.046	0.498IleMet: 0.498 ± 0.016	0.896IleAsn: 0.896 ± 0.021	2.043IlePro: 2.043 ± 0.036	0.796IleGln: 0.796 ± 0.019	2.591IleArg: 2.591 ± 0.042	2.057IleSer: 2.057 ± 0.039	2.4IleThr: 2.4 ± 0.049	2.963IleVal: 2.963 ± 0.042	0.412IleTrp: 0.412 ± 0.015	0.677IleTyr: 0.677 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	2.853LysAla: 2.853 ± 0.081	0.124LysCys: 0.124 ± 0.007	1.094LysAsp: 1.094 ± 0.024	1.245LysGlu: 1.245 ± 0.083	0.578LysPhe: 0.578 ± 0.02	1.489LysGly: 1.489 ± 0.037	0.567LysHis: 0.567 ± 0.014	0.964LysIle: 0.964 ± 0.027	0.882LysLys: 0.882 ± 0.053	2.23LysLeu: 2.23 ± 0.036	0.429LysMet: 0.429 ± 0.014	0.524LysAsn: 0.524 ± 0.02	1.467LysPro: 1.467 ± 0.032	1.113LysGln: 1.113 ± 0.06	1.813LysArg: 1.813 ± 0.048	1.188LysSer: 1.188 ± 0.024	1.192LysThr: 1.192 ± 0.022	1.994LysVal: 1.994 ± 0.047	0.345LysTrp: 0.345 ± 0.018	0.476LysTyr: 0.476 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	14.779LeuAla: 14.779 ± 0.104	0.845LeuCys: 0.845 ± 0.023	6.611LeuAsp: 6.611 ± 0.072	5.127LeuGlu: 5.127 ± 0.085	2.687LeuPhe: 2.687 ± 0.038	9.096LeuGly: 9.096 ± 0.083	2.329LeuHis: 2.329 ± 0.033	3.477LeuIle: 3.477 ± 0.058	1.858LeuLys: 1.858 ± 0.037	10.515LeuLeu: 10.515 ± 0.119	1.443LeuMet: 1.443 ± 0.028	1.853LeuAsn: 1.853 ± 0.031	6.209LeuPro: 6.209 ± 0.063	2.63LeuGln: 2.63 ± 0.041	8.979LeuArg: 8.979 ± 0.094	5.543LeuSer: 5.543 ± 0.059	6.012LeuThr: 6.012 ± 0.065	8.935LeuVal: 8.935 ± 0.107	1.215LeuTrp: 1.215 ± 0.03	1.558LeuTyr: 1.558 ± 0.028	0.0LeuXaa: 0.0 ± 0.0
Met	2.158MetAla: 2.158 ± 0.027	0.151MetCys: 0.151 ± 0.008	0.773MetAsp: 0.773 ± 0.02	0.632MetGlu: 0.632 ± 0.017	0.498MetPhe: 0.498 ± 0.015	1.143MetGly: 1.143 ± 0.021	0.382MetHis: 0.382 ± 0.014	0.74MetIle: 0.74 ± 0.019	0.351MetLys: 0.351 ± 0.014	1.918MetLeu: 1.918 ± 0.03	0.311MetMet: 0.311 ± 0.013	0.442MetAsn: 0.442 ± 0.015	1.165MetPro: 1.165 ± 0.023	0.549MetGln: 0.549 ± 0.017	1.529MetArg: 1.529 ± 0.027	1.433MetSer: 1.433 ± 0.028	1.495MetThr: 1.495 ± 0.031	1.311MetVal: 1.311 ± 0.026	0.264MetTrp: 0.264 ± 0.012	0.253MetTyr: 0.253 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.516AsnAla: 2.516 ± 0.038	0.236AsnCys: 0.236 ± 0.011	1.099AsnAsp: 1.099 ± 0.022	1.013AsnGlu: 1.013 ± 0.029	0.541AsnPhe: 0.541 ± 0.018	1.949AsnGly: 1.949 ± 0.034	0.434AsnHis: 0.434 ± 0.015	0.746AsnIle: 0.746 ± 0.023	0.413AsnLys: 0.413 ± 0.016	1.882AsnLeu: 1.882 ± 0.033	0.313AsnMet: 0.313 ± 0.013	0.494AsnAsn: 0.494 ± 0.018	1.584AsnPro: 1.584 ± 0.029	0.654AsnGln: 0.654 ± 0.02	1.566AsnArg: 1.566 ± 0.03	1.101AsnSer: 1.101 ± 0.024	1.115AsnThr: 1.115 ± 0.026	1.542AsnVal: 1.542 ± 0.027	0.351AsnTrp: 0.351 ± 0.013	0.477AsnTyr: 0.477 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	7.328ProAla: 7.328 ± 0.073	0.372ProCys: 0.372 ± 0.013	4.336ProAsp: 4.336 ± 0.058	4.275ProGlu: 4.275 ± 0.049	1.512ProPhe: 1.512 ± 0.029	5.951ProGly: 5.951 ± 0.072	1.233ProHis: 1.233 ± 0.028	1.713ProIle: 1.713 ± 0.035	1.553ProLys: 1.553 ± 0.058	5.062ProLeu: 5.062 ± 0.056	1.02ProMet: 1.02 ± 0.02	1.223ProAsn: 1.223 ± 0.026	3.434ProPro: 3.434 ± 0.059	1.942ProGln: 1.942 ± 0.033	3.544ProArg: 3.544 ± 0.051	3.074ProSer: 3.074 ± 0.041	3.202ProThr: 3.202 ± 0.045	5.345ProVal: 5.345 ± 0.054	0.828ProTrp: 0.828 ± 0.023	0.992ProTyr: 0.992 ± 0.021	0.0ProXaa: 0.0 ± 0.0
Gln	4.276GlnAla: 4.276 ± 0.065	0.216GlnCys: 0.216 ± 0.009	1.482GlnAsp: 1.482 ± 0.029	1.718GlnGlu: 1.718 ± 0.051	0.843GlnPhe: 0.843 ± 0.02	2.486GlnGly: 2.486 ± 0.059	0.852GlnHis: 0.852 ± 0.019	1.274GlnIle: 1.274 ± 0.025	0.996GlnLys: 0.996 ± 0.104	3.592GlnLeu: 3.592 ± 0.047	0.521GlnMet: 0.521 ± 0.016	0.634GlnAsn: 0.634 ± 0.022	2.215GlnPro: 2.215 ± 0.042	1.933GlnGln: 1.933 ± 0.058	3.241GlnArg: 3.241 ± 0.042	1.346GlnSer: 1.346 ± 0.027	1.4GlnThr: 1.4 ± 0.025	2.962GlnVal: 2.962 ± 0.035	0.667GlnTrp: 0.667 ± 0.032	0.589GlnTyr: 0.589 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	9.167ArgAla: 9.167 ± 0.082	0.742ArgCys: 0.742 ± 0.018	4.417ArgAsp: 4.417 ± 0.044	4.896ArgGlu: 4.896 ± 0.067	2.529ArgPhe: 2.529 ± 0.035	5.999ArgGly: 5.999 ± 0.075	1.906ArgHis: 1.906 ± 0.034	3.342ArgIle: 3.342 ± 0.06	2.178ArgLys: 2.178 ± 0.06	8.282ArgLeu: 8.282 ± 0.093	1.891ArgMet: 1.891 ± 0.033	1.698ArgAsn: 1.698 ± 0.029	4.519ArgPro: 4.519 ± 0.061	2.689ArgGln: 2.689 ± 0.045	7.702ArgArg: 7.702 ± 0.077	4.576ArgSer: 4.576 ± 0.051	4.483ArgThr: 4.483 ± 0.053	6.112ArgVal: 6.112 ± 0.068	1.48ArgTrp: 1.48 ± 0.027	1.867ArgTyr: 1.867 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	6.838SerAla: 6.838 ± 0.068	0.436SerCys: 0.436 ± 0.015	2.952SerAsp: 2.952 ± 0.047	2.815SerGlu: 2.815 ± 0.048	1.615SerPhe: 1.615 ± 0.029	6.281SerGly: 6.281 ± 0.098	1.025SerHis: 1.025 ± 0.02	1.797SerIle: 1.797 ± 0.035	1.162SerLys: 1.162 ± 0.025	4.725SerLeu: 4.725 ± 0.062	1.202SerMet: 1.202 ± 0.024	1.087SerAsn: 1.087 ± 0.023	3.045SerPro: 3.045 ± 0.044	1.527SerGln: 1.527 ± 0.032	3.919SerArg: 3.919 ± 0.039	3.174SerSer: 3.174 ± 0.051	3.207SerThr: 3.207 ± 0.046	4.436SerVal: 4.436 ± 0.042	0.981SerTrp: 0.981 ± 0.02	1.104SerTyr: 1.104 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	7.393ThrAla: 7.393 ± 0.08	0.465ThrCys: 0.465 ± 0.014	3.295ThrAsp: 3.295 ± 0.038	3.505ThrGlu: 3.505 ± 0.049	1.502ThrPhe: 1.502 ± 0.026	5.962ThrGly: 5.962 ± 0.064	1.116ThrHis: 1.116 ± 0.025	1.989ThrIle: 1.989 ± 0.04	1.199ThrLys: 1.199 ± 0.022	4.713ThrLeu: 4.713 ± 0.068	0.889ThrMet: 0.889 ± 0.02	1.12ThrAsn: 1.12 ± 0.026	3.502ThrPro: 3.502 ± 0.046	1.361ThrGln: 1.361 ± 0.027	3.708ThrArg: 3.708 ± 0.051	3.126ThrSer: 3.126 ± 0.042	3.618ThrThr: 3.618 ± 0.054	5.017ThrVal: 5.017 ± 0.054	0.845ThrTrp: 0.845 ± 0.023	1.107ThrTyr: 1.107 ± 0.021	0.0ThrXaa: 0.0 ± 0.0
Val	11.383ValAla: 11.383 ± 0.113	0.712ValCys: 0.712 ± 0.02	5.566ValAsp: 5.566 ± 0.05	5.311ValGlu: 5.311 ± 0.077	2.473ValPhe: 2.473 ± 0.038	7.156ValGly: 7.156 ± 0.083	1.992ValHis: 1.992 ± 0.033	3.116ValIle: 3.116 ± 0.041	1.505ValLys: 1.505 ± 0.025	9.888ValLeu: 9.888 ± 0.092	1.273ValMet: 1.273 ± 0.02	1.718ValAsn: 1.718 ± 0.03	5.094ValPro: 5.094 ± 0.058	2.434ValGln: 2.434 ± 0.035	6.958ValArg: 6.958 ± 0.07	4.561ValSer: 4.561 ± 0.048	4.818ValThr: 4.818 ± 0.053	8.773ValVal: 8.773 ± 0.101	1.141ValTrp: 1.141 ± 0.026	1.313ValTyr: 1.313 ± 0.024	0.0ValXaa: 0.0 ± 0.0
Trp	1.566TrpAla: 1.566 ± 0.026	0.173TrpCys: 0.173 ± 0.009	0.766TrpAsp: 0.766 ± 0.022	0.707TrpGlu: 0.707 ± 0.021	0.536TrpPhe: 0.536 ± 0.016	1.095TrpGly: 1.095 ± 0.027	0.403TrpHis: 0.403 ± 0.014	0.605TrpIle: 0.605 ± 0.017	0.349TrpLys: 0.349 ± 0.014	1.958TrpLeu: 1.958 ± 0.034	0.335TrpMet: 0.335 ± 0.013	0.371TrpAsn: 0.371 ± 0.013	0.839TrpPro: 0.839 ± 0.019	0.734TrpGln: 0.734 ± 0.027	1.488TrpArg: 1.488 ± 0.036	0.985TrpSer: 0.985 ± 0.022	0.945TrpThr: 0.945 ± 0.022	1.163TrpVal: 1.163 ± 0.037	0.381TrpTrp: 0.381 ± 0.016	0.33TrpTyr: 0.33 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.125TyrAla: 2.125 ± 0.035	0.191TyrCys: 0.191 ± 0.009	1.151TyrAsp: 1.151 ± 0.026	1.033TyrGlu: 1.033 ± 0.025	0.652TyrPhe: 0.652 ± 0.018	1.814TyrGly: 1.814 ± 0.034	0.447TyrHis: 0.447 ± 0.015	0.474TyrIle: 0.474 ± 0.014	0.311TyrLys: 0.311 ± 0.012	2.133TyrLeu: 2.133 ± 0.035	0.2TyrMet: 0.2 ± 0.009	0.379TyrAsn: 0.379 ± 0.012	1.073TyrPro: 1.073 ± 0.026	0.776TyrGln: 0.776 ± 0.026	1.937TyrArg: 1.937 ± 0.033	0.984TyrSer: 0.984 ± 0.022	0.954TyrThr: 0.954 ± 0.02	1.493TyrVal: 1.493 ± 0.027	0.358TyrTrp: 0.358 ± 0.013	0.435TyrTyr: 0.435 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7300 proteins (2427316 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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