Amino acid dipepetide frequency for Lachnoclostridium sp. An298

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.144AlaAla: 8.144 ± 0.138
1.128AlaCys: 1.128 ± 0.043
5.14AlaAsp: 5.14 ± 0.078
6.159AlaGlu: 6.159 ± 0.102
2.931AlaPhe: 2.931 ± 0.06
6.849AlaGly: 6.849 ± 0.107
1.121AlaHis: 1.121 ± 0.037
4.492AlaIle: 4.492 ± 0.075
4.354AlaLys: 4.354 ± 0.075
6.94AlaLeu: 6.94 ± 0.099
2.234AlaMet: 2.234 ± 0.053
2.219AlaAsn: 2.219 ± 0.048
2.125AlaPro: 2.125 ± 0.048
2.383AlaGln: 2.383 ± 0.061
3.266AlaArg: 3.266 ± 0.061
3.809AlaSer: 3.809 ± 0.077
2.763AlaThr: 2.763 ± 0.063
6.981AlaVal: 6.981 ± 0.099
0.609AlaTrp: 0.609 ± 0.028
2.791AlaTyr: 2.791 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
1.114CysAla: 1.114 ± 0.037
0.297CysCys: 0.297 ± 0.022
0.813CysAsp: 0.813 ± 0.033
0.921CysGlu: 0.921 ± 0.032
0.62CysPhe: 0.62 ± 0.028
1.591CysGly: 1.591 ± 0.047
0.31CysHis: 0.31 ± 0.018
1.141CysIle: 1.141 ± 0.037
0.641CysLys: 0.641 ± 0.029
1.19CysLeu: 1.19 ± 0.04
0.495CysMet: 0.495 ± 0.026
0.495CysAsn: 0.495 ± 0.022
0.704CysPro: 0.704 ± 0.026
0.413CysGln: 0.413 ± 0.023
0.955CysArg: 0.955 ± 0.036
0.959CysSer: 0.959 ± 0.034
0.803CysThr: 0.803 ± 0.031
1.078CysVal: 1.078 ± 0.035
0.119CysTrp: 0.119 ± 0.011
0.545CysTyr: 0.545 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
4.323AspAla: 4.323 ± 0.078
0.767AspCys: 0.767 ± 0.028
3.229AspAsp: 3.229 ± 0.074
5.078AspGlu: 5.078 ± 0.094
2.557AspPhe: 2.557 ± 0.06
4.51AspGly: 4.51 ± 0.085
1.0AspHis: 1.0 ± 0.033
4.775AspIle: 4.775 ± 0.08
3.317AspLys: 3.317 ± 0.07
4.784AspLeu: 4.784 ± 0.08
2.02AspMet: 2.02 ± 0.041
2.205AspAsn: 2.205 ± 0.044
2.087AspPro: 2.087 ± 0.044
1.556AspGln: 1.556 ± 0.04
2.933AspArg: 2.933 ± 0.064
3.23AspSer: 3.23 ± 0.07
3.421AspThr: 3.421 ± 0.076
3.965AspVal: 3.965 ± 0.07
0.586AspTrp: 0.586 ± 0.025
2.931AspTyr: 2.931 ± 0.064
0.0AspXaa: 0.0 ± 0.0
Glu
5.59GluAla: 5.59 ± 0.097
0.94GluCys: 0.94 ± 0.033
4.821GluAsp: 4.821 ± 0.085
7.843GluGlu: 7.843 ± 0.136
2.575GluPhe: 2.575 ± 0.056
4.522GluGly: 4.522 ± 0.079
1.403GluHis: 1.403 ± 0.043
5.787GluIle: 5.787 ± 0.087
6.736GluLys: 6.736 ± 0.098
6.85GluLeu: 6.85 ± 0.105
2.679GluMet: 2.679 ± 0.053
4.051GluAsn: 4.051 ± 0.073
2.11GluPro: 2.11 ± 0.053
3.093GluGln: 3.093 ± 0.073
3.814GluArg: 3.814 ± 0.074
3.548GluSer: 3.548 ± 0.068
4.035GluThr: 4.035 ± 0.076
4.359GluVal: 4.359 ± 0.073
0.73GluTrp: 0.73 ± 0.028
3.437GluTyr: 3.437 ± 0.069
0.0GluXaa: 0.0 ± 0.0
Phe
2.832PheAla: 2.832 ± 0.061
0.754PheCys: 0.754 ± 0.03
2.496PheAsp: 2.496 ± 0.058
2.469PheGlu: 2.469 ± 0.045
1.724PhePhe: 1.724 ± 0.05
3.039PheGly: 3.039 ± 0.057
0.808PheHis: 0.808 ± 0.029
2.775PheIle: 2.775 ± 0.058
1.692PheLys: 1.692 ± 0.044
3.758PheLeu: 3.758 ± 0.07
1.139PheMet: 1.139 ± 0.039
1.307PheAsn: 1.307 ± 0.039
1.42PhePro: 1.42 ± 0.039
1.19PheGln: 1.19 ± 0.039
2.053PheArg: 2.053 ± 0.054
2.735PheSer: 2.735 ± 0.06
2.36PheThr: 2.36 ± 0.054
2.772PheVal: 2.772 ± 0.06
0.415PheTrp: 0.415 ± 0.023
1.671PheTyr: 1.671 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
5.307GlyAla: 5.307 ± 0.095
1.31GlyCys: 1.31 ± 0.04
3.821GlyAsp: 3.821 ± 0.067
5.056GlyGlu: 5.056 ± 0.081
3.068GlyPhe: 3.068 ± 0.061
5.105GlyGly: 5.105 ± 0.087
1.272GlyHis: 1.272 ± 0.046
6.332GlyIle: 6.332 ± 0.088
5.186GlyLys: 5.186 ± 0.081
5.866GlyLeu: 5.866 ± 0.1
2.528GlyMet: 2.528 ± 0.05
3.1GlyAsn: 3.1 ± 0.064
1.382GlyPro: 1.382 ± 0.044
2.167GlyGln: 2.167 ± 0.049
3.703GlyArg: 3.703 ± 0.071
4.153GlySer: 4.153 ± 0.075
4.4GlyThr: 4.4 ± 0.071
5.048GlyVal: 5.048 ± 0.065
0.731GlyTrp: 0.731 ± 0.034
3.408GlyTyr: 3.408 ± 0.069
0.0GlyXaa: 0.0 ± 0.0
His
1.128HisAla: 1.128 ± 0.037
0.311HisCys: 0.311 ± 0.019
0.938HisAsp: 0.938 ± 0.032
1.149HisGlu: 1.149 ± 0.033
0.841HisPhe: 0.841 ± 0.029
1.322HisGly: 1.322 ± 0.042
0.404HisHis: 0.404 ± 0.024
1.473HisIle: 1.473 ± 0.041
0.838HisLys: 0.838 ± 0.03
1.547HisLeu: 1.547 ± 0.046
0.566HisMet: 0.566 ± 0.025
0.664HisAsn: 0.664 ± 0.023
0.928HisPro: 0.928 ± 0.034
0.481HisGln: 0.481 ± 0.024
0.881HisArg: 0.881 ± 0.031
0.961HisSer: 0.961 ± 0.032
0.98HisThr: 0.98 ± 0.034
1.141HisVal: 1.141 ± 0.032
0.165HisTrp: 0.165 ± 0.014
0.77HisTyr: 0.77 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
5.756IleAla: 5.756 ± 0.098
1.347IleCys: 1.347 ± 0.042
4.165IleAsp: 4.165 ± 0.06
4.835IleGlu: 4.835 ± 0.08
2.836IlePhe: 2.836 ± 0.06
5.17IleGly: 5.17 ± 0.085
1.371IleHis: 1.371 ± 0.034
4.916IleIle: 4.916 ± 0.081
3.544IleLys: 3.544 ± 0.064
7.036IleLeu: 7.036 ± 0.098
1.987IleMet: 1.987 ± 0.046
2.688IleAsn: 2.688 ± 0.057
3.256IlePro: 3.256 ± 0.062
2.207IleGln: 2.207 ± 0.055
4.55IleArg: 4.55 ± 0.067
4.803IleSer: 4.803 ± 0.076
4.06IleThr: 4.06 ± 0.074
4.843IleVal: 4.843 ± 0.091
0.649IleTrp: 0.649 ± 0.031
2.763IleTyr: 2.763 ± 0.062
0.0IleXaa: 0.0 ± 0.0
Lys
4.726LysAla: 4.726 ± 0.074
0.719LysCys: 0.719 ± 0.027
3.725LysAsp: 3.725 ± 0.062
6.058LysGlu: 6.058 ± 0.105
1.717LysPhe: 1.717 ± 0.043
3.919LysGly: 3.919 ± 0.075
0.973LysHis: 0.973 ± 0.034
4.598LysIle: 4.598 ± 0.078
5.621LysLys: 5.621 ± 0.093
4.764LysLeu: 4.764 ± 0.078
2.129LysMet: 2.129 ± 0.052
3.016LysAsn: 3.016 ± 0.054
1.803LysPro: 1.803 ± 0.041
1.975LysGln: 1.975 ± 0.044
3.229LysArg: 3.229 ± 0.061
3.119LysSer: 3.119 ± 0.063
3.372LysThr: 3.372 ± 0.057
3.938LysVal: 3.938 ± 0.068
0.646LysTrp: 0.646 ± 0.032
2.774LysTyr: 2.774 ± 0.055
0.0LysXaa: 0.0 ± 0.0
Leu
6.877LeuAla: 6.877 ± 0.089
1.555LeuCys: 1.555 ± 0.044
5.16LeuAsp: 5.16 ± 0.082
6.304LeuGlu: 6.304 ± 0.095
3.612LeuPhe: 3.612 ± 0.079
5.835LeuGly: 5.835 ± 0.092
1.536LeuHis: 1.536 ± 0.043
6.201LeuIle: 6.201 ± 0.095
5.864LeuLys: 5.864 ± 0.087
8.1LeuLeu: 8.1 ± 0.13
2.629LeuMet: 2.629 ± 0.051
3.551LeuAsn: 3.551 ± 0.053
3.536LeuPro: 3.536 ± 0.065
2.642LeuGln: 2.642 ± 0.053
4.095LeuArg: 4.095 ± 0.073
5.916LeuSer: 5.916 ± 0.082
5.072LeuThr: 5.072 ± 0.07
5.198LeuVal: 5.198 ± 0.077
0.735LeuTrp: 0.735 ± 0.031
3.397LeuTyr: 3.397 ± 0.074
0.0LeuXaa: 0.0 ± 0.0
Met
2.448MetAla: 2.448 ± 0.053
0.373MetCys: 0.373 ± 0.022
1.909MetAsp: 1.909 ± 0.042
2.637MetGlu: 2.637 ± 0.066
1.061MetPhe: 1.061 ± 0.04
1.991MetGly: 1.991 ± 0.05
0.507MetHis: 0.507 ± 0.024
2.259MetIle: 2.259 ± 0.049
2.563MetLys: 2.563 ± 0.057
2.721MetLeu: 2.721 ± 0.051
0.921MetMet: 0.921 ± 0.034
1.464MetAsn: 1.464 ± 0.039
1.205MetPro: 1.205 ± 0.032
1.017MetGln: 1.017 ± 0.037
1.513MetArg: 1.513 ± 0.044
1.86MetSer: 1.86 ± 0.043
1.872MetThr: 1.872 ± 0.048
1.718MetVal: 1.718 ± 0.05
0.232MetTrp: 0.232 ± 0.016
1.011MetTyr: 1.011 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
3.002AsnAla: 3.002 ± 0.05
0.578AsnCys: 0.578 ± 0.027
2.03AsnAsp: 2.03 ± 0.047
2.757AsnGlu: 2.757 ± 0.065
1.36AsnPhe: 1.36 ± 0.034
3.268AsnGly: 3.268 ± 0.064
0.727AsnHis: 0.727 ± 0.03
3.23AsnIle: 3.23 ± 0.063
2.088AsnLys: 2.088 ± 0.052
3.368AsnLeu: 3.368 ± 0.067
1.309AsnMet: 1.309 ± 0.038
1.519AsnAsn: 1.519 ± 0.041
1.861AsnPro: 1.861 ± 0.044
1.188AsnGln: 1.188 ± 0.039
2.132AsnArg: 2.132 ± 0.054
2.121AsnSer: 2.121 ± 0.05
2.3AsnThr: 2.3 ± 0.052
2.605AsnVal: 2.605 ± 0.055
0.392AsnTrp: 0.392 ± 0.02
1.708AsnTyr: 1.708 ± 0.052
0.0AsnXaa: 0.0 ± 0.0
Pro
2.815ProAla: 2.815 ± 0.06
0.468ProCys: 0.468 ± 0.02
2.668ProAsp: 2.668 ± 0.054
3.675ProGlu: 3.675 ± 0.062
1.51ProPhe: 1.51 ± 0.048
2.715ProGly: 2.715 ± 0.058
0.62ProHis: 0.62 ± 0.025
1.804ProIle: 1.804 ± 0.049
1.69ProLys: 1.69 ± 0.046
2.843ProLeu: 2.843 ± 0.052
0.895ProMet: 0.895 ± 0.032
1.129ProAsn: 1.129 ± 0.04
0.771ProPro: 0.771 ± 0.034
1.031ProGln: 1.031 ± 0.033
1.136ProArg: 1.136 ± 0.039
1.772ProSer: 1.772 ± 0.047
1.479ProThr: 1.479 ± 0.046
3.295ProVal: 3.295 ± 0.062
0.345ProTrp: 0.345 ± 0.019
1.455ProTyr: 1.455 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
2.408GlnAla: 2.408 ± 0.062
0.353GlnCys: 0.353 ± 0.022
1.589GlnAsp: 1.589 ± 0.039
2.785GlnGlu: 2.785 ± 0.061
1.167GlnPhe: 1.167 ± 0.036
1.912GlnGly: 1.912 ± 0.053
0.465GlnHis: 0.465 ± 0.022
2.714GlnIle: 2.714 ± 0.057
2.449GlnLys: 2.449 ± 0.058
2.521GlnLeu: 2.521 ± 0.051
1.178GlnMet: 1.178 ± 0.035
1.381GlnAsn: 1.381 ± 0.043
0.916GlnPro: 0.916 ± 0.032
1.121GlnGln: 1.121 ± 0.039
1.517GlnArg: 1.517 ± 0.045
1.668GlnSer: 1.668 ± 0.047
1.659GlnThr: 1.659 ± 0.047
1.968GlnVal: 1.968 ± 0.053
0.299GlnTrp: 0.299 ± 0.017
1.357GlnTyr: 1.357 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
3.161ArgAla: 3.161 ± 0.064
0.737ArgCys: 0.737 ± 0.032
2.51ArgAsp: 2.51 ± 0.06
4.44ArgGlu: 4.44 ± 0.082
2.023ArgPhe: 2.023 ± 0.049
2.844ArgGly: 2.844 ± 0.055
0.909ArgHis: 0.909 ± 0.03
4.016ArgIle: 4.016 ± 0.073
3.913ArgLys: 3.913 ± 0.071
4.523ArgLeu: 4.523 ± 0.072
1.804ArgMet: 1.804 ± 0.046
2.203ArgAsn: 2.203 ± 0.051
1.589ArgPro: 1.589 ± 0.051
1.985ArgGln: 1.985 ± 0.05
3.163ArgArg: 3.163 ± 0.062
2.486ArgSer: 2.486 ± 0.047
2.734ArgThr: 2.734 ± 0.055
2.804ArgVal: 2.804 ± 0.054
0.417ArgTrp: 0.417 ± 0.021
2.089ArgTyr: 2.089 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
4.428SerAla: 4.428 ± 0.078
0.807SerCys: 0.807 ± 0.03
3.596SerAsp: 3.596 ± 0.084
4.224SerGlu: 4.224 ± 0.071
2.448SerPhe: 2.448 ± 0.055
5.305SerGly: 5.305 ± 0.077
1.008SerHis: 1.008 ± 0.036
3.712SerIle: 3.712 ± 0.07
2.635SerLys: 2.635 ± 0.056
4.957SerLeu: 4.957 ± 0.088
1.719SerMet: 1.719 ± 0.043
1.793SerAsn: 1.793 ± 0.051
1.862SerPro: 1.862 ± 0.046
1.682SerGln: 1.682 ± 0.05
3.167SerArg: 3.167 ± 0.054
3.556SerSer: 3.556 ± 0.082
2.632SerThr: 2.632 ± 0.059
4.484SerVal: 4.484 ± 0.081
0.55SerTrp: 0.55 ± 0.027
2.411SerTyr: 2.411 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
4.639ThrAla: 4.639 ± 0.085
0.663ThrCys: 0.663 ± 0.031
3.609ThrAsp: 3.609 ± 0.064
4.166ThrGlu: 4.166 ± 0.074
2.03ThrPhe: 2.03 ± 0.051
5.108ThrGly: 5.108 ± 0.085
0.871ThrHis: 0.871 ± 0.033
3.508ThrIle: 3.508 ± 0.062
2.705ThrLys: 2.705 ± 0.061
4.659ThrLeu: 4.659 ± 0.073
1.428ThrMet: 1.428 ± 0.042
1.831ThrAsn: 1.831 ± 0.046
2.157ThrPro: 2.157 ± 0.048
1.447ThrGln: 1.447 ± 0.045
2.071ThrArg: 2.071 ± 0.049
2.849ThrSer: 2.849 ± 0.056
2.601ThrThr: 2.601 ± 0.057
4.622ThrVal: 4.622 ± 0.072
0.468ThrTrp: 0.468 ± 0.025
2.12ThrTyr: 2.12 ± 0.052
0.0ThrXaa: 0.0 ± 0.0
Val
4.355ValAla: 4.355 ± 0.082
1.325ValCys: 1.325 ± 0.041
3.801ValAsp: 3.801 ± 0.066
4.536ValGlu: 4.536 ± 0.089
3.06ValPhe: 3.06 ± 0.061
4.163ValGly: 4.163 ± 0.068
1.144ValHis: 1.144 ± 0.033
5.41ValIle: 5.41 ± 0.089
4.251ValLys: 4.251 ± 0.078
6.844ValLeu: 6.844 ± 0.101
2.126ValMet: 2.126 ± 0.052
2.713ValAsn: 2.713 ± 0.051
2.648ValPro: 2.648 ± 0.054
2.053ValGln: 2.053 ± 0.045
3.446ValArg: 3.446 ± 0.06
4.576ValSer: 4.576 ± 0.078
4.175ValThr: 4.175 ± 0.077
4.718ValVal: 4.718 ± 0.095
0.657ValTrp: 0.657 ± 0.028
2.707ValTyr: 2.707 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
0.532TrpAla: 0.532 ± 0.029
0.154TrpCys: 0.154 ± 0.013
0.559TrpAsp: 0.559 ± 0.027
0.655TrpGlu: 0.655 ± 0.026
0.455TrpPhe: 0.455 ± 0.023
0.664TrpGly: 0.664 ± 0.027
0.185TrpHis: 0.185 ± 0.017
0.692TrpIle: 0.692 ± 0.031
0.685TrpLys: 0.685 ± 0.032
0.886TrpLeu: 0.886 ± 0.033
0.356TrpMet: 0.356 ± 0.02
0.493TrpAsn: 0.493 ± 0.022
0.199TrpPro: 0.199 ± 0.015
0.38TrpGln: 0.38 ± 0.021
0.382TrpArg: 0.382 ± 0.021
0.492TrpSer: 0.492 ± 0.025
0.415TrpThr: 0.415 ± 0.021
0.484TrpVal: 0.484 ± 0.025
0.119TrpTrp: 0.119 ± 0.013
0.44TrpTyr: 0.44 ± 0.032
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.855TyrAla: 2.855 ± 0.058
0.616TyrCys: 0.616 ± 0.027
2.696TyrAsp: 2.696 ± 0.066
3.176TyrGlu: 3.176 ± 0.057
1.814TyrPhe: 1.814 ± 0.044
3.11TyrGly: 3.11 ± 0.069
0.854TyrHis: 0.854 ± 0.033
2.948TyrIle: 2.948 ± 0.056
2.049TyrLys: 2.049 ± 0.05
3.658TyrLeu: 3.658 ± 0.066
1.139TyrMet: 1.139 ± 0.036
1.72TyrAsn: 1.72 ± 0.045
1.555TyrPro: 1.555 ± 0.046
1.42TyrGln: 1.42 ± 0.04
2.344TyrArg: 2.344 ± 0.055
2.363TyrSer: 2.363 ± 0.057
2.419TyrThr: 2.419 ± 0.061
2.688TyrVal: 2.688 ± 0.062
0.363TyrTrp: 0.363 ± 0.021
1.931TyrTyr: 1.931 ± 0.065
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2948 proteins (916666 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski