Amino acid dipepetide frequency for Bacillus phage vB_BsuM-Goe3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.355AlaAla: 5.355 ± 0.525
0.335AlaCys: 0.335 ± 0.086
4.016AlaAsp: 4.016 ± 0.331
4.931AlaGlu: 4.931 ± 0.328
2.053AlaPhe: 2.053 ± 0.237
4.106AlaGly: 4.106 ± 0.438
0.87AlaHis: 0.87 ± 0.138
3.704AlaIle: 3.704 ± 0.269
4.44AlaLys: 4.44 ± 0.274
5.735AlaLeu: 5.735 ± 0.342
1.473AlaMet: 1.473 ± 0.163
2.945AlaAsn: 2.945 ± 0.314
2.142AlaPro: 2.142 ± 0.223
2.655AlaGln: 2.655 ± 0.279
2.856AlaArg: 2.856 ± 0.24
3.793AlaSer: 3.793 ± 0.366
3.637AlaThr: 3.637 ± 0.317
4.15AlaVal: 4.15 ± 0.376
0.513AlaTrp: 0.513 ± 0.095
2.7AlaTyr: 2.7 ± 0.218
0.0AlaXaa: 0.0 ± 0.0
Cys
0.335CysAla: 0.335 ± 0.09
0.089CysCys: 0.089 ± 0.044
0.647CysAsp: 0.647 ± 0.122
0.491CysGlu: 0.491 ± 0.101
0.357CysPhe: 0.357 ± 0.094
0.692CysGly: 0.692 ± 0.157
0.156CysHis: 0.156 ± 0.06
0.402CysIle: 0.402 ± 0.094
0.714CysLys: 0.714 ± 0.129
0.491CysLeu: 0.491 ± 0.099
0.268CysMet: 0.268 ± 0.073
0.402CysAsn: 0.402 ± 0.099
0.29CysPro: 0.29 ± 0.084
0.201CysGln: 0.201 ± 0.058
0.357CysArg: 0.357 ± 0.088
0.446CysSer: 0.446 ± 0.084
0.692CysThr: 0.692 ± 0.135
0.491CysVal: 0.491 ± 0.105
0.112CysTrp: 0.112 ± 0.051
0.312CysTyr: 0.312 ± 0.092
0.0CysXaa: 0.0 ± 0.0
Asp
3.726AspAla: 3.726 ± 0.329
0.625AspCys: 0.625 ± 0.139
3.548AspAsp: 3.548 ± 0.332
4.753AspGlu: 4.753 ± 0.299
3.191AspPhe: 3.191 ± 0.312
4.128AspGly: 4.128 ± 0.408
1.116AspHis: 1.116 ± 0.179
4.864AspIle: 4.864 ± 0.384
4.641AspLys: 4.641 ± 0.317
6.114AspLeu: 6.114 ± 0.344
2.142AspMet: 2.142 ± 0.243
3.526AspAsn: 3.526 ± 0.273
2.209AspPro: 2.209 ± 0.237
2.12AspGln: 2.12 ± 0.203
3.526AspArg: 3.526 ± 0.28
3.905AspSer: 3.905 ± 0.36
4.061AspThr: 4.061 ± 0.275
4.73AspVal: 4.73 ± 0.363
0.692AspTrp: 0.692 ± 0.115
3.012AspTyr: 3.012 ± 0.26
0.0AspXaa: 0.0 ± 0.0
Glu
5.043GluAla: 5.043 ± 0.386
0.469GluCys: 0.469 ± 0.117
5.779GluAsp: 5.779 ± 0.331
9.796GluGlu: 9.796 ± 0.864
3.012GluPhe: 3.012 ± 0.319
5.935GluGly: 5.935 ± 0.38
1.428GluHis: 1.428 ± 0.194
5.266GluIle: 5.266 ± 0.436
5.779GluLys: 5.779 ± 0.447
7.497GluLeu: 7.497 ± 0.459
2.432GluMet: 2.432 ± 0.268
3.749GluAsn: 3.749 ± 0.297
1.74GluPro: 1.74 ± 0.227
3.079GluGln: 3.079 ± 0.271
3.102GluArg: 3.102 ± 0.316
4.039GluSer: 4.039 ± 0.324
3.213GluThr: 3.213 ± 0.287
6.069GluVal: 6.069 ± 0.382
1.16GluTrp: 1.16 ± 0.179
3.325GluTyr: 3.325 ± 0.324
0.0GluXaa: 0.0 ± 0.0
Phe
2.008PheAla: 2.008 ± 0.232
0.424PheCys: 0.424 ± 0.102
2.097PheAsp: 2.097 ± 0.223
2.432PheGlu: 2.432 ± 0.245
1.361PhePhe: 1.361 ± 0.183
2.231PheGly: 2.231 ± 0.257
0.736PheHis: 0.736 ± 0.138
2.968PheIle: 2.968 ± 0.277
2.678PheLys: 2.678 ± 0.226
2.878PheLeu: 2.878 ± 0.258
0.87PheMet: 0.87 ± 0.131
2.276PheAsn: 2.276 ± 0.228
1.205PhePro: 1.205 ± 0.153
1.361PheGln: 1.361 ± 0.192
1.651PheArg: 1.651 ± 0.178
2.945PheSer: 2.945 ± 0.263
2.811PheThr: 2.811 ± 0.248
2.7PheVal: 2.7 ± 0.291
0.379PheTrp: 0.379 ± 0.099
1.54PheTyr: 1.54 ± 0.179
0.0PheXaa: 0.0 ± 0.0
Gly
4.039GlyAla: 4.039 ± 0.555
0.781GlyCys: 0.781 ± 0.15
3.994GlyAsp: 3.994 ± 0.322
4.887GlyGlu: 4.887 ± 0.337
2.231GlyPhe: 2.231 ± 0.212
5.177GlyGly: 5.177 ± 0.631
1.138GlyHis: 1.138 ± 0.207
4.039GlyIle: 4.039 ± 0.371
5.378GlyLys: 5.378 ± 0.354
4.641GlyLeu: 4.641 ± 0.396
1.696GlyMet: 1.696 ± 0.18
3.124GlyAsn: 3.124 ± 0.309
0.803GlyPro: 0.803 ± 0.127
1.986GlyGln: 1.986 ± 0.24
3.057GlyArg: 3.057 ± 0.229
4.306GlySer: 4.306 ± 0.389
3.927GlyThr: 3.927 ± 0.359
5.958GlyVal: 5.958 ± 0.344
0.803GlyTrp: 0.803 ± 0.145
3.459GlyTyr: 3.459 ± 0.307
0.0GlyXaa: 0.0 ± 0.0
His
0.848HisAla: 0.848 ± 0.143
0.112HisCys: 0.112 ± 0.046
0.893HisAsp: 0.893 ± 0.159
1.138HisGlu: 1.138 ± 0.169
0.625HisPhe: 0.625 ± 0.107
1.093HisGly: 1.093 ± 0.124
0.402HisHis: 0.402 ± 0.099
1.272HisIle: 1.272 ± 0.19
1.294HisLys: 1.294 ± 0.189
1.651HisLeu: 1.651 ± 0.214
0.602HisMet: 0.602 ± 0.117
0.781HisAsn: 0.781 ± 0.145
0.58HisPro: 0.58 ± 0.134
0.491HisGln: 0.491 ± 0.085
1.071HisArg: 1.071 ± 0.17
1.294HisSer: 1.294 ± 0.152
1.093HisThr: 1.093 ± 0.174
1.339HisVal: 1.339 ± 0.171
0.245HisTrp: 0.245 ± 0.08
1.071HisTyr: 1.071 ± 0.161
0.0HisXaa: 0.0 ± 0.0
Ile
4.351IleAla: 4.351 ± 0.318
0.424IleCys: 0.424 ± 0.109
4.931IleAsp: 4.931 ± 0.312
4.909IleGlu: 4.909 ± 0.429
1.874IlePhe: 1.874 ± 0.194
3.704IleGly: 3.704 ± 0.307
1.16IleHis: 1.16 ± 0.142
3.481IleIle: 3.481 ± 0.283
4.931IleLys: 4.931 ± 0.31
4.351IleLeu: 4.351 ± 0.387
1.495IleMet: 1.495 ± 0.168
3.213IleAsn: 3.213 ± 0.299
2.7IlePro: 2.7 ± 0.315
2.477IleGln: 2.477 ± 0.259
3.102IleArg: 3.102 ± 0.258
4.217IleSer: 4.217 ± 0.317
4.44IleThr: 4.44 ± 0.409
3.816IleVal: 3.816 ± 0.304
0.335IleTrp: 0.335 ± 0.092
1.852IleTyr: 1.852 ± 0.179
0.0IleXaa: 0.0 ± 0.0
Lys
4.775LysAla: 4.775 ± 0.322
0.424LysCys: 0.424 ± 0.103
4.909LysAsp: 4.909 ± 0.332
7.163LysGlu: 7.163 ± 0.486
2.566LysPhe: 2.566 ± 0.265
4.82LysGly: 4.82 ± 0.323
1.584LysHis: 1.584 ± 0.18
3.771LysIle: 3.771 ± 0.296
5.801LysLys: 5.801 ± 0.532
6.315LysLeu: 6.315 ± 0.404
2.432LysMet: 2.432 ± 0.265
3.771LysAsn: 3.771 ± 0.34
2.298LysPro: 2.298 ± 0.226
2.477LysGln: 2.477 ± 0.206
3.481LysArg: 3.481 ± 0.357
4.954LysSer: 4.954 ± 0.45
3.86LysThr: 3.86 ± 0.3
5.489LysVal: 5.489 ± 0.365
0.736LysTrp: 0.736 ± 0.131
2.856LysTyr: 2.856 ± 0.268
0.0LysXaa: 0.0 ± 0.0
Leu
4.842LeuAla: 4.842 ± 0.332
1.049LeuCys: 1.049 ± 0.188
6.092LeuAsp: 6.092 ± 0.361
7.096LeuGlu: 7.096 ± 0.471
3.325LeuPhe: 3.325 ± 0.273
5.11LeuGly: 5.11 ± 0.334
1.807LeuHis: 1.807 ± 0.188
4.262LeuIle: 4.262 ± 0.293
6.739LeuLys: 6.739 ± 0.371
6.582LeuLeu: 6.582 ± 0.408
1.897LeuMet: 1.897 ± 0.225
4.597LeuAsn: 4.597 ± 0.321
2.811LeuPro: 2.811 ± 0.302
3.102LeuGln: 3.102 ± 0.286
4.351LeuArg: 4.351 ± 0.387
5.757LeuSer: 5.757 ± 0.369
5.4LeuThr: 5.4 ± 0.342
5.244LeuVal: 5.244 ± 0.374
0.803LeuTrp: 0.803 ± 0.158
3.347LeuTyr: 3.347 ± 0.269
0.0LeuXaa: 0.0 ± 0.0
Met
1.74MetAla: 1.74 ± 0.183
0.179MetCys: 0.179 ± 0.057
2.053MetAsp: 2.053 ± 0.166
2.075MetGlu: 2.075 ± 0.199
0.982MetPhe: 0.982 ± 0.145
1.495MetGly: 1.495 ± 0.171
0.29MetHis: 0.29 ± 0.072
1.183MetIle: 1.183 ± 0.178
2.365MetLys: 2.365 ± 0.212
2.075MetLeu: 2.075 ± 0.241
0.424MetMet: 0.424 ± 0.091
1.517MetAsn: 1.517 ± 0.191
0.669MetPro: 0.669 ± 0.12
0.803MetGln: 0.803 ± 0.157
1.049MetArg: 1.049 ± 0.167
2.298MetSer: 2.298 ± 0.225
1.964MetThr: 1.964 ± 0.204
1.272MetVal: 1.272 ± 0.173
0.156MetTrp: 0.156 ± 0.065
0.982MetTyr: 0.982 ± 0.142
0.0MetXaa: 0.0 ± 0.0
Asn
2.678AsnAla: 2.678 ± 0.22
0.357AsnCys: 0.357 ± 0.081
2.856AsnAsp: 2.856 ± 0.252
3.012AsnGlu: 3.012 ± 0.259
1.629AsnPhe: 1.629 ± 0.193
3.548AsnGly: 3.548 ± 0.42
0.982AsnHis: 0.982 ± 0.153
3.659AsnIle: 3.659 ± 0.279
3.883AsnLys: 3.883 ± 0.345
3.927AsnLeu: 3.927 ± 0.268
1.294AsnMet: 1.294 ± 0.153
3.035AsnAsn: 3.035 ± 0.289
2.521AsnPro: 2.521 ± 0.282
1.495AsnGln: 1.495 ± 0.178
2.432AsnArg: 2.432 ± 0.262
3.459AsnSer: 3.459 ± 0.385
3.191AsnThr: 3.191 ± 0.312
3.392AsnVal: 3.392 ± 0.272
0.558AsnTrp: 0.558 ± 0.117
1.986AsnTyr: 1.986 ± 0.199
0.0AsnXaa: 0.0 ± 0.0
Pro
2.231ProAla: 2.231 ± 0.245
0.268ProCys: 0.268 ± 0.085
2.655ProAsp: 2.655 ± 0.244
3.481ProGlu: 3.481 ± 0.399
1.316ProPhe: 1.316 ± 0.193
0.893ProGly: 0.893 ± 0.166
0.692ProHis: 0.692 ± 0.145
1.874ProIle: 1.874 ± 0.178
2.097ProLys: 2.097 ± 0.228
2.767ProLeu: 2.767 ± 0.268
0.669ProMet: 0.669 ± 0.141
1.674ProAsn: 1.674 ± 0.212
0.826ProPro: 0.826 ± 0.164
1.071ProGln: 1.071 ± 0.203
1.294ProArg: 1.294 ± 0.149
2.12ProSer: 2.12 ± 0.201
2.231ProThr: 2.231 ± 0.293
2.008ProVal: 2.008 ± 0.224
0.245ProTrp: 0.245 ± 0.077
1.383ProTyr: 1.383 ± 0.172
0.0ProXaa: 0.0 ± 0.0
Gln
2.454GlnAla: 2.454 ± 0.257
0.223GlnCys: 0.223 ± 0.062
1.964GlnAsp: 1.964 ± 0.221
3.146GlnGlu: 3.146 ± 0.273
1.383GlnPhe: 1.383 ± 0.182
1.986GlnGly: 1.986 ± 0.241
0.558GlnHis: 0.558 ± 0.115
2.008GlnIle: 2.008 ± 0.216
2.7GlnLys: 2.7 ± 0.224
3.592GlnLeu: 3.592 ± 0.312
1.093GlnMet: 1.093 ± 0.146
1.473GlnAsn: 1.473 ± 0.199
1.205GlnPro: 1.205 ± 0.283
2.031GlnGln: 2.031 ± 0.426
1.807GlnArg: 1.807 ± 0.203
2.388GlnSer: 2.388 ± 0.295
1.562GlnThr: 1.562 ± 0.167
2.566GlnVal: 2.566 ± 0.228
0.245GlnTrp: 0.245 ± 0.077
1.517GlnTyr: 1.517 ± 0.169
0.0GlnXaa: 0.0 ± 0.0
Arg
3.012ArgAla: 3.012 ± 0.286
0.357ArgCys: 0.357 ± 0.086
3.102ArgAsp: 3.102 ± 0.306
4.284ArgGlu: 4.284 ± 0.311
1.897ArgPhe: 1.897 ± 0.207
3.436ArgGly: 3.436 ± 0.331
0.602ArgHis: 0.602 ± 0.096
3.012ArgIle: 3.012 ± 0.283
3.392ArgLys: 3.392 ± 0.293
4.217ArgLeu: 4.217 ± 0.373
1.607ArgMet: 1.607 ± 0.234
1.897ArgAsn: 1.897 ± 0.25
1.049ArgPro: 1.049 ± 0.159
1.718ArgGln: 1.718 ± 0.196
2.031ArgArg: 2.031 ± 0.226
2.745ArgSer: 2.745 ± 0.29
2.41ArgThr: 2.41 ± 0.315
3.793ArgVal: 3.793 ± 0.282
0.357ArgTrp: 0.357 ± 0.081
1.83ArgTyr: 1.83 ± 0.218
0.0ArgXaa: 0.0 ± 0.0
Ser
3.726SerAla: 3.726 ± 0.256
0.491SerCys: 0.491 ± 0.118
3.883SerAsp: 3.883 ± 0.367
4.485SerGlu: 4.485 ± 0.415
3.057SerPhe: 3.057 ± 0.224
4.82SerGly: 4.82 ± 0.437
0.982SerHis: 0.982 ± 0.172
4.44SerIle: 4.44 ± 0.267
4.954SerLys: 4.954 ± 0.364
5.935SerLeu: 5.935 ± 0.332
1.45SerMet: 1.45 ± 0.165
2.856SerAsn: 2.856 ± 0.328
2.187SerPro: 2.187 ± 0.199
1.986SerGln: 1.986 ± 0.221
2.789SerArg: 2.789 ± 0.242
5.511SerSer: 5.511 ± 0.395
3.927SerThr: 3.927 ± 0.421
4.686SerVal: 4.686 ± 0.298
0.759SerTrp: 0.759 ± 0.133
2.655SerTyr: 2.655 ± 0.268
0.0SerXaa: 0.0 ± 0.0
Thr
3.704ThrAla: 3.704 ± 0.344
0.245ThrCys: 0.245 ± 0.084
3.816ThrAsp: 3.816 ± 0.322
4.373ThrGlu: 4.373 ± 0.294
2.097ThrPhe: 2.097 ± 0.231
4.44ThrGly: 4.44 ± 0.375
1.183ThrHis: 1.183 ± 0.147
3.659ThrIle: 3.659 ± 0.377
4.262ThrLys: 4.262 ± 0.31
5.378ThrLeu: 5.378 ± 0.336
1.138ThrMet: 1.138 ± 0.164
2.7ThrAsn: 2.7 ± 0.28
2.878ThrPro: 2.878 ± 0.258
2.097ThrGln: 2.097 ± 0.241
2.901ThrArg: 2.901 ± 0.262
3.548ThrSer: 3.548 ± 0.311
3.749ThrThr: 3.749 ± 0.319
4.976ThrVal: 4.976 ± 0.333
0.513ThrTrp: 0.513 ± 0.102
2.811ThrTyr: 2.811 ± 0.26
0.0ThrXaa: 0.0 ± 0.0
Val
4.262ValAla: 4.262 ± 0.327
0.647ValCys: 0.647 ± 0.138
5.601ValAsp: 5.601 ± 0.329
5.199ValGlu: 5.199 ± 0.367
3.035ValPhe: 3.035 ± 0.273
4.15ValGly: 4.15 ± 0.282
1.428ValHis: 1.428 ± 0.2
4.016ValIle: 4.016 ± 0.29
5.154ValLys: 5.154 ± 0.372
5.333ValLeu: 5.333 ± 0.442
1.629ValMet: 1.629 ± 0.196
3.749ValAsn: 3.749 ± 0.412
2.432ValPro: 2.432 ± 0.246
2.745ValGln: 2.745 ± 0.24
3.258ValArg: 3.258 ± 0.281
4.507ValSer: 4.507 ± 0.331
4.686ValThr: 4.686 ± 0.341
5.266ValVal: 5.266 ± 0.441
0.803ValTrp: 0.803 ± 0.142
3.57ValTyr: 3.57 ± 0.284
0.0ValXaa: 0.0 ± 0.0
Trp
0.513TrpAla: 0.513 ± 0.111
0.156TrpCys: 0.156 ± 0.062
0.826TrpAsp: 0.826 ± 0.139
0.87TrpGlu: 0.87 ± 0.132
0.446TrpPhe: 0.446 ± 0.096
0.803TrpGly: 0.803 ± 0.13
0.179TrpHis: 0.179 ± 0.061
0.602TrpIle: 0.602 ± 0.126
0.602TrpLys: 0.602 ± 0.114
0.848TrpLeu: 0.848 ± 0.15
0.134TrpMet: 0.134 ± 0.057
0.424TrpAsn: 0.424 ± 0.105
0.0TrpPro: 0.0 ± 0.0
0.312TrpGln: 0.312 ± 0.084
0.536TrpArg: 0.536 ± 0.115
0.58TrpSer: 0.58 ± 0.14
0.602TrpThr: 0.602 ± 0.111
1.026TrpVal: 1.026 ± 0.176
0.223TrpTrp: 0.223 ± 0.085
0.379TrpTyr: 0.379 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.767TyrAla: 2.767 ± 0.241
0.29TyrCys: 0.29 ± 0.081
2.968TyrAsp: 2.968 ± 0.259
3.392TyrGlu: 3.392 ± 0.257
1.138TyrPhe: 1.138 ± 0.159
2.856TyrGly: 2.856 ± 0.26
0.602TyrHis: 0.602 ± 0.107
3.28TyrIle: 3.28 ± 0.263
2.678TyrLys: 2.678 ± 0.217
3.883TyrLeu: 3.883 ± 0.324
0.803TyrMet: 0.803 ± 0.129
2.187TyrAsn: 2.187 ± 0.257
1.25TyrPro: 1.25 ± 0.2
1.696TyrGln: 1.696 ± 0.222
2.097TyrArg: 2.097 ± 0.204
2.767TyrSer: 2.767 ± 0.257
3.012TyrThr: 3.012 ± 0.324
2.499TyrVal: 2.499 ± 0.263
0.424TyrTrp: 0.424 ± 0.108
1.696TyrTyr: 1.696 ± 0.234
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 236 proteins (44817 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski