Amino acid dipepetide frequency for Drosophila ananassae (Fruit fly)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.892AlaAla: 8.892 ± 0.097	1.296AlaCys: 1.296 ± 0.059	3.323AlaAsp: 3.323 ± 0.019	4.723AlaGlu: 4.723 ± 0.029	2.062AlaPhe: 2.062 ± 0.016	5.006AlaGly: 5.006 ± 0.031	1.584AlaHis: 1.584 ± 0.012	3.335AlaIle: 3.335 ± 0.02	4.116AlaLys: 4.116 ± 0.028	6.069AlaLeu: 6.069 ± 0.038	1.621AlaMet: 1.621 ± 0.013	3.149AlaAsn: 3.149 ± 0.021	4.401AlaPro: 4.401 ± 0.039	3.346AlaGln: 3.346 ± 0.027	3.322AlaArg: 3.322 ± 0.021	6.138AlaSer: 6.138 ± 0.043	4.862AlaThr: 4.862 ± 0.032	4.442AlaVal: 4.442 ± 0.022	0.568AlaTrp: 0.568 ± 0.008	1.687AlaTyr: 1.687 ± 0.013	0.002AlaXaa: 0.002 ± 0.0
Cys	1.196CysAla: 1.196 ± 0.035	0.444CysCys: 0.444 ± 0.008	1.047CysAsp: 1.047 ± 0.019	1.128CysGlu: 1.128 ± 0.023	0.674CysPhe: 0.674 ± 0.01	1.513CysGly: 1.513 ± 0.074	0.511CysHis: 0.511 ± 0.013	1.047CysIle: 1.047 ± 0.037	0.96CysLys: 0.96 ± 0.015	1.779CysLeu: 1.779 ± 0.05	0.354CysMet: 0.354 ± 0.006	0.893CysAsn: 0.893 ± 0.02	1.203CysPro: 1.203 ± 0.068	1.021CysGln: 1.021 ± 0.051	1.416CysArg: 1.416 ± 0.093	1.673CysSer: 1.673 ± 0.054	1.023CysThr: 1.023 ± 0.032	1.293CysVal: 1.293 ± 0.056	0.179CysTrp: 0.179 ± 0.004	0.552CysTyr: 0.552 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	3.61AspAla: 3.61 ± 0.024	1.058AspCys: 1.058 ± 0.032	3.699AspAsp: 3.699 ± 0.034	4.336AspGlu: 4.336 ± 0.029	2.086AspPhe: 2.086 ± 0.015	3.237AspGly: 3.237 ± 0.03	1.097AspHis: 1.097 ± 0.01	2.799AspIle: 2.799 ± 0.023	2.73AspLys: 2.73 ± 0.022	4.724AspLeu: 4.724 ± 0.036	1.203AspMet: 1.203 ± 0.013	2.26AspAsn: 2.26 ± 0.019	2.89AspPro: 2.89 ± 0.058	2.012AspGln: 2.012 ± 0.015	2.606AspArg: 2.606 ± 0.023	4.073AspSer: 4.073 ± 0.026	2.534AspThr: 2.534 ± 0.017	3.314AspVal: 3.314 ± 0.021	0.585AspTrp: 0.585 ± 0.009	1.718AspTyr: 1.718 ± 0.015	0.001AspXaa: 0.001 ± 0.0
Glu	4.786GluAla: 4.786 ± 0.039	1.396GluCys: 1.396 ± 0.079	4.147GluAsp: 4.147 ± 0.029	6.394GluGlu: 6.394 ± 0.072	2.105GluPhe: 2.105 ± 0.018	3.041GluGly: 3.041 ± 0.026	1.539GluHis: 1.539 ± 0.013	3.332GluIle: 3.332 ± 0.029	4.509GluLys: 4.509 ± 0.039	6.074GluLeu: 6.074 ± 0.046	1.492GluMet: 1.492 ± 0.016	3.009GluAsn: 3.009 ± 0.021	3.174GluPro: 3.174 ± 0.036	3.541GluGln: 3.541 ± 0.032	4.08GluArg: 4.08 ± 0.031	4.51GluSer: 4.51 ± 0.03	3.475GluThr: 3.475 ± 0.035	3.77GluVal: 3.77 ± 0.041	0.588GluTrp: 0.588 ± 0.01	1.78GluTyr: 1.78 ± 0.018	0.001GluXaa: 0.001 ± 0.0
Phe	2.096PheAla: 2.096 ± 0.015	0.69PheCys: 0.69 ± 0.009	2.029PheAsp: 2.029 ± 0.016	2.088PheGlu: 2.088 ± 0.016	1.363PhePhe: 1.363 ± 0.014	2.334PheGly: 2.334 ± 0.02	0.855PheHis: 0.855 ± 0.009	1.774PheIle: 1.774 ± 0.017	1.789PheLys: 1.789 ± 0.015	3.143PheLeu: 3.143 ± 0.025	0.801PheMet: 0.801 ± 0.009	1.631PheAsn: 1.631 ± 0.011	1.37PhePro: 1.37 ± 0.012	1.429PheGln: 1.429 ± 0.012	1.899PheArg: 1.899 ± 0.02	2.527PheSer: 2.527 ± 0.02	1.764PheThr: 1.764 ± 0.014	2.302PheVal: 2.302 ± 0.016	0.4PheTrp: 0.4 ± 0.008	1.17PheTyr: 1.17 ± 0.011	0.001PheXaa: 0.001 ± 0.0
Gly	4.661GlyAla: 4.661 ± 0.037	1.099GlyCys: 1.099 ± 0.031	3.228GlyAsp: 3.228 ± 0.03	3.435GlyGlu: 3.435 ± 0.034	2.164GlyPhe: 2.164 ± 0.022	7.04GlyGly: 7.04 ± 0.091	1.638GlyHis: 1.638 ± 0.016	2.794GlyIle: 2.794 ± 0.018	3.152GlyLys: 3.152 ± 0.026	4.677GlyLeu: 4.677 ± 0.026	1.32GlyMet: 1.32 ± 0.014	3.113GlyAsn: 3.113 ± 0.031	2.702GlyPro: 2.702 ± 0.029	2.693GlyGln: 2.693 ± 0.028	3.051GlyArg: 3.051 ± 0.019	6.17GlySer: 6.17 ± 0.043	3.252GlyThr: 3.252 ± 0.024	3.611GlyVal: 3.611 ± 0.024	0.581GlyTrp: 0.581 ± 0.008	2.045GlyTyr: 2.045 ± 0.028	0.001GlyXaa: 0.001 ± 0.0
His	1.47HisAla: 1.47 ± 0.013	0.558HisCys: 0.558 ± 0.012	1.084HisAsp: 1.084 ± 0.01	1.349HisGlu: 1.349 ± 0.011	0.994HisPhe: 0.994 ± 0.01	1.45HisGly: 1.45 ± 0.013	1.46HisHis: 1.46 ± 0.024	1.267HisIle: 1.267 ± 0.012	1.264HisLys: 1.264 ± 0.011	2.469HisLeu: 2.469 ± 0.016	0.652HisMet: 0.652 ± 0.008	1.157HisAsn: 1.157 ± 0.012	1.538HisPro: 1.538 ± 0.016	1.678HisGln: 1.678 ± 0.021	1.432HisArg: 1.432 ± 0.014	2.117HisSer: 2.117 ± 0.017	1.281HisThr: 1.281 ± 0.011	1.394HisVal: 1.394 ± 0.01	0.272HisTrp: 0.272 ± 0.005	0.814HisTyr: 0.814 ± 0.009	0.0HisXaa: 0.0 ± 0.0
Ile	3.206IleAla: 3.206 ± 0.018	1.192IleCys: 1.192 ± 0.035	2.695IleAsp: 2.695 ± 0.025	3.2IleGlu: 3.2 ± 0.033	1.972IlePhe: 1.972 ± 0.017	2.631IleGly: 2.631 ± 0.019	1.069IleHis: 1.069 ± 0.009	2.598IleIle: 2.598 ± 0.026	2.897IleLys: 2.897 ± 0.03	4.225IleLeu: 4.225 ± 0.032	1.04IleMet: 1.04 ± 0.011	2.449IleAsn: 2.449 ± 0.024	2.472IlePro: 2.472 ± 0.018	2.045IleGln: 2.045 ± 0.016	2.491IleArg: 2.491 ± 0.016	4.001IleSer: 4.001 ± 0.024	2.823IleThr: 2.823 ± 0.028	3.115IleVal: 3.115 ± 0.024	0.503IleTrp: 0.503 ± 0.008	1.566IleTyr: 1.566 ± 0.013	0.002IleXaa: 0.002 ± 0.0
Lys	3.691LysAla: 3.691 ± 0.025	1.201LysCys: 1.201 ± 0.033	3.124LysAsp: 3.124 ± 0.033	4.048LysGlu: 4.048 ± 0.031	1.854LysPhe: 1.854 ± 0.016	2.426LysGly: 2.426 ± 0.022	1.336LysHis: 1.336 ± 0.014	2.898LysIle: 2.898 ± 0.026	4.3LysLys: 4.3 ± 0.056	5.224LysLeu: 5.224 ± 0.043	1.348LysMet: 1.348 ± 0.013	2.504LysAsn: 2.504 ± 0.024	3.45LysPro: 3.45 ± 0.061	2.683LysGln: 2.683 ± 0.025	3.586LysArg: 3.586 ± 0.023	4.309LysSer: 4.309 ± 0.034	3.089LysThr: 3.089 ± 0.024	3.13LysVal: 3.13 ± 0.029	0.62LysTrp: 0.62 ± 0.013	1.686LysTyr: 1.686 ± 0.018	0.002LysXaa: 0.002 ± 0.0
Leu	6.453LeuAla: 6.453 ± 0.034	1.605LeuCys: 1.605 ± 0.021	4.834LeuAsp: 4.834 ± 0.039	6.293LeuGlu: 6.293 ± 0.05	2.775LeuPhe: 2.775 ± 0.024	4.721LeuGly: 4.721 ± 0.029	2.358LeuHis: 2.358 ± 0.017	4.12LeuIle: 4.12 ± 0.03	5.387LeuLys: 5.387 ± 0.04	8.415LeuLeu: 8.415 ± 0.061	1.94LeuMet: 1.94 ± 0.016	4.238LeuAsn: 4.238 ± 0.025	5.066LeuPro: 5.066 ± 0.027	5.083LeuGln: 5.083 ± 0.042	5.364LeuArg: 5.364 ± 0.038	6.647LeuSer: 6.647 ± 0.042	4.581LeuThr: 4.581 ± 0.023	4.948LeuVal: 4.948 ± 0.032	0.803LeuTrp: 0.803 ± 0.009	2.317LeuTyr: 2.317 ± 0.019	0.002LeuXaa: 0.002 ± 0.0
Met	1.751MetAla: 1.751 ± 0.016	0.392MetCys: 0.392 ± 0.007	1.302MetAsp: 1.302 ± 0.012	1.63MetGlu: 1.63 ± 0.013	0.733MetPhe: 0.733 ± 0.01	1.363MetGly: 1.363 ± 0.015	0.57MetHis: 0.57 ± 0.007	0.901MetIle: 0.901 ± 0.011	1.156MetLys: 1.156 ± 0.011	1.932MetLeu: 1.932 ± 0.016	0.58MetMet: 0.58 ± 0.008	0.946MetAsn: 0.946 ± 0.011	1.277MetPro: 1.277 ± 0.014	1.186MetGln: 1.186 ± 0.013	1.356MetArg: 1.356 ± 0.011	1.714MetSer: 1.714 ± 0.016	1.097MetThr: 1.097 ± 0.01	1.225MetVal: 1.225 ± 0.011	0.211MetTrp: 0.211 ± 0.005	0.595MetTyr: 0.595 ± 0.008	0.001MetXaa: 0.001 ± 0.0
Asn	3.369AsnAla: 3.369 ± 0.045	0.971AsnCys: 0.971 ± 0.023	2.246AsnAsp: 2.246 ± 0.02	2.857AsnGlu: 2.857 ± 0.021	1.695AsnPhe: 1.695 ± 0.014	3.564AsnGly: 3.564 ± 0.028	1.18AsnHis: 1.18 ± 0.024	2.49AsnIle: 2.49 ± 0.019	2.324AsnLys: 2.324 ± 0.02	4.136AsnLeu: 4.136 ± 0.028	1.078AsnMet: 1.078 ± 0.012	3.028AsnAsn: 3.028 ± 0.036	2.571AsnPro: 2.571 ± 0.049	2.058AsnGln: 2.058 ± 0.018	2.325AsnArg: 2.325 ± 0.014	4.164AsnSer: 4.164 ± 0.03	2.371AsnThr: 2.371 ± 0.016	2.733AsnVal: 2.733 ± 0.019	0.477AsnTrp: 0.477 ± 0.007	1.446AsnTyr: 1.446 ± 0.011	0.001AsnXaa: 0.001 ± 0.0
Pro	4.594ProAla: 4.594 ± 0.037	1.133ProCys: 1.133 ± 0.102	2.579ProAsp: 2.579 ± 0.015	3.896ProGlu: 3.896 ± 0.055	1.661ProPhe: 1.661 ± 0.021	3.581ProGly: 3.581 ± 0.046	1.47ProHis: 1.47 ± 0.015	2.564ProIle: 2.564 ± 0.029	3.287ProLys: 3.287 ± 0.037	4.484ProLeu: 4.484 ± 0.027	1.097ProMet: 1.097 ± 0.013	2.543ProAsn: 2.543 ± 0.037	5.897ProPro: 5.897 ± 0.074	2.968ProGln: 2.968 ± 0.027	2.677ProArg: 2.677 ± 0.02	4.964ProSer: 4.964 ± 0.039	3.71ProThr: 3.71 ± 0.027	3.509ProVal: 3.509 ± 0.028	0.425ProTrp: 0.425 ± 0.007	1.452ProTyr: 1.452 ± 0.018	0.001ProXaa: 0.001 ± 0.0
Gln	3.519GlnAla: 3.519 ± 0.024	0.946GlnCys: 0.946 ± 0.05	2.096GlnAsp: 2.096 ± 0.016	3.14GlnGlu: 3.14 ± 0.026	1.488GlnPhe: 1.488 ± 0.013	2.175GlnGly: 2.175 ± 0.018	1.821GlnHis: 1.821 ± 0.023	2.18GlnIle: 2.18 ± 0.02	2.727GlnLys: 2.727 ± 0.022	5.131GlnLeu: 5.131 ± 0.04	1.222GlnMet: 1.222 ± 0.013	2.159GlnAsn: 2.159 ± 0.017	3.157GlnPro: 3.157 ± 0.036	7.397GlnGln: 7.397 ± 0.132	3.3GlnArg: 3.3 ± 0.028	3.51GlnSer: 3.51 ± 0.026	2.493GlnThr: 2.493 ± 0.021	2.568GlnVal: 2.568 ± 0.02	0.435GlnTrp: 0.435 ± 0.006	1.232GlnTyr: 1.232 ± 0.013	0.001GlnXaa: 0.001 ± 0.0
Arg	3.307ArgAla: 3.307 ± 0.017	1.13ArgCys: 1.13 ± 0.033	2.975ArgAsp: 2.975 ± 0.023	3.754ArgGlu: 3.754 ± 0.033	1.872ArgPhe: 1.872 ± 0.014	2.792ArgGly: 2.792 ± 0.021	1.546ArgHis: 1.546 ± 0.012	2.697ArgIle: 2.697 ± 0.016	3.543ArgLys: 3.543 ± 0.025	5.057ArgLeu: 5.057 ± 0.034	1.175ArgMet: 1.175 ± 0.012	2.727ArgAsn: 2.727 ± 0.019	2.957ArgPro: 2.957 ± 0.039	2.978ArgGln: 2.978 ± 0.026	4.463ArgArg: 4.463 ± 0.036	4.527ArgSer: 4.527 ± 0.036	2.747ArgThr: 2.747 ± 0.019	2.924ArgVal: 2.924 ± 0.023	0.561ArgTrp: 0.561 ± 0.008	1.618ArgTyr: 1.618 ± 0.013	0.002ArgXaa: 0.002 ± 0.0
Ser	5.938SerAla: 5.938 ± 0.043	1.605SerCys: 1.605 ± 0.057	4.173SerAsp: 4.173 ± 0.026	4.69SerGlu: 4.69 ± 0.029	2.577SerPhe: 2.577 ± 0.02	6.166SerGly: 6.166 ± 0.043	1.971SerHis: 1.971 ± 0.017	3.684SerIle: 3.684 ± 0.025	4.15SerLys: 4.15 ± 0.031	6.817SerLeu: 6.817 ± 0.041	1.722SerMet: 1.722 ± 0.017	4.181SerAsn: 4.181 ± 0.029	5.075SerPro: 5.075 ± 0.043	3.724SerGln: 3.724 ± 0.026	4.115SerArg: 4.115 ± 0.03	10.278SerSer: 10.278 ± 0.096	5.359SerThr: 5.359 ± 0.047	4.556SerVal: 4.556 ± 0.025	0.74SerTrp: 0.74 ± 0.009	2.098SerTyr: 2.098 ± 0.017	0.003SerXaa: 0.003 ± 0.0
Thr	4.487ThrAla: 4.487 ± 0.029	1.097ThrCys: 1.097 ± 0.03	2.651ThrAsp: 2.651 ± 0.018	3.389ThrGlu: 3.389 ± 0.03	1.779ThrPhe: 1.779 ± 0.014	3.594ThrGly: 3.594 ± 0.031	1.289ThrHis: 1.289 ± 0.011	2.842ThrIle: 2.842 ± 0.022	2.935ThrLys: 2.935 ± 0.026	4.833ThrLeu: 4.833 ± 0.028	1.083ThrMet: 1.083 ± 0.009	2.552ThrAsn: 2.552 ± 0.016	4.149ThrPro: 4.149 ± 0.032	2.298ThrGln: 2.298 ± 0.017	2.553ThrArg: 2.553 ± 0.022	5.06ThrSer: 5.06 ± 0.037	5.145ThrThr: 5.145 ± 0.075	3.475ThrVal: 3.475 ± 0.031	0.513ThrTrp: 0.513 ± 0.01	1.438ThrTyr: 1.438 ± 0.011	0.002ThrXaa: 0.002 ± 0.0
Val	4.579ValAla: 4.579 ± 0.024	1.259ValCys: 1.259 ± 0.041	3.211ValAsp: 3.211 ± 0.024	4.082ValGlu: 4.082 ± 0.052	2.024ValPhe: 2.024 ± 0.015	3.481ValGly: 3.481 ± 0.024	1.391ValHis: 1.391 ± 0.012	2.938ValIle: 2.938 ± 0.022	3.206ValLys: 3.206 ± 0.034	5.183ValLeu: 5.183 ± 0.03	1.238ValMet: 1.238 ± 0.011	2.663ValAsn: 2.663 ± 0.021	3.437ValPro: 3.437 ± 0.023	2.731ValGln: 2.731 ± 0.02	3.052ValArg: 3.052 ± 0.016	4.403ValSer: 4.403 ± 0.02	3.446ValThr: 3.446 ± 0.031	4.081ValVal: 4.081 ± 0.029	0.561ValTrp: 0.561 ± 0.008	1.601ValTyr: 1.601 ± 0.014	0.002ValXaa: 0.002 ± 0.0
Trp	0.523TrpAla: 0.523 ± 0.007	0.185TrpCys: 0.185 ± 0.004	0.496TrpAsp: 0.496 ± 0.009	0.54TrpGlu: 0.54 ± 0.008	0.384TrpPhe: 0.384 ± 0.007	0.473TrpGly: 0.473 ± 0.008	0.253TrpHis: 0.253 ± 0.005	0.516TrpIle: 0.516 ± 0.008	0.555TrpLys: 0.555 ± 0.009	1.066TrpLeu: 1.066 ± 0.013	0.266TrpMet: 0.266 ± 0.005	0.483TrpAsn: 0.483 ± 0.008	0.37TrpPro: 0.37 ± 0.006	0.489TrpGln: 0.489 ± 0.007	0.632TrpArg: 0.632 ± 0.009	0.731TrpSer: 0.731 ± 0.011	0.551TrpThr: 0.551 ± 0.008	0.522TrpVal: 0.522 ± 0.008	0.155TrpTrp: 0.155 ± 0.005	0.313TrpTyr: 0.313 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.81TyrAla: 1.81 ± 0.013	0.648TyrCys: 0.648 ± 0.011	1.633TyrAsp: 1.633 ± 0.014	1.772TyrGlu: 1.772 ± 0.014	1.193TyrPhe: 1.193 ± 0.01	1.838TyrGly: 1.838 ± 0.017	0.76TyrHis: 0.76 ± 0.008	1.413TyrIle: 1.413 ± 0.014	1.524TyrLys: 1.524 ± 0.017	2.514TyrLeu: 2.514 ± 0.019	0.684TyrMet: 0.684 ± 0.008	1.399TyrAsn: 1.399 ± 0.014	1.335TyrPro: 1.335 ± 0.014	1.333TyrGln: 1.333 ± 0.012	1.627TyrArg: 1.627 ± 0.012	2.105TyrSer: 2.105 ± 0.016	1.563TyrThr: 1.563 ± 0.014	1.653TyrVal: 1.653 ± 0.014	0.326TyrTrp: 0.326 ± 0.007	1.035TyrTyr: 1.035 ± 0.011	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.002XaaGlu: 0.002 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.003XaaHis: 0.003 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.003XaaLeu: 0.003 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.003XaaPro: 0.003 ± 0.0	0.003XaaGln: 0.003 ± 0.0	0.002XaaArg: 0.002 ± 0.0	0.002XaaSer: 0.002 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.004XaaXaa: 0.004 ± 0.002

Statistics based on 19363 proteins (12990802 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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