Amino acid dipepetide frequency for Bacillus phage vB_BmeM-Goe8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.021AlaAla: 5.021 ± 0.41
0.388AlaCys: 0.388 ± 0.093
4.288AlaAsp: 4.288 ± 0.293
4.978AlaGlu: 4.978 ± 0.352
2.888AlaPhe: 2.888 ± 0.243
4.439AlaGly: 4.439 ± 0.378
0.948AlaHis: 0.948 ± 0.125
4.568AlaIle: 4.568 ± 0.315
5.517AlaLys: 5.517 ± 0.402
5.366AlaLeu: 5.366 ± 0.288
1.746AlaMet: 1.746 ± 0.17
3.599AlaAsn: 3.599 ± 0.266
2.521AlaPro: 2.521 ± 0.239
2.801AlaGln: 2.801 ± 0.315
2.414AlaArg: 2.414 ± 0.199
3.857AlaSer: 3.857 ± 0.245
4.288AlaThr: 4.288 ± 0.369
4.439AlaVal: 4.439 ± 0.324
0.625AlaTrp: 0.625 ± 0.122
2.478AlaTyr: 2.478 ± 0.214
0.0AlaXaa: 0.0 ± 0.0
Cys
0.431CysAla: 0.431 ± 0.092
0.065CysCys: 0.065 ± 0.036
0.474CysAsp: 0.474 ± 0.124
0.366CysGlu: 0.366 ± 0.088
0.151CysPhe: 0.151 ± 0.05
0.56CysGly: 0.56 ± 0.145
0.237CysHis: 0.237 ± 0.073
0.453CysIle: 0.453 ± 0.107
0.797CysLys: 0.797 ± 0.158
0.431CysLeu: 0.431 ± 0.1
0.172CysMet: 0.172 ± 0.066
0.388CysAsn: 0.388 ± 0.095
0.366CysPro: 0.366 ± 0.086
0.151CysGln: 0.151 ± 0.055
0.431CysArg: 0.431 ± 0.109
0.431CysSer: 0.431 ± 0.092
0.539CysThr: 0.539 ± 0.111
0.409CysVal: 0.409 ± 0.1
0.086CysTrp: 0.086 ± 0.044
0.215CysTyr: 0.215 ± 0.062
0.0CysXaa: 0.0 ± 0.0
Asp
3.405AspAla: 3.405 ± 0.256
0.517AspCys: 0.517 ± 0.12
2.888AspAsp: 2.888 ± 0.308
4.267AspGlu: 4.267 ± 0.333
3.017AspPhe: 3.017 ± 0.286
3.728AspGly: 3.728 ± 0.325
0.991AspHis: 0.991 ± 0.148
4.762AspIle: 4.762 ± 0.338
4.482AspLys: 4.482 ± 0.326
4.956AspLeu: 4.956 ± 0.32
1.853AspMet: 1.853 ± 0.208
3.254AspAsn: 3.254 ± 0.279
2.004AspPro: 2.004 ± 0.227
1.961AspGln: 1.961 ± 0.193
2.5AspArg: 2.5 ± 0.25
3.836AspSer: 3.836 ± 0.319
4.094AspThr: 4.094 ± 0.348
4.051AspVal: 4.051 ± 0.314
0.927AspTrp: 0.927 ± 0.15
3.297AspTyr: 3.297 ± 0.253
0.0AspXaa: 0.0 ± 0.0
Glu
5.452GluAla: 5.452 ± 0.396
0.603GluCys: 0.603 ± 0.11
4.978GluAsp: 4.978 ± 0.335
8.749GluGlu: 8.749 ± 0.709
3.125GluPhe: 3.125 ± 0.302
4.87GluGly: 4.87 ± 0.403
1.832GluHis: 1.832 ± 0.21
4.698GluIle: 4.698 ± 0.385
6.249GluLys: 6.249 ± 0.454
6.982GluLeu: 6.982 ± 0.443
2.414GluMet: 2.414 ± 0.235
3.448GluAsn: 3.448 ± 0.303
2.414GluPro: 2.414 ± 0.347
3.276GluGln: 3.276 ± 0.334
3.319GluArg: 3.319 ± 0.359
3.491GluSer: 3.491 ± 0.249
3.383GluThr: 3.383 ± 0.245
5.215GluVal: 5.215 ± 0.289
0.927GluTrp: 0.927 ± 0.146
3.017GluTyr: 3.017 ± 0.323
0.0GluXaa: 0.0 ± 0.0
Phe
2.457PheAla: 2.457 ± 0.253
0.302PheCys: 0.302 ± 0.088
2.823PheAsp: 2.823 ± 0.225
2.521PheGlu: 2.521 ± 0.218
1.659PhePhe: 1.659 ± 0.19
2.694PheGly: 2.694 ± 0.251
0.862PheHis: 0.862 ± 0.154
2.866PheIle: 2.866 ± 0.221
2.801PheLys: 2.801 ± 0.259
2.823PheLeu: 2.823 ± 0.25
1.056PheMet: 1.056 ± 0.147
2.37PheAsn: 2.37 ± 0.202
1.293PhePro: 1.293 ± 0.155
1.508PheGln: 1.508 ± 0.171
1.616PheArg: 1.616 ± 0.181
3.082PheSer: 3.082 ± 0.288
3.017PheThr: 3.017 ± 0.306
2.37PheVal: 2.37 ± 0.281
0.409PheTrp: 0.409 ± 0.111
1.638PheTyr: 1.638 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
4.245GlyAla: 4.245 ± 0.38
0.56GlyCys: 0.56 ± 0.104
3.922GlyAsp: 3.922 ± 0.314
4.504GlyGlu: 4.504 ± 0.343
2.78GlyPhe: 2.78 ± 0.253
5.754GlyGly: 5.754 ± 0.696
1.185GlyHis: 1.185 ± 0.172
4.137GlyIle: 4.137 ± 0.305
5.193GlyLys: 5.193 ± 0.365
4.956GlyLeu: 4.956 ± 0.373
1.918GlyMet: 1.918 ± 0.177
3.965GlyAsn: 3.965 ± 0.282
0.927GlyPro: 0.927 ± 0.141
2.198GlyGln: 2.198 ± 0.285
2.607GlyArg: 2.607 ± 0.197
4.094GlySer: 4.094 ± 0.338
4.612GlyThr: 4.612 ± 0.365
4.633GlyVal: 4.633 ± 0.359
0.905GlyTrp: 0.905 ± 0.163
2.888GlyTyr: 2.888 ± 0.256
0.0GlyXaa: 0.0 ± 0.0
His
0.905HisAla: 0.905 ± 0.122
0.215HisCys: 0.215 ± 0.069
0.776HisAsp: 0.776 ± 0.141
0.97HisGlu: 0.97 ± 0.16
0.776HisPhe: 0.776 ± 0.165
1.121HisGly: 1.121 ± 0.159
0.56HisHis: 0.56 ± 0.122
1.465HisIle: 1.465 ± 0.2
1.465HisLys: 1.465 ± 0.202
1.53HisLeu: 1.53 ± 0.226
0.474HisMet: 0.474 ± 0.099
1.099HisAsn: 1.099 ± 0.15
0.582HisPro: 0.582 ± 0.1
0.56HisGln: 0.56 ± 0.1
1.034HisArg: 1.034 ± 0.171
0.884HisSer: 0.884 ± 0.135
1.185HisThr: 1.185 ± 0.139
1.293HisVal: 1.293 ± 0.197
0.345HisTrp: 0.345 ± 0.094
0.862HisTyr: 0.862 ± 0.165
0.0HisXaa: 0.0 ± 0.0
Ile
4.87IleAla: 4.87 ± 0.349
0.539IleCys: 0.539 ± 0.112
4.806IleAsp: 4.806 ± 0.326
5.581IleGlu: 5.581 ± 0.395
2.198IlePhe: 2.198 ± 0.213
4.116IleGly: 4.116 ± 0.324
1.077IleHis: 1.077 ± 0.168
3.857IleIle: 3.857 ± 0.36
5.215IleLys: 5.215 ± 0.304
4.375IleLeu: 4.375 ± 0.394
1.336IleMet: 1.336 ± 0.178
4.116IleAsn: 4.116 ± 0.321
2.715IlePro: 2.715 ± 0.26
2.435IleGln: 2.435 ± 0.196
3.06IleArg: 3.06 ± 0.261
3.706IleSer: 3.706 ± 0.337
4.008IleThr: 4.008 ± 0.367
3.987IleVal: 3.987 ± 0.301
0.366IleTrp: 0.366 ± 0.09
2.198IleTyr: 2.198 ± 0.228
0.0IleXaa: 0.0 ± 0.0
Lys
5.624LysAla: 5.624 ± 0.389
0.582LysCys: 0.582 ± 0.152
4.568LysAsp: 4.568 ± 0.327
7.219LysGlu: 7.219 ± 0.496
2.952LysPhe: 2.952 ± 0.275
5.15LysGly: 5.15 ± 0.35
1.552LysHis: 1.552 ± 0.202
3.9LysIle: 3.9 ± 0.32
6.616LysLys: 6.616 ± 0.559
6.098LysLeu: 6.098 ± 0.369
2.651LysMet: 2.651 ± 0.219
3.469LysAsn: 3.469 ± 0.266
2.478LysPro: 2.478 ± 0.237
3.276LysGln: 3.276 ± 0.262
3.491LysArg: 3.491 ± 0.315
4.116LysSer: 4.116 ± 0.327
3.836LysThr: 3.836 ± 0.295
5.43LysVal: 5.43 ± 0.342
0.84LysTrp: 0.84 ± 0.129
3.362LysTyr: 3.362 ± 0.26
0.0LysXaa: 0.0 ± 0.0
Leu
5.237LeuAla: 5.237 ± 0.32
0.517LeuCys: 0.517 ± 0.119
5.15LeuAsp: 5.15 ± 0.4
6.206LeuGlu: 6.206 ± 0.35
2.478LeuPhe: 2.478 ± 0.203
4.267LeuGly: 4.267 ± 0.313
1.358LeuHis: 1.358 ± 0.182
4.461LeuIle: 4.461 ± 0.346
5.754LeuLys: 5.754 ± 0.288
5.366LeuLeu: 5.366 ± 0.36
1.832LeuMet: 1.832 ± 0.187
4.353LeuAsn: 4.353 ± 0.363
2.952LeuPro: 2.952 ± 0.267
3.34LeuGln: 3.34 ± 0.275
4.073LeuArg: 4.073 ± 0.344
6.055LeuSer: 6.055 ± 0.351
5.452LeuThr: 5.452 ± 0.394
4.913LeuVal: 4.913 ± 0.297
0.711LeuTrp: 0.711 ± 0.116
3.038LeuTyr: 3.038 ± 0.241
0.0LeuXaa: 0.0 ± 0.0
Met
2.349MetAla: 2.349 ± 0.208
0.28MetCys: 0.28 ± 0.074
1.746MetAsp: 1.746 ± 0.177
2.543MetGlu: 2.543 ± 0.26
1.077MetPhe: 1.077 ± 0.17
1.207MetGly: 1.207 ± 0.153
0.409MetHis: 0.409 ± 0.094
1.616MetIle: 1.616 ± 0.222
2.263MetLys: 2.263 ± 0.249
2.069MetLeu: 2.069 ± 0.214
0.646MetMet: 0.646 ± 0.106
1.422MetAsn: 1.422 ± 0.202
0.776MetPro: 0.776 ± 0.117
0.97MetGln: 0.97 ± 0.152
1.142MetArg: 1.142 ± 0.15
2.004MetSer: 2.004 ± 0.225
1.616MetThr: 1.616 ± 0.185
1.336MetVal: 1.336 ± 0.159
0.151MetTrp: 0.151 ± 0.059
1.25MetTyr: 1.25 ± 0.138
0.0MetXaa: 0.0 ± 0.0
Asn
3.232AsnAla: 3.232 ± 0.279
0.409AsnCys: 0.409 ± 0.094
2.607AsnAsp: 2.607 ± 0.25
3.276AsnGlu: 3.276 ± 0.249
2.284AsnPhe: 2.284 ± 0.222
4.181AsnGly: 4.181 ± 0.344
0.884AsnHis: 0.884 ± 0.139
3.771AsnIle: 3.771 ± 0.308
4.396AsnLys: 4.396 ± 0.296
4.094AsnLeu: 4.094 ± 0.282
1.595AsnMet: 1.595 ± 0.198
3.038AsnAsn: 3.038 ± 0.313
2.866AsnPro: 2.866 ± 0.293
1.983AsnGln: 1.983 ± 0.231
2.392AsnArg: 2.392 ± 0.231
3.383AsnSer: 3.383 ± 0.319
3.599AsnThr: 3.599 ± 0.362
3.922AsnVal: 3.922 ± 0.376
0.345AsnTrp: 0.345 ± 0.077
2.241AsnTyr: 2.241 ± 0.204
0.0AsnXaa: 0.0 ± 0.0
Pro
2.521ProAla: 2.521 ± 0.285
0.086ProCys: 0.086 ± 0.038
2.198ProAsp: 2.198 ± 0.217
3.426ProGlu: 3.426 ± 0.373
1.422ProPhe: 1.422 ± 0.22
1.444ProGly: 1.444 ± 0.177
0.646ProHis: 0.646 ± 0.132
2.133ProIle: 2.133 ± 0.213
2.651ProLys: 2.651 ± 0.26
2.521ProLeu: 2.521 ± 0.241
0.646ProMet: 0.646 ± 0.117
2.047ProAsn: 2.047 ± 0.202
0.884ProPro: 0.884 ± 0.175
1.077ProGln: 1.077 ± 0.161
1.207ProArg: 1.207 ± 0.141
1.616ProSer: 1.616 ± 0.23
2.909ProThr: 2.909 ± 0.273
2.284ProVal: 2.284 ± 0.256
0.215ProTrp: 0.215 ± 0.069
1.573ProTyr: 1.573 ± 0.197
0.0ProXaa: 0.0 ± 0.0
Gln
3.038GlnAla: 3.038 ± 0.314
0.108GlnCys: 0.108 ± 0.049
1.918GlnAsp: 1.918 ± 0.198
2.845GlnGlu: 2.845 ± 0.245
1.099GlnPhe: 1.099 ± 0.14
2.478GlnGly: 2.478 ± 0.258
0.711GlnHis: 0.711 ± 0.161
2.09GlnIle: 2.09 ± 0.188
2.435GlnLys: 2.435 ± 0.228
3.491GlnLeu: 3.491 ± 0.279
1.271GlnMet: 1.271 ± 0.168
2.241GlnAsn: 2.241 ± 0.211
1.271GlnPro: 1.271 ± 0.2
2.306GlnGln: 2.306 ± 0.686
1.638GlnArg: 1.638 ± 0.175
2.521GlnSer: 2.521 ± 0.274
2.026GlnThr: 2.026 ± 0.206
2.995GlnVal: 2.995 ± 0.251
0.517GlnTrp: 0.517 ± 0.104
1.401GlnTyr: 1.401 ± 0.136
0.0GlnXaa: 0.0 ± 0.0
Arg
2.392ArgAla: 2.392 ± 0.206
0.28ArgCys: 0.28 ± 0.084
2.629ArgAsp: 2.629 ± 0.24
3.793ArgGlu: 3.793 ± 0.324
1.789ArgPhe: 1.789 ± 0.208
3.168ArgGly: 3.168 ± 0.278
0.625ArgHis: 0.625 ± 0.12
3.491ArgIle: 3.491 ± 0.244
3.642ArgLys: 3.642 ± 0.34
3.663ArgLeu: 3.663 ± 0.279
1.25ArgMet: 1.25 ± 0.17
2.026ArgAsn: 2.026 ± 0.175
0.948ArgPro: 0.948 ± 0.139
1.616ArgGln: 1.616 ± 0.176
1.939ArgArg: 1.939 ± 0.262
2.198ArgSer: 2.198 ± 0.222
2.564ArgThr: 2.564 ± 0.258
2.823ArgVal: 2.823 ± 0.267
0.474ArgTrp: 0.474 ± 0.107
2.263ArgTyr: 2.263 ± 0.174
0.0ArgXaa: 0.0 ± 0.0
Ser
3.577SerAla: 3.577 ± 0.302
0.345SerCys: 0.345 ± 0.087
3.211SerAsp: 3.211 ± 0.312
3.922SerGlu: 3.922 ± 0.318
2.651SerPhe: 2.651 ± 0.227
4.784SerGly: 4.784 ± 0.396
1.185SerHis: 1.185 ± 0.197
4.202SerIle: 4.202 ± 0.308
3.793SerLys: 3.793 ± 0.312
4.999SerLeu: 4.999 ± 0.319
1.746SerMet: 1.746 ± 0.185
3.405SerAsn: 3.405 ± 0.294
1.789SerPro: 1.789 ± 0.2
2.069SerGln: 2.069 ± 0.215
2.478SerArg: 2.478 ± 0.253
4.159SerSer: 4.159 ± 0.386
4.03SerThr: 4.03 ± 0.349
4.59SerVal: 4.59 ± 0.355
0.625SerTrp: 0.625 ± 0.142
2.866SerTyr: 2.866 ± 0.277
0.0SerXaa: 0.0 ± 0.0
Thr
4.568ThrAla: 4.568 ± 0.445
0.431ThrCys: 0.431 ± 0.084
3.663ThrAsp: 3.663 ± 0.299
4.396ThrGlu: 4.396 ± 0.315
3.038ThrPhe: 3.038 ± 0.244
4.504ThrGly: 4.504 ± 0.446
0.884ThrHis: 0.884 ± 0.148
4.568ThrIle: 4.568 ± 0.295
4.267ThrLys: 4.267 ± 0.29
5.086ThrLeu: 5.086 ± 0.393
1.228ThrMet: 1.228 ± 0.16
3.297ThrAsn: 3.297 ± 0.259
2.974ThrPro: 2.974 ± 0.223
2.607ThrGln: 2.607 ± 0.264
2.607ThrArg: 2.607 ± 0.246
3.879ThrSer: 3.879 ± 0.416
3.987ThrThr: 3.987 ± 0.48
5.409ThrVal: 5.409 ± 0.477
0.646ThrTrp: 0.646 ± 0.145
2.737ThrTyr: 2.737 ± 0.243
0.0ThrXaa: 0.0 ± 0.0
Val
4.461ValAla: 4.461 ± 0.302
0.539ValCys: 0.539 ± 0.13
4.568ValAsp: 4.568 ± 0.297
5.064ValGlu: 5.064 ± 0.323
2.866ValPhe: 2.866 ± 0.261
4.094ValGly: 4.094 ± 0.295
1.293ValHis: 1.293 ± 0.162
4.224ValIle: 4.224 ± 0.248
5.905ValLys: 5.905 ± 0.325
4.31ValLeu: 4.31 ± 0.271
1.401ValMet: 1.401 ± 0.21
3.879ValAsn: 3.879 ± 0.297
2.715ValPro: 2.715 ± 0.263
2.414ValGln: 2.414 ± 0.213
2.952ValArg: 2.952 ± 0.251
3.944ValSer: 3.944 ± 0.299
5.754ValThr: 5.754 ± 0.454
4.59ValVal: 4.59 ± 0.287
0.56ValTrp: 0.56 ± 0.108
2.801ValTyr: 2.801 ± 0.283
0.0ValXaa: 0.0 ± 0.0
Trp
0.646TrpAla: 0.646 ± 0.115
0.065TrpCys: 0.065 ± 0.04
0.84TrpAsp: 0.84 ± 0.166
0.862TrpGlu: 0.862 ± 0.115
0.453TrpPhe: 0.453 ± 0.128
0.474TrpGly: 0.474 ± 0.111
0.108TrpHis: 0.108 ± 0.05
0.474TrpIle: 0.474 ± 0.11
0.819TrpLys: 0.819 ± 0.145
0.884TrpLeu: 0.884 ± 0.151
0.302TrpMet: 0.302 ± 0.073
0.582TrpAsn: 0.582 ± 0.111
0.0TrpPro: 0.0 ± 0.0
0.474TrpGln: 0.474 ± 0.094
0.215TrpArg: 0.215 ± 0.064
0.625TrpSer: 0.625 ± 0.115
0.797TrpThr: 0.797 ± 0.173
0.819TrpVal: 0.819 ± 0.133
0.194TrpTrp: 0.194 ± 0.072
0.603TrpTyr: 0.603 ± 0.127
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.737TyrAla: 2.737 ± 0.229
0.323TyrCys: 0.323 ± 0.075
2.737TyrAsp: 2.737 ± 0.214
2.952TyrGlu: 2.952 ± 0.248
1.465TyrPhe: 1.465 ± 0.21
2.952TyrGly: 2.952 ± 0.269
0.754TyrHis: 0.754 ± 0.137
2.823TyrIle: 2.823 ± 0.219
2.974TyrLys: 2.974 ± 0.263
3.513TyrLeu: 3.513 ± 0.239
1.315TyrMet: 1.315 ± 0.18
2.5TyrAsn: 2.5 ± 0.23
1.034TyrPro: 1.034 ± 0.149
1.379TyrGln: 1.379 ± 0.159
2.457TyrArg: 2.457 ± 0.245
2.543TyrSer: 2.543 ± 0.244
3.082TyrThr: 3.082 ± 0.301
2.823TyrVal: 2.823 ± 0.278
0.366TyrTrp: 0.366 ± 0.086
1.552TyrTyr: 1.552 ± 0.175
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 227 proteins (46406 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski