Tepidibacillus fermentans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Tepidibacillus

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2415 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R3KKL0|A0A4R3KKL0_9BACI Transcriptional regulator MraZ OS=Tepidibacillus fermentans OX=1281767 GN=mraZ PE=3 SV=1
MM1 pKa = 7.24NRR3 pKa = 11.84IQEE6 pKa = 4.47RR7 pKa = 11.84ISYY10 pKa = 10.55LEE12 pKa = 3.98GLAEE16 pKa = 5.27GLDD19 pKa = 3.74LYY21 pKa = 11.26KK22 pKa = 10.87NKK24 pKa = 10.22PEE26 pKa = 4.12GKK28 pKa = 9.89FLEE31 pKa = 4.32EE32 pKa = 3.5LMAVIKK38 pKa = 9.94EE39 pKa = 4.16LNEE42 pKa = 4.18TLQHH46 pKa = 4.79TTNRR50 pKa = 11.84LAEE53 pKa = 4.12LEE55 pKa = 4.39EE56 pKa = 4.15YY57 pKa = 11.2VEE59 pKa = 5.95VIDD62 pKa = 5.57EE63 pKa = 4.58DD64 pKa = 5.03LNDD67 pKa = 5.07LEE69 pKa = 4.05VDD71 pKa = 4.1YY72 pKa = 11.55YY73 pKa = 11.55NEE75 pKa = 4.69PDD77 pKa = 4.26FDD79 pKa = 7.4DD80 pKa = 5.75IDD82 pKa = 6.13DD83 pKa = 4.63EE84 pKa = 5.75DD85 pKa = 6.11DD86 pKa = 4.72EE87 pKa = 6.61IDD89 pKa = 5.44DD90 pKa = 4.68EE91 pKa = 5.14EE92 pKa = 6.01IDD94 pKa = 5.49DD95 pKa = 4.94GIQYY99 pKa = 10.42FEE101 pKa = 4.53VEE103 pKa = 4.53CPSCHH108 pKa = 5.82EE109 pKa = 5.17LIMVDD114 pKa = 3.04QDD116 pKa = 3.47FFEE119 pKa = 6.61GEE121 pKa = 4.3GPTDD125 pKa = 3.73IVCPNCDD132 pKa = 3.15HH133 pKa = 7.21VFILNDD139 pKa = 3.47EE140 pKa = 4.57DD141 pKa = 4.91SVTNVKK147 pKa = 10.28

Molecular weight: 17.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3KKP9|A0A4R3KKP9_9BACI N-acetylmuramoyl-L-alanine amidase OS=Tepidibacillus fermentans OX=1281767 GN=EDD72_101129 PE=4 SV=1
MM1 pKa = 7.27GRR3 pKa = 11.84LAQGMITGGIVAAVIGIVMRR23 pKa = 11.84RR24 pKa = 11.84SRR26 pKa = 11.84HH27 pKa = 4.17NQKK30 pKa = 9.1MLNRR34 pKa = 11.84YY35 pKa = 8.36MNMLINMMAKK45 pKa = 9.84NGMFRR50 pKa = 11.84LIGNSRR56 pKa = 11.84FFRR59 pKa = 11.84NMVRR63 pKa = 11.84ARR65 pKa = 3.45

Molecular weight: 7.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2415

0

2415

702271

29

2402

290.8

32.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.361 ± 0.055

0.501 ± 0.015

4.897 ± 0.033

7.454 ± 0.056

4.34 ± 0.041

6.824 ± 0.049

2.123 ± 0.023

8.929 ± 0.051

7.448 ± 0.041

9.782 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.724 ± 0.021

4.358 ± 0.035

3.681 ± 0.031

4.32 ± 0.046

4.306 ± 0.032

5.399 ± 0.034

5.179 ± 0.031

6.83 ± 0.043

0.94 ± 0.018

3.606 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski