Salpingoeca rosetta (strain ATCC 50818 / BSB-021)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Choanoflagellata; Craspedida; Salpingoecidae; Salpingoeca

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11698 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2U247|F2U247_SALR5 Uncharacterized protein OS=Salpingoeca rosetta (strain ATCC 50818 / BSB-021) OX=946362 GN=PTSG_02412 PE=4 SV=1
MM1 pKa = 7.75KK2 pKa = 9.56LTFALVAAAAILACATAQDD21 pKa = 3.99IFGVWTGPVSPGSDD35 pKa = 3.16CSILGEE41 pKa = 3.97FRR43 pKa = 11.84EE44 pKa = 4.53TSFFTIISGEE54 pKa = 4.08EE55 pKa = 3.83SSGCASGIFIGSLTVTSPASGSSADD80 pKa = 3.42TSIIITYY87 pKa = 9.92VEE89 pKa = 4.37VPDD92 pKa = 4.57FATDD96 pKa = 3.51QISPGDD102 pKa = 3.56TFVFDD107 pKa = 4.65IYY109 pKa = 11.15LSGDD113 pKa = 3.22TMTLRR118 pKa = 11.84NDD120 pKa = 3.37YY121 pKa = 11.1FEE123 pKa = 4.68TQGLWPTGGLVLTTYY138 pKa = 9.1PAWPINGYY146 pKa = 9.13WDD148 pKa = 4.23GNDD151 pKa = 3.24TSLLYY156 pKa = 10.46INNGLYY162 pKa = 10.57LRR164 pKa = 11.84ASAFGEE170 pKa = 4.3GGEE173 pKa = 4.34GSSAGVVEE181 pKa = 4.39MTGNTDD187 pKa = 2.86EE188 pKa = 4.18YY189 pKa = 9.52MSRR192 pKa = 11.84IVGSVSDD199 pKa = 4.4IEE201 pKa = 4.95GDD203 pKa = 3.53TYY205 pKa = 11.19TFTMPSSGDD214 pKa = 3.13TRR216 pKa = 11.84LLDD219 pKa = 4.17FSDD222 pKa = 4.05PADD225 pKa = 3.43MNGFLLTKK233 pKa = 10.73NEE235 pKa = 4.02DD236 pKa = 3.53DD237 pKa = 4.21EE238 pKa = 5.62FNGIYY243 pKa = 10.44YY244 pKa = 10.2GVSPAQGEE252 pKa = 4.53DD253 pKa = 3.55CMQSTDD259 pKa = 3.83FNNGIFRR266 pKa = 11.84AFRR269 pKa = 11.84FQCDD273 pKa = 3.69DD274 pKa = 4.16SISDD278 pKa = 3.55DD279 pKa = 3.65GLRR282 pKa = 11.84VSAYY286 pKa = 8.27IGRR289 pKa = 11.84YY290 pKa = 5.28TVSGNQMRR298 pKa = 11.84LLYY301 pKa = 10.07TYY303 pKa = 9.98AAPDD307 pKa = 3.95DD308 pKa = 4.58DD309 pKa = 4.58LAGAEE314 pKa = 4.44VVFTYY319 pKa = 9.71TRR321 pKa = 11.84SGRR324 pKa = 11.84QLTLTGSVGGSDD336 pKa = 3.51EE337 pKa = 4.17FTITMNRR344 pKa = 11.84ADD346 pKa = 4.72RR347 pKa = 11.84APAATVVNITVSGDD361 pKa = 3.31VNTFDD366 pKa = 5.44ADD368 pKa = 3.31AFLAGLAQVLDD379 pKa = 3.7IPVNTIEE386 pKa = 4.54VVSISPGSVVVVVAIRR402 pKa = 11.84EE403 pKa = 4.15DD404 pKa = 3.81ASDD407 pKa = 3.39SSAPSLSTIIKK418 pKa = 10.03RR419 pKa = 11.84IEE421 pKa = 3.95AVPAGTSIGGYY432 pKa = 8.85GLEE435 pKa = 4.2SAQASRR441 pKa = 11.84DD442 pKa = 3.82PGSTSAASVATTSWFAAAILFMAVVSQLLAA472 pKa = 4.53

Molecular weight: 49.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2UC10|F2UC10_SALR5 Gyltl1b protein OS=Salpingoeca rosetta (strain ATCC 50818 / BSB-021) OX=946362 GN=PTSG_06127 PE=4 SV=1
MM1 pKa = 6.93TMRR4 pKa = 11.84MRR6 pKa = 11.84RR7 pKa = 11.84MTFGTLVCAVAMALALASAGCAAGKK32 pKa = 10.02NGRR35 pKa = 11.84KK36 pKa = 9.17RR37 pKa = 11.84RR38 pKa = 11.84LLAFFGLVACKK49 pKa = 9.22QRR51 pKa = 11.84KK52 pKa = 6.33QRR54 pKa = 11.84KK55 pKa = 5.94QRR57 pKa = 11.84KK58 pKa = 5.93QRR60 pKa = 11.84KK61 pKa = 5.93QRR63 pKa = 11.84KK64 pKa = 5.93QRR66 pKa = 11.84KK67 pKa = 5.93QRR69 pKa = 11.84KK70 pKa = 5.93QRR72 pKa = 11.84KK73 pKa = 5.93QRR75 pKa = 11.84KK76 pKa = 5.93QRR78 pKa = 11.84KK79 pKa = 5.93QRR81 pKa = 11.84KK82 pKa = 5.93QRR84 pKa = 11.84KK85 pKa = 5.93QRR87 pKa = 11.84KK88 pKa = 5.93QRR90 pKa = 11.84KK91 pKa = 5.93QRR93 pKa = 11.84KK94 pKa = 5.93QRR96 pKa = 11.84KK97 pKa = 5.93QRR99 pKa = 11.84KK100 pKa = 5.93QRR102 pKa = 11.84KK103 pKa = 5.93QRR105 pKa = 11.84KK106 pKa = 5.93QRR108 pKa = 11.84KK109 pKa = 5.93QRR111 pKa = 11.84KK112 pKa = 5.93QRR114 pKa = 11.84KK115 pKa = 5.93QRR117 pKa = 11.84KK118 pKa = 5.93QRR120 pKa = 11.84KK121 pKa = 5.93QRR123 pKa = 11.84KK124 pKa = 5.93QRR126 pKa = 11.84KK127 pKa = 5.93QRR129 pKa = 11.84KK130 pKa = 5.93QRR132 pKa = 11.84KK133 pKa = 5.93QRR135 pKa = 11.84KK136 pKa = 6.88QRR138 pKa = 11.84KK139 pKa = 5.96QCKK142 pKa = 6.87QHH144 pKa = 5.83

Molecular weight: 18.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11614

84

11698

7789328

50

12309

665.9

72.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.363 ± 0.028

1.727 ± 0.02

6.517 ± 0.021

5.612 ± 0.03

3.082 ± 0.012

6.796 ± 0.026

3.185 ± 0.018

3.279 ± 0.015

3.879 ± 0.028

8.333 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.093 ± 0.011

3.158 ± 0.012

5.266 ± 0.022

5.444 ± 0.033

6.484 ± 0.024

7.917 ± 0.025

7.069 ± 0.032

6.763 ± 0.031

1.012 ± 0.007

2.019 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski