Drosophila willistoni (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae;

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14820 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4MTW5|B4MTW5_DROWI BEN domain-containing protein OS=Drosophila willistoni OX=7260 GN=Dwil\GK23918 PE=4 SV=2
MM1 pKa = 7.31FNKK4 pKa = 10.22SYY6 pKa = 11.1TNIILASTLLLCLITLPVSQADD28 pKa = 3.74LMCYY32 pKa = 10.18VCDD35 pKa = 5.0DD36 pKa = 4.38CATMPKK42 pKa = 10.1DD43 pKa = 4.81APLLACNEE51 pKa = 4.28DD52 pKa = 3.71FFNPGGSTEE61 pKa = 4.14ASTVTTTTPASTTEE75 pKa = 4.29AVQSTTPIEE84 pKa = 4.11TSPMTIASTTEE95 pKa = 3.73EE96 pKa = 4.43SSTDD100 pKa = 3.52LASTTTEE107 pKa = 4.12EE108 pKa = 4.38VTSTSTSEE116 pKa = 3.81QSTEE120 pKa = 3.97STEE123 pKa = 4.6APIPTPNTAGPVEE136 pKa = 4.5TTTGVPTPPQEE147 pKa = 5.19DD148 pKa = 4.06LDD150 pKa = 4.23AVPASAANTTRR161 pKa = 11.84LLQIGEE167 pKa = 4.25EE168 pKa = 4.25SSSNVTPLAIRR179 pKa = 11.84QRR181 pKa = 11.84RR182 pKa = 11.84ALINTDD188 pKa = 3.47YY189 pKa = 10.98SYY191 pKa = 11.43HH192 pKa = 6.95CYY194 pKa = 9.92IVQKK198 pKa = 10.11SINGSTVTDD207 pKa = 4.24RR208 pKa = 11.84GCSRR212 pKa = 11.84VSTYY216 pKa = 10.95QSVCGLLMEE225 pKa = 5.06QNNNTQLSNCDD236 pKa = 3.59PCSMNACNGSSLLQNSLLVTFLMAAIAALMQANVLYY272 pKa = 10.84KK273 pKa = 10.46KK274 pKa = 9.65MRR276 pKa = 11.84LISLLLITLICAGATIWSANALQCYY301 pKa = 8.68EE302 pKa = 4.81CIGKK306 pKa = 9.12EE307 pKa = 3.9CDD309 pKa = 3.39EE310 pKa = 4.31LTEE313 pKa = 4.05EE314 pKa = 4.82HH315 pKa = 6.91LVSCNVDD322 pKa = 3.18NDD324 pKa = 4.13PVTTPSDD331 pKa = 3.72EE332 pKa = 4.26TDD334 pKa = 3.5STDD337 pKa = 3.33DD338 pKa = 3.68TTDD341 pKa = 2.74KK342 pKa = 10.08STPSATSSTTNGVTTSSPGTTTGTTGTTDD371 pKa = 3.6ADD373 pKa = 3.99TTTTTDD379 pKa = 2.8EE380 pKa = 5.29GYY382 pKa = 10.88SSTIEE387 pKa = 4.39DD388 pKa = 3.98EE389 pKa = 4.82SSVSTDD395 pKa = 3.92DD396 pKa = 5.49DD397 pKa = 4.41SSSSNTTDD405 pKa = 4.63DD406 pKa = 4.49DD407 pKa = 4.74SSTNTEE413 pKa = 4.13SSTSDD418 pKa = 3.03TTEE421 pKa = 4.65DD422 pKa = 4.06DD423 pKa = 3.93SSSSSNDD430 pKa = 3.21DD431 pKa = 3.46SSSSSTTEE439 pKa = 3.87STSEE443 pKa = 3.89SSPNTEE449 pKa = 4.07SSTSDD454 pKa = 3.03TTEE457 pKa = 4.65DD458 pKa = 4.06DD459 pKa = 3.93SSSSSNDD466 pKa = 3.25DD467 pKa = 3.3SSSSITTEE475 pKa = 3.9SSSEE479 pKa = 3.99SSPNAEE485 pKa = 4.24SSTSDD490 pKa = 3.07TTEE493 pKa = 4.67DD494 pKa = 4.14DD495 pKa = 3.91SSSSTNADD503 pKa = 3.08SSSSNTTDD511 pKa = 3.37EE512 pKa = 4.84DD513 pKa = 4.32SSTNTEE519 pKa = 4.09SSTSDD524 pKa = 3.03TTEE527 pKa = 4.65DD528 pKa = 4.06DD529 pKa = 3.93SSSSSNDD536 pKa = 3.04EE537 pKa = 4.06SSSSITTEE545 pKa = 4.03SASEE549 pKa = 4.01SSPNAEE555 pKa = 4.24SSTSDD560 pKa = 3.07TTEE563 pKa = 4.65DD564 pKa = 4.06DD565 pKa = 3.93SSSSSNDD572 pKa = 3.24DD573 pKa = 3.31SSSTIVTEE581 pKa = 4.33SEE583 pKa = 4.38SPSQSSIDD591 pKa = 3.96AEE593 pKa = 4.49SSTSGIIEE601 pKa = 4.95DD602 pKa = 4.74DD603 pKa = 3.7SSSASSGASEE613 pKa = 4.32SSSASSEE620 pKa = 4.25TSSSGAEE627 pKa = 3.91TTVGSTEE634 pKa = 3.96APVTSRR640 pKa = 11.84AKK642 pKa = 10.25RR643 pKa = 11.84SLAARR648 pKa = 11.84ATDD651 pKa = 3.91RR652 pKa = 11.84LLQNYY657 pKa = 9.21RR658 pKa = 11.84SYY660 pKa = 11.77ARR662 pKa = 11.84YY663 pKa = 9.59RR664 pKa = 11.84RR665 pKa = 11.84DD666 pKa = 2.93AEE668 pKa = 4.15GQIRR672 pKa = 11.84TTACYY677 pKa = 10.44SIVKK681 pKa = 10.35DD682 pKa = 3.58NVIQRR687 pKa = 11.84GCIRR691 pKa = 11.84IPYY694 pKa = 9.21GKK696 pKa = 9.79GGCEE700 pKa = 3.41AVRR703 pKa = 11.84IEE705 pKa = 4.36VNLAADD711 pKa = 4.28SIGDD715 pKa = 3.78EE716 pKa = 4.34CDD718 pKa = 2.95VCQMDD723 pKa = 4.02KK724 pKa = 11.15CNSSTSLRR732 pKa = 11.84LSLGLMLLGLITFGLRR748 pKa = 11.84SLL750 pKa = 4.12

Molecular weight: 78.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4NJF2|B4NJF2_DROWI Serine/threonine-protein phosphatase OS=Drosophila willistoni OX=7260 GN=Dwil\GK12844 PE=3 SV=1
MM1 pKa = 7.71PKK3 pKa = 10.26SLLDD7 pKa = 3.86YY8 pKa = 10.8SPHH11 pKa = 6.65GYY13 pKa = 10.23DD14 pKa = 2.71WLFLFVVFFIFAGGLGNILVCLAVALDD41 pKa = 3.71RR42 pKa = 11.84KK43 pKa = 9.47LQNVTNYY50 pKa = 10.16FLFSLAIADD59 pKa = 4.27LLVSLFVMPMGAIPAFLGYY78 pKa = 9.61WPLGFTWCNIYY89 pKa = 8.07VTCDD93 pKa = 3.17VLACSSSILHH103 pKa = 5.91MCFISLGRR111 pKa = 11.84YY112 pKa = 9.06LGIRR116 pKa = 11.84NPLGSRR122 pKa = 11.84HH123 pKa = 6.34RR124 pKa = 11.84STKK127 pKa = 9.86RR128 pKa = 11.84LAGIKK133 pKa = 9.64IAIVWVMAMMVSSSITVLGLVNKK156 pKa = 9.48HH157 pKa = 6.27NIMPEE162 pKa = 3.8PNICMINNRR171 pKa = 11.84AFWVFGSLVAFYY183 pKa = 10.53IPMLMMVTTYY193 pKa = 11.64ALTIPLLRR201 pKa = 11.84KK202 pKa = 9.14KK203 pKa = 10.66ARR205 pKa = 11.84FAAEE209 pKa = 4.06HH210 pKa = 6.84PEE212 pKa = 4.03SEE214 pKa = 4.24LFRR217 pKa = 11.84RR218 pKa = 11.84LGGRR222 pKa = 11.84FTIRR226 pKa = 11.84PQHH229 pKa = 5.79SQQQLQMHH237 pKa = 6.74SSFSSNSNKK246 pKa = 9.24FLAMSDD252 pKa = 3.36SNRR255 pKa = 11.84NFNSSDD261 pKa = 3.18RR262 pKa = 11.84GGGRR266 pKa = 11.84GGDD269 pKa = 3.44NSTEE273 pKa = 3.96RR274 pKa = 11.84PLMQQRR280 pKa = 11.84TTSNRR285 pKa = 11.84SMGAGSVSFRR295 pKa = 11.84NVTNGGRR302 pKa = 11.84TAFGVGMGAGGRR314 pKa = 11.84SSFRR318 pKa = 11.84FAGGSILRR326 pKa = 11.84QSSTPTTTSTSLSSSCPSSTATSVRR351 pKa = 11.84GGRR354 pKa = 11.84SQQTSSFWRR363 pKa = 11.84KK364 pKa = 9.57HH365 pKa = 4.85GGNPNLMDD373 pKa = 3.28SHH375 pKa = 6.74PNRR378 pKa = 11.84RR379 pKa = 11.84ASVRR383 pKa = 11.84VSMSQPQLGYY393 pKa = 7.52PTNGNGGNGGGIGGNEE409 pKa = 4.26SPTAVTSSSCTTPGSISNLVNVATIQGQSMDD440 pKa = 3.38NHH442 pKa = 5.53QLEE445 pKa = 5.09HH446 pKa = 6.09GTTMTATITSAATTAASASARR467 pKa = 11.84ATTTTTGEE475 pKa = 4.1RR476 pKa = 11.84RR477 pKa = 11.84HH478 pKa = 6.08KK479 pKa = 10.53LRR481 pKa = 11.84PFKK484 pKa = 10.6FALNRR489 pKa = 11.84VATPTLNLRR498 pKa = 11.84FLNNRR503 pKa = 11.84NKK505 pKa = 10.44RR506 pKa = 11.84NSLSANAVATEE517 pKa = 3.99QKK519 pKa = 8.69ATKK522 pKa = 10.48VLGLVFFTFVLCWSPFFILNIIFAACPQCQVSEE555 pKa = 4.41HH556 pKa = 5.38VVNTCLWLGYY566 pKa = 10.09VSSTINPIIYY576 pKa = 9.08TIFNRR581 pKa = 11.84TFRR584 pKa = 11.84AAFIRR589 pKa = 11.84LLKK592 pKa = 10.36CNCEE596 pKa = 3.68RR597 pKa = 11.84SGRR600 pKa = 11.84PLRR603 pKa = 11.84FRR605 pKa = 11.84SVTEE609 pKa = 3.85GRR611 pKa = 11.84SALSLCAPSALPLAISFQGAPLMTPSTTNATPLSEE646 pKa = 4.21FRR648 pKa = 11.84GSYY651 pKa = 10.02TITDD655 pKa = 3.95DD656 pKa = 3.51EE657 pKa = 4.72CC658 pKa = 6.48

Molecular weight: 71.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13713

1107

14820

8086281

23

20907

545.6

60.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.226 ± 0.025

1.833 ± 0.031

5.293 ± 0.017

6.423 ± 0.04

3.488 ± 0.017

5.964 ± 0.026

2.651 ± 0.012

5.192 ± 0.016

5.688 ± 0.028

9.135 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.362 ± 0.013

5.016 ± 0.018

5.25 ± 0.031

5.357 ± 0.031

5.311 ± 0.02

8.181 ± 0.029

5.908 ± 0.025

5.685 ± 0.017

0.999 ± 0.008

3.032 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski