Tortoise microvirus 35

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1FVX9|A0A4V1FVX9_9VIRU Uncharacterized protein OS=Tortoise microvirus 35 OX=2583138 PE=4 SV=1
MM1 pKa = 7.66SKK3 pKa = 10.6FLTQYY8 pKa = 11.02NYY10 pKa = 11.13LEE12 pKa = 4.85NIVDD16 pKa = 4.15SSVSRR21 pKa = 11.84YY22 pKa = 10.05DD23 pKa = 3.47YY24 pKa = 11.21SSYY27 pKa = 11.56SPLPSLTVPDD37 pKa = 3.94EE38 pKa = 4.21SLTVSEE44 pKa = 5.0LLDD47 pKa = 4.24RR48 pKa = 11.84YY49 pKa = 11.02AKK51 pKa = 9.84GQPLGVPYY59 pKa = 10.27YY60 pKa = 10.24EE61 pKa = 4.47PRR63 pKa = 11.84YY64 pKa = 9.25PDD66 pKa = 3.76NEE68 pKa = 4.26PDD70 pKa = 4.81FDD72 pKa = 5.51DD73 pKa = 5.51FDD75 pKa = 3.8PTEE78 pKa = 4.06YY79 pKa = 11.27GNYY82 pKa = 10.47DD83 pKa = 3.25LADD86 pKa = 3.95YY87 pKa = 11.4YY88 pKa = 11.47NDD90 pKa = 4.43SLDD93 pKa = 5.47CIDD96 pKa = 3.84KK97 pKa = 11.02LKK99 pKa = 10.47FVKK102 pKa = 10.21RR103 pKa = 11.84EE104 pKa = 3.52KK105 pKa = 10.27EE106 pKa = 3.68RR107 pKa = 11.84RR108 pKa = 11.84LKK110 pKa = 10.78EE111 pKa = 3.6EE112 pKa = 3.93EE113 pKa = 3.56EE114 pKa = 4.07RR115 pKa = 11.84QRR117 pKa = 11.84RR118 pKa = 11.84TTSRR122 pKa = 11.84EE123 pKa = 3.63SFGPSEE129 pKa = 4.08SEE131 pKa = 3.74ARR133 pKa = 11.84AKK135 pKa = 10.83VDD137 pKa = 4.09DD138 pKa = 4.27ATDD141 pKa = 3.02

Molecular weight: 16.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W6V5|A0A4P8W6V5_9VIRU DNA pilot protein OS=Tortoise microvirus 35 OX=2583138 PE=4 SV=1
MM1 pKa = 6.77RR2 pKa = 11.84QEE4 pKa = 4.07YY5 pKa = 9.54KK6 pKa = 10.99VSVNCWFITLTYY18 pKa = 10.81NEE20 pKa = 5.15DD21 pKa = 3.39NVPIINGEE29 pKa = 3.82KK30 pKa = 8.77TLVRR34 pKa = 11.84RR35 pKa = 11.84HH36 pKa = 5.77LQLFMKK42 pKa = 10.48RR43 pKa = 11.84LRR45 pKa = 11.84DD46 pKa = 3.58RR47 pKa = 11.84IKK49 pKa = 8.56PHH51 pKa = 7.05KK52 pKa = 9.98IRR54 pKa = 11.84FFACGEE60 pKa = 4.2YY61 pKa = 10.57GSKK64 pKa = 9.16TGRR67 pKa = 11.84PHH69 pKa = 5.1YY70 pKa = 10.41HH71 pKa = 7.0LILFNFFDD79 pKa = 5.31DD80 pKa = 4.21YY81 pKa = 11.73EE82 pKa = 4.4KK83 pKa = 11.38VNSIVSDD90 pKa = 3.59CWSLGFVLCKK100 pKa = 9.9PVVSNHH106 pKa = 4.33FHH108 pKa = 6.24YY109 pKa = 10.13VAKK112 pKa = 10.49YY113 pKa = 8.0CTDD116 pKa = 3.06MDD118 pKa = 3.92VGKK121 pKa = 8.88RR122 pKa = 11.84TSTRR126 pKa = 11.84GQGRR130 pKa = 11.84IPQFLLCSRR139 pKa = 11.84RR140 pKa = 11.84PAIGSGYY147 pKa = 10.4LSTSMVNYY155 pKa = 9.42YY156 pKa = 10.38RR157 pKa = 11.84NSLSIVARR165 pKa = 11.84LDD167 pKa = 3.33GKK169 pKa = 10.83KK170 pKa = 10.44YY171 pKa = 10.65SLPKK175 pKa = 10.03YY176 pKa = 9.92YY177 pKa = 9.72RR178 pKa = 11.84DD179 pKa = 3.74RR180 pKa = 11.84LFDD183 pKa = 5.59DD184 pKa = 5.21DD185 pKa = 4.59MKK187 pKa = 11.26CSIRR191 pKa = 11.84DD192 pKa = 3.48KK193 pKa = 10.48INQYY197 pKa = 10.27RR198 pKa = 11.84DD199 pKa = 3.32SLVSVDD205 pKa = 3.61TSVGEE210 pKa = 4.06TLRR213 pKa = 11.84LDD215 pKa = 3.48KK216 pKa = 10.99LRR218 pKa = 11.84YY219 pKa = 10.21DD220 pKa = 4.57SMNDD224 pKa = 3.26DD225 pKa = 2.68IRR227 pKa = 11.84RR228 pKa = 11.84FRR230 pKa = 11.84KK231 pKa = 9.86SFCKK235 pKa = 10.19NSKK238 pKa = 9.91LL239 pKa = 3.95

Molecular weight: 28.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1218

141

546

304.5

34.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.732 ± 2.019

0.903 ± 0.703

6.486 ± 1.037

5.337 ± 0.991

4.844 ± 0.496

5.829 ± 0.919

1.724 ± 0.444

4.926 ± 0.507

4.926 ± 1.0

8.621 ± 0.833

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.806 ± 0.315

6.24 ± 0.991

4.926 ± 0.974

4.351 ± 1.245

7.635 ± 0.765

8.128 ± 0.486

5.172 ± 0.554

5.008 ± 1.11

0.985 ± 0.327

5.419 ± 0.686
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski