Amino acid dipepetide frequency for Enterococcus phage vB_EfaS_HEf13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.145AlaAla: 6.145 ± 1.452
0.537AlaCys: 0.537 ± 0.226
4.594AlaAsp: 4.594 ± 0.609
6.264AlaGlu: 6.264 ± 0.633
3.102AlaPhe: 3.102 ± 0.402
4.713AlaGly: 4.713 ± 0.748
0.776AlaHis: 0.776 ± 0.261
5.548AlaIle: 5.548 ± 0.593
4.713AlaLys: 4.713 ± 0.781
5.847AlaLeu: 5.847 ± 0.667
2.386AlaMet: 2.386 ± 0.404
3.52AlaAsn: 3.52 ± 0.444
2.028AlaPro: 2.028 ± 0.356
2.028AlaGln: 2.028 ± 0.401
3.52AlaArg: 3.52 ± 0.402
4.236AlaSer: 4.236 ± 0.502
4.176AlaThr: 4.176 ± 0.603
4.474AlaVal: 4.474 ± 0.547
0.537AlaTrp: 0.537 ± 0.172
3.222AlaTyr: 3.222 ± 0.55
0.0AlaXaa: 0.0 ± 0.0
Cys
0.358CysAla: 0.358 ± 0.151
0.239CysCys: 0.239 ± 0.139
0.418CysAsp: 0.418 ± 0.136
0.716CysGlu: 0.716 ± 0.184
0.477CysPhe: 0.477 ± 0.173
0.895CysGly: 0.895 ± 0.276
0.06CysHis: 0.06 ± 0.054
0.656CysIle: 0.656 ± 0.245
0.477CysLys: 0.477 ± 0.25
0.776CysLeu: 0.776 ± 0.229
0.239CysMet: 0.239 ± 0.117
0.119CysAsn: 0.119 ± 0.096
0.06CysPro: 0.06 ± 0.057
0.358CysGln: 0.358 ± 0.152
0.179CysArg: 0.179 ± 0.114
0.358CysSer: 0.358 ± 0.134
0.119CysThr: 0.119 ± 0.08
0.239CysVal: 0.239 ± 0.125
0.06CysTrp: 0.06 ± 0.057
0.179CysTyr: 0.179 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
4.355AspAla: 4.355 ± 0.477
0.537AspCys: 0.537 ± 0.185
3.222AspAsp: 3.222 ± 0.61
4.653AspGlu: 4.653 ± 0.461
3.997AspPhe: 3.997 ± 0.394
3.699AspGly: 3.699 ± 0.617
0.477AspHis: 0.477 ± 0.182
4.236AspIle: 4.236 ± 0.563
4.474AspLys: 4.474 ± 0.476
5.489AspLeu: 5.489 ± 0.559
2.148AspMet: 2.148 ± 0.451
2.804AspAsn: 2.804 ± 0.402
1.432AspPro: 1.432 ± 0.274
1.193AspGln: 1.193 ± 0.292
2.148AspArg: 2.148 ± 0.374
3.937AspSer: 3.937 ± 0.433
4.534AspThr: 4.534 ± 0.785
4.474AspVal: 4.474 ± 0.57
1.193AspTrp: 1.193 ± 0.369
4.116AspTyr: 4.116 ± 0.437
0.0AspXaa: 0.0 ± 0.0
Glu
8.412GluAla: 8.412 ± 0.821
0.597GluCys: 0.597 ± 0.188
6.026GluAsp: 6.026 ± 0.642
11.276GluGlu: 11.276 ± 1.327
3.341GluPhe: 3.341 ± 0.603
6.264GluGly: 6.264 ± 0.662
1.074GluHis: 1.074 ± 0.265
4.832GluIle: 4.832 ± 0.61
4.952GluLys: 4.952 ± 0.711
8.054GluLeu: 8.054 ± 0.933
2.744GluMet: 2.744 ± 0.421
4.474GluAsn: 4.474 ± 0.617
1.73GluPro: 1.73 ± 0.332
3.222GluGln: 3.222 ± 0.448
4.653GluArg: 4.653 ± 0.487
5.489GluSer: 5.489 ± 0.725
3.759GluThr: 3.759 ± 0.485
6.205GluVal: 6.205 ± 0.551
1.312GluTrp: 1.312 ± 0.307
3.401GluTyr: 3.401 ± 0.489
0.0GluXaa: 0.0 ± 0.0
Phe
2.804PheAla: 2.804 ± 0.4
0.298PheCys: 0.298 ± 0.128
2.923PheAsp: 2.923 ± 0.41
3.639PheGlu: 3.639 ± 0.44
1.432PhePhe: 1.432 ± 0.283
3.222PheGly: 3.222 ± 0.475
0.716PheHis: 0.716 ± 0.203
2.864PheIle: 2.864 ± 0.471
3.639PheLys: 3.639 ± 0.346
3.401PheLeu: 3.401 ± 0.415
1.014PheMet: 1.014 ± 0.259
2.327PheAsn: 2.327 ± 0.366
1.253PhePro: 1.253 ± 0.305
1.134PheGln: 1.134 ± 0.269
1.551PheArg: 1.551 ± 0.274
2.804PheSer: 2.804 ± 0.499
2.923PheThr: 2.923 ± 0.423
2.446PheVal: 2.446 ± 0.375
0.537PheTrp: 0.537 ± 0.191
1.67PheTyr: 1.67 ± 0.456
0.0PheXaa: 0.0 ± 0.0
Gly
3.52GlyAla: 3.52 ± 0.484
0.418GlyCys: 0.418 ± 0.146
3.222GlyAsp: 3.222 ± 0.551
4.534GlyGlu: 4.534 ± 0.48
3.341GlyPhe: 3.341 ± 0.483
3.937GlyGly: 3.937 ± 0.829
1.193GlyHis: 1.193 ± 0.268
4.653GlyIle: 4.653 ± 0.65
5.727GlyLys: 5.727 ± 0.772
5.011GlyLeu: 5.011 ± 0.588
1.67GlyMet: 1.67 ± 0.248
3.102GlyAsn: 3.102 ± 0.399
0.119GlyPro: 0.119 ± 0.083
1.909GlyGln: 1.909 ± 0.395
2.565GlyArg: 2.565 ± 0.355
3.52GlySer: 3.52 ± 0.374
4.474GlyThr: 4.474 ± 0.733
3.937GlyVal: 3.937 ± 0.587
1.014GlyTrp: 1.014 ± 0.302
3.043GlyTyr: 3.043 ± 0.407
0.0GlyXaa: 0.0 ± 0.0
His
1.014HisAla: 1.014 ± 0.273
0.179HisCys: 0.179 ± 0.101
0.597HisAsp: 0.597 ± 0.236
1.253HisGlu: 1.253 ± 0.269
1.014HisPhe: 1.014 ± 0.259
0.895HisGly: 0.895 ± 0.23
0.358HisHis: 0.358 ± 0.163
1.312HisIle: 1.312 ± 0.279
1.014HisLys: 1.014 ± 0.228
1.193HisLeu: 1.193 ± 0.302
0.298HisMet: 0.298 ± 0.137
0.895HisAsn: 0.895 ± 0.183
0.656HisPro: 0.656 ± 0.226
0.477HisGln: 0.477 ± 0.186
0.776HisArg: 0.776 ± 0.206
0.716HisSer: 0.716 ± 0.237
0.358HisThr: 0.358 ± 0.171
0.835HisVal: 0.835 ± 0.187
0.06HisTrp: 0.06 ± 0.061
0.656HisTyr: 0.656 ± 0.207
0.0HisXaa: 0.0 ± 0.0
Ile
5.071IleAla: 5.071 ± 0.591
0.477IleCys: 0.477 ± 0.174
4.653IleAsp: 4.653 ± 0.484
5.19IleGlu: 5.19 ± 0.596
2.207IlePhe: 2.207 ± 0.373
3.341IleGly: 3.341 ± 0.397
1.312IleHis: 1.312 ± 0.258
3.222IleIle: 3.222 ± 0.48
5.429IleLys: 5.429 ± 0.629
3.937IleLeu: 3.937 ± 0.613
1.611IleMet: 1.611 ± 0.262
4.176IleAsn: 4.176 ± 0.455
1.909IlePro: 1.909 ± 0.282
2.565IleGln: 2.565 ± 0.466
2.983IleArg: 2.983 ± 0.452
3.46IleSer: 3.46 ± 0.461
3.997IleThr: 3.997 ± 0.675
3.699IleVal: 3.699 ± 0.449
0.537IleTrp: 0.537 ± 0.193
2.864IleTyr: 2.864 ± 0.426
0.0IleXaa: 0.0 ± 0.0
Lys
7.099LysAla: 7.099 ± 0.67
0.358LysCys: 0.358 ± 0.153
5.608LysAsp: 5.608 ± 0.441
7.338LysGlu: 7.338 ± 0.967
3.102LysPhe: 3.102 ± 0.408
4.176LysGly: 4.176 ± 0.532
1.253LysHis: 1.253 ± 0.33
3.759LysIle: 3.759 ± 0.402
5.847LysLys: 5.847 ± 0.795
7.398LysLeu: 7.398 ± 0.659
2.386LysMet: 2.386 ± 0.471
2.983LysAsn: 2.983 ± 0.531
3.102LysPro: 3.102 ± 0.406
2.744LysGln: 2.744 ± 0.446
3.281LysArg: 3.281 ± 0.593
4.176LysSer: 4.176 ± 0.587
4.236LysThr: 4.236 ± 0.515
5.489LysVal: 5.489 ± 0.431
1.014LysTrp: 1.014 ± 0.225
2.446LysTyr: 2.446 ± 0.46
0.0LysXaa: 0.0 ± 0.0
Leu
5.906LeuAla: 5.906 ± 0.609
0.597LeuCys: 0.597 ± 0.207
5.19LeuAsp: 5.19 ± 0.383
9.665LeuGlu: 9.665 ± 1.099
2.864LeuPhe: 2.864 ± 0.446
5.071LeuGly: 5.071 ± 0.677
1.312LeuHis: 1.312 ± 0.3
5.429LeuIle: 5.429 ± 0.66
6.682LeuLys: 6.682 ± 0.754
6.383LeuLeu: 6.383 ± 0.883
2.565LeuMet: 2.565 ± 0.367
4.295LeuAsn: 4.295 ± 0.57
2.804LeuPro: 2.804 ± 0.342
3.58LeuGln: 3.58 ± 0.519
2.923LeuArg: 2.923 ± 0.399
5.369LeuSer: 5.369 ± 0.524
6.801LeuThr: 6.801 ± 0.68
5.429LeuVal: 5.429 ± 0.666
0.776LeuTrp: 0.776 ± 0.194
2.327LeuTyr: 2.327 ± 0.353
0.0LeuXaa: 0.0 ± 0.0
Met
2.685MetAla: 2.685 ± 0.493
0.119MetCys: 0.119 ± 0.076
1.73MetAsp: 1.73 ± 0.314
2.685MetGlu: 2.685 ± 0.444
1.074MetPhe: 1.074 ± 0.282
1.074MetGly: 1.074 ± 0.299
0.298MetHis: 0.298 ± 0.118
1.491MetIle: 1.491 ± 0.3
2.207MetLys: 2.207 ± 0.353
2.864MetLeu: 2.864 ± 0.428
0.477MetMet: 0.477 ± 0.164
1.73MetAsn: 1.73 ± 0.251
1.074MetPro: 1.074 ± 0.226
0.716MetGln: 0.716 ± 0.177
1.253MetArg: 1.253 ± 0.275
1.79MetSer: 1.79 ± 0.33
1.014MetThr: 1.014 ± 0.267
1.432MetVal: 1.432 ± 0.3
0.298MetTrp: 0.298 ± 0.106
0.835MetTyr: 0.835 ± 0.175
0.0MetXaa: 0.0 ± 0.0
Asn
3.102AsnAla: 3.102 ± 0.486
0.179AsnCys: 0.179 ± 0.105
2.923AsnAsp: 2.923 ± 0.422
4.594AsnGlu: 4.594 ± 0.522
2.148AsnPhe: 2.148 ± 0.316
3.699AsnGly: 3.699 ± 0.447
0.895AsnHis: 0.895 ± 0.223
3.281AsnIle: 3.281 ± 0.439
4.116AsnLys: 4.116 ± 0.546
3.997AsnLeu: 3.997 ± 0.509
0.955AsnMet: 0.955 ± 0.197
1.969AsnAsn: 1.969 ± 0.4
2.565AsnPro: 2.565 ± 0.568
1.969AsnGln: 1.969 ± 0.414
2.148AsnArg: 2.148 ± 0.318
3.222AsnSer: 3.222 ± 0.393
2.923AsnThr: 2.923 ± 0.416
3.281AsnVal: 3.281 ± 0.491
0.597AsnTrp: 0.597 ± 0.208
1.67AsnTyr: 1.67 ± 0.332
0.0AsnXaa: 0.0 ± 0.0
Pro
1.611ProAla: 1.611 ± 0.393
0.179ProCys: 0.179 ± 0.094
1.909ProAsp: 1.909 ± 0.369
3.222ProGlu: 3.222 ± 0.468
1.432ProPhe: 1.432 ± 0.318
0.179ProGly: 0.179 ± 0.084
0.418ProHis: 0.418 ± 0.149
2.028ProIle: 2.028 ± 0.321
2.804ProLys: 2.804 ± 0.393
2.088ProLeu: 2.088 ± 0.371
0.776ProMet: 0.776 ± 0.223
1.79ProAsn: 1.79 ± 0.387
0.835ProPro: 0.835 ± 0.226
0.597ProGln: 0.597 ± 0.209
1.193ProArg: 1.193 ± 0.295
2.744ProSer: 2.744 ± 0.445
1.67ProThr: 1.67 ± 0.292
1.79ProVal: 1.79 ± 0.347
0.477ProTrp: 0.477 ± 0.177
1.849ProTyr: 1.849 ± 0.405
0.0ProXaa: 0.0 ± 0.0
Gln
2.446GlnAla: 2.446 ± 0.403
0.179GlnCys: 0.179 ± 0.093
2.028GlnAsp: 2.028 ± 0.332
3.222GlnGlu: 3.222 ± 0.428
1.193GlnPhe: 1.193 ± 0.326
2.506GlnGly: 2.506 ± 0.416
0.537GlnHis: 0.537 ± 0.173
1.73GlnIle: 1.73 ± 0.407
2.327GlnLys: 2.327 ± 0.4
3.699GlnLeu: 3.699 ± 0.462
0.835GlnMet: 0.835 ± 0.253
1.312GlnAsn: 1.312 ± 0.291
1.014GlnPro: 1.014 ± 0.226
1.551GlnGln: 1.551 ± 0.461
1.79GlnArg: 1.79 ± 0.323
1.909GlnSer: 1.909 ± 0.365
2.506GlnThr: 2.506 ± 0.434
2.386GlnVal: 2.386 ± 0.363
0.418GlnTrp: 0.418 ± 0.156
1.372GlnTyr: 1.372 ± 0.321
0.0GlnXaa: 0.0 ± 0.0
Arg
2.506ArgAla: 2.506 ± 0.454
0.418ArgCys: 0.418 ± 0.161
2.327ArgAsp: 2.327 ± 0.348
3.162ArgGlu: 3.162 ± 0.329
2.148ArgPhe: 2.148 ± 0.328
2.267ArgGly: 2.267 ± 0.432
0.716ArgHis: 0.716 ± 0.222
3.281ArgIle: 3.281 ± 0.496
3.102ArgLys: 3.102 ± 0.463
4.295ArgLeu: 4.295 ± 0.534
0.656ArgMet: 0.656 ± 0.155
1.611ArgAsn: 1.611 ± 0.302
1.611ArgPro: 1.611 ± 0.337
1.611ArgGln: 1.611 ± 0.286
1.432ArgArg: 1.432 ± 0.358
1.909ArgSer: 1.909 ± 0.4
1.969ArgThr: 1.969 ± 0.329
2.744ArgVal: 2.744 ± 0.411
0.477ArgTrp: 0.477 ± 0.17
2.028ArgTyr: 2.028 ± 0.307
0.0ArgXaa: 0.0 ± 0.0
Ser
3.281SerAla: 3.281 ± 0.429
0.358SerCys: 0.358 ± 0.141
3.818SerAsp: 3.818 ± 0.448
4.355SerGlu: 4.355 ± 0.512
2.625SerPhe: 2.625 ± 0.31
3.937SerGly: 3.937 ± 0.431
0.716SerHis: 0.716 ± 0.198
3.818SerIle: 3.818 ± 0.486
5.906SerLys: 5.906 ± 0.483
5.369SerLeu: 5.369 ± 0.599
1.611SerMet: 1.611 ± 0.317
3.58SerAsn: 3.58 ± 0.429
1.67SerPro: 1.67 ± 0.246
2.028SerGln: 2.028 ± 0.404
1.909SerArg: 1.909 ± 0.362
2.864SerSer: 2.864 ± 0.33
3.878SerThr: 3.878 ± 0.54
3.759SerVal: 3.759 ± 0.465
1.134SerTrp: 1.134 ± 0.241
2.446SerTyr: 2.446 ± 0.401
0.0SerXaa: 0.0 ± 0.0
Thr
4.653ThrAla: 4.653 ± 0.839
0.298ThrCys: 0.298 ± 0.123
3.878ThrAsp: 3.878 ± 0.462
4.594ThrGlu: 4.594 ± 0.477
2.506ThrPhe: 2.506 ± 0.375
3.639ThrGly: 3.639 ± 0.63
0.895ThrHis: 0.895 ± 0.247
3.997ThrIle: 3.997 ± 0.515
3.878ThrLys: 3.878 ± 0.37
6.503ThrLeu: 6.503 ± 0.622
1.372ThrMet: 1.372 ± 0.318
2.804ThrAsn: 2.804 ± 0.534
2.446ThrPro: 2.446 ± 0.363
1.67ThrGln: 1.67 ± 0.358
1.432ThrArg: 1.432 ± 0.358
3.46ThrSer: 3.46 ± 0.577
3.043ThrThr: 3.043 ± 0.509
5.25ThrVal: 5.25 ± 0.704
0.656ThrTrp: 0.656 ± 0.236
3.043ThrTyr: 3.043 ± 0.452
0.0ThrXaa: 0.0 ± 0.0
Val
4.415ValAla: 4.415 ± 0.645
0.776ValCys: 0.776 ± 0.25
4.415ValAsp: 4.415 ± 0.52
5.966ValGlu: 5.966 ± 0.627
2.804ValPhe: 2.804 ± 0.413
3.639ValGly: 3.639 ± 0.531
0.776ValHis: 0.776 ± 0.199
3.759ValIle: 3.759 ± 0.427
6.085ValLys: 6.085 ± 0.622
5.071ValLeu: 5.071 ± 0.479
1.312ValMet: 1.312 ± 0.311
3.341ValAsn: 3.341 ± 0.392
2.207ValPro: 2.207 ± 0.422
2.923ValGln: 2.923 ± 0.355
2.625ValArg: 2.625 ± 0.418
3.341ValSer: 3.341 ± 0.518
3.937ValThr: 3.937 ± 0.602
3.878ValVal: 3.878 ± 0.591
1.432ValTrp: 1.432 ± 0.329
2.148ValTyr: 2.148 ± 0.421
0.0ValXaa: 0.0 ± 0.0
Trp
0.656TrpAla: 0.656 ± 0.179
0.119TrpCys: 0.119 ± 0.079
0.656TrpAsp: 0.656 ± 0.201
1.432TrpGlu: 1.432 ± 0.312
0.597TrpPhe: 0.597 ± 0.163
1.074TrpGly: 1.074 ± 0.334
0.239TrpHis: 0.239 ± 0.105
1.134TrpIle: 1.134 ± 0.238
0.716TrpLys: 0.716 ± 0.183
0.716TrpLeu: 0.716 ± 0.25
0.239TrpMet: 0.239 ± 0.114
1.074TrpAsn: 1.074 ± 0.208
0.119TrpPro: 0.119 ± 0.082
0.716TrpGln: 0.716 ± 0.255
0.179TrpArg: 0.179 ± 0.101
1.014TrpSer: 1.014 ± 0.303
1.074TrpThr: 1.074 ± 0.34
0.597TrpVal: 0.597 ± 0.164
0.119TrpTrp: 0.119 ± 0.083
0.656TrpTyr: 0.656 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.386TyrAla: 2.386 ± 0.384
0.119TyrCys: 0.119 ± 0.091
2.983TyrAsp: 2.983 ± 0.397
4.057TyrGlu: 4.057 ± 0.653
1.193TyrPhe: 1.193 ± 0.272
2.685TyrGly: 2.685 ± 0.422
0.537TyrHis: 0.537 ± 0.176
1.611TyrIle: 1.611 ± 0.267
3.699TyrLys: 3.699 ± 0.386
3.878TyrLeu: 3.878 ± 0.473
1.491TyrMet: 1.491 ± 0.316
2.327TyrAsn: 2.327 ± 0.392
0.955TyrPro: 0.955 ± 0.24
1.969TyrGln: 1.969 ± 0.278
1.73TyrArg: 1.73 ± 0.331
2.744TyrSer: 2.744 ± 0.381
2.625TyrThr: 2.625 ± 0.439
2.506TyrVal: 2.506 ± 0.288
0.477TyrTrp: 0.477 ± 0.161
2.327TyrTyr: 2.327 ± 0.452
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (16763 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski