Amino acid dipepetide frequency for Erwinia phage vB_EamM_Alexandra

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.459AlaAla: 8.459 ± 0.494
0.844AlaCys: 0.844 ± 0.092
5.471AlaAsp: 5.471 ± 0.266
5.506AlaGlu: 5.506 ± 0.281
3.175AlaPhe: 3.175 ± 0.17
5.12AlaGly: 5.12 ± 0.323
1.535AlaHis: 1.535 ± 0.133
4.206AlaIle: 4.206 ± 0.241
5.741AlaLys: 5.741 ± 0.417
7.639AlaLeu: 7.639 ± 0.336
2.706AlaMet: 2.706 ± 0.177
4.1AlaAsn: 4.1 ± 0.227
3.468AlaPro: 3.468 ± 0.264
3.116AlaGln: 3.116 ± 0.193
4.264AlaArg: 4.264 ± 0.223
5.061AlaSer: 5.061 ± 0.294
4.792AlaThr: 4.792 ± 0.291
5.799AlaVal: 5.799 ± 0.308
0.867AlaTrp: 0.867 ± 0.104
2.894AlaTyr: 2.894 ± 0.197
0.0AlaXaa: 0.0 ± 0.0
Cys
0.867CysAla: 0.867 ± 0.1
0.223CysCys: 0.223 ± 0.047
0.926CysAsp: 0.926 ± 0.103
0.762CysGlu: 0.762 ± 0.1
0.492CysPhe: 0.492 ± 0.071
0.972CysGly: 0.972 ± 0.113
0.293CysHis: 0.293 ± 0.063
0.527CysIle: 0.527 ± 0.07
0.644CysLys: 0.644 ± 0.093
0.972CysLeu: 0.972 ± 0.116
0.34CysMet: 0.34 ± 0.061
0.387CysAsn: 0.387 ± 0.066
0.726CysPro: 0.726 ± 0.085
0.363CysGln: 0.363 ± 0.055
0.762CysArg: 0.762 ± 0.094
0.715CysSer: 0.715 ± 0.101
0.551CysThr: 0.551 ± 0.076
0.762CysVal: 0.762 ± 0.084
0.152CysTrp: 0.152 ± 0.04
0.398CysTyr: 0.398 ± 0.074
0.0CysXaa: 0.0 ± 0.0
Asp
5.495AspAla: 5.495 ± 0.239
0.867AspCys: 0.867 ± 0.104
5.354AspAsp: 5.354 ± 0.624
5.588AspGlu: 5.588 ± 0.63
2.695AspPhe: 2.695 ± 0.198
4.674AspGly: 4.674 ± 0.305
1.148AspHis: 1.148 ± 0.114
3.854AspIle: 3.854 ± 0.239
3.163AspLys: 3.163 ± 0.251
6.162AspLeu: 6.162 ± 0.275
2.179AspMet: 2.179 ± 0.151
2.659AspAsn: 2.659 ± 0.161
3.023AspPro: 3.023 ± 0.192
2.413AspGln: 2.413 ± 0.164
2.613AspArg: 2.613 ± 0.18
3.901AspSer: 3.901 ± 0.21
3.655AspThr: 3.655 ± 0.189
4.674AspVal: 4.674 ± 0.244
0.996AspTrp: 0.996 ± 0.124
2.73AspTyr: 2.73 ± 0.197
0.0AspXaa: 0.0 ± 0.0
Glu
4.628GluAla: 4.628 ± 0.274
0.656GluCys: 0.656 ± 0.084
4.85GluAsp: 4.85 ± 0.588
4.546GluGlu: 4.546 ± 0.507
2.238GluPhe: 2.238 ± 0.183
3.444GluGly: 3.444 ± 0.2
1.734GluHis: 1.734 ± 0.142
3.327GluIle: 3.327 ± 0.186
3.386GluLys: 3.386 ± 0.248
5.612GluLeu: 5.612 ± 0.27
1.792GluMet: 1.792 ± 0.153
2.425GluAsn: 2.425 ± 0.168
2.097GluPro: 2.097 ± 0.162
3.034GluGln: 3.034 ± 0.192
3.304GluArg: 3.304 ± 0.221
3.269GluSer: 3.269 ± 0.217
3.034GluThr: 3.034 ± 0.207
3.972GluVal: 3.972 ± 0.237
0.773GluTrp: 0.773 ± 0.085
2.121GluTyr: 2.121 ± 0.162
0.0GluXaa: 0.0 ± 0.0
Phe
2.741PheAla: 2.741 ± 0.164
0.562PheCys: 0.562 ± 0.085
4.171PheAsp: 4.171 ± 0.243
2.437PheGlu: 2.437 ± 0.171
1.336PhePhe: 1.336 ± 0.128
2.847PheGly: 2.847 ± 0.152
0.586PheHis: 0.586 ± 0.084
2.121PheIle: 2.121 ± 0.164
2.132PheLys: 2.132 ± 0.185
2.636PheLeu: 2.636 ± 0.184
0.984PheMet: 0.984 ± 0.111
2.132PheAsn: 2.132 ± 0.172
1.593PhePro: 1.593 ± 0.118
1.054PheGln: 1.054 ± 0.117
2.191PheArg: 2.191 ± 0.162
2.577PheSer: 2.577 ± 0.171
2.542PheThr: 2.542 ± 0.165
2.882PheVal: 2.882 ± 0.206
0.41PheTrp: 0.41 ± 0.065
1.031PheTyr: 1.031 ± 0.108
0.0PheXaa: 0.0 ± 0.0
Gly
5.108GlyAla: 5.108 ± 0.273
0.609GlyCys: 0.609 ± 0.085
4.089GlyAsp: 4.089 ± 0.223
3.772GlyGlu: 3.772 ± 0.199
2.413GlyPhe: 2.413 ± 0.162
4.733GlyGly: 4.733 ± 0.303
1.277GlyHis: 1.277 ± 0.145
3.151GlyIle: 3.151 ± 0.201
4.616GlyLys: 4.616 ± 0.281
5.237GlyLeu: 5.237 ± 0.238
1.792GlyMet: 1.792 ± 0.13
2.683GlyAsn: 2.683 ± 0.187
2.413GlyPro: 2.413 ± 0.267
2.589GlyGln: 2.589 ± 0.202
3.468GlyArg: 3.468 ± 0.288
4.311GlySer: 4.311 ± 0.23
4.651GlyThr: 4.651 ± 0.29
4.534GlyVal: 4.534 ± 0.209
0.949GlyTrp: 0.949 ± 0.12
2.308GlyTyr: 2.308 ± 0.151
0.0GlyXaa: 0.0 ± 0.0
His
1.5HisAla: 1.5 ± 0.14
0.293HisCys: 0.293 ± 0.057
1.172HisAsp: 1.172 ± 0.129
1.277HisGlu: 1.277 ± 0.122
0.949HisPhe: 0.949 ± 0.098
1.441HisGly: 1.441 ± 0.141
0.597HisHis: 0.597 ± 0.089
1.207HisIle: 1.207 ± 0.131
1.207HisLys: 1.207 ± 0.115
1.664HisLeu: 1.664 ± 0.128
0.574HisMet: 0.574 ± 0.083
0.902HisAsn: 0.902 ± 0.103
1.136HisPro: 1.136 ± 0.111
0.679HisGln: 0.679 ± 0.087
1.277HisArg: 1.277 ± 0.127
1.113HisSer: 1.113 ± 0.117
1.172HisThr: 1.172 ± 0.103
1.523HisVal: 1.523 ± 0.144
0.34HisTrp: 0.34 ± 0.067
0.668HisTyr: 0.668 ± 0.094
0.0HisXaa: 0.0 ± 0.0
Ile
4.897IleAla: 4.897 ± 0.23
0.738IleCys: 0.738 ± 0.09
4.124IleAsp: 4.124 ± 0.238
3.761IleGlu: 3.761 ± 0.22
1.64IlePhe: 1.64 ± 0.142
3.28IleGly: 3.28 ± 0.196
1.23IleHis: 1.23 ± 0.116
2.659IleIle: 2.659 ± 0.2
3.269IleLys: 3.269 ± 0.238
3.538IleLeu: 3.538 ± 0.197
1.488IleMet: 1.488 ± 0.12
2.952IleAsn: 2.952 ± 0.179
2.519IlePro: 2.519 ± 0.192
1.968IleGln: 1.968 ± 0.146
3.081IleArg: 3.081 ± 0.201
3.257IleSer: 3.257 ± 0.196
3.14IleThr: 3.14 ± 0.22
3.96IleVal: 3.96 ± 0.235
0.398IleTrp: 0.398 ± 0.067
1.5IleTyr: 1.5 ± 0.114
0.0IleXaa: 0.0 ± 0.0
Lys
6.127LysAla: 6.127 ± 0.408
0.644LysCys: 0.644 ± 0.101
3.409LysAsp: 3.409 ± 0.228
3.292LysGlu: 3.292 ± 0.235
2.038LysPhe: 2.038 ± 0.15
3.62LysGly: 3.62 ± 0.235
1.453LysHis: 1.453 ± 0.145
2.929LysIle: 2.929 ± 0.216
5.319LysLys: 5.319 ± 0.594
5.167LysLeu: 5.167 ± 0.319
1.851LysMet: 1.851 ± 0.187
2.179LysAsn: 2.179 ± 0.166
3.081LysPro: 3.081 ± 0.248
2.495LysGln: 2.495 ± 0.185
3.714LysArg: 3.714 ± 0.254
3.737LysSer: 3.737 ± 0.24
3.749LysThr: 3.749 ± 0.224
3.89LysVal: 3.89 ± 0.222
0.656LysTrp: 0.656 ± 0.081
2.109LysTyr: 2.109 ± 0.164
0.0LysXaa: 0.0 ± 0.0
Leu
6.502LeuAla: 6.502 ± 0.233
0.937LeuCys: 0.937 ± 0.113
5.846LeuAsp: 5.846 ± 0.296
4.475LeuGlu: 4.475 ± 0.213
3.046LeuPhe: 3.046 ± 0.228
5.12LeuGly: 5.12 ± 0.242
1.582LeuHis: 1.582 ± 0.142
4.71LeuIle: 4.71 ± 0.247
5.108LeuLys: 5.108 ± 0.346
6.174LeuLeu: 6.174 ± 0.293
2.46LeuMet: 2.46 ± 0.175
4.346LeuAsn: 4.346 ± 0.218
4.241LeuPro: 4.241 ± 0.189
2.542LeuGln: 2.542 ± 0.166
5.155LeuArg: 5.155 ± 0.224
6.244LeuSer: 6.244 ± 0.275
5.541LeuThr: 5.541 ± 0.261
5.53LeuVal: 5.53 ± 0.279
0.773LeuTrp: 0.773 ± 0.099
2.519LeuTyr: 2.519 ± 0.172
0.0LeuXaa: 0.0 ± 0.0
Met
2.331MetAla: 2.331 ± 0.145
0.351MetCys: 0.351 ± 0.05
1.406MetAsp: 1.406 ± 0.116
1.5MetGlu: 1.5 ± 0.133
1.429MetPhe: 1.429 ± 0.129
1.476MetGly: 1.476 ± 0.138
0.574MetHis: 0.574 ± 0.09
1.57MetIle: 1.57 ± 0.134
1.98MetLys: 1.98 ± 0.168
2.882MetLeu: 2.882 ± 0.181
0.797MetMet: 0.797 ± 0.098
1.453MetAsn: 1.453 ± 0.123
1.628MetPro: 1.628 ± 0.13
1.359MetGln: 1.359 ± 0.105
1.746MetArg: 1.746 ± 0.151
1.98MetSer: 1.98 ± 0.15
1.956MetThr: 1.956 ± 0.153
1.336MetVal: 1.336 ± 0.106
0.375MetTrp: 0.375 ± 0.066
0.996MetTyr: 0.996 ± 0.099
0.0MetXaa: 0.0 ± 0.0
Asn
4.194AsnAla: 4.194 ± 0.247
0.492AsnCys: 0.492 ± 0.07
2.8AsnAsp: 2.8 ± 0.158
2.121AsnGlu: 2.121 ± 0.162
1.898AsnPhe: 1.898 ± 0.156
3.608AsnGly: 3.608 ± 0.209
0.785AsnHis: 0.785 ± 0.102
2.624AsnIle: 2.624 ± 0.195
2.671AsnLys: 2.671 ± 0.195
3.315AsnLeu: 3.315 ± 0.215
1.523AsnMet: 1.523 ± 0.156
2.32AsnAsn: 2.32 ± 0.201
2.179AsnPro: 2.179 ± 0.145
1.675AsnGln: 1.675 ± 0.167
2.062AsnArg: 2.062 ± 0.172
2.765AsnSer: 2.765 ± 0.194
3.222AsnThr: 3.222 ± 0.193
3.585AsnVal: 3.585 ± 0.213
0.398AsnTrp: 0.398 ± 0.065
1.324AsnTyr: 1.324 ± 0.129
0.0AsnXaa: 0.0 ± 0.0
Pro
3.796ProAla: 3.796 ± 0.251
0.515ProCys: 0.515 ± 0.08
3.351ProAsp: 3.351 ± 0.222
3.128ProGlu: 3.128 ± 0.181
1.734ProPhe: 1.734 ± 0.149
2.273ProGly: 2.273 ± 0.222
0.879ProHis: 0.879 ± 0.092
2.413ProIle: 2.413 ± 0.163
3.046ProLys: 3.046 ± 0.234
3.105ProLeu: 3.105 ± 0.209
1.078ProMet: 1.078 ± 0.116
2.027ProAsn: 2.027 ± 0.142
1.289ProPro: 1.289 ± 0.127
1.593ProGln: 1.593 ± 0.148
1.98ProArg: 1.98 ± 0.136
2.589ProSer: 2.589 ± 0.177
3.245ProThr: 3.245 ± 0.212
3.948ProVal: 3.948 ± 0.199
0.375ProTrp: 0.375 ± 0.062
1.441ProTyr: 1.441 ± 0.122
0.0ProXaa: 0.0 ± 0.0
Gln
2.941GlnAla: 2.941 ± 0.204
0.375GlnCys: 0.375 ± 0.062
1.863GlnAsp: 1.863 ± 0.137
2.027GlnGlu: 2.027 ± 0.153
1.828GlnPhe: 1.828 ± 0.137
2.238GlnGly: 2.238 ± 0.171
0.82GlnHis: 0.82 ± 0.12
2.402GlnIle: 2.402 ± 0.181
1.968GlnLys: 1.968 ± 0.151
3.491GlnLeu: 3.491 ± 0.214
1.429GlnMet: 1.429 ± 0.124
1.535GlnAsn: 1.535 ± 0.13
1.546GlnPro: 1.546 ± 0.152
1.898GlnGln: 1.898 ± 0.151
2.331GlnArg: 2.331 ± 0.197
2.238GlnSer: 2.238 ± 0.166
2.472GlnThr: 2.472 ± 0.202
2.296GlnVal: 2.296 ± 0.148
0.574GlnTrp: 0.574 ± 0.072
1.687GlnTyr: 1.687 ± 0.121
0.0GlnXaa: 0.0 ± 0.0
Arg
4.253ArgAla: 4.253 ± 0.251
0.586ArgCys: 0.586 ± 0.085
3.269ArgAsp: 3.269 ± 0.231
3.093ArgGlu: 3.093 ± 0.209
2.449ArgPhe: 2.449 ± 0.18
3.245ArgGly: 3.245 ± 0.229
1.148ArgHis: 1.148 ± 0.124
3.304ArgIle: 3.304 ± 0.22
3.597ArgLys: 3.597 ± 0.222
4.792ArgLeu: 4.792 ± 0.224
1.675ArgMet: 1.675 ± 0.124
2.437ArgAsn: 2.437 ± 0.174
2.226ArgPro: 2.226 ± 0.141
1.968ArgGln: 1.968 ± 0.164
3.163ArgArg: 3.163 ± 0.181
3.069ArgSer: 3.069 ± 0.202
3.151ArgThr: 3.151 ± 0.214
4.065ArgVal: 4.065 ± 0.205
0.469ArgTrp: 0.469 ± 0.075
1.898ArgTyr: 1.898 ± 0.152
0.0ArgXaa: 0.0 ± 0.0
Ser
5.459SerAla: 5.459 ± 0.288
0.82SerCys: 0.82 ± 0.089
4.288SerAsp: 4.288 ± 0.231
3.351SerGlu: 3.351 ± 0.22
2.378SerPhe: 2.378 ± 0.177
4.604SerGly: 4.604 ± 0.24
1.172SerHis: 1.172 ± 0.124
3.21SerIle: 3.21 ± 0.19
3.702SerLys: 3.702 ± 0.258
5.764SerLeu: 5.764 ± 0.329
1.652SerMet: 1.652 ± 0.16
2.882SerAsn: 2.882 ± 0.223
2.554SerPro: 2.554 ± 0.141
2.074SerGln: 2.074 ± 0.173
2.917SerArg: 2.917 ± 0.191
4.124SerSer: 4.124 ± 0.269
3.784SerThr: 3.784 ± 0.212
4.932SerVal: 4.932 ± 0.266
0.773SerTrp: 0.773 ± 0.105
2.296SerTyr: 2.296 ± 0.198
0.0SerXaa: 0.0 ± 0.0
Thr
5.682ThrAla: 5.682 ± 0.369
0.75ThrCys: 0.75 ± 0.083
3.655ThrAsp: 3.655 ± 0.21
2.812ThrGlu: 2.812 ± 0.169
2.741ThrPhe: 2.741 ± 0.178
4.546ThrGly: 4.546 ± 0.348
1.441ThrHis: 1.441 ± 0.119
3.667ThrIle: 3.667 ± 0.234
3.14ThrLys: 3.14 ± 0.204
5.331ThrLeu: 5.331 ± 0.277
1.394ThrMet: 1.394 ± 0.15
2.624ThrAsn: 2.624 ± 0.198
3.023ThrPro: 3.023 ± 0.19
2.484ThrGln: 2.484 ± 0.163
2.87ThrArg: 2.87 ± 0.168
3.644ThrSer: 3.644 ± 0.193
3.702ThrThr: 3.702 ± 0.231
5.565ThrVal: 5.565 ± 0.316
0.773ThrTrp: 0.773 ± 0.107
2.191ThrTyr: 2.191 ± 0.177
0.0ThrXaa: 0.0 ± 0.0
Val
5.869ValAla: 5.869 ± 0.294
0.855ValCys: 0.855 ± 0.093
4.897ValAsp: 4.897 ± 0.235
4.522ValGlu: 4.522 ± 0.229
2.695ValPhe: 2.695 ± 0.178
4.335ValGly: 4.335 ± 0.23
1.3ValHis: 1.3 ± 0.124
3.421ValIle: 3.421 ± 0.218
4.335ValLys: 4.335 ± 0.267
5.588ValLeu: 5.588 ± 0.298
2.027ValMet: 2.027 ± 0.136
3.339ValAsn: 3.339 ± 0.177
3.315ValPro: 3.315 ± 0.214
2.788ValGln: 2.788 ± 0.182
4.112ValArg: 4.112 ± 0.274
5.225ValSer: 5.225 ± 0.253
4.674ValThr: 4.674 ± 0.352
5.553ValVal: 5.553 ± 0.297
0.902ValTrp: 0.902 ± 0.111
2.601ValTyr: 2.601 ± 0.16
0.0ValXaa: 0.0 ± 0.0
Trp
0.89TrpAla: 0.89 ± 0.1
0.223TrpCys: 0.223 ± 0.053
0.597TrpAsp: 0.597 ± 0.096
0.621TrpGlu: 0.621 ± 0.089
0.387TrpPhe: 0.387 ± 0.064
0.668TrpGly: 0.668 ± 0.089
0.305TrpHis: 0.305 ± 0.057
0.691TrpIle: 0.691 ± 0.071
0.621TrpLys: 0.621 ± 0.093
1.043TrpLeu: 1.043 ± 0.113
0.305TrpMet: 0.305 ± 0.058
0.433TrpAsn: 0.433 ± 0.062
0.551TrpPro: 0.551 ± 0.081
0.445TrpGln: 0.445 ± 0.069
0.668TrpArg: 0.668 ± 0.087
0.914TrpSer: 0.914 ± 0.1
0.832TrpThr: 0.832 ± 0.096
0.785TrpVal: 0.785 ± 0.11
0.117TrpTrp: 0.117 ± 0.04
0.422TrpTyr: 0.422 ± 0.067
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.023TyrAla: 3.023 ± 0.2
0.492TyrCys: 0.492 ± 0.082
2.308TyrAsp: 2.308 ± 0.153
1.652TyrGlu: 1.652 ± 0.139
1.406TyrPhe: 1.406 ± 0.128
2.46TyrGly: 2.46 ± 0.183
0.832TyrHis: 0.832 ± 0.101
1.406TyrIle: 1.406 ± 0.117
1.816TyrLys: 1.816 ± 0.149
2.741TyrLeu: 2.741 ± 0.206
1.066TyrMet: 1.066 ± 0.118
1.746TyrAsn: 1.746 ± 0.124
1.195TyrPro: 1.195 ± 0.12
1.418TyrGln: 1.418 ± 0.141
2.179TyrArg: 2.179 ± 0.169
2.003TyrSer: 2.003 ± 0.191
2.179TyrThr: 2.179 ± 0.169
2.765TyrVal: 2.765 ± 0.155
0.445TyrTrp: 0.445 ± 0.06
1.172TyrTyr: 1.172 ± 0.122
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 345 proteins (85358 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski