Streptococcus satellite phage Javan228

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZGU8|A0A4D5ZGU8_9VIRU Addiction module toxin OS=Streptococcus satellite phage Javan228 OX=2558581 GN=JavanS228_0015 PE=4 SV=1
MM1 pKa = 7.9IYY3 pKa = 10.24QEE5 pKa = 4.11INLPIWAQLVLIVVLILVLLLLAYY29 pKa = 9.32IKK31 pKa = 10.34PVEE34 pKa = 4.35DD35 pKa = 3.88VEE37 pKa = 4.38EE38 pKa = 4.52EE39 pKa = 3.79NDD41 pKa = 3.64KK42 pKa = 10.92NHH44 pKa = 7.22PIIMM48 pKa = 4.72

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZLC4|A0A4D5ZLC4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan228 OX=2558581 GN=JavanS228_0013 PE=4 SV=1
MM1 pKa = 7.91IITGIQARR9 pKa = 11.84AVRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 9.48QADD17 pKa = 3.22NMLTAKK23 pKa = 9.41ATAQAIGISPITYY36 pKa = 9.42KK37 pKa = 10.74KK38 pKa = 9.81VVQGGKK44 pKa = 9.68VKK46 pKa = 9.15NTVYY50 pKa = 10.58VKK52 pKa = 10.48VMNWLVEE59 pKa = 4.53DD60 pKa = 4.09YY61 pKa = 11.41

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

2137

39

467

142.5

16.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.756 ± 0.603

0.374 ± 0.153

5.662 ± 0.594

7.815 ± 0.877

4.352 ± 0.723

3.884 ± 0.448

1.17 ± 0.235

7.394 ± 0.602

9.499 ± 0.725

9.733 ± 0.729

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.387

6.692 ± 0.445

2.153 ± 0.216

4.445 ± 0.438

4.352 ± 0.415

5.054 ± 0.57

6.317 ± 0.623

6.551 ± 0.712

1.217 ± 0.188

5.147 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski