Drosophila ananassae (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19363 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3MYL3|B3MYL3_DROAN Uncharacterized protein OS=Drosophila ananassae OX=7217 GN=Dana\GF22009 PE=4 SV=1
MM1 pKa = 7.23IAEE4 pKa = 4.21ALEE7 pKa = 3.93FLFDD11 pKa = 3.87RR12 pKa = 11.84LPYY15 pKa = 10.24EE16 pKa = 4.24SEE18 pKa = 4.04EE19 pKa = 5.04DD20 pKa = 3.32ICDD23 pKa = 3.64MPEE26 pKa = 3.88SLLVLACVLGVLIFVTYY43 pKa = 10.56ILIRR47 pKa = 11.84TDD49 pKa = 2.94ADD51 pKa = 3.29IYY53 pKa = 9.84PLYY56 pKa = 10.39PEE58 pKa = 4.04VHH60 pKa = 6.99PDD62 pKa = 3.66DD63 pKa = 5.03EE64 pKa = 4.54EE65 pKa = 6.94LIDD68 pKa = 5.06DD69 pKa = 5.17LARR72 pKa = 11.84GLNQNLNNWLINGRR86 pKa = 11.84GLAPNDD92 pKa = 3.89RR93 pKa = 11.84NLQQ96 pKa = 3.56

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P9C355|A0A0P9C355_DROAN Uncharacterized protein isoform B OS=Drosophila ananassae OX=7217 GN=Dana\GF10107 PE=4 SV=1
MM1 pKa = 7.2KK2 pKa = 10.08FVWAIVGLLFCLTLDD17 pKa = 3.97QANGTPGEE25 pKa = 4.32SGIKK29 pKa = 9.3PPVWNLPTPRR39 pKa = 11.84RR40 pKa = 11.84TPPRR44 pKa = 11.84PPTPVGLPIRR54 pKa = 11.84RR55 pKa = 11.84PPISPPRR62 pKa = 11.84APVMWRR68 pKa = 11.84PFPRR72 pKa = 11.84PAPRR76 pKa = 11.84TPPPIRR82 pKa = 11.84RR83 pKa = 11.84PPVTRR88 pKa = 11.84KK89 pKa = 9.43PPPRR93 pKa = 11.84NPPPTRR99 pKa = 11.84RR100 pKa = 11.84PPITRR105 pKa = 11.84RR106 pKa = 11.84PTQRR110 pKa = 11.84PAPKK114 pKa = 9.62PLIPTTRR121 pKa = 11.84KK122 pKa = 8.95FLIWTRR128 pKa = 11.84IPTRR132 pKa = 11.84IVPTKK137 pKa = 10.05PNIRR141 pKa = 11.84PTLWTRR147 pKa = 11.84RR148 pKa = 11.84PLPTIRR154 pKa = 11.84PTRR157 pKa = 11.84PRR159 pKa = 11.84IRR161 pKa = 11.84PTLWTRR167 pKa = 11.84PPIKK171 pKa = 9.14ITARR175 pKa = 11.84PRR177 pKa = 11.84PTVRR181 pKa = 11.84ATIRR185 pKa = 11.84PTARR189 pKa = 11.84PPSPTRR195 pKa = 11.84VTKK198 pKa = 10.52RR199 pKa = 11.84PQKK202 pKa = 9.91PRR204 pKa = 11.84TGATLWTRR212 pKa = 11.84PPGRR216 pKa = 11.84NPTTRR221 pKa = 11.84QLTFIFEE228 pKa = 4.61TPQPTTQRR236 pKa = 11.84PRR238 pKa = 11.84PRR240 pKa = 11.84EE241 pKa = 3.86PPRR244 pKa = 11.84SGSTRR249 pKa = 11.84SLIFIFTTRR258 pKa = 11.84RR259 pKa = 11.84PNRR262 pKa = 11.84TVHH265 pKa = 5.64PTGHH269 pKa = 5.41TRR271 pKa = 11.84KK272 pKa = 9.97SS273 pKa = 3.21

Molecular weight: 31.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14231

5132

19363

12990802

21

18780

670.9

74.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.394 ± 0.022

1.991 ± 0.067

5.257 ± 0.016

6.674 ± 0.032

3.346 ± 0.016

6.243 ± 0.026

2.57 ± 0.011

4.819 ± 0.017

5.651 ± 0.029

8.795 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.192 ± 0.012

4.708 ± 0.017

5.849 ± 0.038

5.27 ± 0.024

5.535 ± 0.014

8.362 ± 0.029

5.717 ± 0.02

5.857 ± 0.015

0.95 ± 0.007

2.817 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski