Cellulosimicrobium sp. CUA-896

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Promicromonosporaceae; Cellulosimicrobium; unclassified Cellulosimicrobium

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2595 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9VTY0|A0A1Q9VTY0_9MICO 4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cellulosimicrobium sp. CUA-896 OX=1517881 GN=ispH PE=3 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84NRR4 pKa = 11.84RR5 pKa = 11.84FAALTALGLSGVLALTACSSGSDD28 pKa = 4.77DD29 pKa = 4.47DD30 pKa = 5.34AQDD33 pKa = 3.62ASSSGSSTGVVTVSNGEE50 pKa = 4.02PQNPLIPTMTNEE62 pKa = 4.47TNGSLTVQNIFSGLVYY78 pKa = 10.46YY79 pKa = 10.48DD80 pKa = 3.87AEE82 pKa = 4.79GGVHH86 pKa = 6.27NEE88 pKa = 3.69VAEE91 pKa = 4.56SIEE94 pKa = 4.88SEE96 pKa = 4.88DD97 pKa = 3.58NQAWTITIADD107 pKa = 3.62GWTFTDD113 pKa = 4.29GTPVTAEE120 pKa = 4.16SFASAWDD127 pKa = 3.45YY128 pKa = 11.6GAALDD133 pKa = 3.96NAQNLSYY140 pKa = 10.31FFEE143 pKa = 5.52PIQGFSYY150 pKa = 10.79DD151 pKa = 3.88ANTSLIEE158 pKa = 4.06EE159 pKa = 4.76GGLVVEE165 pKa = 5.21DD166 pKa = 4.6EE167 pKa = 4.5STLVVNLTQPEE178 pKa = 4.24SDD180 pKa = 3.48FPIRR184 pKa = 11.84LGYY187 pKa = 10.46AAFFPLPEE195 pKa = 4.7AFFDD199 pKa = 3.98DD200 pKa = 4.42PEE202 pKa = 5.75AFGEE206 pKa = 4.29APVGNGPYY214 pKa = 9.51MLEE217 pKa = 3.98TWEE220 pKa = 4.41HH221 pKa = 6.9DD222 pKa = 3.68SLISLVPNPDD232 pKa = 3.2YY233 pKa = 11.26AGEE236 pKa = 4.0RR237 pKa = 11.84TPANGGLEE245 pKa = 4.4LIAYY249 pKa = 7.76TDD251 pKa = 3.52EE252 pKa = 4.53DD253 pKa = 3.65SAYY256 pKa = 10.87NDD258 pKa = 3.97LLGGQLDD265 pKa = 4.02IIKK268 pKa = 9.85NVPSSAFATFEE279 pKa = 4.38DD280 pKa = 4.58EE281 pKa = 4.79LEE283 pKa = 4.34GRR285 pKa = 11.84AVNQPAAVIQTLTVPEE301 pKa = 4.52WLPEE305 pKa = 4.09FQGEE309 pKa = 4.11AGLLRR314 pKa = 11.84RR315 pKa = 11.84QAISHH320 pKa = 6.88AIDD323 pKa = 3.42RR324 pKa = 11.84DD325 pKa = 3.9EE326 pKa = 4.0VTEE329 pKa = 4.48TIFAGSRR336 pKa = 11.84TPATDD341 pKa = 3.39FTSPVIDD348 pKa = 3.43GWTEE352 pKa = 3.93EE353 pKa = 4.07IPGNEE358 pKa = 3.94VLEE361 pKa = 4.18YY362 pKa = 10.84DD363 pKa = 3.82ADD365 pKa = 4.0LAAEE369 pKa = 4.42LWGEE373 pKa = 4.16AEE375 pKa = 4.59AIEE378 pKa = 4.26PVGDD382 pKa = 3.55YY383 pKa = 9.92TLQIASNADD392 pKa = 3.24SDD394 pKa = 3.84HH395 pKa = 5.38QTWVDD400 pKa = 3.57AVCNNIRR407 pKa = 11.84TVLEE411 pKa = 3.96IGCEE415 pKa = 3.95FFPYY419 pKa = 8.83PTFDD423 pKa = 3.43EE424 pKa = 4.44YY425 pKa = 11.77LDD427 pKa = 4.11ARR429 pKa = 11.84DD430 pKa = 3.95SGSVPGPFRR439 pKa = 11.84GGWQADD445 pKa = 3.86YY446 pKa = 9.61PAMSNFLGPIYY457 pKa = 9.96GTGAGSNDD465 pKa = 4.04GQWSNADD472 pKa = 3.07FDD474 pKa = 4.24ATLTEE479 pKa = 4.47ANSAEE484 pKa = 4.15TRR486 pKa = 11.84SRR488 pKa = 11.84RR489 pKa = 11.84SSCTWTPRR497 pKa = 11.84RR498 pKa = 11.84SCSRR502 pKa = 11.84TCRR505 pKa = 11.84ALPLWYY511 pKa = 10.69SNATGGWADD520 pKa = 3.41TVEE523 pKa = 4.0NVEE526 pKa = 4.84FGWDD530 pKa = 3.68SDD532 pKa = 4.44PILYY536 pKa = 10.08QVTKK540 pKa = 11.13AEE542 pKa = 4.09

Molecular weight: 58.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9VWY3|A0A1Q9VWY3_9MICO Helicase ATP-binding domain-containing protein OS=Cellulosimicrobium sp. CUA-896 OX=1517881 GN=BJF88_09305 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPNTRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.83THH17 pKa = 5.18GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.97GRR40 pKa = 11.84TEE42 pKa = 3.99LSAA45 pKa = 4.86

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2595

0

2595

732398

37

1957

282.2

29.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.639 ± 0.079

0.623 ± 0.013

6.596 ± 0.052

5.442 ± 0.051

2.396 ± 0.032

9.608 ± 0.049

2.009 ± 0.025

2.403 ± 0.037

1.19 ± 0.027

9.852 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.471 ± 0.019

1.332 ± 0.026

6.193 ± 0.047

2.395 ± 0.028

8.453 ± 0.069

5.214 ± 0.045

6.456 ± 0.048

10.491 ± 0.055

1.514 ± 0.021

1.723 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski